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A multi-axis robot-based bioprinting system supporting natural cell function preservation and cardiac tissue fabrication

2001年

作者：（美）charlie Russel Sharon Crawford

来源：畅想之星图书光盘版（南... 评论

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Bioactive Materials 2022年第12期7卷 138-150页

作者： Zeyu Zhang Chenming Wu Chengkai Dai Qingqing Shi Guoxin Fang Dongfang Xie Xiangjie Zhao Yong-Jin Liu charlie CLWang Xiu-Jie Wang Institute of Genetics and Developmental Biology Innovation Academy of Seed DesignChinese Academy of SciencesBeijing100101China Beijing National Research Center for Information Science and Technology Department of Computer Science and TechnologyTsinghua UniversityBeijing100084China Department of Mechanical Aerospace and Civil EngineeringThe University of ManchesterManchesterM204BXUK Faculty of Industrial Design Engineering Delft University of TechnologyDelft2628the Netherlands University of Chinese Academy of Sciences Beijing100049China

Despite the recent advances in artificial tissue and organ engineering,how to generate large size viable and functional complex organs still remains as a grand challenge for regenerative ***-dimensional bioprinting has demonstrated its advantages as one of the major methods in fabricating simple tissues,yet it still faces difficulties to generate vasculatures and preserve cell functions in complex organ ***,we overcome the limitations of conventional bioprinting systems by converting a six degree-of-freedom robotic arm into a bioprinter,therefore enables cell printing on 3D complex-shaped vascular scaffolds from all *** also developed an oil bath-based cell printing method to better preserve cell natural functions after *** with a self-designed bioreactor and a repeated print-and-culture strategy,our bioprinting system is capable to generate vascularized,contractible,and long-term survived cardiac *** bioprinting strategy mimics the in vivo organ development process and presents a promising solution for in vitro fabrication of complex organs.

关键词： Six degree-of-freedom robot 3D bioprinting Artificial organ engineering Print-and-culture Cardiac tissue fabrication

Menstrual blood-derived mesenchymal stem cells differentiate into functional hepatocyte-like cells

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2013年第11期14卷 961-972页

作者： Xiao-zhou MOU Jian LIN Jin-yang CHEN Yi-fei LI Xiao-xing WU Bing-yu XIANG Cai-yun LI Ju-ming MA charlie XIANG State Key Laboratory for Diagnosis and Treatment of Infectious Diseases the First Affiliated HospitalSchool of MedicineZhejiang University Institute for Cell-Based Drug Development of Zhejiang Province People's Hospital of Tongling S-Evans Biosciences The 117th Hospital of PLA Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases

Orthotopic liver transplantation(OLT)is the only proven effective treatment for both end-stage and metabolic liver *** transplantation is a promising alternative for OLT,but the lack of available donor livers has hampered its clinical ***-like cells(HLCs)differentiated from many multi-potential stem cells can help repair damaged liver *** almost suitable cells currently identified for human use are difficult to harvest and involve invasive ***,a novel mesenchymal stem cell derived from human menstrual blood(MenSC)has been discovered and obtained easily and *** this study,we examined whether the MenSCs are able to differentiate into functional HLCs in *** three weeks of incubation in hepatogenic differentiation medium containing hepatocyte growth factor(HGF),fibroblast growth factor-4(FGF-4),and oncostain M(OSM),cuboidal HLCs were observed,and cells also expressed hepatocyte-specific marker genes including albumin(ALB),α-fetoprotein(AFP),cytokeratin 18/19(CK18/19),and cytochrome P450 1A1/3A4(CYP1A1/3A4).Differentiated cells further demonstrated in vitro mature hepatocyte functions such as urea synthesis,glycogen storage,and indocyanine green(ICG)*** intrasplenic transplantation into mice with 2/3 partial hepatectomy,the MenSC-derived HLCs were detected in recipient livers and expressed human ALB *** also showed that MenSC-derived HLC transplantation could restore the serum ALB level and significantly suppressed transaminase activity of liver injury *** conclusion,MenSCs may serve as an ideal,easily accessible source of material for tissue engineering and cell therapy of liver tissues.

关键词： Menstrual blood-derived mesenchymal stem cell(MenSC) Differentiation Hepatocyte Intrasplenic transplantation Partial hepatectomy

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玩转您的房屋

作者： (美)charlie Wing

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Let＇s　Share!

英语辅导（初三年级） 2005年第1期 59-59页

作者： charlie

读者吴晓梦：我想问一下，为什么学了很多新单词，但是写作文时仍习惯用一些旧的单词，新单词总是用不上。可以教一教我怎样在作文中用上新单词吗?

关键词：单词作文读者写作习惯

Becoming an emotionally healthy school

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1000年

作者： Smith charlie

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值得关注的两种债券

银行家 2002年第3期 140-142页

作者： charlie Ban 谭清香

市场上流通着由众多不同发行者发行的多种债券.债券有许多特征,诸如付息方式、债券发行市场、可支付通货、受保护性以及法律地位等.债券的发行者可以是政府、企业、专门的信托机构,甚至可以是非盈利组织.债券的种类通常是由发行者的类... 详细信息

市场上流通着由众多不同发行者发行的多种债券.债券有许多特征,诸如付息方式、债券发行市场、可支付通货、受保护性以及法律地位等.债券的发行者可以是政府、企业、专门的信托机构,甚至可以是非盈利组织.债券的种类通常是由发行者的类型和债券本身的特性所决定的,下面我们简单地讨论两种主要债券:

关键词：债券美元行权价格收益率本位币资产抵押债券资产担保证券

Comments　are　Welcome!

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英语辅导（初三年级） 2005年第1期 59-59页

作者： charlie

四川眉山陈星：我是一名初一的小小英文爱好者，我的词汇量很少，但是学习英语的兴趣很浓厚。前一段时间我买了很多英语课外书，但是因为很多单词都不认识，所以很难啃。是CE：Teens帮了我的大忙。虽然我只读了一期，但是我的词汇量有... 详细信息

四川眉山陈星：我是一名初一的小小英文爱好者，我的词汇量很少，但是学习英语的兴趣很浓厚。前一段时间我买了很多英语课外书，但是因为很多单词都不认识，所以很难啃。是CE：Teens帮了我的大忙。虽然我只读了一期，但是我的词汇量有了很大的提高，现在同学都叫我“活字典”。我已经能够自由自在地阅读世界名著了，

关键词：课外书爱好者英语课阅读兴趣词汇量单词字典英文同学

Seed Germination and Seedling Performance of Jatropha Curcas l. Fruit Based on Color at Two Different Seasons in Northern Philippines

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Seed Germination and Seedling Performance of Jatropha Curcas...

2011 International Conference on Environment and BioScience（ICEBS 2011）

作者： charlie B.Batin Mariano Marcos State University

The study characterized the fruit maturity of Jatropha curcas L., determined the fruit color that yields the highest oil content, and evaluated the seed germination and growth performance of Jatropha seedlings collected from Jatropha plantation in Lubbot, Batac City, Ilocos Norte. Changes in fruit color starting from the development of fruit until the color changes to black were documented for a period of 34 days. The experiment was laid out in Complete Randomized Design with four （4） treatments and three （3） replications. Treatments are as follows: T1-green （141D）, T2- yellow （11B）, T3- dark yellow and T4- black （202A）. The longest observable color transformation was observed in green （141D） to yellow （11B） color, while the shortest was exhibited by dark yellow （12B） to black （202A） color using the Royal Horticultural Color Chart （RHCC）. Seeds produced during the dry season had higher oil content than seeds during the wet season. Seeds taken from yellow fruits had the highest oil yield in both seasons. Germination and growth performance of Jatropha seedlings was significant for both seasons. Seeds taken from black colored fruits during the dry season and dark yellow for the rainy season were recommended for better germination and growth. These colors were found to produce higher seed quality and more vigorous seedlings compared to other fruit colors considered in the study.

关键词： Jatropha curcas L. biodiesel crop Germination value Relative Growth Rate Crude Fat Content

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Good　Suggestions!

英语辅导（初三年级） 2005年第1期 59-59页

作者： charlie

陕西西安高玮：CE：Teens的印刷很精美，但是有时候会由于过于注重版面设计而未能充分利用空间。希望你们能够减少图片，让内容更加充实。

关键词：初中写作指导书面表达学习辅导英语