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聚集体(英文) 2023年第3期4卷 162-168页

作者： Baoxing Shen Kwan ho Jung Songtao Ye Conner A.hoelzel charles h.wolstenholme he huang Yu Liu Xin Zhang Department of Chemistry School of Science and Research Center for Industries of the FutureWestlake Laboratory of Life Sciences and BiomedicineWestlake UniversityHangzhouChina School of Food Science and Pharmaceutical Engineering Nanjing Normal UniversityNanjingChina Department of Chemistry The Pennsylvania State UniversityUniversity ParkPennsylvaniaUSA Dalian Institute of Chemical Physics DalianChina Department of Biochemistry and Molecular Biology The Pennsylvania State UniversityUniversity ParkPennsylvaniaUSA

Aberrant protein aggregation leads to various human diseases,but little is known about the physical chemical properties of these aggregated proteins in ***,we developed a boron-dipyrromethene(BODIPY)-based haloTag probe,whose conjugation to haloTag-fused proteins allows us to study protein aggregates using both fluorescence intensity and *** of BODIPY fluorophore reveals key structural features to attain the dual *** optimized probe exhibits increased fluorescence intensity and elongated fluorescence lifetime in protein *** lifetime imaging using this probe indicates that protein aggregates afford different viscosity in the forms of soluble oligomers and insoluble aggregates in live *** strategy presented in this work can be extended to enable a wide class of haloTag probes that can be used to study a variety of physical properties of protein aggregates,thus helping unravel the pathogenic mechanism and develop therapeutic strategy.

关键词： fluorescence microscopy fluorescent probes protein aggregates

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Inverted Solvatochromic Stokes Shift in GFP-like Chromophores with Extended Conjugation

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Chinese Journal of Chemical Physics 2018年第4期31卷 599-607,616页

作者： hang hu charles h.wolstenholme Xin Zhang Xiaosong Li Department of Chemistry University of WashingtonSeattleWA 98195 Department of Chemistry The Pennsylvania State UniversityUniversity ParkPA 15802

Chrornophore structures inspired by natural green fluorescent protein （GFP） play an im- portant role in the field of bio-irnaging. In this work, photochemical properties of a new class of GFP-like chromophores are investigated using computational approaches. Thermo- dynamically stable isomers are identified in vacuum and in solvent. Spectral Stokes shifts are computed and compared to experiments. An inverted solvatochromic shift between absorption and emission emerging in this new class of GFP-like chromophores is observed, and attributed to the stabilized charge transfer and inhibited rotational structural reorganization in solvent.

关键词： Green fluorescent protein Solvatochrornic shift Stokes shift Time-dependentdensity functional theory Polarizable continuum model

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