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Replacing liquid fyrite with digital to improve <i>in vitro</i>fertilization laboratory quality control procedures

Open Journal of Obstetrics and Gynecology 2012年第1期2卷 7-10页

作者： Casey A. McDonald Zachary A. Haimowitz Andrea DeVenuta charity reeves Alan B. Copperman Jason Barritt Reproductive Endocrinology Mount Sinai School of Medicine New York USA Reproductive Medicine Associates of New York New York USA

In clinical in vitro fertilization (IVF), optimal culture conditions are required for production of high quality embryos and for achieving high pregnancy rates. Cell culture systems require vigilant attention to quality control and quality assurance, and upgrades to equipment and procedures require strenuous deliberation. During a 2-week maintenance period, we undertook an extensive analysis of incubator carbon dioxide (CO2) monitoring and the effect on culture media pH by comparing our traditional liquid Fyrite instruments to a certified and calibrated digital CO2 analyzer. The digital analyzer produced consistently lower CO2 readings and significantly greater precision than the liquid Fyrite. Media pH measurements showed significant variation depending on CO2 calibration device;however pH remained within manufacturers’ specifications. After superior performance by the digital analyzer, we incorporated this device into the incubator calibration and daily quality control procedures. A retrospective comparison of overall lab performance before and after this equipment switch demonstrated improved clinical pregnancy and implantation rates. This report illustrates the necessary caution when altering established laboratory procedures and equipment while highlighting the benefits of judiciously updating techniques and equipment in a laboratory setting that is often stubborn to change pre-existing, ingrained methodology.

关键词： IVF Incubator CO2 Laboratory Quality Control/Quality Assurance Liquid Fyrite Embryo Development Embryo Culture

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