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Engineered Bacillus subtilis alleviates intestinal oxidative injury through Nrf2-Keap1 pathway in enterotoxigenic Escherichia coli(ETEC) K88-infected piglet

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2023年第6期24卷 496-509页

作者： chaoyue WEN Hong ZHANG Qiuping GUO Yehui DUAN Sisi CHEN Mengmeng HAN Fengna LI Mingliang JIN Yizhen wang Institute of Feed Science Key Laboratory of Animal Feed and Nutrition of Zhejiang ProvinceKey Laboratory of Molecular Animal NutritionMinistry of EducationKey Laboratory of Animal Nutrition and Feed Science in Eastern ChinaMinistry of AgricultureCollege of Animal SciencesZhejiang UniversityHangzhou 310058China Hunan Provincial Key Laboratory of Animal Nutritional Physiology and Metabolic Process CAS Key Laboratory of Agro-Ecological Processes in Subtropical RegionNational Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry ProductionInstitute of Subtropical AgricultureChinese Academy of SciencesChangsha 410125China College of Advanced Agricultural Science University of Chinese Academy of SciencesBejing 100039China

Engineered probiotics can serve as therapeutics based on their ability of produce recombinant immune-stimulating *** this study,we built the recombinant Bacillus subtilis WB800 expressing antimicrobial peptide KR32(WB800-KR32)using genetic engineering methods and investigated its protective effects of nuclear factor-E2-related factor 2(Nrf2)-Kelch-like ECH-associated protein 1(Keap1)pathway activation in intestinal oxidative disturbance induced by enterotoxigenic Escherichia coli(ETEC)K88 in weaned ***-eight weaned piglets were randomly distributed into four treatment groups with seven replicates fed with a basal *** feed of the control group(CON)was infused with normal sterilized saline;meanwhile,the ETEC,ETEC+WB800,and ETEC+WB800-KR32 groups were orally administered normal sterilized saline,5×10^(10)CFU(CFU:colony forming units)WB800,and 5×10^(10)CFU WB800-KR32,respectively,on Days 1-14 and all infused with ETEC K881×10^(10)CFU on Days *** results showed that pretreatment with WB800-KR32 attenuated ETEC-induced intestinal disturbance,improved the mucosal activity of antioxidant enzyme(catalase(CAT),superoxide dismutase(SOD),and glutathione peroxidase(GPx))and decreased the content of malondialdehyde(MDA).More importantly,WB800-KR32 downregulated genes involved in antioxidant defense(GPx and SOD1).Interestingly,WB800-KR32 upregulated the protein expression of Nrf2 and downregulated the protein expression of Keap1 in the ***800-KR32 markedly changed the richness estimators(Ace and Chao)of gut microbiota and increased the abundance of Eubacterium_rectale_ATCC_33656 in the *** results suggested that WB800-KR32 may alleviate ETEC-induced intestinal oxidative injury through the Nrf2-Keap1 pathway,providing a new perspective for WB800-KR32 as potential therapeutics to regulate intestinal oxidative disturbance in ETEC K88 infection.

关键词： Engineered probiotics Intestine Oxidative injury Weaned piglets Nuclear factor-E2-related factor 2(Nrf2)-Kelch-like ECH-associated protein 1(Keap1)pathway

3D printed triboelectric nanogenerator as self-powered human–machine interactive sensor for breathing-based language expression

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Nano Research 2022年第8期15卷 7460-7467页

作者： Pengcheng Zhu Baosen Zhang Hongyi wang Yiheng Wu Hengjun Cao Liubing He chaoyue Li Xuepeng Luo Xing Li Yanchao Mao Key Laboratory of Materials Physics Ministry of EducationSchool of Physics and MicroelectronicsZhengzhou UniversityZhengzhou 450001China

Human–machine interfaces(HMIs)are important windows for a human to communicate with the outside *** current HMI devices such as cellphones,tablets,and computers can be used to help people with aphasia for language ***,these conventional HMI devices are not friendly to some particular groups who also lose their abilities of physical movements like in the intensive care unit(ICU)or vegetative patients to realize language ***,we report a breath-driven triboelectric nanogenerator(TENG)acting as a HMI sensor for language expression through human breathing without voice controls or manual *** TENG is integrated within a mask and fabricated via a three-dimensional(3D)printing *** wearing the mask,the TENG can produce responsive electric signals corresponding to the airflow from breathing,which is capable of recognizing human breathing types with different intensities,lengths,and *** the basis of the breathing recognition ability,a breathing-based language expressing system is further developed through introducing the Morse code as a communication *** with conventional language expressing devices,this system can extract subjective information of a person from breathing behaviors and output corresponding language text,which is not relying on voices or physical *** research for the first time introduces the self-powered breathing-based language expressing method to the field of HMI technology by using a 3D printed TENG,and could make HMI interactions become more friendly and fascinating.

关键词： self-powered sensor triboelectric nanogenerator human-machine interface breathing-based language expression

Effects of Y and Zn additions on electrical conductivity and electromagnetic shielding effectiveness of Mg-Y-Zn alloys

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Journal of Materials Science & Technology 2019年第6期35卷 1074-1080页

作者： Lizi Liu Xianhua Chen Jingfeng wang Liying Qiao Shangyu Gao Kai Song chaoyue Zhao Xiaofang Liu Di Zhao Fusheng Pan College of Materials Science and Engineering Chongqing University Chongqing 400045 China National Engineering Research Center for Magnesium Alloys Chongqing University Chongqing 400045 China Chongqing Academy of Science and Technology Chongqing 401123 China

Microstructure, electrical conductivity, and electromagnetic interference(EMI) shielding effectiveness(SE) of cast Mg-x Zn-y Y(x = 2–5, y = 1–10) alloys were systematically investigated to understand the effects of Zn and Y additions on electrical conductivity and electromagnetic shielding effectiveness of the *** results indicate that the electrical conductivity and SE of the Mg-x Zn-y Y alloys decrease with Y/Zn ratio. Electrical conductivity is the main factor that affects the electromagnetic shielding properties and the variation tendency of electromagnetic shielding properties of the Mg-x Zn-y Y alloys is consistent with conductivity. Valence of Y and Zn atoms, configuration of extranuclear electron and volumetric difference are main reasons for the variations in the electrical conductivity. A high density of second phase and the formation of semi-continuous network structure can also improve the SE value at high frequencies.

关键词： Mg-Zn-Y alloy Electrical conductivity Electromagnetic shielding effectiveness

Comparison of the meat quality and fatty acid profile of muscles in finishing Xiangcun Black pigs fed varied dietary energy levels

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Animal Nutrition 2022年第4期11卷 15-24页

作者： Can Yang Wenlong wang Xiaowu Tang Ruilin Huang Fengna Li Wenxuan Su Yulong Yin chaoyue Wen Jian Liu College of Life Sciences and Environment Hunan Provincial Key Laboratory of Biological Resources Protection and Utilization in Nanyue Mountain AreaHengyang Normal UniversityHengyangHunan 421008China Key Laboratory of Agro-ecological Processes in Subtropical Region National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry ProductionInstitute of Subtropical AgricultureChinese Academy of ScienceChangsh1Hunan 410125China Hunan International Joint Laboratory of Animal Intestinal Ecology and Health Hunan Normal UniversityChangshaHunan 410081China College of Bioengineering Hunan Vocational Technical College of Environment and BiologyHengyangHunan 421005China Key Laboratory of Animal Feed and Nutrition of Zhejiang Province College of Animal SciencesZhejiang UniversityHangzhou 310058China Xiangcun High-technology Agricultural Co.LTD LoudiHunan 417000China

To study the effects of dietary energy level on the meat quality of different muscles in finishing pigs,400Xiangcun Black pigs(BW=79.55±4.77 kg)were randomly assigned to 5 treatments with varied calculated digestive energy(DE)at 3,050,3,100,3,150,3,200 and 3,250 kcal/kg,*** treatment had 8replicates with 10 pigs per *** quality,amino acid and fatty acid composition were tested in this *** differences in average daily gain,average daily feed intake or feed-to-gain ratio(P>0.05)were observed among dietary *** concentrations of longissimus dorsi(LD)muscle in DE3150was higher than those in other groups(P<0.05).The crude fat concentration of biceps femoris(BF)muscle in DE3250 tended to be higher than that in DE3150 and DE3100 groups(P<0.05).Pigs in DE3250 and DE3200 had higher fiber density and smaller cross-sectional area of BF muscle than those in DE3150(P<0.05).Pigs in DE3150 had the highest Cu concentration in LD muscle compared with those in DE3200,DE3250(P<0.05).The C16:1 proportion of LD muscle was lower(P<0.01)and C20:1 was higher(P<0.05)in DE3050 than that in the other dietary *** C18:3n6 and C20:3n6 proportions of BF muscle in DE3150 were higher than those in DE 3050,DE3200 and DE3250(P<0.05).For LD muscle,m RNA expressions of type I and IIa MyHC in group DE3150 were higher than other treatments(P<0.01).The LD muscle in DE3150 expressed higher PPARd than in other groups(P<0.01).Pigs in DE3100 expressed higher FOX1 than in DE3200 and DE3250(P<0.05).Sterol-regulatory element binding proteins(SREBPa)mRNA expression decreased linearly when dietary energy level increased in BF muscle(P<0.01).In conclusion,a200 kcal/kg decrease in digestible energy for 4 consecutive weeks did not affect growth performance of Xiangcun Black ***,LD and BF muscle respond differently to dietary energy level,and meat quality was improved by the medium energy level during the finishing phase.

关键词： Energy Growth performance Fatty acid Myofibrillar morphology Muscle

Local atomic structure evolution of liquid gadolinium and yttrium during solidification:An ab initio study

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Journal of Rare Earths 2023年第8期41卷 1265-1271,I0006页

作者： Qinghe Zheng Tao Hu Sébastien Le Roux Musen Li chaoyue Chen Jianbo Yu Jiang wang Wei Ren Zhongming Ren State Key Laboratory of Advanced Special Steels School of Materials Science and EngineeringDepartment of PhysicsInternational Center of Quantum and Molecular StructuresShanghai UniversityShanghai200444China University of Strasbourg Institut de Physique et Chimie des Matériaux de StrasbourgCNRSUMR 750423 rue du LoessStrasbourgF-67034France

Local atomic structure evolution of pure gadolinium(Gd)and yttrium(Y)during solidification was investigated by using ab initio molecular dynamics(AIMD)*** calculated results indicate that the local short-range order(SRO)in liquid Gd and Y is similar to some transitional metals with an asymmetric shape of the second peak in static structure ***,the formation of icosahedral local motifs as a function of temperature decreases the diffusivity,which explains the connection between structure evolution and dynamic *** examining the topological structures of both systems,we demonstrate that small atomic displacement leads to two different types of topological sixfold rings in liquid and solid *** analyses yield a systematic study about rare earth metals Gd and Y at the atomic level.

关键词： Atomic structure evolution Rare earth Ab initio molecular dynamics Solidification

Design of ZnSe-CoSe heterostructure decorated in hollow N-doped carbon nanocage with generous adsorption and catalysis sites for the reversibly fast kinetics of polysulfide conversion

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Journal of Energy Chemistry 2023年第11期86卷 135-145,I0004页

作者： Junan Feng Chuan Shi Hanghang Dong chaoyue Zhang Wendong Liu Yu Liu Tianyi wang Xiaoxian Zhao Shuangqiang Chen Jianjun Song College of Physics Qingdao UniversityQingdao 266071ShandongChina College of Chemistry and Chemical Engineering Qingdao UniversityQingdao 266071ShandongChina Department of Chemical Engineering School of Environmental and Chemical EngineeringShanghai UniversityShanghai 200444China School of Chemistry and Chemical Engineering Yangzhou UniversityYangzhou 225002JiangsuChina Department of Chemistry College of ScienceHebei Agricultural UniversityBaoding 071001HebeiChina

Although lithium-sulfur batteries(Li SBs)are regarded as one of the most promising candidates for the next-generation energy storage system,the actual industrial application is hindered by the sluggish solid–liquid phase conversion kinetics,severe shuttle effect,and low sulfur ***,a zeolitic imidazolate framework(ZIF)derived heterogeneous ZnSe-CoSe nanoparticles encapsulated in hollow N-doped carbon nanocage(ZnSe-CoSe-HNC)was designed by etching with tannic acid as a multifunctional electrocatalyst to boost the polysulfide conversion kinetics in *** hollow structure in ZIF ensures large inner voids for sulfur and buffering volume *** exposed ZnSe-CoSe heterogeneous interfaces serve as bifunctional adsorption-catalytic centers to accelerate the conversion kinetics and alleviate the shuttle *** with the highly conductive framework,the ZnSe-CoSeHNC/S cathode exhibits a high initial reversible capacity of 1305.3 m A h g-1at 0.2 C,high-rate capability,and reliable cycling stability under high sulfur loading and lean electrolyte(maintaining at 745 m A h g-1after 200 cycles with a high sulfur loading of 6.4 mg cm-2and a low electrolyte/sulfur ratio of 6μL mg^(-1)).Theoretical calculations have demonstrated the heterostructures of ZnSe-CoSe offer higher binding energy to lithium polysulfides than that of ZnSe or CoSe,facilitating the electron transfer to lithium *** work provides a novel heterostructure with superior catalytic ability and hollow conductive architecture,paving the way for the practical application of functional sulfur electrodes.

关键词： Lithium-sulfur batteries Heterostructure Conversion Kinetics Hollow structure Bi-directional catalysis

Twinning,phase boundary structure and development of high coercivity in Fe-rich Sm_(2)Co_(17)-type magnets

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Journal of Rare Earths 2022年第1期40卷 102-111页

作者： Haichen Wu Zhuang Liu chaoyue Zhang Qiqi Yang Huanming Lu Guoxin Chen Xinming wang Yong Li Renjie Chen Aru Yan Analytical Center Ningbo Institute of Materials Technology and EngineeringChinese Academy of SciencesNingbo 3 J5201China Key Laboratory of Magnetic Materials and Devices Ningbo Institute of Materials Technology and EngineeringChinese Academy of SciencesNingbo 315201China

The microstructure of twinning as well as the phase boundary between 1:5 H and 2:17 R phase in Fe-rich Sm_(2)Co_(17)-type magnets was characterized at atomic scale using nanobeam diffraction and highresolution STEM-HAADF imaging,and the reason for the dramatic increase of coercivity during slow cooling was investigated based on the microchemistry *** twinning relationship in the 2:17 R phase originates from ordered substitution of Sm atoms by Co-Co atomic pairs on every three(3033)and(3033)planes,leading to formation of two corresponding equivalent twin *** basal plane of the 2:17 R phase,the 1:3 R platelet phase across the 2:17 R cell and the 1:5 H cell boundary phase between two adjacent 2:17 R cells all can act as effective twin *** cell boundary phase is precipitated along the pyramidal habit plane,and a fully coherent phase boundary(PB)is formed between the 1:5 H and 2:17 R phases with the orientation relationship to be PB//(1121)1:5 H//(1011)_(2):17 *** phase boundary may either be parallel to or intersect with the pyramidal planes occupied by Co-Co atomic *** substantial increase of coercivity during slow cooling is ascribed to the development of large gradient of the elements concentration within the cell boundary phase,resulting in large gradient of domain wall energy,and thus the pinning strength of the cell boundary phase against magnetic domain wall motion is significantly enhanced.

关键词： Sm_(2)Co_(17)-type magnets Microstructure Twinning Phase boundary Coercivity Rare earths

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新形势下我国抽水蓄能技术新突破与前景展望

水电与抽水蓄能 2023年第6期9卷 15-19页

作者：路振刚叶宏梁廷婷徐亚楠张晨谭学奇安小丹王超跃国网新源控股有限公司北京市100761 天津大学水利工程智能建设与运维全国重点实验室天津市300350 中国电力出版社有限公司北京市100005

近年来,抽水蓄能建设经历了新的实践和挑战,促进了工程技术的新发展、新理念和新认识,我国在该领域积累了丰富的工程技术经验。为进一步总结新形势下我国抽水蓄能成套设备自主化实践新突破,本文回顾了我国抽水蓄能电站发展历程,介绍了... 详细信息

近年来,抽水蓄能建设经历了新的实践和挑战,促进了工程技术的新发展、新理念和新认识,我国在该领域积累了丰富的工程技术经验。为进一步总结新形势下我国抽水蓄能成套设备自主化实践新突破,本文回顾了我国抽水蓄能电站发展历程,介绍了抽水蓄能技术新突破并探讨了其未来的发展方向。未来抽水蓄能应在建设高水头、大容量、变速抽水蓄能机组基础上,大力开展数字化智能抽水蓄能电站、大型交流励磁变速抽水蓄能机组、中小容量全功率变频抽水蓄能机组的可行性研究,更好地发挥抽水蓄能在实现“碳达峰”“碳中和”目标、推进能源清洁低碳转型等方面的关键作用。

关键词：抽水蓄能技术突破展望

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Bacillus amyloliquefaciens 40 regulates piglet performance,antioxidant capacity,immune status and gut microbiota

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Animal Nutrition 2023年第1期12卷 116-127页

作者： Zipeng Jiang Weifa Su Wentao Li chaoyue Wen Shuai Du Huan He Yu Zhang Tao Gong Xinxia wang Yizhen wang Mingliang Jin Zeqing Lu National Engineering Research Centre for Green Feed and Healthy Farming Zhejiang UniversityHangzhouChina Key Laboratory of Molecular Nutrition Ministry of EducationZhejiang UniversityHangzhouChina Key Laboratory of Animal Nutrition and Feed Ministry of Agriculture and Rural AffairsZhejiang UniversityHangzhouChina Key Laboratory of Animal Nutrition and Feed Nutrition of Zhejiang Province Zhejiang UniversityHangzhouChina College of Animal Science Institute of Feed ScienceZhejiang UniversityHangzhouChina

Probiotics can improve animal growth performance and intestinal *** species,Lactobacillus species,Bifidobacterium species,yeast *** the common types of ***,understanding the effects of probiotics on the immune status and gut microbiota of weaning piglets and how the probiotics exert their impact are still *** study aimed to investigate the effects of Bacillus amyloliquefaciens 40(BA40)on the performance,immune status and gut microbiota of piglets.A total of 12 litters of newborn piglets were randomly divided into 3 *** in control group were orally dosed with phosphate buffered saline;BA40 group and probiotics group were orally gavaged with resuspension BA40 and a probiotics product,*** results showed that BA40 treatment significantly decreased(P<0.05)the diarrhea incidence(from d 5 to 40),diamine oxidase,D-lactate,interleukin(IL)-1βand interferon-γconcentrations compared with control group and probiotics *** BA40 dramatically increased the total antioxidant capacity,IL-10 and secretory immunoglobulin-A concentrations in contrast to control *** the microbial composition,BA40 modulated the microbiota by improving the abundance of Bacteroides,Phascolarctobacterium(producing short-chain fatty acids)and Desulfovibrio and reducing the proliferation of pathogens(Streptococcus,Tyzzerella,Vellionella and paraeggerthella).Meanwhile,a metabolic function prediction explained that carbohydrate metabolism and amino acid metabolism enriched in BA40 group in contrast to control group and probiotics *** correlation analysis,the results demonstrated that BA40-enriched Phascolarctobacterium and Desulfovibrio provide insights into strategies for elevating the health status and performance of weaned ***,BA40 exerted stronger ability in decreasing diarrhea incidence and improved antioxidant activity,gut barrier function and immune status of piglets than the other *** study pr

关键词： Bacillus amyloliquefaciens Piglet Immune status Gut microbiota Metabolic function