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Expression of inducible nitric oxide synthase in trophoblasts and deciduas in early medical abortion

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Frontiers of Medicine 2009年第2期3卷 216-219页

作者： Geqing XIA chaoying wu Department of Obstetrics&Gynecology Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430022China

The purpose of this study was to investigate the expression of inducible nitric oxide synthase(iNOS)in trophoblasts and deciduas in early medical abortion,and study the relationship of medical abortion through mifepristone and iNOS in early *** of iNOS in trophoblasts and deciduas was detected by both in situ hybridization and immunohistochemical assay in 40 patients(experimental group);the positive expression of iNOS was represented by number density(N/S)and positive unit(Pu)using computer color magic image analysis system(CMIAS).All results were compared with those obtained from vacuum *** the experi-mental group,N/S and Pu in trophoblasts were 0.120�0.010 and 15.3�2.6,respectively,while in the control group,they were 0.021�0.003 and 3.1�0.5,respectively,and there were signiﬁcant differences between the two *** immunohistochemical assay,N/S and Pu were 0.090�0.010,10.24�1.55 vs 0.016�0.002,1.26�0.33 in the trophoblasts of the two groups;there were also signiﬁcant differences between the two *** were lower iNOS expression in deciduas by in situ hybridization and immunohistochem-ical assay,and the difference between the two groups was not signiﬁ*** was concluded that mifepristone induced medical abortion through the expression of iNOS in trophoblasts but not in deciduas.

关键词： mifepristone inducible nitric oxide synthase early pregnancy placental immunity

Zebrafish ppp1r21 mutant as a model for the study of primary biliary cholangitis

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Journal of Genetics and Genomics 2023年第12期50卷 1004-1013页

作者： chaoying wu Wenfeng Zhang Yiyu Luo Chaoqing Cheng Xinjuan Wang Yan Jiang Shuang Li Lingfei Luo Yun Yang Institute of Developmental Biology and Regenerative Medicine Southwest UniversityBeibeiChongqing 400715China

Primary biliary cholangitis (PBC) is an autoimmune cholestatic liver disease that progresses to fibrosis and cirrhosis, resulting from the gradual destruction of intrahepatic bile ducts. Exploring genetic variants associated with PBC is essential to understand the pathogenesis of PBC. Here we identify a zebrafish balloon dog (blg) mutant with intrahepatic bile duct branching defects, exhibiting several key pathological PBC-like features, including immunodominant autoantigen PDC-E2 production, cholangiocyte apoptosis, immune cell infiltration, inflammatory activation, and liver fibrosis. blg encodes the protein phosphatase 1 regulatory subunit 21 (Ppp1r21), which is enriched in the liver and its peripheral tissues and plays a vital role in the early intrahepatic bile duct formation stage. Further studies show an excessive activation of the PI3K/AKT/mTOR pathway in the hepatic tissues in the mutant, while treatment with the pathway inhibitor LY294002 and rapamycin partially rescues intrahepatic bile duct branching defects and alleviates the PBC-like symptoms. These findings implicate the potential role of the Ppp1r21-mediated PI3K/AKT/mTOR pathway in the pathophysiology of PBC.

关键词： Zebrafish mutant ppp1r21 Primary biliary cholangitis PDC-E2 PI3K/AKT/mTOR pathway

Study on the Supply Efficiency of Rural Public Service in China Based on Three-stage DEA Model

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Asian Agricultural Research 2014年第1期6卷 6-13页

作者： Zongbing DENG chaoying wu Junliang Zhang Ju WANG The Research Center of Rural Economy and Management Southwest University College of Economics and Management Southwest University

Improving the supply efficiency of rural public service is an important way to solve the severe shortage of rural public service. In this article,we use three-stage DEA model to carry out empirical research of the supply efficiency of rural public service in 31 provinces and regions of China. The results show that if without control over exogenous environment variables and random brunt,the classic DEA method will overestimate the rural public service efficiency; after controlling the impact of the external environmental factors,the mean of supply efficiency of rural public service in 31 provinces and regions of China is 0. 697; the improved rural per capita income,population density,population size and the educational level of residents,is a significantly favorable factor for enhancing the supply efficiency of rural public service,while the increase in the proportion of fiscal spending on rural public service to GDP plays no significant role in improving the rural public service efficiency; according to their efficiency type,the provinces and regions should adopt some measures,such as improving the management level or expanding the supply scale,to improve the supply efficiency.

关键词： Rural public service Supply efficiency Influencing

载脂蛋白E通过激活ERK/MMP9信号通路促进子宫内膜癌细胞的迁移

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南方医科大学学报 2023年第2期43卷 232-241页

作者：吴超英程文俊南京医科大学常州医学中心常州市妇幼保健院妇科江苏常州213000 南京医科大学第一附属医院妇科江苏南京210029

目的研究载脂蛋白E(APOE)在子宫内膜癌转移中的具体作用及分子调控机制。方法子宫内膜癌HEC-1B细胞常规培养,空白对照组siRNA以及APOE靶向的siRNA转染子宫内膜癌HEC-1B细胞,以及采用空载质粒和APOE过表达质粒转染子宫内膜癌HEC-1B细胞... 详细信息

目的研究载脂蛋白E(APOE)在子宫内膜癌转移中的具体作用及分子调控机制。方法子宫内膜癌HEC-1B细胞常规培养,空白对照组siRNA以及APOE靶向的siRNA转染子宫内膜癌HEC-1B细胞,以及采用空载质粒和APOE过表达质粒转染子宫内膜癌HEC-1B细胞,分为空白对照组siCtrl、siAPOE组、空载质粒组、APOE过表达质粒组。利用划痕实验和Transwell实验检测子宫内膜癌细胞的迁移能力,通过MTT实验及流式细胞术验证APOE对子宫内膜癌细胞增殖能力的影响,使用Hoechst染色法检测干扰APOE后HEC-1B细胞的凋亡情况,采用qPCR、Western blotting观察ERK/MMP9信号通路的变化;通过免疫组化的方法分析APOE的表达与子宫内膜癌组织分化程度的相关性。结果划痕实验和Transwell小室实验结果显示,与空白对照组相比,转染了siAPOE的HEC-1B细胞的迁移能力均明显下降(P<0.05)。与空载质粒组相比,过表达APOE能显著促进子宫内膜癌细胞HEC-1B的迁移能力(P<0.05)。MTT实验及流式细胞术结果显示APOE对细胞增殖无影响。Hoechst染色实验结果表明干扰APOE后HEC-1B细胞的凋亡能力没有受到影响。Western blotting结果显示敲低APOE能够降低子宫内膜癌细胞中MMP9的蛋白表达水平(P<0.05),而过表达APOE能使细胞内MMP9的蛋白水平升高(P<0.05);U0126能够抑制过表达APOE对MMP9的促进作用(P<0.05)。免疫组化结果显示,APOE在子宫内膜癌组织的表达水平比癌旁组织高。结论 APOE在子宫内膜癌组织中呈高表达,APOE可通过影响ERK的磷酸化,促进子宫内膜癌细胞中MMP9的表达,促进子宫内膜癌细胞的迁移。

关键词： APOE ERK 子宫内膜癌细胞迁移

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新闻工作者的职业道德和个人操守

青海社会科学 2006年第6期 147-149页

作者：任庆吴朝瑛青海日报社总编室青海省新闻工作者协会

在我国现阶段新闻工作实践中,广大新闻工作者的职业道德素质总体上是好的,但在某些方面还存在着明显的问题。本文在强调新闻工作者必须具备良好职业道德和修养的重要性的同时,就如何加强新闻工作者的职业道德和个人操守,阐述了自己的观点。

关键词：新闻工作者职业道德个人操守

Expression of Toll-like Receptor 4 in Neonatal Cord Blood Mononuclear Cells in Patients with Preeclampsia

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Journal of Huazhong University of Science and Technology(Medical Sciences) 2010年第5期30卷 615-619页

作者：夏革清徐丹芬吴敏吴超英 Department of Obstetrics and Gynecology Union HospitalTongji Medical CollegeHuazhong University of Science and Technology

The expression of Toll-like receptor 4 (TLR4) in neonatal cord blood mononuclear cells (MNCs) and serum TNF-α were investigated in order to explore the roles of TLR4 in the pathogenesis of *** study enrolled 27 patients suffering from preeclampsia (experimental group) and 21 normal pregnancy patients (control group).After MNCs were separated, the expression of TLR4 mRNA and protein was detected by using real-time quantitative PCR and Western blotting respectively, and the expression of TNF-α by using *** results showed the TLR4 mRNA level in cord blood MNCs (2-CT:0.07±0.17), TLR4 protein expression level (absorbance ratio:0.81%±0.15%) and TNF-α level (9.5±1.73 pg/mL) were all increased in experimental group as compared with control group with the differences being statistically significant (P<0.05).There was a positive correlation between the expression of TLR4 mRNA and TNF-α in both experimental group and control group (r=0.54 and 0.53, respectively, P<0.05).It was concluded that TLR4 expression in the experimental group of cord blood MNCs was increased and there was a positive correlation between the expression of TLR4 mRNA and TNF-α in both ***4-mediated release of inflammatory cytokines may be one of the important reasons leading to preeclampsia.

关键词： preeclampsia mononuclear cells Toll-like receptor 4 tumor necrosis factor α

论法秩序统一原理视野下假冒专利罪的成立范围

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法学杂志 2023年第4期44卷 131-142页

作者：王志祥吴超莹北京师范大学刑事法律科学研究院北京100875

为了更好地保护专利权,在宏观层面,站在专利权保护的整体立场,应当正确理解专利权刑法保护的内涵。从法秩序统一的视角出发,根据前置法与后置法之间的关系,刑法对专利的保护应当受到前置法所确立的专利权的制约。各部门法应当做到相互... 详细信息

为了更好地保护专利权,在宏观层面,站在专利权保护的整体立场,应当正确理解专利权刑法保护的内涵。从法秩序统一的视角出发,根据前置法与后置法之间的关系,刑法对专利的保护应当受到前置法所确立的专利权的制约。各部门法应当做到相互合作、各有侧重;而作为法益最后保护手段的刑法则应当保持谦抑,审慎适用。假冒专利罪的保护法益是复合法益,其中包括个人法益和超个人法益。超个人法益的内容是国家专利管理秩序和专利标示秩序。在认定假冒他人专利行为时,专利权的存在是专利刑法保护之前提。伪造或变造专利申请文件的行为和面向特定对象的“假冒他人专利”的行为不应当被认定为构成假冒专利罪。

关键词：假冒专利罪法秩序统一原理刑法谦抑性超个人法益

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迭代开发中需求管理技术的研究与应用

微计算机信息 2008年第3期24卷 191-193页

作者：吕潇吴超英王倩北京航空航天大学计算机学院北京100083

迭代开发能够有效降低项目风险,适应客户需求变更,已被广泛采用。但是,如果不对多次迭代涌现的需求进行有效管理,必将严重降低迭代开发项目的成功率。本文对迭代开发中的需求管理方法进行研究,并基于研究给出了一个系统实现的框架。

关键词：需求管理迭代开发需求追踪

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如何加强环境监测质量管理体系建设

中国资源综合利用 2017年第8期35卷 86-88页

作者：吴朝英四川劳研科技有限公司四川攀枝花617000

近年来,虽然我国经济一直保持了较快的发展速度,但是自然生态环境也承受了巨大的压力,并遭受了严重的破坏。环境的破坏给人们的生存、发展带来了严重威胁,人们必须有效地解决环境问题,加强环境监测质量管理体系的建设,及时发现环境问题... 详细信息

近年来,虽然我国经济一直保持了较快的发展速度,但是自然生态环境也承受了巨大的压力,并遭受了严重的破坏。环境的破坏给人们的生存、发展带来了严重威胁,人们必须有效地解决环境问题,加强环境监测质量管理体系的建设,及时发现环境问题并做好处理就显得十分重要。因此,本文将针对如何加强环境监测质量管理体系的建设进行分析。

关键词：环境监测质量管理体系建设员工素质