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Interference of CsGPA1,theα-submit of G protein,reduces drought tolerance in cucumber seedlings

Horticultural Plant Journal 2021年第3期7卷 209-220页

作者： Yumei Liu Qinghua Du Longqiang Bai Mintao Sun Yansu Li chaoxing he Jun Wang Xianchang Yu Yan Yan The Institute of Vegetables and Flowers Chinese Academy of Agricultural SciencesBeijing 100081China College of Agricultural and Biological Engineering Heze UniversityHezeShandong 274000China Tianjin Agricultural University Tianjin 300384China School of Horticulture Shanxi Agricultural UniversityTaiguShanxi 030801China

The G proteinα-subunit,GPA1,is an integral component of several signaling pathways in plants,including response to abiotic ***,the molecular mechanism behind these processes remains largely unknown in the cucumber plant(Cucumis sativus L.).In order to further understand the role of CsGPA1 in cucumber under drought stress,changes in plant growth,physiological parameters,and gene expression of CsAQPs were all measured under water stress induced by polyethylene glycol(PEG)using wild type(WT)and CsGPA1-interference(RNAi)cucumber *** results demonstrated that the RNAi plants had lower drought tolerance,displaying seriously withered leaves,lower relative growth rate,lower root-shoot ratio,and lower root activity under drought stress compared to WT *** studies indicated that the suppression of CsGPA1 weakened drought stress tolerance due to higherwater loss rate in the leaves,higher levels of hydrogen peroxide(H2O2),increased malondialdehyde(MDA)content,lower free proline content,lower soluble sugar content,lower soluble protein content,and decreased antioxidant enzyme ***-PCR analysis demonstrated that the interference of CsGPA1 up-regulated the expression of most AQP genes(except for CsPIP2;3 in leaves)and down-regulated the expression of CsPIP1;2,CsPIP1;4,CsPIP2;1,and CsPIP2;4 in roots under drought stress when compared to WT *** results demonstrated that CsGPA1 could function as a positive regulator in drought stress response by decreasing the accumulation of reactive oxygen species(ROS),improving permeable potentials,and reducing water loss in cucumber plants.

关键词： Cucumis sativus CsGPA1 Drought tolerance ROS scavenging Water content

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Multifaceted regulatory functions of CsBPC2 in cucumber under salt stress conditions

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Horticulture Research 2023年第5期10卷 126-138页

作者： Shuzhen Li Mintao Sun Li Miao Qinghua Di Lijun Lv Xianchang Yu Yan Yan chaoxing he Jun Wang Aokun Shi Yansu Li State Key Laboratory of Vegetable Biobreeding Institute of Vegetables and FlowersChinese Academy of Agricultural SciencesBeijing 100081China Ganzhou Key Laboratory of Greenhouse Vegetable College of Life ScienceGannan Normal UniversityGanzhou 341000China Collaborative Innovation Center for Efficient and Green Production of Agriculture in Mountainous Areas of Zhejiang Province College of Horticulture ScienceZhejiang Agriculture and Forestry UniversityHangzhou 311300ZhejiangChina

BASIC PENTACYSTEINE(BPC)transcription factors are essential regulators of plant growth and ***,BPC functions and the related molecular mechanisms during cucumber(Cucumis sativus L.)responses to abiotic stresses,especially salt stress,remain *** previously determined that salt stress induces CsBPC expression in *** this study,Csbpc2 transgene-free cucumber plants were created using a CRISPR/Cas9-mediated editing system to explore CsBPC functions associated with the salt stress *** Csbpc2 mutants had a hypersensitive phenotype,with increased leaf chlorosis,decreased biomass,and increased malondialdehyde and electrolytic leakage levels under salt stress ***,a mutated CsBPC2 resulted in decreased proline and soluble sugar contents and antioxidant enzyme activities,which led to the accumulation of hydrogen peroxide and superoxide ***,the mutation to CsBPC2 inhibited salinity-induced PM-H+-ATPase and V-H+-ATPase activities,resulting in decreased Na+efflux and increased K+*** findings suggest that CsBPC2 may mediate plant salt stress resistance through its effects on osmoregulation,reactive oxygen species scavenging,and ion homeostasis-related regulatory ***,CsBPC2 also affected ABA *** mutation to CsBPC2 adversely affected salt-induced ABA biosynthesis and the expression of ABA signaling-related *** results indicate that CsBPC2 may enhance the cucumber response to salt *** may also function as an important regulator of ABA biosynthesis and signal *** findings will enrich our understanding of the biological functions of BPCs,especially their roles in abiotic stress responses,thereby providing the theoretical basis for improving crop salt tolerance.

关键词： CRISPR/Cas9 stress functions

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Development of a multi-level pH-responsive lipid nanoplatform for efficient co-delivery of si RNA and small-molecule drugs in tumor treatment

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Chinese Chemical Letters 2024年第12期35卷 293-300页

作者： Yunjie Dang Yanru Feng Xiao Chen chaoxing he Shujie Wei Dingyang Liu Jinlong Qi Huaxing Zhang Shaokun Yang Zhiyun Niu Bai Xiang Department of Pharmaceutics Hebei Medical University Department of Pharmacy The Fourth Hospital of Hebei Medical University Core Facilities and Centers Hebei Medical University Department of hematology The Second Hospital of Hebei Medical University

The combination of nucleic acid and small-molecule drugs in tumor treatment holds significant promise;however, the precise delivery and controlled release of drugs within the cytoplasm encounter substantial obstacles, impeding the advancement of formulations. To surmount the challenges associated with precise drug delivery and controlled release, we have developed a multi-level p H-responsive co-loaded drug lipid nanoplatform. This platform first employs cyclic cell-penetrating peptides to exert a multi-level pH response, thereby enhancing the uptake efficiency of tumor cells and endow the nanosystem with effective endosomal/lysosomal escape. Subsequently, small interferring RNA(siRNA) complexes are formed by compacting siRNA with stearic acid octahistidine, which is capable of responding to the lysosome-tocytoplasm pH gradient and facilitate siRNA release. The siRNA complexes and docetaxel are simultaneously encapsulated into liposomes, thereby creating a lipid nanoplatform capable of co-delivering nucleic acid and small-molecule drugs. The efficacy of this platform has been validated through both in vitro and in vivo experiments, affirming its significant potential for practical applications in the co-delivery of nucleic acids and small-molecule drugs.

关键词： Cyclic peptides siRNA Liposomal platform Multi-level pH-responsive Co-delivery

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Dynamic Expression of mi RNAs and Their Targets in the Response to Drought Stress of Grafted Cucumber Seedlings

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Horticultural Plant Journal 2016年第1期2卷 41-49页

作者： LI Chaohan LI Yansu BAI Longqiang he chaoxing YU Xianchang Institute of Vegetables and Flowers Chinese Academy of Agricultural Sciences Horticultural Research Institute Shanghai Academy of Agricultural Sciences

Grafting of cucumber is widely used to improve growth and tolerance to biotic and abiotic stresses. Micro RNAs(miRNAs) regulate plant growth and development, and respond to various stresses through negative, post-transcriptional regulation of the expression of their target genes. We grafted cucumber(Cucumis sativus) scions onto pumpkin(Cucurbita moschata) rootstocks to study the molecular mechanisms of miRNA-mediated graftinginduced responses to drought stress. The relative expressions of 17 selected miRNAs and their predicted target m RNAs were detected by quantitative real-time PCR(q RT-PCR) in leaves and roots of hetero-grafted cucumber seedlings(cucumber as scion and pumpkin as rootstock) and autografted cucumber seedlings(cucumber as scion and rootstock) after 24 hours of 15% PEG6000 treatment. Compared with the expression in leaves of auto-grafted cucumber seedlings, the expressions of most miRNAs in the leaves of hetero-grafted seedlings changed dynamically: induced under normal conditions, and reduced after 3 h of drought stress, and then induced after 8 h and 24 h of drought stress. Similarly, compared with the expression in roots of auto-grafted cucumber seedlings, the expressions of most miRNAs in the roots of hetero-grafted cucumber seedlings changed dynamically: reduced under normal condition, and still reduced after 1 h, but induced after 3 h and 8 h, then reduced significant y after24 h of drought stress. These results are useful for the functional analysis of miRNAs in the mediation of grafting-dependent drought tolerance.

关键词： cucumber graft drought miRNA target

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Effects of Two Kinds of Arbuscular Mycorrhizal Fungi (AMF) Inoculums on Cucumber Seedlings Growth

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Plant Diseases and Pests 2016年第5期7卷 36-38页

作者： Sun Xiuxiu Jin Wenjuan Li Yansu Yan Yan Yu Xianchang he chaoxing Institute of Vegetables and Flowers Chinese Academy of Agricultural Sciences

To study the effects of AMF （ arbuscular mycorrhizal fungi） ineeulums on seedling growth of cucumber, vegetable seeds were inoculated with two kinds of AMF ineculums BF （ mycorrhiza for plants） and VT （ mycorrhiza for vegetables） from Czech Republic during sowing in trays in greenhouse. The root AMF infection rates in seedlings were 49.30% and 37.65% respectively after 35 d growth. The plant height, stem diameter, fresh and dry weight of those seedlings inoculated with BF and VT were higher than that in CK. Compared with the CK, VT inoculation significantly improved the root activity, chlorophyll content and photosynthetic rate of cucumber seedlings. Comprehensive analysis showed that VT could promote seedling growth of protected cucumber.

关键词： Cucumber Arbuscular mycon'hizal fungi (AMF) ineculum Seedling growth Photosynthetic physiology

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生命信息仪处理番茄种子后的生物学效应研究

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种子 2000年第3期19卷 14-15页

作者：贺超兴中国农业科学院蔬菜花卉研究所北京100081

用生命信息仪产生的弱电脉冲处理浸种后的佳粉 1 5番茄种子 ,观察了处理对番茄生长发育的生物学效应。结果表明 :经处理的番茄具有出苗早、出苗齐及出苗率高等特点 ,定植后处理幼苗生长明显优于对照 ,最终处理小区产量坐果数和单果重均... 详细信息

用生命信息仪产生的弱电脉冲处理浸种后的佳粉 1 5番茄种子 ,观察了处理对番茄生长发育的生物学效应。结果表明 :经处理的番茄具有出苗早、出苗齐及出苗率高等特点 ,定植后处理幼苗生长明显优于对照 ,最终处理小区产量坐果数和单果重均增加而产生明显增产效果 ;不同剂量处理结果显示 ,以处理 6 0 min增加最显著 ,45 min次之 ,而处理 75 min产最反而有所降低。

关键词：番茄弱电脉冲生物学效应种子处理生命信息仪

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日光温室甜椒有机土壤栽培试验研究

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农业工程学报 2005年第Z2期21卷 173-177页

作者：张志斌徐知函贺超兴中国农业科学院蔬菜花卉研究所北京100081

利用麦秸与玉米秸、菇渣、草坪草等农产废弃物与土壤有机肥混合配制有机土壤进行了日光温室甜椒栽培越冬生产。结果表明,不同有机化土壤处理均有促进甜椒生长,提高甜椒座果数而增产的效应,有机土壤栽培还可明显提早甜椒成熟期及提高甜... 详细信息

利用麦秸与玉米秸、菇渣、草坪草等农产废弃物与土壤有机肥混合配制有机土壤进行了日光温室甜椒栽培越冬生产。结果表明,不同有机化土壤处理均有促进甜椒生长,提高甜椒座果数而增产的效应,有机土壤栽培还可明显提早甜椒成熟期及提高甜椒产量。

关键词：有机土壤甜椒产量温室

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不同基质接种丛枝菌根真菌对番茄生长及PAL、PPO酶活的影响

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农业工程学报 2005年第Z2期21卷 169-172页

作者：贺忠群贺超兴张志斌邹志荣西北农林科技大学园艺学院杨凌712100 中国农业科学院蔬菜花卉研究所北京100081

以番茄为试材,研究常用的几种基质:大田土,有机土,蛭石和草碳混合物接种丛枝菌根真菌(AM F)中的G lom usm osseae(G.m)对番茄生长及PAL、PP 0酶活性的影响。结果表明:接种G.m对番茄株高和茎粗均有显著的促生效应,G.m在蛭石+草碳处理中... 详细信息

以番茄为试材,研究常用的几种基质:大田土,有机土,蛭石和草碳混合物接种丛枝菌根真菌(AM F)中的G lom usm osseae(G.m)对番茄生长及PAL、PP 0酶活性的影响。结果表明:接种G.m对番茄株高和茎粗均有显著的促生效应,G.m在蛭石+草碳处理中对番茄的促生效果最好,总干物重比对照增加131%,不同基质极显著地影响了G.m侵染和扩展,有机土中G.m的侵染率最高。接种35 d,50 d,65 d PAL,PPO酶活均呈现由高到低的趋势,但不同基质间PAL,PPO酶活随侵染率的不同呈现显著差异。

关键词：丛枝菌基质番茄生长 PAL,PPO

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强化蚕种生产中的化性控制

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蚕学通讯 2001年第3期21卷 16-17,63页

作者：贺朝星四川省广元市蚕种场 628017

滞育是家蚕生活史中的自然现象,由于家蚕的滞育特性不同而形成了其不同的化性,也就是确定了家蚕在自然条件下发生世代数的多少.家蚕的化性形成,既受遗传基因的控制,又受环境条件的影响[1].因此,家蚕的化性可以通过人为控制环境条件而控... 详细信息

滞育是家蚕生活史中的自然现象,由于家蚕的滞育特性不同而形成了其不同的化性,也就是确定了家蚕在自然条件下发生世代数的多少.家蚕的化性形成,既受遗传基因的控制,又受环境条件的影响[1].因此,家蚕的化性可以通过人为控制环境条件而控制其发展方向,使化性向着有利于蚕种生产的方向变化,为生产化性稳定的优质蚕种服务.

关键词：家蚕蚕种育种强化生产化性控制

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埃及蔬菜生产科技考察报告

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中国蔬菜 2008年第6期 5-6页

作者：贺超兴张志斌中国农业科学院蔬菜花卉研究所

埃及可耕地面积有限,水资源匮乏,政府鼓励开垦荒地,以低息长期贷款方式出售给大学毕业生;为发展生态农业,政府采取提高农药价格和推广病虫害综合治理技术相结合的方法,使农药用量下降80%。

关键词：科技考察蔬菜生产埃及综合治理技术农药用量水资源匮乏大学毕业生耕地面积

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