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Imprinting, methylation, and expression characterization of the maize ETHYLENE-INSENSITIVE 2-like gene

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The Crop Journal 2019年第1期7卷 49-57页

作者： Xiupeng Mei Ping Li Lu Wang chaoxian liu Lian Zhou Chunyan Li Yilin Cai Maize Research Institute Key Laboratory of Biotechnology and Crop Quality Improvement Ministry of Agriculture Southwest University

The endosperm plays essential roles in embryogenesis and seed germination and provides abundant resources for human food and industrial products. Identification of genes regulating the development of the endosperm and elucidation of their functions is of great importance for maize genetics and breeding. This study showed that the genespecific imprinted gene, ETHYLENE-INSENSITIVE 2-like(EIN2-like), is maternally expressed in both endosperm and embryo. The maternally expressed pattern was maintained throughout later seed developmental stages. Bisulfite sequencing using DNA obtained from hybrid endosperm tissues showed that the upstream regions of the alleles of EIN2-like were highly methylated at symmetrical sites(CG and CHG). A differentially methylated region in the upstream part of the maternal allele of EIN2-like was identified and found to be hypomethylated. Expression analysis showed that EIN2-like was highly expressed in the maize endosperm as well as at different stages of cell differentiation(8–12 days after pollination) in the hybrid endosperm. These results suggest that the maternally expressed gene EIN2-like may play crucial roles in the regulation of seed development.

关键词： Maize Gene imprinting Methylation EIN2-like

Characterization of the imprinting and expression patterns of ZAG2 in maize endosperm and embryo

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The Crop Journal 2015年第1期3卷 74-79页

作者： chaoxian liu Jiuguang Wang Xiupeng Mei Xiaojing Deng Tingting Yu Xiaoli liu Guoqiang Wang Zhizhai liu Yilin Cai Maize Research Institute Southwest University

ZAG2 has been identified as a maternally expressed imprinted gene in maize *** study revealed that paternally inherited ZAG2 alleles were imprinted in maize endosperm and embryo at 14 days after pollination(DAP), and consistently imprinted in endosperm at 10, 12, 16, 18, 20, 22, 24, 26, and 28 DAP in reciprocal crosses between B73 and Mo17. ZAG2 alleles were also imprinted in reciprocal crosses between Zheng 58 and Chang7-2 and between Huang C and 178. ZAG2 alleles exhibited differential imprinting in hybrids of 178 × Huang C and B73 × Mo17, while in other hybrids ZAG2 alleles exhibited binary imprinting. The tissue-specific expression pattern of ZAG2 showed that ZAG2 was expressed at a high level in immature ears, suggesting that ZAG2 plays important roles in not only kernel but ear development.

关键词： Gene imprinting ZAG2 Expression pattern Endosperm Embryo

Molecular mapping and candidate gene analysis of the semi-dominant gene Vestigial glume1 in maize

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The Crop Journal 2019年第5期7卷 667-676页

作者： chaoxian liu Yuanzeng Zhao Yang Bai Xiaomin Lu Weibin Song Liping Qin Yilin Cai Maize Research Institute Southwest UniversityChongqing 400715China Henan Institute of Science and Technology Xinxiang 453003HenanChina Henan Academy of Agricultural Sciences Zhengzhou 450002HenanChina National Maize Improvement Center of China China Agricultural UniversityBeijing 100193China

The glume is an organ of the maize spikelet and plays important roles in anther and kernel *** glume1(Vg1)is a classic mutant associated with ligule and glume *** we report the phenotypic characterization,fine mapping,and candidate gene analysis of the Vg1 ***1 is a semi-dominant and pleiotropic gene,and also affects plant height,ear height,and tassel ***1 ligule degeneration begins at the first leaf,and the Vg1 tassel and ear can be distinguished from those of wild-type plants when their lengths reach respectively 55 mm and 51 *** a BC3 mapping population of 11,445 plants,we delimited the Vg1 functional site to an interval of 7.4 kb,flanked by the markers InDelLM and CRM6.A putative cyclopropane fatty-acid synthase gene(ZmCPA-FAS1)was hypothesized to underlie the mutant *** detected a Helitron insertion in the sixth intron of *** presence caused abnormal alternative splicing of ZmCPA-FAS1 that conferred new characteristics on the Vg1 *** findings are a basis for further discovery of the molecularmechanism underlying glume development and a potential guide formaize breeding of small-glume varieties,especially sweet corn breeding.

关键词： Vestigial glume1 Fine mapping Candidate gene analysis ZmCPA-FAS1 Glume development

Identification and Fine Mapping of rhm1 Locus for Resistance to Southern Corn Leaf Blight in Maize

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Journal of Integrative Plant Biology 2012年第5期54卷 321-329页

作者： Yuanzeng Zhao Xiaomin Lu chaoxian liu Haiying Guan Mei Zhang Zhongfeng Li Hongwei Cai Jinsheng Lai State Key Laboratory of Agrobiotechnology and National Maize Improvement Center Department of Plant Genetics and BreedingChina Agricultural UniversityBeijing 100193China Department of Life Science and Technology Henan Institute of Science and TechnologyXinxiang 453003China Henan Academy of Agricultural Sciences Zhengzhou 450002China

rhml is a major recessive disease resistance locus for Southern corn leaf blight （SCLB）. To further narrow down its genetic position, F2 population and BCIFI population derived from the cross between resistant （H95rhm） and susceptible parents （H95） of maize （Zea mays） were constructed. Using newly developed markers, rhml was initially delimited within an interval of 2.5 Mb, and then finally mapped to a 8.56 kb interval between InDel marker IDP961-503 and simple sequence repeat （SSR） marker A194149--1. Three polymorphic markers IDP961-504, IDP B2-3 and A194149-2 were shown to be co-segregated with the rhml locus. Sequence analysis of the 8.56 kb DNA fragment revealed that it contained only one putative gene with a predicted amino acid sequence identical to lysine histidine transporter 1 （LHT1）. Comparative sequence analysis indicated that the LHT1 in H95rhrn harbors a 354 bp insertion in its third exon as compared with that of susceptible alleles in B73, H95 and Mo17. The 354 bp insertion resulted in a truncation of the predicted protein of candidate resistance allele （LHT1-H95rhm）. Our results strongly suggest LHTI as the candidate gene for rhml against SCLB. The tightly linked molecular markers developed in this study can be directly used for molecular breeding of resistance to Southern corn leaf blight in maize.

关键词： Bipolaris maydis fine-mapping maize rhml Southern corn leaf blight Zea mays.

Identification and Characterization of ZF-HD Genes in Response to Abscisic Acid and Abiotic Stresses in Maize

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Phyton-International Journal of Experimental Botany 2023年第3期92卷 707-723页

作者： Xiaojie Jing Chunyan Li Chengjuan Luo Chaonan Yao Jiahao Zhang Tingting Zhu Jiuguang Wang chaoxian liu College of Agronomy and Biotechnology Southwest UniversityChongqing400715China Engineering Research Center of South Upland Agriculture Ministry of EducationChongqing400715China Key Laboratory of Application and Safety Control of Genetically Modified Crops Chongqing400715China

The zinc finger homeodomain(ZF-HD)genes belong to the homeobox gene family,playing critical roles in flower development and stress *** their importance,however,to date there has been no genome-wide identification and characterization of the ZF-HD genes that are probably involved in stress responses in *** this study,24 ZF-HD genes were identified,and their chromosomal locations,protein properties,duplication patterns,structures,conserved motifs and expression patterns were *** results revealed that the ZF-HD genes are unevenly distributed on nine chromosomes and that most of these genes lack *** and two ZF-HD genes have undergone segmental and tandem duplication,respectively,during genome *** 24 ZF-HD transcription factors were classified into six major groups on the basis of protein molecular evolutionary *** expression profiles of these genes in different tissues were evaluated,resulting in producing two distinct ***-HD genes are preferentially expressed in reproductive ***,expression profiles of the 24 ZF-HD genes in response to different kinds of stresses revealed that ten genes were simultaneously up-regulated under ABA,salt and PEG treatments;meanwhile four genes were simultaneously *** findings will pave the way for deciphering the function and mechanism of ZF-HD genes on how to implicate in abiotic stress.

关键词： Maize(Zea mays L.) ZF-HD evolutionary relationship expression pattern abiotic stress

多叶排烟口/多叶加压送风口气密性标准如何应用的探讨

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暖通空调 2011年第11期41卷 86-91页

作者：刘朝贤中国建筑西南设计研究院有限公司

针对多叶排烟口/多叶送风口气密性标准只是从宏观角度反映关闭风口气流泄漏的综合性控制指标,无法直接用于加压送风系统关闭风口漏风量计算,提出将风口气密性标准转换成能表征漏风量与压差之间的函数关系的两个替代参数———风口漏风... 详细信息

针对多叶排烟口/多叶送风口气密性标准只是从宏观角度反映关闭风口气流泄漏的综合性控制指标,无法直接用于加压送风系统关闭风口漏风量计算,提出将风口气密性标准转换成能表征漏风量与压差之间的函数关系的两个替代参数———风口漏风面积率和压差指数的方法,建立了两者的计算数学模型。并对国内外部分风口气密性标准的转换进行了数值计算,为关闭风口漏风量计算提供了支持。

关键词：风口气密性标准替代参数漏风面积率压差指数漏风量

利用具有注意力的Mask R-CNN检测震害建筑物立面损毁

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武汉大学学报（信息科学版） 2020年第11期45卷 1660-1668页

作者：眭海刚黄立洪刘超贤武汉大学测绘遥感信息工程国家重点实验室湖北武汉430079

震后建筑物损毁信息是灾情快速评估和应急救援的重要决策依据之一。针对传统的建筑物损毁遥感检测技术只关注于顶面信息,导致众多顶面结构完好而中间层、底层倒塌或崩裂的损毁建筑物处于检测盲点的问题,提出了一种融合引入注意力机制的... 详细信息

震后建筑物损毁信息是灾情快速评估和应急救援的重要决策依据之一。针对传统的建筑物损毁遥感检测技术只关注于顶面信息,导致众多顶面结构完好而中间层、底层倒塌或崩裂的损毁建筑物处于检测盲点的问题,提出了一种融合引入注意力机制的深度学习实例分割模型和图像多尺度分割算法进行震后建筑物立面损毁检测的方法。首先,利用卷积块状注意力模块(convolutional block attention module,CBAM)改进掩模区域卷积神经网络(mask region-based convolutional networks,Mask R-CNN)模型实现了复杂建筑物立面背景中的损毁信息提取;然后,基于建筑物立面影像多尺度分割结果,利用多数投票规则实现了损毁检测结果的后处理优化。实验结果表明,相比传统损毁检测方法,所提方法能够更有效地实现震后建筑物立面损毁信息的精准定位,总体准确率可达到89.15%。

关键词：地震建筑物立面损毁地面影像 Mask R-CNN 注意力机制多尺度分割

对防烟楼梯间及其合用前室分别加压送风防烟方案的流体网络分析

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暖通空调 2011年第1期41卷 64-70页

作者：刘朝贤中国建筑西南设计研究院有限公司

应用流体网络分析法,对现行高层建筑防火规范第8章第8.3.2条表8.3.2-2中的风量控制数据进行了校验性计算,揭示了分别加压送风的流动特性。计算结果表明,向防烟楼梯间加压是徒劳的,因为加压送风会直接从防烟楼梯间的疏散外门流失。

关键词：分别加压疏散外门节点平衡闭合点流量校正

对现行加压送风防烟方案泄压问题的分析与探讨

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暖通空调 2010年第9期40卷 63-73页

作者：刘朝贤中国建筑西南设计研究院有限公司

对现行泄压法以开门时和关门时的风量差(Lv-Ly)作为泄压与选择泄压装置的依据存在的问题作了分析,提出了以推门力作为判定依据;以关门时疏散人员能安全进入正压间为目标,对四种加压送风防烟方案按不同情况分别采取了综合性对策措施,并... 详细信息

对现行泄压法以开门时和关门时的风量差(Lv-Ly)作为泄压与选择泄压装置的依据存在的问题作了分析,提出了以推门力作为判定依据;以关门时疏散人员能安全进入正压间为目标,对四种加压送风防烟方案按不同情况分别采取了综合性对策措施,并对综合性对策措施进行了计算和论证,并得出了结论。

关键词：推门力开启力矩正压力推门力矩自平衡能力防火门左右扇反向