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Allosteric conformational changes of G proteins upon its interaction with membrane and GPCR

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Chinese Chemical Letters 2022年第2期33卷 747-750页

作者： Longmei Li Jin Zhang Wenjing Sun Weimin Gong Changlin Tian Pan shi chaowei shi Hefei National Laboratory of Physical Science at Microscale and School of Life Sciences University of Science and Technology of ChinaHefei 230027China Department of Chemical Physics at School of Chemistry and Materials Sciences University of Science and Technology of ChinaHefei 230027China

Current resolved structures of GPCRs and G protein complexes provided important insights into G protein activation. However, the binding or dissociation of GPCRs with G protein is instantaneous and highly dynamic in the intracellular environment. The conformational dynamic of G protein still needs to be addressed. In this study, we applied ^(19)F solution NMR spectroscopy to monitor the conformational changes of G protein upon interact with detergent mimicking membrane and receptor. Our results show that there are two states equilibria in the G_(α)in apo states. The interaction of G_(α)with detergents will accelerate this conformational transformation and induce a state that tends to bind to GPCRs. Finally, the G_(α)proteins presented a fully activation state when they coupled to GPCRs.

关键词： 19F solution NMR G protein-coupled receptors Conformational dynamics G protein

Effi cient long-distance NMR-PRE and EPR-DEER restraints for two-domain protein structure determination

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Protein & Cell 2013年第12期4卷 893-896页

作者： Kaiqi Wu chaowei shi Juan Li Haipeng Wang Pan shi Liu Chen Fangming Wu Ying Xiong Changlin Tian Hefei National Laboratory for Physical Science at the Microscale&School of Life Science University of Science and Technology of ChinaHefei 230026China High Magnetic Field Laboratory Chinese Academy of SciencesHefei 230031China

Dear Editor,The functional diversity of proteins is related to the cooperation of multiple *** globular domains are typically joined by a fl exible length of polypeptide chain,which makes the structural analysis of multi-domain proteins diffi ***,we describe the combined use of solution NMR(nuclear magnetic resonance)and EPR(elec-tron paramagnetic resonance)for the structural analysis of a protein with two separate *** structure of each domain was determined independently using conventional NMR restraints,and the relative orientation of the two separate domains was confi ned using long-distance restraints obtained by NMR-PRE(paramagnetic relaxation enhancement)and EPR-DEER(double electron-electron resonance,also called PELDOR:pulsed electron double reso-nance.

关键词： paramagnetic structure double

A Real-time Lithological Identification Method based on SMOTE-Tomek and ICSA Optimization

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Acta Geologica Sinica(English Edition) 2024年第2期98卷 518-530页

作者： DENG Song PAN Haoyu LI chaowei YAN Xiaopeng WANG Jiangshuai shi Lin PEI Chunyu CAI Meng College of Petroleum Engineering Changzhou UniversityChangzhouJiangsu 213000China

In petroleum engineering,real-time lithology identification is very important for reservoir evaluation,drilling decisions and petroleum geological exploration.A lithology identification method while drilling based on machine learning and mud logging data is studied in this *** method can effectively utilize downhole parameters collected in real-time during drilling,to identify lithology in real-time and provide a reference for optimization of drilling *** the imbalance of lithology samples,the synthetic minority over-sampling technique(SMOTE)and Tomek link were used to balance the sample number of five ***,this paper introduces Tent map,random opposition-based learning and dynamic perceived probability to the original crow search algorithm(CSA),and establishes an improved crow search algorithm(ICSA).In this paper,ICSA is used to optimize the hyperparameter combination of random forest(RF),extremely random trees(ET),extreme gradient boosting(XGB),and light gradient boosting machine(LGBM)*** addition,this study combines the recognition advantages of the four *** accuracy of lithology identification by the weighted average probability model reaches *** study of this paper realizes high-precision real-time lithology identification method,which can provide lithology reference for the drilling process.

关键词： mud logging data real-time lithological identification improved crow search algorithm petroleum geological exploration SMOTE-Tomek

Sun Smart Tracking Methods Based on Image Processing

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Sun Smart Tracking Methods Based on Image Processing

Structural Basis of C-terminalβ-catenin Recognition by Tax-interacting Protein-1

2011 2nd Asia-Pacific Conference on Wearable Computing Systems

作者： Cui Yan shi chaowei Chen Jingjing Qi Shuguang Wang Lidong College of Science Tianjin Polytechnic university

By using one of image processing technology direct to sun smart tracking methods, we can properly rotate solar energy collecting devices such as solar water heaters and solar panels, making them straight to sun to improve the efficiency of collecting solar energy. In the process of image processing, we use hough transform to abstract straight lines and intersection points of images, through which we calculate direction angle and elevation. We can collect solar energy effectively by rotating solar collecting devices to proper positions driven by drive motors. This application has an important significance to make good use of solar energy and environmental protection of China even the whole world.

关键词： image processing solar energy Matlab spotlight hough transform

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Structural Basis of C-terminalβ-catenin Recognition by Tax-...

中国晶体学会第四届全国会员代表大会暨学术会议

作者： Jinxiu Zhang Xiaojie Yan chaowei shi Xue Yang Yu Guo Changlin Tian Jiafu Long Yuequan Shen Tianjin Key Laboratory of Protein Science College of Life ScienceNanKai University College of Life Science University of Science and Technology China

＜正＞β-catenin is a key component of the canonical Wnt signaling ***-interacting protein-1（TIP-1） is an atypical scaffolding protein containing one PDZ domain only and able to bind c-terminalβ-catenin with high affinity and thus inhibit its transcriptional *** resolution crystal structures of c-terminalβ-catenin bound TIP-1 complex,TIP-1 wild type and c-terminal truncated TIP-1 have been *** comparison of peptide-free and peptide-bound TIP-1 reveals that drastic conformational changes of loopβB-βC region are required to avoid heavy conflict with incoming c-terminalβ-catenin,which is in sharp contrast with other published structures of PDZ domains where free form conformation is ready to take *** addition to the last four amino acids ofβ-catenin bound to canonical peptide binding pocket of PDZ domain,loopβB-βC region of TIP-1 forms an additional binding cavity to anchor more amino acids through a hydrophobic residue pair of Trp776 and *** presumably is the cause of much higher affinity of c-terminalβ-catenin binding to TIP-1 than other PDZ *** of a conserved amino acid Pro45 to alanine or serine and Trp776 to alanine leads to about 15-fold and 80-fold decreased binding affinity measured by isothermal titration calorimetry experiments,*** results provide first molecular details of c-terminalβ-catenin recognition by TIP-1 protein.

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论我国驰名商标的法律保护

临沧教育学院学报 2006年第4期15卷 57-59页

作者：史朝伟云南大学法学院云南昆明650091

随着我国社会主义市场经济体制的确立,国内外交往日益增多。在激烈的市场竞争中,以创立、发展和保护驰名商标为主要内容的战略工程已成为提高我国企业商品竞争力的重要方法之一。为此,通过分析我国驰名商标保护的现状与不足,为我国驰名... 详细信息

随着我国社会主义市场经济体制的确立,国内外交往日益增多。在激烈的市场竞争中,以创立、发展和保护驰名商标为主要内容的战略工程已成为提高我国企业商品竞争力的重要方法之一。为此,通过分析我国驰名商标保护的现状与不足,为我国驰名商标的保护提出一些立法建议。

关键词：驰名商标法律保护立法建议

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ZGA121型整经机的优化与改进设计

山东纺织科技 2011年第3期52卷 42-43页

作者：娄建锋石超伟恒天重工股份有限公司河南郑州450001

文章从计长装置、伸缩箱浮动装置、经轴制动装置、车头墙板方面介绍了ZGA121型整经机的优化与改进设计。

关键词： ZGA121整经机优化设计改进

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基于混合流量预测的虚拟网络拓扑重构方法

系统工程与电子技术 2021年第5期43卷 1382-1388页

作者：史朝卫孟相如康巧燕苏玉泽空军工程大学信息与导航学院陕西西安710077 中国人民解放军94303部队山东潍坊261000

目前在构建虚拟网络时,为满足用户动态变化的带宽需求,虚拟网络控制平台通常把虚拟链路带宽设置为流量最大值,一定程度上造成了资源浪费。针对这一问题,提出一种基于混合流量预测的虚拟网络拓扑重构方法,利用基于参数优化选择的混合流... 详细信息

目前在构建虚拟网络时,为满足用户动态变化的带宽需求,虚拟网络控制平台通常把虚拟链路带宽设置为流量最大值,一定程度上造成了资源浪费。针对这一问题,提出一种基于混合流量预测的虚拟网络拓扑重构方法,利用基于参数优化选择的混合流量预测算法对下一周期的网络流量进行预测,根据流量预测结果进行拓扑重构,在避免出现乒乓效应的同时节省更多带宽资源。为了提高流量预测算法的精度与效率,首先采用小波分解方法将流量数据分解为高频的细节时间序列和低频的近似时间序列,然后利用基于粒子群优化的相空间重构方法,对该时间序列进行特征提取构建训练样本。之后分别采用混沌模型对细节时间序列进行训练预测,采用极限学习机(extreme learning machine,ELM)神经网络对近似时间序列进行训练预测。仿真结果表明,所提的流量预测算法在保证预测精度的同时,运行时间更短,预测效率更高,进而保证了拓扑重构方法可以节省更多的带宽资源。

关键词：流量预测拓扑重构小波分解相空间重构极限学习机

桂东南岑溪—陆川地区斜长角闪岩的年代学、地球化学特征及其地质意义

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高校地质学报 2024年第2期30卷 178-195页

作者：梁任鑫时毓庞超伟刘希军桂林理工大学广西隐伏金属矿产勘查重点实验室桂林541004 桂林理工大学有色金属矿产勘查与资源高效利用协同创新中心桂林541004

文章对桂东南岑溪—陆川地区的斜长角闪岩进行了锆石U-Pb年代学和全岩地球化学分析,研究结果表明,研究区斜长角闪岩的形成时代不早于早古生代(487~428 Ma)。中—新太古代的古老锆石揭示该地区深部可能存在未暴露的太古代结晶基底。全岩... 详细信息

文章对桂东南岑溪—陆川地区的斜长角闪岩进行了锆石U-Pb年代学和全岩地球化学分析,研究结果表明,研究区斜长角闪岩的形成时代不早于早古生代(487~428 Ma)。中—新太古代的古老锆石揭示该地区深部可能存在未暴露的太古代结晶基底。全岩地球化学分析显示,样品相对于原始地幔富集大离子亲石元素(LILE)如K、Rb、Ba、Sr等和轻稀土元素,明显亏损高场强元素(HFSE)如Nb、Ta、Ti等,类似于岛弧玄武岩(IAB)的特征。研究区斜长角闪岩由古老俯冲交代的地幔楔熔融而成,可能形成于早古生代碰撞后的岩石圈伸展阶段。

关键词：早古生代构造事件斜长角闪岩锆石年代学地球化学岩石学桂东南