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Differential DNA methylation profiles of human B lymphocytes and Epstein-Barr virus-immortalized B lymphocytes

Chinese Journal of Cancer Research 2018年第1期30卷 104-111页

作者： chaoting zhang Rui zhang Zhiqiang Chen Jing Chen Jigang Ruan Zheming Lu Hongchao Xiong Wenjun Yang Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education/Beijing) Laboratory of Biochemistry and Molecular BiologyPeking University Cancer Hospital & Institute Beijing 100142 China Key Laboratory of Fertility Preservation and Maintenance the School of Basic Medicine the General Hospital Ningxia Medical University Yinchuan 750004 China Department of Radiology the General HospitalNingxia Medical University Yinchuan 750004 China Department of Digestive System the General Hospital Ningxia Medical UniversityYinchuan 750004 China Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education/Beijing) Department of Thoracic Surgery Peking University Cancer Hospital & Institute Beijing 100142 China

Objective： This study aimed to comprehensively assess Epstein-Barr virus（EBV）-induced methylation alterations of B cell across whole ***： We compared DNA methylation patterns of primary B cells and corresponding lymphoblastoid cell lines（LCLs） from eight participants. The genome-wide DNA methylation profiles were compared at over 850,000 genome-wide methylation ***： DNA methylation analysis revealed 87,732 differentially methylated Cp G sites, representing approximately 12.41% of all sites in LCLs compared to primary B cells. The hypermethylated and hypomethylated Cp G sites were about 22.75% or 77.25%, respectively. Only 0.8% of hypomethylated sites and 4.5% of hypermethylated sites were located in Cp G islands, whereas 8.0% of hypomethylated sites and 16.3% of hypermethylated sites were located in shore（N_shore and S_shore）. Using principal component analysis of the DNA methylation profiles, primary B cells and LCLs could be accurately predicted. Gene Ontology（GO） and Kyoto Encyclopedia of Genes and Genomes（KEGG） analysis of differently methylated genes revealed that most of the top GO biological processes were related to cell activation and immune response, and some top enrichment pathways were related with activation and malignant transformation of human B ***： Our study demonstrated genome-wide DNA methylation variations between primary B cells and corresponding LCLs, which might yield new insight on the methylation mechanism of EBV-induced immortalization.

关键词： DNA methylation Epstein-Barr virus next generation sequencing B cells LCLs

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Oral microbiome and risk of malignant esophageal lesions in a high-risk area of China:A nested case-control study

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Chinese Journal of Cancer Research 2020年第6期32卷 742-754页

作者： Fangfang Liu Mengfei Liu Ying Liu Chuanhai Guo Yunlai Zhou Fenglei Li Ruiping Xu Zhen Liu Qiuju Deng Xiang Li chaoting zhang Yaqi Pan Tao Ning Xiao Dong Zhe Hu Huanyu Bao Hong Cai Isabel Dos Santos Silva Zhonghu He Yang Ke Key Laboratory of Carcinogenesis and Translational Research(Ministry of Education/Beijing) Laboratory of GeneticsPeking University Cancer Hospital&InstituteBeijing 100142China Novogene Co. LtdBeijing 100080China Hua County People's Hospital Anyang 456400China Anyang Cancer Hospital Anyang 455000China Department of Non-communicable Disease Epidemiology London School of Hygiene&Tropical MedicineLondon WC1E 7HTUK

Objective:We aimed to prospectively evaluate the association of oral microbiome with malignant esophageal lesions and its predictive potential as a biomarker of ***:We conducted a case-control study nested within a population-based cohort with up to 8 visits of oral swab collection for each subject over an 11-year period in a high-risk area for esophageal cancer in *** oral microbiome was evaluated with 16 S ribosomal RNA(rRNA)gene sequencing in 428 pre-diagnostic oral specimens from 84 cases with esophageal lesions of severe squamous dysplasia and above(SDA)and 168 matched healthy *** analysis was performed to identify taxa of differential *** oral species together with subject characteristics were evaluated for their potential in predicting SDA risk by constructing conditional logistic regression ***:A total of 125 taxa including 37 named species showed significantly different abundance between SDA cases and controls(all P***:The oral microbiome may play an etiological and predictive role in esophageal cancer,and it holds promise as a non-invasive early warning biomarker for risk stratification for esophageal cancer screening programs.

关键词： Early warning biomarker esophageal squamous cell carcinoma oral microbiome risk prediction

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个体化肿瘤特异TCR的筛选策略

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中国肿瘤生物治疗杂志 2021年第9期28卷 863-868页

作者：张超亭陆哲明北京大学肿瘤医院暨北京市肿瘤防治研究所生化与分子生物学研究室恶性肿瘤发病机制及转化研究教育部重点实验室北京100142

基因修饰T细胞疗法在肿瘤治疗领域取得突破性进展,主要包括嵌合抗原受体基因修饰T(chimeric antigen receptor engineered T,CAR-T)细胞和T细胞受体基因修饰T(T-cell receptor modified T,TCR-T)细胞。虽然CAR-T细胞疗法在血液系统肿瘤... 详细信息

基因修饰T细胞疗法在肿瘤治疗领域取得突破性进展,主要包括嵌合抗原受体基因修饰T(chimeric antigen receptor engineered T,CAR-T)细胞和T细胞受体基因修饰T(T-cell receptor modified T,TCR-T)细胞。虽然CAR-T细胞疗法在血液系统肿瘤治疗领域呈现良好的临床治疗效果,但CAR-T细胞仅能识别肿瘤细胞膜抗原(占细胞全部抗原的比例约10%),而TCR-T细胞可以识别人白细胞抗原(human leukocyte antigen,HLA)提呈的细胞内抗原,因此TCR-T细胞可以识别更多种类的肿瘤抗原,进而实现对CAR-T细胞的合理补充。由于TCR-T细胞需要同时识别细胞内抗原和对应的HLA,而不同患者的HLA分型和表达的肿瘤抗原都可能存在巨大差异,因此有必要为每个/每类肿瘤患者定制个体化的TCR-T细胞,其中的关键为筛选特异识别肿瘤抗原的TCR。当前主要有筛选靶向“已知”肿瘤抗原TCR和筛选靶向“未知”肿瘤抗原TCR的两种策略,但其各有适用性,应针对每个患者制定适合的筛选方法,以制备多种肿瘤特异性TCR-T细胞,从而实现个体化TCR-T细胞的肿瘤治疗。

关键词：肿瘤 T细胞受体 T细胞受体基因修饰T细胞疗法

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Hydrophilic bismuth sulfur nanoflower superstructures with an improved photothermal efficiency for ablation of cancer cells

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Nano Research 2016年第7期9卷 1934-1947页

作者： Zhiyin Xiao chaoting Xu Xiaohong Jiang Wenlong zhang Yuxuan Peng Rujia Zou Xiaojuan Huang Qian Liu Zongyi Qin Junqing Hu State Key Laboratory for Modification of Chemical Fibers and Polymer Materials College of Materials Science and Engineering Donghua University Shanghai 201620 China 2 College of Biological Chemical Sciences and Engineering Jiaxing University Jiaxing 314001 China School of Medicine Science Jiaxing University Jiaxing 314001 China College of Science Donghua University Shanghai 201620 China

Nanomaterials with intense near-infrared （NIR） absorption exhibit effective photon-to-thermal energy transfer capabilities and can generate heat to ablate cancer cells, thus playing a pivotal role in photothermal cancer therapeutics. Herein, hydrophilic flower-like bismuth sulfur （Bi2S3） superstructures with uniform size and improved NIR absorption were controllably synthesized via a facile solvothermal procedure assisted by polyvinylpyrrolidone （PVP）, which could adjust the product morphology. Induced by an 808-nm laser, the as-prepared Bi2S3 nanoflowers exhibited much higher photothermal conversion efficiency （64.3%） than that of Bi2S3 nanobelts （36.5%） prepared in the absence of PVP. This can be attributed not only to the Bi2S3 nanoflower superstructures assembled by 3-dimensional crumpled-paper-like nanosheets serving as many laser-cavity mirrors with improved reflectivity and absorption of NIR light but also to the amorphous structures with a lower band gap. Thus, to achieve the same temperature increase, the concentration or laser power density could be greatly reduced when using Bi2S3 nanoflowers compared to when using Bi2S3 nanobelts, which makes them more favorable for use in therapy due to decreased toxicity. Furthermore, these Bi2S3 nanoflowers effectively achieved photothermal ablation of cancer ceils in vitro and in vivo. These results not only supported the Bi2S3 nanoflowers as a promising photothermal agent for cancer therapy but also paved an approach to exploit new agents with improved photothermal efficiency.

关键词： bismuth sulfur nanoflowers photothermal agent photothermal conversionefficiency cancer therapy

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Unimodal productivity-biodiversity relationship along the gradient of multidimensional resources across Chinese grasslands

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National Science Review 2022年第12期9卷 90-103页

作者： Yanfen Wang Jianqing Du Zhe Pang Yali Liu Kai Xue Yann Hautier Biao zhang Li Tang Lili Jiang Baoming Ji Xingliang Xu Jing zhang Ronghai Hu Shutong Zhou Fang Wang Rongxiao Che Di Wang chaoting Zhou Xiaoyong Cui Nico Eisenhauer Yanbin Hao College of Resources and Environment University of Chinese Academy of Sciences State Key Laboratory of Tibetan Plateau Earth System Environment and Resources Chinese Academy of Sciences Beijing Yanshan Earth Critical Zone National Research Station University of Chinese Academy of Sciences School of Grassland Science Beijing Forestry University Ecology and Biodiversity Group Department of BiologyUtrecht University Key Laboratory of Ecosystem Network Observation and Modeling Institute of Geographic Sciences and Natural Resources Research Chinese Academy of Sciences College of Life Sciences University of Chinese Academy of Sciences School of Ecology and Environmental Sciences Yunnan University Institute of Biophysics Chinese Academy of Sciences Department of Medicine New York University Grossman School of Medicine Beijing Yanshan Earth Critical Zone National Research Station University of Chinese Academy of Sciences College of Life Sciences University of Chinese Academy of Sciences German Centre for Integrative Biodiversity Research (iDiv) Institute of Biology Leipzig University

Resources can affect plant productivity and biodiversity simultaneously and thus are key drivers of their relationships in addition to plant-plant ***,most previous studies only focused on a single resource while negle cting the nature of resource *** we integrated four essential resources for plant growth into a single metric of resource diversity(RD) to investigate its effects on the productivity-biodiversity relationship(PBR) across Chinese *** showed that habitats differing in RD have different PBRs—positive in low-resource habitats,but neutral in medium-and high-resource ones—while collectively,a weak positive PBR was observe ***,when excluding direct effects of RD on productivity and biodiversity,the PBR in high-resource habitats became negative,which leads to a unimodal instead of a positive PBR along the RD *** integrating resource effects and changing plant-plant interactions into a unified framework with the RD gradient,our work contributes to uncovering underlying me chanisms for inconsistent PBRs at large scales.

关键词： productivity–biodiversity relationship resource diversity plant strategies grassland

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Development of Wireless Light Control System Based on Zigbee

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Communications and Network 2013年第1期5卷 29-33页

作者： Changfei Guo Xiaoping Zou chaoting Ma Rongrong zhang Beijing Key Laboratory for Sensor Beijing Information Science and Technology University Beijing China

This paper presents a solution to wireless light control system based on Zigbee. Overall design framework of the system was introduced and analyzed in detail, which contained realization of hardware design. The Zigbee wireless light control system was established based on CC2430-chip of Texas Instruments (TI). In this paper, we achieved designing Printed Circuit Board (PCB) for sensor nodes as end device and coordinator in Protel DXP 2004 and also wrote C codes in IAR embedded workbench development tool to form wireless network. Test results of system show that lights on end device or router in Zigbee wireless sensor networks (WSN) can be controlled by another switch on coordinator, which achieved remotely wireless intelligence light control.

关键词： Wireless Light Control Zigbee PCB WSN

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从分子水平探讨中国东北狍的分类地位

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兽类学报 2005年第1期25卷 14-19页

作者：张明海肖朝庭 Koh Hungsun 东北林业大学野生动物资源学院哈尔滨150040 College of Nature Science

国内外对中国狍的分类地位意见不一。本文测定了来自中国东北完达山、小兴安岭、大兴安岭、三江和吉林向海等 5个地区的 15只野生狍的线粒体DNACytb基因 (4 15bp)和来自完达山、三江和吉林向海等 3个地区的 6只野生狍的线粒体控制区部... 详细信息

国内外对中国狍的分类地位意见不一。本文测定了来自中国东北完达山、小兴安岭、大兴安岭、三江和吉林向海等 5个地区的 15只野生狍的线粒体DNACytb基因 (4 15bp)和来自完达山、三江和吉林向海等 3个地区的 6只野生狍的线粒体控制区部分序列 (76 4bp) ,分析了中国东北狍与西伯利亚狍、欧洲狍的系统进化关系 ,得出中国东北狍与欧洲狍之间mtDNACytb和mtDNA控制区序列差异平均值分别为 4 12 %和 4 89% ,达到了鹿类动物的种间差异水平 ;而与西伯利亚狍的mtDNACytb和mtDNA控制区序列差异平均值仅为 1 6 1%和 2 4 4 % ,只达到亚种水平。结合形态学的差异 ,本文认为中国东北的狍与欧洲狍为两个独立的物种 ,与西伯利亚狍为同种。并提出中国东北狍与欧洲狍的分歧时间约在 2 0 0万年前 ,与西伯利亚狍的分歧时间约在

关键词：分类地位 DNA 控制区序列差异系统进化关系中国东北物种分子水平线粒体形态学

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胰岛素针头使用次数与皮下脂肪增生发生关系的网状Meta分析

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中国全科医学 2020年第34期23卷 4342-4348页

作者：张伟朱冬梅费朝廷周明江苏大学医学院江苏省镇江市212013 江苏省镇江市第一人民医院新区分院 212000

背景皮下脂肪增生是糖尿病患者注射胰岛素的常见并发症。由于“重复使用”的概念较为宽泛,目前研究对重复使用针头是否与脂肪增生发生有关的结论并不一致。目的评价胰岛素针头使用次数与发生皮下脂肪增生的关系。方法于2018年3—11月,检... 详细信息

背景皮下脂肪增生是糖尿病患者注射胰岛素的常见并发症。由于“重复使用”的概念较为宽泛,目前研究对重复使用针头是否与脂肪增生发生有关的结论并不一致。目的评价胰岛素针头使用次数与发生皮下脂肪增生的关系。方法于2018年3—11月,检索PubMed、循证医学数据库(Cochrane Library)、荷兰医学文摘(EMBase)、Science Direct、中国生物文献数据库(CMB)、中国知网(CNKI)等主要数据库,收集胰岛素注射针头使用不同频次(1、2~3、4、5、6~10次)与脂肪增生发生相关的流行病学和实验性研究,并对纳入的研究进行质量评价和数据的提取,检索均从建库至2018年10月,采用Stata 15.0进行网状Meta分析。结果不重复使用针头组脂肪增生的发生概率最低(10.1%);重复使用针头2~10次组间脂肪增生发生情况比较,差异无统计学意义(P>0.05);与不重复使用针头组相比,重复使用针头6~10次组患者脂肪增生发生率明显上升〔OR=3.72,95%CI(1.05,13.17),P0.05)。结论优先推荐一次性使用胰岛素针头,如因条件限制不能做到一针一换,针头使用次数不应超过5次。

关键词：糖尿病胰岛素针头重复使用脂肪增生

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巯基烯烃点击反应制备超疏水聚偏氟乙烯中空纤维膜及其加湿性能

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高分子材料科学与工程 2021年第10期37卷 167-173页

作者：张文欣陈华艳吴朝廷贾悦武春瑞吕晓龙天津工业大学省部共建分离膜与膜过程国家重点实验室天津300387

为更好地发挥中空纤维膜加湿器的加湿作用,提高加湿性能,提出对中空纤维膜进行超疏水改性。利用溶胶凝胶法制备二氧化硅(SiO_(2)),八乙烯基笼型倍半硅氧烷(OV-POSS)与巯基-聚二甲基硅氧烷(PDMS-SH)通过巯基烯烃点击反应形成POSS-S-PDMS... 详细信息

为更好地发挥中空纤维膜加湿器的加湿作用,提高加湿性能,提出对中空纤维膜进行超疏水改性。利用溶胶凝胶法制备二氧化硅(SiO_(2)),八乙烯基笼型倍半硅氧烷(OV-POSS)与巯基-聚二甲基硅氧烷(PDMS-SH)通过巯基烯烃点击反应形成POSS-S-PDMS聚合物,利用涂覆法将其涂覆在聚偏氟乙烯(PVDF)中空纤维膜表面。在膜表面与涂层、涂层与涂层之间均利用化学键连接以提高涂层的牢固度,制备了一种具有SiO_(2)-PDMS-POSS稳定性涂层的超疏水复合膜。研究了光引发剂的质量分数、二氧化硅粒径影响因素对复合膜的影响,并对复合膜进行了表征。最终制备的复合膜接触角达到156°,加湿效率由48%提高为58.32%。

关键词：聚偏氟乙烯中空纤维膜加湿超疏水表面巯基烯烃点击反应二氧化硅

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新型锕填充硼碳笼型化合物的超导电性

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高压物理学报 2024年第2期38卷 78-86页

作者：张王颖刘朝厅陈蕊蒋成澳李培芳闫岩长春大学理学院吉林长春130022 内蒙古民族大学数理学院内蒙古通辽028043

Ac元素作为锕系第一号元素,AcH_(10)的超导转变温度(T_(c))达到251 K,是潜在的室温超导体。XB_(3)C_(3)(X表示不同的金属掺杂元素)是新发现的sp~3笼型化合物,同时具有强共价特性和超导特性,是潜在的高温超导材料。采用第一性原理密度泛... 详细信息

Ac元素作为锕系第一号元素,AcH_(10)的超导转变温度(T_(c))达到251 K,是潜在的室温超导体。XB_(3)C_(3)(X表示不同的金属掺杂元素)是新发现的sp~3笼型化合物,同时具有强共价特性和超导特性,是潜在的高温超导材料。采用第一性原理密度泛函理论,探索以XB_(3)C_(3)、XB_(2)C_(4)和XB_(4)C_(2)笼型结构为原型、引入Ac元素掺杂的AcB_(3)C_(3)、AcB_(2)C_(4)和AcB_(4)C_(2)的晶体结构、晶格动力学、电子性质和超导特性。研究发现:AcB_(2)C_(4)在0~200 GPa区间内难以合成;常压下AcB_(3)C_(3)表现为间接带隙半导体,带隙宽度约为1.154 eV。根据力学稳定性判据可知,AcB_(3)C_(3)和AcB_(4)C_(2)是弹性稳定的具有较高硬度和刚度的脆性材料。同时,常压下AcB_(4)C_(2)表现出超导特性,超导转变温度达到1.565 K。随着压强的增加,超导转变温度呈现先降低后升高的变化趋势,其超导机制由中频声子主导转变为低频与中频声子的共同作用。研究结果可为实验合成笼型化合物超导材料提供理论指导,为探索具有高超导转变温度的超导材料提供新思路。

关键词：笼型化合物第一性原理超导电性超导转变温度

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