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CHD3 chromatin-remodeling factor PICKLE regulates floral transition partially via modulating LEAFY expression at the chromatin level in Arabidopsis

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Science China(life Sciences) 2016年第5期59卷 516-528页

作者： Xing Fu chaonan li Qing liang Yangyang Zhou Hang He liu-Min Fan State Key Laboratory of Protein and Plant Gene Research School of Life Sciences Peking University Beijing 100871 China

PICKLE(PKL), a putative CHD3 chromatin remodeling factor, has been suggested to be involved in multiple processes in Arabidopsis. Here, we confirmed the late-flowering phenotype caused by pkl mutation with pkl mutants in two different ecotypes, and investigated the possible mechanisms that account for PKL regulation of flowering time. Quantitative RT-PCR and RNA-seq assays showed that expression of the LEAFY gene(LFY) and a number of LFY-regulated floral homeotic genes were down-regulated in seedlings of the pkl mutants. As predicted, overexpression of LFY restored normal flowering time of pkl mutants. Our results suggest that PKL may be involved in regulating flowering time via LFY expression. To uncover the underlying mechanism, Ch IP-PCR using anti-PKL was performed on materials from three developmental stages of seedlings. Our results showed that PKL associated with the genomic sequences of LFY, particularly at 10-day and 25-day after germination. We also showed that loss of PKL affected H3K27me3 level at the promoter of LFY. Taken together, our data suggest that transcriptional regulation of LFY at the chromatin level by PKL may at least partially account for the late-flowering phenotype of pkl mutants.

关键词： chromatin-remodeling factor PICKLE floral transition LEAFY Arabidopsis

The protective effects of a D-tetra-peptide hydrogel adjuvant vaccine against H7N9 influenza virus in mice

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Chinese Chemical Letters 2023年第1期34卷 211-214页

作者： Keda Chen Xiaoxin Wu Qingjing Wang Ying Wang Haiyan Zhang Shuai Zhao chaonan li Zhiwen Hu Zhimou Yang Lanjuan li Shulan International Medical College Zhejiang Shuren UniversityHangzhou 310015China State Key Laboratory for Diagnosis and Treatment of Infectious Diseases Collaborative Innovation Center for Diagnosis and Treatment of Infectious DiseasesNational Clinical Research Center for Infectious DiseasesThe First Affiliated HospitalZhejiang University School of MedicineHangzhou 310003China Zhejiang Shuren College Zhejiang Chinese Medical UniversityHangzhou 310053China State Key Laboratory of Medicinal Chemical Biology Key Laboratory of Bioactive MaterialsMinistry of EducationCollege of Life SciencesSynergetic Innovation Center of Chemical Science and Engineeringand National Institute of Functional MaterialsNankai UniversityTianjin 300071China Jiangsu Center for the Collaboration and Innovation of Cancer Biotherapy Cancer InstituteXuzhou Medical UniversityXuzhou 221004China

Repeated waves of influenza virus H7N9 epidemics after 2013 have caused severe influenza in humans,with mortality reaching approximately 40%–50%.To prevent possible pandemics,the development of highly effective vaccines against influenza virus H7N9 is highly *** the present study,by taking advantage of the D-tetra-peptide adjuvant(G^(D)F^(D)F^(D)Y),we reported a simple method to prepare H7N9 ***(Npx),with good anti inflammatory and broad anti-viral effects,was employed as an N-terminal capping group to construct a hydrogel precursor,Npx-G^(D)F^(D)F^(D)*** hydrogel adjuvant was prepared using a routine heating cooling protocol and the final vaccine was ready after mixing with the split A/Zhejiang/DTID-ZJU01/2013(H7N9)antigen by *** with the traditional Al(OH)_(3) adjuvant vaccine and the split vaccine,our hydrogel adjuvant vaccine showed the best preventive effects against H7N9 infection.A mechanistic study illustrated that higher antibody responses and variations in cytokine expression might account for its increased protective *** strategy demonstrated the advantages of a peptide hydrogel adjuvant in the application of vaccines against H7N9 and demonstrated its potential application in vaccines against emerging threats from other viruses.

关键词： H7N9 influenza virus Hydrogel Adjuvant Vaccine Preventative effects

Community assemblage of free-living diazotrophs along the elevational gradient of Mount Gongga

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Soil Ecology Letters 2019年第3期1卷 136-146页

作者： Yansu Wang chaonan li Zehao Shen Junpeng Rui Decai Jin Jiabao li Xiangzhen li Key Laboratory of Biotechnology for Medicinal Plants of Jiangsu Province and School of life Sciences Jiangsu Normal UniversityXuzhou 221116China Key Laboratory of Environmental and Applied Microbiology CAS/Environmental Microbiology Key Laboratory of Sichuan ProvinceChengdu Institute of BiologyChinese Academy of SciencesChengdu 610041China Department of Ecology Ministry of Education Key Laboratory for Earth Surface ProcessesPeking UniversityBeijing 100871China Key Laboratory of Environmental Biotechnology Research Center for Eco-Environmental SciencesChinese Academy of SciencesBeijing 100085China

Mountain systems are unique for studying the responses of species distribution and diversity to environmental changes along elevational *** is well known that free-living diazotrophic microorganisms are important to nitrogen cycling in mountain ***,the elevational patterns of free-living diazotrophs and the underlying ecological processes in controlling their turnover along broader gradients are less well ***,we investigated the pattern of diazotrophic diversity along the elevational gradient(1800 m-4100 m)in Mount Gongga of *** results showed that the α-diversity of diazotrophs did not change with the elevation from 1800 m to 2800 m,but decreased at elevations above 3000 *** diversity pattern was driven mainly by soil total carbon,nitrogen,and plant *** diazotrophic taxa showed differential abundance-elevation *** processes determining diazotrophic community assemblage shift along the *** processes were relatively stronger at both low and high elevations,whereas stochastic processes were stronger at the middle *** study also suggested a strong relationship among aboveground plants and diazotrophs,highlighting their potential interactions,even for free-living diazotrophs.

关键词： Diazotrophs Community assemblage Elevational gradient Mount Gongga

Soil total organic carbon/total nitrogen ratio as a key driver deterministically shapes diazotrophic community assemblages during the succession of biological soil crusts

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Soil Ecology Letters 2021年第4期3卷 328-341页

作者： lin Xu Bingchang Zhang Entao Wang Bingjian Zhu Minjie Yao chaonan li Xiangzhen li Engineering Research Center of Soil Remediation of Fujian Province University College of Resources and EnvironmentFujian Agriculture and Forestry UniversityFuzhou 350002China Geographical Science College Shanxi Normal UniversityLinfen 041004China Escuela Nacional de Ciencias Biológicas Instituto Politécnico NacionalCD.México 11340México Key Laboratory of Environmental and Applied Microbiology CASEnvironmental Microbiology Key Laboratory of Sichuan ProvinceChengdu Institute of BiologyChinese Academy of SciencesChengdu 610041China

The diazotrophic community in biological soil crusts(biocrusts)is the key supplier of nitrogen in *** date,there is still limited information on how biocrust development influences the succession of diazotrophic community,and what are the most important factors mediating diazotrophic communities during biocrust *** the high throughput nifH amplicon sequencing,the diazotrophs in soils at different developmental stages of biocrust were comparatively *** results evidenced the decreases of TOC/TN ratio and pH value with biocrust *** and Scytonema were the most dominant diazotrophic genera at all biocrust stages,while Azospirillum and Bradyrhizobium were abundant only in bare *** co-occurrence networks tended to be less complex and less connected with biocrust *** soil TOC/TN ratio was the most dominant factor mediating diazotrophic diversity,community composition and assembly processes,while diazotrophic-diversity and NO3–-N/NH4+-N ratio were positively correlated with the nitrogenase activity during biocrust *** study provided novel understandings of nitrogen fixation and succession patterns of diazotrophic community,by showing the effects of biocrust succession on diazotrophic diversity,community composition,community assembly and co-occurrence networks,and recognizing TOC/TN ratio as the most dominant factor mediating diazotrophs during biocrust succession.

关键词： Biological soil crust succession nifH amplicon sequencing Diazotrophs Community assembly Co-occurrence networks

DIW1 encoding a cladeⅠPP2C phosphatase negatively regulates drought tolerance by de-phosphorylating TaSnRK1.1 in wheat

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Journal of Integrative Plant Biology 2023年第8期65卷 1918-1936页

作者： Jingyi Wang chaonan li Long li lifeng Gao Ge Hu Yanfei Zhang Matthew P.Reynolds Xueyong Zhang Jizeng Jia Xinguo Mao Ruilian Jing National Key Facility for Crop Gene Resources and Genetic Improvement/Institute of Crop Sciences Chinese Academy of Agricultural SciencesBeijing 100081China International Maize and Wheat Improvement Center Texcoco 56237Mexico

Drought seriously impacts wheat production(Triticum aestivum L.),while the exploitation and utilization of genes for drought tolerance are *** wilting is a direct reflection of drought tolerance in *** A PP2Cs are abscisic acid(ABA)co-receptors playing vital roles in the ABA signaling pathway,regulating drought ***,the roles of other clade PP2Cs in drought tolerance,especially in wheat,remain largely ***,we identified a gain-of-function drought-induced wilting 1(DIW1)gene from the wheat Aikang 58 mutant library by map-based cloning,which encodes a cladeⅠprotein phosphatase 2C(TaPP2C158)with enhanced protein phosphatase *** analysis of overexpression and CRISPR/Cas9 mutant lines demonstrated that DIW1/TaPP2C158 is a negative regulator responsible for drought *** found that TaPP2C158 directly interacts with TaSnRK1.1 and de-phosphorylates it,thus inactivating the TaSnRK1.1–Ta AREB3 ***2C158 protein phosphatase activity is negatively correlated with ABA *** analysis suggested that C-terminal variation of TaPP2C158 changing protein phosphatase activity is highly correlated with the canopy temperature,and seedling survival rate under drought *** data suggest that the favorable allele with lower phosphatase activity of TaPP2C158 has been positively selected in Chinese breeding *** work benefits us in understanding the molecular mechanism of wheat drought tolerance,and provides elite genetic resources and molecular markers for improving wheat drought tolerance.

关键词： association analysis drought elite genetic resources map-based clone PP2C protein phosphatase wheat wilting

Implications of vitamin D for flesh quality of grass carp(Ctenopharyngodon idella):antioxidant ability,nutritional value,sensory quality,and myofiber characteristics

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Journal of Animal Science and Biotechnology 2023年第6期14卷 2596-2613页

作者： Yao Zhang chaonan li Xiaoqiu Zhou Weidan Jiang Pei Wu Yang liu Hongmei Ren Lu Zhang Haifeng Mi Jiayong Tang Ruinan Zhang lin Feng Animal Nutrition Institute Sichuan Agricultural UniversityChengdu 611130SichuanChina. Fish Nutrition and Safety Production University Key Laboratory of Sichuan Province Sichuan Agricultural UniversityChengdu 611130China. Key Laboratory for Animal Disease‑Resistant Nutrition of China Ministry of Education Sichuan Agricultural UniversityChengdu 611130China. Healthy Aquaculture Key Laboratory of Sichuan Province Tongwei Co.Ltd.Chengdu 610041SichuanChina.

Background Muscle represents a unique and complex system with many components and comprises the major edible part of *** D is a critical nutrient for animals and is known to enhance calcium absorption and immune *** recent years,dietary vitamin D supplementation in livestock has received increased attention due to biological responses including improving shear force in mammalian ***,the vitamin D acquisition and myofiber development processes in fish differ from those in mammals,and the effect of vitamin D on fish flesh quality is poorly ***,the influence of dietary vitamin D on fillet quality,antioxidant ability,and myofiber development was examined in grass carp(Ctenopharyngodon idella).Methods A total of 540 healthy grass carp,with an initial average body weight of 257.24±0.63 g,were allotted in 6 experimental groups with 3 replicates each,and respectively fed corresponding diets with 15.2,364.3,782.5,1,167.9,1,573.8,and 1,980.1 IU/kg vitamin D for 70 *** Supplementation with 1,167.9 IU/kg vitamin D significantly improved nutritional value and sensory quality of fillets,enhancing crude protein,free amino acid,lipid,and collagen contents;maintaining an ideal pH;and reduc-ing lactate content,shear force,and cooking loss relative to respective values in the control(15.2 IU/kg)*** myofiber diameter and the frequency of myofibers>50μm in diameter increased under supplementation with 782.5–1,167.9 IU/kg vitamin *** of oxidative damage biomarkers decreased,and the expression of antioxi-dant enzymes and nuclear factor erythroid 2-related factor 2 signaling molecules was upregulated in the 1,167.9 IU/kg vitamin D treatment compared to respective values in the control ***,vitamin D supplementation activated cell differentiation by enhancing the expression of myogenic regulatory factors and myocyte enhancer factors compared to that in the control *** addition,supplementation with 1,167.9 IU/kg

关键词： Antioxidant Grass carp Myofiber development Nutritional value Sensory quality Vitamin D

Regulation of protein thermal stability and its potential application in the development of thermo-attenuated vaccines

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Engineering Microbiology 2024年第3期4卷 16-21页

作者： Maofeng Wang Cancan Wu Nan liu Xiaoqiong Jiang Hongjie Dong Shubao Zhao chaonan li Sujuan Xu lichuan Gu State Key Laboratory of Microbial Technology Shandong UniversityQingdao 266237China Research Center of Translational Medicine Central Hospital Affiliated to Shandong First Medical UniversityJinan 250013China Department of Orthopedics Shandong Provincial Hospital Affiliated to Shandong First Medical UniversityJinan 250021China Shandong Institute of Parasitic Diseases Shandong First Medical University&Shandong Academy of Medical SciencesJining 272033China

The coronavirus disease 2019(COVID-19)pandemic has highlighted the importance of developing novel *** ideal vaccine should trigger an intense immune reaction without causing significant side *** this study we found that substitution of tryptophan located in the cores of severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)protein structures with certain smaller amino acids resulted in variants with melting temperatures of 33-37℃.An enzyme activity assay indicated that the proteolytic activity of the main proteinase(3CLpro)decreased sharply when the environmental temperature exceeded the melting temperature,implying that other protein variants may lose most of their functions under the same *** finding suggests that a virus variant containing engineered proteins with melting temperatures of 33-37℃ may only be functional in the upper respiratory tract where the temperature is about 33℃,but will be unable to invade internal organs,which maintain temperatures above 37℃,thus making it possible to construct temperature-sensitive attenuated vaccines.

关键词： Protein melting temperature Enzyme activity SARS-CoV-2 N protein 3CL^(pro) Temperature sensitive attenuated vaccines

Establishment and Functional Evaluation of a Rat Model of Spinal Cord Injury

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Journal of Clinical and Nursing Research 2022年第4期6卷 28-34页

作者： Shuiqin li Wei Wei Fan Fan Yang Xue chaonan li Department of Rehabilitation Medicine The First Affiliated Hospital of Xi’an Medical UniversityXi’an 710000Shaanxi ProvinceChina

Objective:To explore the modified Allen impactor method in establishing a rat model of spinal cord injury,and to preliminarily evaluate the motor function of the forelimbs and hindlimbs of ***:Thirty female SD rats with a body weight of 255±21g were randomly divided into two groups,namely the sham-operated group and the operated group,with 15 rats in each *** spinal cord injury SD rat model was established by exposing but not injuring the spinal cord in the sham-operated group,while the SD rat model was established by the modified Allen impactor method in the operated *** Basso-Beattie-Bresnahan(BBB)rating scale was used to assess the rats’hindlimb motor neurobehavior.A rat model of spinal cord injury was established by the modified Allen impactor *** the cells were transplanted,the BBB score was used to evaluate the motor function;the changes in the motor function of rats with spinal cord injury were ***:The motor function and sensory function of the forelimbs and hindlimbs of the rats showed significant changes after five *** motor function of the forelimbs and hindlimbs of the rats in the sham-operated group were essentially normal after three days(about 20 points);the sensory function of the rats in the operated group decreased significantly after five days;however,in the sham-operated group,it decreased to *** motor function scores of the rats in the operated group at each point of time were significantly lower than those in the sham-operated group(plimb motor function scores were significantly higher than those in the sham-operated group(plish a rat model of spinal cord injury in this study can significantly reduce the motor function of rats.

关键词： Spinal cord injury model Motor function Sensory function Modified Allen impactor

Effect of Ketoprofenphonophoresis and Femoral Nerve Block in Knee Osteoarthritis

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Journal of Clinical and Nursing Research 2021年第6期5卷 72-78页

作者： Fan Fan Wei Wei Binyan He chaonan li Qing Yang Shuiqin li Department of Rehabilitation Medicine the First Affiliated Hospital of Xi’an Medical UniversiyXi’an 710077Shaanxi ProvinceChina

Objective:To evaluate the effect of ketoprofenphonophoresis and femoral nerve block in knee ***:One hundred and fourteen patients with knee osteoarthritis were randomly divided into two *** control group consisted of 57 patients who were treated with only *** experimental group consisted of patients were treated with ketoprofenphonophoresis and femoral nerve *** patients were followed for more than one *** treatment effect assessed by observing their WOMAC index before and after ***:All the patients finished the *** WOMAC indexes were reduced after therapy and one month after treatment,especially in the experimental group(P<0.05).The effective efficiency of ketoprofenphonophoresis and femoral nerve block were higher than that in the control group after treatment and after one month of follow-up(P<0.05).Conclusion:Compared with only ketoprofenphonophoresis,the combined treatment of ketoprofenphonophoresis and femoral nerve block was more appropriate in knee osteoarthritis.

关键词： Ketoprofenphonophoresis Femoral nerve block Knee osteoarthritis