检索结果-南通市图书馆

Enzyme-linked immunosorbent assay of changes in serum levels of growth hormone(cGH)in common carps(Cyprinus carpio)

Science China(Life Sciences) 2008年第2期51卷 157-163页

作者： WU Gang CHEN LiHua ZHONG Shan LI Qi song chaojun JIN BoQuan ZHU ZuoYan State Key Laboratory of Freshwater Ecology and Biotechnology Institute of HydrobiologyChinese Academy of SciencesWuhan 430072China Department of Immunology Fourth Military Medical UniversityXi’an 710032China College of Life Science Wuhan UniversityWuhan 430072China

The aim of the present study was to purify the common native carp growth hormone (ncGH), produce monoclonal antibodies (mAbs) to common native carp growth hormone (ncGH), and further enhance the sensitivity of enzyme-linked immunosorbent assays (ELISA) for ncGH. Additionally, we investigated changes in serum ncGH levels in carps raised in different environmental conditions. The recombinant grass carp (Ctenopharyngodon idella) growth hormone was purified and used as antigen to immunize the rabbit. The natural ncGH was isolated from the pituitaries of common carp. SDS-PAGE and Western blot utilizing the polyclonal anti-rgcGH antibody confirmed the purification of ncGH from pituitaries. Purified ncGH was then used as an immunogen in the B lymphocyte hybridoma technique. A total of 14 hybridoma cell lines (FMU-cGH 1-14) were established that were able to stably secrete mAbs against ncGH. Among them, eight clones (FMU-cGH1-6, 12 and 13) were successfully used for Western blot while nine clones (FMU-cGH 1-7, 9 and 10) were used in fluorescent staining and immunohistochemistry. Epitope mapping by competitive ELISA demonstrated that these mAbs recognized five different epitopes. A sensitive sandwich ELISA for detection of ncGH was developed using FMU-cGH12 as the coating mAb and FMU-cGH6 as the enzyme labeled mAb. This detection system was found to be highly stable and sensitive, with detection levels of 70 pg/mL. Additionally, we found that serum ncGH levels in restricted food group and in the net cage group increased 6.9-and 5.8-fold, respectively, when compared to controls, demonstrating differences in the GH stress response in common carp under different living conditions.

关键词： common carp growth hormone monoclonal antibodies ELISA starvation net cage

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Establishment of NaLuF_(4):15%Tb-based low dose X-PDT agent and its application on efficient antitumor therapy

引用

International Journal of Minerals,Metallurgy and Materials 2024年第3期31卷 599-610页

作者： Yi Tian Zhiguang Fu Xiaosheng Zhu Chunjing Zhan Jinwei Hu Li Fan chaojun song Qian Yang Yu Wang Mei Shi Department of Radiation Oncology Xijing HospitalThe Fourth Military Medical UniversityXi’an 710032China Department of Oncology Air Force Medical CenterPLAThe Fourth Military Medical UniversityBeijing 100142China Department of Tumor Radiotherapy Air Force Medical CenterPLAThe Fourth Military Medical UniversityBeijing 100142China Department of Gastroenterology Air Force Hospital of Southern Theater CommandGuangzhou 510062China Department of Pharmaceutical Chemistry and Analysis School of PharmacyThe Fourth Military Medical UniversityXi’an 710032China School of Life Sciences Northwestern Polytechnical UniversityXi’an 710072China Institue of Materia Medica School of PharmacyThe Fourth Military Medical UniversityXi’an 710032China

X-ray excited photodynamic therapy(X-PDT)is the bravo answer of photodynamic therapy(PDT)for deep-seated tumors,as it employs X-ray as the irradiation source to overcome the limitation of light penetration ***,high X-ray irradiation dose caused organ lesions and side effects became the major barrier to X-PDT *** address this issue,this work employed a classic-al co-precipitation reaction to synthesize NaLuF_(4):15%Tb^(3+)(NLF)with an average particle size of(23.48±0.91)nm,which was then coupled with the photosensitizer merocyanine 540(MC540)to form the X-PDT system NLF-MC540 with high production of singlet *** system could induce antitumor efficacy to about 24%in relative low dose X-ray irradiation range(0.1-0.3 Gy).In vivo,when NLF-MC540 irradiated by 0.1 Gy X-ray,the tumor inhibition percentage reached 89.5%±5.7%.The therapeutic mechanism of low dose X-PDT was found.A significant increase of neutrophils in serum was found on the third day after *** immunohistochemical staining of tumor sections,the Ly6G^(+),CD8^(+),and CD11c^(+)cells infiltrated in the tumor microenvironment were *** the bilat-eral tumor model,the NLF-MC540 with 0.1 Gy X-ray irradiation could inhibit both the primary tumor and the distant tumor ***-tected by enzyme linked immunosorbent assay(ELISA),two cytokines IFN-γand TNF-αin serum were upregulated 7 and 6 times than negative control,*** by enzyme linked immune spot assay(ELISPOT),the number of immune cells attributable to the IFN-γand TNF-αlevels in the group of low dose X-PDT were 14 and 6 times greater than that in the negative control group,***,it conclude that low dose X-PDT system could successfully upregulate the levels of immune cells,stimulate the secretion of cy-tokines(especially IFN-γand TNF-α),activate antitumor immunity,and finally inhibit colon tumor growth.

关键词： X-ray excited photodynamic therapy singlet oxygen low dose X-Ray irradiation efficient antitumor therapy anti-tumor immunity

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

A novel colon cancer targeting mAb FMU198 for intracellular negative gold nanoparticles delivery,cancer diagnosis and treatment

A novel colon cancer targeting mAb FMU198 for intracellular ...

引用

第九届全国免疫学学术大会

作者： chaojun song Li Fan Yongzhan Nie Xiaofeng Huang Boquan Jin Department of Immunology the Fourth Military Medical University

Backgrounds:FMU198 recognizes 79%of colon cancers while it is negative when tested on 24 different healthy human tissues analyzed by immunohistochemistry(IHC).Moreover,FMU198 is rapidly internalized when it binds to colon cancer *** drug carriers have been used widely for drug *** gold(Au)particles can loaddrug with high efficency but are hard to cross the cell membrane due to electrostatic repulsion against negative charge protein on the membrane ***,in our study,FMU198 was prepared for intracellular delivery of negative gold ***:Dynamic laser light scattering(DLS)and transmission electron microscopy(TEM)were employed to determine the characteristics of *** cytometry and confocal fluorescence microscopy were used to demonstrate FMU198 mediated cell-selective intracellular delivery of the Au *** order to demonstrate the targeting efficiency of FMU198 in vivo,bio-imaging study was conducted using in vivo fluorescence imaging system in Colo205tumor bearing nude ***:The size of FMU198 coated Au nanoparticles with spherical morphology is about 20 nm according to the DLS results with negative zeta potential and the result also indicated that the system could be stable both in PBS and medium with *** TEM results revealed that in case of Au-FMU198,the intracellular gold amount was significantly increased in cells compared to Au-isotype which showed very low *** cytometry and confocal fluorescence microscopy showed that the intracellular uptake of negatively charged particle is enhanced to a greater extent using Au-FMU198 as delivery *** vivo bio-imaging study demonstrated that the fluorescence intensity of Au-(Cy5)-FMU198 treated group is8.4-fold higher than that of Au-(Cy5)-isotype treated ones upon 24 h post ***:Therefore,we demonstrated the use of novel tumor targeting molecular FMU198 for intracellular negative gold nanoparticles delivery and t

关键词： colon cancer negative gold nanoparticles intracellular delivery

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

近代民间教育团体农村职业教育的实施——以中华职业教育社为例

引用

职业技术教育 2024年第33期45卷 73-80页

作者：崔军伟宋超军中国石油大学(华东)马克思主义学院青岛266580

1920年后,中华职业教育社将工作重心转向农村职业教育。为将农村职业教育工作落到实处,中华职业教育社选定江苏昆山徐公桥创设实验区,在教育上普及义务教育,推广民众教育;在经济上推广农业改良,提倡副业生产,开展合作运动。另外,改进实... 详细信息

1920年后,中华职业教育社将工作重心转向农村职业教育。为将农村职业教育工作落到实处,中华职业教育社选定江苏昆山徐公桥创设实验区,在教育上普及义务教育,推广民众教育;在经济上推广农业改良,提倡副业生产,开展合作运动。另外,改进实验区还创设农村自治组织,举办人才培训机构。中华职业教育社的农村职业教育实验活动关注农民需求,讲求经济节省,强调因人施教,注重制度建设,既推动了改进区农村职业教育及各方面的进步,也为其他改进区农村职业教育的实施提供了典范,还为民国长江三角洲地区的乡村建设运动打下了良好基础。

关键词：农村职业教育中华职业教育社徐公桥改进实验区

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Expression profile of NLRs and ALR in human colorectal cancer: Correlation with cancer stages and inflammasome components

Expression profile of NLRs and ALR in human colorectal cance...

引用

第十届全国免疫学学术大会

作者： rongrong liu Agnieszka Truax Peizhen Hu Zengshan Li chaojun song Lihua Chen Ting Jenny Pan-Yun Department of Immunology School of Basic Medicine Fourth Military Medical University The Lineberger Comprehensive Cancer Center University of North Carolina at Chapel Hill Department of Pathology Xijing Hospital Fourth Military Medical University

Purpose:NLRs(nucleotide-binding domain leucine-rich repeat proteins or NOD-like receptors)are regulators of inflammation and immunity.A subgroup of NLRs and the innate immune receptor,AIM2(absent-in-melanoma 2),can induce the assembly of a large caspase-1 activating complex called the *** NLRs regulate key signaling pathways such as NFk B and *** inflammation is a central component of colorectal cancer(CRC),this work was

关键词： NLRs AIM2 FFPE colorectal cancer Inflammasome

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

晋北地区油松大树移植成活机理及技术措施

引用

山西科技 2010年第6期25卷 143-145页

作者：宋朝军山西省大同市绿化委员会办公室山西大同037000

从油松生态特性出发,分析了油松大树移植原理;从树木移植前的准备、移植、移植后的管护3个方面阐述了油松大树移植技术,有助于提高油松大树移植的成活率。

关键词：晋北地区油松大树移植技术

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

EpCAM(EpEx/Ep-ICD) expression and clinical correlation:potential diagnostic biomarker of pancreatic cancer

EpCAM(EpEx/Ep-ICD) expression and clinical correlation:poten...

引用

第八届全国免疫学学术大会

作者： Kui Zhang chaojun song Yongming Li Xin Xie Cansheng Zhu Yuanjie Sun Ping Zhu Kun Yang Boquan Jin Department of Clinical Immunology State Key Discipline of Cell Biology Xijing Hospital Fourth Military Medical University Department of Immunology Fourth Military Medical University

Background and Purpose:Pancreatic cancer is a serious disease with high cancer-related deaths *** biomaker molecules on tissue was formally accepted as biomaker of pancreatic cancer to diagnose or treat this *** this study, EpCAM expression pattern was detected on pancreatic cancer tissue, and further analyzed it’s relation with clinical data in order to find the role in guiding the diagnosis and EpCAM-target therapy of pancreatic cancer.

关键词： EpCAM EpEx Ep-ICD pancreatic cancer

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

CD226 ligation protects against EAE by promoting IL-10 expression via regulation of CD4+ T cell differentiation

CD226 ligation protects against EAE by promoting IL-10 expre...

引用

第十届全国免疫学学术大会

作者： Hanyu Zeng Rong Zhang Yun Zhang Chunmei Zhang chaojun song Liang Fang Ran Zhuang Zhuwei Xu Kun Yang Boquan Jin Lihua Chen Department of Immunology the Fourth Military Medical University

IL-10 is an important anti-inflammatory cytokine that has been implicated in a variety of autoimmune *** we show that CD226 ligation by anti-CD226 m Ab Leo A1 efficiently promoted IL-10 production in human peripheral ... 详细信息

关键词： CD226 IL-10 EAE

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

民国时期陆叔昂的农村改进思想对乡村振兴战略的启示

引用

岭南师范学院学报 2024年第1期45卷 52-58页

作者：宋超军姜缘中国石油大学(华东)马克思主义学院山东青岛266580

陆叔昂详细论述农村改进的起源、内容和组织模式,阐明乡村建设运动的前因后果,并强调农村改进既需要农民的积极主动参与,也离不开专门的农村改进人才的带动、指导。陆叔昂的农村改进思想为当下的乡村建设提供了启示:乡村振兴应以农民为... 详细信息

陆叔昂详细论述农村改进的起源、内容和组织模式,阐明乡村建设运动的前因后果,并强调农村改进既需要农民的积极主动参与,也离不开专门的农村改进人才的带动、指导。陆叔昂的农村改进思想为当下的乡村建设提供了启示:乡村振兴应以农民为中心,激发农民主体意识;乡村振兴既要推动农村经济发展,也要提升农民素质素养;乡村振兴应重视人才作用,培养更多乡村自治人才;乡村振兴应因时因地制宜、分类指导、精准施策。

关键词：民国时期陆叔昂农村改进思想乡村振兴战略 “富教兼施”

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

中华职业教育社农村教育思想及实践对乡村教育发展的启示

引用

江苏高职教育 2023年第4期23卷 50-55,78页

作者：宋超军崔军伟中国石油大学(华东)马克思主义学院山东青岛266580

20世纪20年代末,中华职业教育社事业重心由职业教育逐渐转向农村改进,教育成为其农村改进事业发展的重要途径。职教社先行者们在江苏昆山徐公桥设立改进实验区,从普及义务教育、推广民众教育和加强青年训练三方面对试验区教育进行了改造... 详细信息

20世纪20年代末,中华职业教育社事业重心由职业教育逐渐转向农村改进,教育成为其农村改进事业发展的重要途径。职教社先行者们在江苏昆山徐公桥设立改进实验区,从普及义务教育、推广民众教育和加强青年训练三方面对试验区教育进行了改造,既提高了农民的素质素养,也促进了当地的区域发展。职教社农村教育思想及实践为当前乡村教育的发展提供了启示:农村教育应因人施教,各种教育齐头并进;农村教育既要提高农民致富能力,也要丰富农民精神生活;农村教育应立足农村发展实际,切实维护农民群众利益;农村教育应重视青年人才作用,培养更多乡村自治人才。

关键词：民国时期中华职业教育社农村教育

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：