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EPS及消毒剂对细菌表面附着和初期生物膜形成的影响

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净水技术 2024年第2期43卷 70-76页

作者：钟疏影余超陈国炜蔡寅诺刘丽合肥工业大学土木与水利工程学院安徽合肥230009

为探究胞外聚合物(extracellular polymeric substances, EPS)和消毒剂对细菌表面附着聚集行为的影响,文中以野生型铜绿假单胞菌和藻酸盐分泌过量的突变株为研究对象,分析不同氯浓度条件下细菌EPS生成及其对表面附着和初期生物膜形成的... 详细信息

为探究胞外聚合物(extracellular polymeric substances, EPS)和消毒剂对细菌表面附着聚集行为的影响,文中以野生型铜绿假单胞菌和藻酸盐分泌过量的突变株为研究对象,分析不同氯浓度条件下细菌EPS生成及其对表面附着和初期生物膜形成的影响。结果表明,相较于野生型,突变型的生物膜形成优势显著,特别是当氯质量浓度≥1.0 mg/L时,强化了其形成优势;EPS也显著提高了生物膜的黏附强度以及降低其表面粗糙程度。低浓度氯促进细菌EPS的生成以及初期生物膜的形成,但高质量浓度氯(3.0 mg/L)则抑制了生物膜的形成,同时也降低了两种菌株生物膜的黏附力与表面粗糙度。对于两种菌株而言,聚氯乙烯(PVC)管材上生物膜的形成优势显著高于不锈钢(STS)管材。

关键词：消毒剂生物膜胞外聚合物(EPS) 表面附着生物膜粗糙度

椎旁肌功能横截面积预测骨质疏松性椎体压缩骨折

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中国组织工程研究 2024年第33期28卷 5315-5319页

作者：张文胜宋振杰郭海威吴春飞杨瀚迪李颖李文超刘洪江杨晓光原超广东省中医骨伤研究院广东省广州市510405 广州中医药大学第三附属医院广东省广州市510405

背景:骨质疏松性椎体压缩骨折是继发于骨质疏松症的常见骨折,目前尚缺乏有效的骨质疏松性椎体压缩骨折预测指标及方法。目的:探讨椎旁肌退变、功能横截面积及脂肪浸润百分比对骨质疏松性椎体压缩骨折的预测作用。方法:纳入2.18年1月至2...

背景:骨质疏松性椎体压缩骨折是继发于骨质疏松症的常见骨折,目前尚缺乏有效的骨质疏松性椎体压缩骨折预测指标及方法。目的:探讨椎旁肌退变、功能横截面积及脂肪浸润百分比对骨质疏松性椎体压缩骨折的预测作用。方法:纳入2.18年1月至2.2.年6月确诊的骨质疏松患者2.4例,随访时间2.以上,根据是否发生椎体骨折分为骨折组和非骨折组,收集患者人口学资料、体质量指数、椎体骨密度等详细信息,测量并计算L2.体下终板层面双侧腰大肌及背伸肌(竖脊肌和多裂肌)功能横截面积和脂肪浸润百分比。结果与结论:①2.4例患者中有12.例发生骨折为骨折组,98例未发生骨折为非骨折组,两组年龄、性别、身高、体质量、体质量指数、骨折节段比较差异均无显著性意义(P>0.05);②骨折组骨密度显著低于非骨折组(P0.05);③受试者操作特征曲线分析显示椎体骨密度、背伸肌脂肪浸润百分比、背伸肌功能横截面积和腰大肌脂肪浸润百分比的截断值分别为0.903 g/cm2.35.42.%,418.875 mm2.6.375%;曲线下面积分别为0.634,0.755,0.876,0.585;④结果表明:椎旁肌退变与骨质疏松性椎体压缩骨折的发生密切相关,背伸肌功能横截面积可以有效预测骨质疏松性椎体压缩骨折的发生,有助于早期防治骨质疏松性椎体压缩骨折。

关键词：椎旁肌功能横截面积骨质疏松腰椎骨折预测

ZnF_(2.coated three dimensional li-Ni composite anode for improved performance

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Journal of Materiomics 2019年第2期5卷 176-184页

作者： Weishang Jia Ying Wang Siji Qu Zeyu Yao Yuchi liu chao li Zhihong Wang Jingze li School of Materials and Energy University of Electronic Science and Technology of ChinaChengdu610054China School of Mechanical and Automotive Engineering Shanghai University of Engineering Science333 Longteng RoadSongjiang DistrictShanghai201620China

lithium metal is a promising anode material for high energy density batteries,which is restricted from practical application by the issues including lithium dendrite,parasitic reaction and volumetric ***,a strategy combing bulk and surface modification is proposed to address these *** bulk modification is to use Ni foam as three dimensional(3D)scaffold for direct infusion of molten li resulting in the formation of li-Ni composite anode,which reduces actual current density by increasing specific surface area and alleviates volumetric change in the process of li stripping/*** the surface modification is to coat with ZnF_(2.film via sputtering,which is acted as artificial protective layer for controlling interfacial side *** symmetric cell consisting of ZnF_(2.coated li-Ni composite electrodes exhibits low overpotential(about 45 mV)and stable cycling over 900 h at 1mA cm^(-2..Furthermore,the cell with liCoO_(2.cathode delivers 110 mAh g^(-1)at 1 C rate after 500 cycles.

关键词： lithium metal anode Composite lithium Nickel foam lithium dendrite Surface modification lithium battery

Dynamic changes in intramedullary pressure 72.hours after spinal cord injury

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Neural Regeneration Research 2019年第5期14卷 886-895页

作者： Xin Zhang Chang-Bin liu De-Gang Yang Chuan Qin Xue-chao Dong Da-Peng li chao Zhang Yun Guo liang-Jie Du Feng Gao Ming-liang Yang Jian-Jun li School of Rehabilitation Medicine Capital Medical University Department of Spinal and Neural Function Reconstruction China Rehabilitation Research Center Center of Neural Injury and Repair Beijing Institute for Brain Disorders China Rehabilitation Science Institute Beijing Key Laboratory of Neural Injury and Rehabilitation

Intramedullary pressure increases after spinal cord injury, and this can be an important factor for secondary spinal cord injury. Until now there have been no studies of the dynamic changes of intramedullary pressure after spinal cord injury. In this study, telemetry systems were used to observe changes in intramedullary pressure in the 72.hours following spinal cord injury to explore its pathological mechanisms. Spinal cord injury was induced using an aneurysm clip at T10 of the spinal cord of 30 Japanese white rabbits, while another 32.animals were only subjected to laminectomy. The feasibility of this measurement was assessed. Intramedullary pressure was monitored in anesthetized and conscious animals. The dynamic changes of intramedullary pressure after spinal cord injury were divided into three stages: stage I(steep rise) 1–7 hours, stage Ⅱ(steady rise) 8–38 hours, and stage Ⅲ(descending) 39–72.hours. Blood-spinal barrier permeability, edema, hemorrhage, and histological results in the 72.hours following spinal cord injury were evaluated according to intramedullary pressure changes. We found that spinal cord hemorrhage was most severe at 1 hour post-spinal cord injury and then gradually decreased; albumin and aquaporin 4 immunoreactivities first increased and then decreased, peaking at 38 hours. These results confirm that severe bleeding in spinal cord tissue is the main cause of the sharp increase in intramedullary pressure in early spinal cord injury. Spinal cord edema and blood-spinal barrier destruction are important factors influencing intramedullary pressure in stages Ⅱ and Ⅲ of spinal cord injury.

关键词： nerve regeneration secondary spinal cord injury telemetry pathological mechanism rabbit conscious anesthetized hemorrhage edema pressure measurement blood-spinal barrier neural regeneration

CUDA-NP： Realizing Nested Thread-Level Parallelism in GPGPU Applications

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Journal of Computer Science & Technology 2015年第1期30卷 3-19页

作者：杨毅李超周辉阳 Department of Computing Systems Architecture NEC Laboratories America Princeton NJ 08540 U.S.A. Department of Electrical and Computer Engineering North Carolina State University Raleigh NC 27606 U.S.A.

Parallel programs consist of series of code sections with different thread-level parallelism （TLP）. As a result, it is rather common that a thread in a parallel program, such as a GPU kernel in CUDA programs, still contains both sequential code and parallel loops. In order to leverage such parallel loops, the latest NVIDIA Kepler architecture introduces dynamic parallelism, which allows a GPU thread to start another GPU kernel, thereby reducing the overhead of launching kernels from a CPU. However, with dynamic parallelism, a parent thread can only communicate with its child threads through global memory and the overhead of launching GPU kernels is non-trivial even within GPUs. In this paper, we first study a set of GPGPU benchmarks that contain parallel loops, and highlight that these benchmarks do not have a very high loop count or high degree of TLP. Consequently, the benefits of leveraging such parallel loops using dynamic parallelism are too limited to offset its overhead. We then present our proposed solution to exploit nested parallelism in CUDA, referred to as CUDA-NP. With CUDA-NP, we initially enable a high number of threads when a GPU program starts, and use control flow to activate different numbers of threads for different code sections. We implement our proposed CUDA-NP framework using a directive-based compiler approach. For a GPU kernel, an application developer only needs to add OpenMP-like pragmas for parallelizable code sections. Then, our CUDA-NP compiler automatically generates the optimized GPU kernels. It supports both the reduction and the scan primitives, explores different ways to distribute parallel loop iterations into threads, and efficiently manages on-chip resource. Our experiments show that for a set of GPGPU benchmarks, which have already been optimized and contain nested parallelism, our proposed CUDA-NP framework further improves the performance by up to 6.69 times and 2.01 times on average.

关键词： GPGPU nested parallelism compiler local memory

Divergence, evolution and adaptation in ray-finned fish genomes

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Science China(life Sciences) 2019年第8期62卷 1003-1018页

作者： chao Bian Yu Huang Jia li Xinxin You Yunhai Yi Wei Ge Qiong Shi Center of Reproduction Development and AgingFaculty of Health SciencesUniversity of MacaoMacaoChina Shenzhen Key Lab of Marine Genomics Guangdong Provincial Key Lab of Molecular Breeding in Marine Economic AnimalsBGI Academy of Marine SciencesBGI MarineBGIShenzhen518083China Laboratory of Aquatic Genomics College of Life Sciences and OceanographyShenzhen UniversityShenzhen518060China

With the rapid development of next-generation sequencing technologies and bioinformatics, over 50 ray-finned fish genomes by far have been sequenced with high quality. The genomic work provides abundant genetic resources for deep understanding of divergence, evolution and adaptation in the fish genomes. They are also instructive for identification of candidate genes for functional verification, molecular breeding, and development of novel marine drugs. As an example of other omics data, the Fish-T1 K project generated a big database of fish transcriptomes to integrate with these published fish genomes for potential applications. In this review, we highlight the above-mentioned recent investigations and core topics on the ray-finned fish genome research, with a main goal to obtain a deeper understanding of fish biology for theoretical and practical applications.

关键词： ray-finned fish genome transcriptome application

Decreasing nitrogen assimilation under drought stress by suppressing DST-mediated activation of Nitrate Reductase 1.2.in rice

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Molecular Plant 2022年第1期15卷 167-178页

作者： Mei-ling Han Qiao-Yan Lv Jing Zhang Tao Wang chao-Xing Zhang Ru-Jiao Tan Ya-ling Wang li-Yuan Zhong Yi-Qun Gao Zhen-Fei chao Qian-Qian li Gen-Yun Chen Zai Shi Hong-Xuan lin Dai-Yin chao National Key Laboratory of Plant Molecular Genetics CAS Centre for Excellence in Molecular Plant SciencesShanghai Institute of Plant Physiology and EcologyChinese Academy of SciencesShanghai 200031China University of Chinese Academy of Sciences Beijing 100049China Henan University Kaifeng 475004China Jiangsu Normal University Xuzhou 221116China

Nitrogen is an essential nutrient for plant growth and development,and plays vital roles in crop *** of nitrogen is thus fine-tuned in response to heterogeneous ***,the regulatory mechanism underlying this essential process remains largely ***,we report that a zinc-finger transcription factor,drought and salt tolerance(DST),controls nitrate assimilation in rice by regulating the expression of *** found that loss of function of DSTresults in a significant decrease of nitrogen use efficiency(NUE)in the presence of *** revealed that DST is required for full nitrate reductase activity in rice and directly regulates the expression of OsNR1.2.a gene showing sequence similarity to nitrate *** genetics and biochemistry studies revealed that OsNR1.2.encodes an NADH-dependent nitrate reductase that is required for high NUE of ***,the DST-OsNR1.2.regulatory module is involved in the suppression of nitrate assimilation under drought stress,which contributes to drought *** the negative role of DST in stomata closure,as revealed previously,the positive role of DST in nitrogen assimilation suggests a mechanism couplingni-trogen metabolism and stomata *** discovery of this coupling mechanism will aid the engi-neering of drought-tolerant crops with high NUE in the future.

关键词： nitrogen use efficiency OsNR1.2 drought tolerance rice

Patterns of gas hydrate accumulation in mass transport deposits related to canyon activity: Example from Shenhu drilling area in the South China Sea

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Acta Oceanologica Sinica 2019年第5期38卷 118-128页

作者： chao Fu Shengli li Xinghe Yu Jinqiang liang Zenggui Kuang Yulin He lina Jin School of Energy Resources China University of Geosciences Guangzhou Marine Geological Survey China Geological Survey

Since 2.17, a plenty of gas hydrates have been drilled in a new area of Shenhu, and good heterogeneity has been found throughout the spatial distribution of the reservoir. After distinguishing different sedimentary sequence types and matching their formation with slope deposition settings, this study proposes three mass transport deposit(MTD) patterns related to canyon activity that occurred contemporaneously or epigenetically with it: well preserved MTDs, MTDs eroded by canyon migration, and MTDs dislocated by contemporaneous faults. Based on seismic reflection characteristics, this study proposed methods of quantitatively analyzing sedimentary factors,such as measuring the turbidities flow rate in the canyon, and results are interpreted with respect to canyon activity. Combining the above parameters and their relationship with gas hydrate accumulation, fine-grained seals overlapping coarse MTDs reservoirs are found to be indispensable to gas hydrate accumulation, as they prevent the release of free gas. Based on grain size data of hydrate samples from drilling wells, multi-layered gas hydrate reservoirs capped by fine-grained sediments and overlapping mud show favorable hydrate-bearing prospects. The release of gas hydrates, however, is mostly caused by the lack of mud sealing in relation to canyon activity, such as turbidities flow erosion and contemporaneous fault breaking. Canyon migration with respect to MTDs may be the actual cause of erosion of overlapping syn-sedimentary layers, and high bottom flows may contribute to an increase in the release of free gas. It is believed that contemporaneous faults caused by unstable canyon walls may break the muddy over layers and decrease the accumulation pressure of gas hydrate ***, according to the sedimentary characteristics of MTDs and the hydrate accumulation process, three responding accumulation or releasing patterns are proposed, which respond to the different types of MTDs distinguished above: a well-pr

关键词： South China Sea gas hydrate sedimentary characteristics MTDs hydrate responding pattern

New Re-Os Isotopic Constrains on the Formation of the Metalliferous Deposits of the Lower Cambrian Niutitang Formation

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Journal of Earth Science 2016年第2期27卷 271-281页

作者： Yong Fu lin Dong chao li Wenjun Qu Haoxiang Pei Wenlang Qiao Bing Shen College of Resource and Environmental Engineering Guizhou University Guiyang 550012 China Key Laboratory of Metallogeny and Mineral Assessment Institute of Mineral Resources Chinese Academy of Geological Sciences Beijing 100037 China Key Laboratory of Orogenic Belts and Crustal Evolution MOE School of Earth and Space Science Peking University Beijing 100871 China Re-Os lab National Research Center for Geoanalysis Chinese Academy of Geological Sciences Beijing 100037 China Institute of Guizhou Geological Survey Guiyang 550004 China

The Terreneuvian Epoch（541-52. Ma）is also an important period for metallogenesis in South China, as was represented by the widespread occurrences of Ni-Mo polymetallic layers on the antecedent shallow platform margin and the V-enriched black shales in deeper slope-basin settings. In this study, we have measured Re-Os isochron ages of Ni-Mo polymetallic layers（Songlin, Niuchang, Sancha, Chuanpengwan）, V-rich black shales（Bahuang）, and non-metalliferous black shales（Shuidong） in the basal Niutitang Formation in Guizhou and Hunan province, South China. The Ni-Mo polymetallic layers and V-enriched black shales have similar Re-Os isochron ages, suggesting concurrent deposition of these two types of metalliferous ores. This suggestion is consistent with the traditional stratigraphic correlation by using the nodular phosphorite bed directly underlying these metalliferous layers as a stratigraphic marker. Furthermore, the metalliferous ores and non-metalliferous black shales have similar initial ^（187）Os/^（188） Os ratios of 0.8-0.9, arguing for a dominant seawater origin with minor contribution of hydrothermal activity. Furthermore, Re-Os isotopic data also imply that Ni-Mo and V ore might have derived from the same source. We suggest that the spatial distribution of metalliferous ores can be explained by the development of non-sulfidic anoxic-suboxic wedge（NSASW） in the slope-basin and sulfidic wedge in the previous platform margin. Upwelling of deep water first transects the mildly reduced, organic rich NSASW, in which V（V） is reduced to V（IV）, and is preferentially removed from seawater by organometallic complex formation. As a result, V-rich black shale deposits in the slope-basin of Yangtze Platform. Further movement of deep water into the sulfidic platform margin results in Ni-Mo polymetallic layer formation.

关键词： Niutitang Formation South China Ni-Mo polymetallic layer V-rich black shale Re-Osisochron ages.