检索结果-南通市图书馆

Activation of AMPK/MnSOD signaling mediates anti-apoptotic effect of hepatitis B virus in hepatoma cells

在线全文

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2016年第17期22卷 4345-4353页

作者： Lei Li Hong-Hai Hong Shi-Ping Chen Cai-Qi Ma Han-Yan Liu Ya-chao yao Key Laboratory for Major Obstetric Diseases of Guangdong Province Key Laboratory of Reproduction and Genetics of Guangdong Higher Education Institutes The Third Affiliated Hospital of Guangzhou Medical University Department of Biochemistry Zhongshan School of Medicine Sun Yat-sen University Department of Clinical Laboratory Medicine the Second People’s Hospital of Guangdong Province

AIM: To investigate the anti-apoptotic capability of the hepatitis B virus(HBV) in the HepG2 hepatoma cell line and the underlying ***: Cell viability and apoptosis were measured by MTT assay and flow cytometry, respectively. Targeted knockdown of manganese superoxide dismutase(Mn SOD), AMP-activated protein kinase(AMPK) and hepatitis B virus X protein(HBx) genes as well as AMPK agonist AICAR and antagonist compound C were employed to determine the correlations of expression of these ***: HBV markedly protected the hepatoma cells from growth suppression and cell death in the condition of serum deprivation. A decrease of superoxide anion production accompanied with an increase of Mn SOD expression and activity was found in Hep G2.215 cells. Moreover, AMPK activation contributed to the up-regulation of Mn SOD. HBx protein was identified to induce the expression of AMPK and Mn SOD. CONCLUSION: Our results suggest that HBV suppresses mitochondrial superoxide level and exerts an antiapoptotic effect by activating AMPK/Mn SOD signaling pathway, which may provide a novel pharmacological strategy to prevent HCC.

关键词： Hepatitis B virus Reactive oxygen species Apoptosis Manganese superoxide dismutase AMP-activated protein kinase

A Knowledge Model System for Wheat Production Management

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Pedosphere 2007年第2期17卷 172-181页

作者： ZHU Yan CAO Wei-Xing DAI Ting-Bo TIAN Yong-chao yao Xia Hi-Tech Key Laboratory of Information Agriculture of Jiangsu Province Nanjing Agricultural University Nanjing 210095 （China）. Key Laboratory of Crop Growth Regulation of Ministry of Agriculture Nanjing Agricultural University Nanjing 210095 （China）

A knowledge model with temporal and spatial characteristics for the quantitative design of a cultural pattern in wheat production, using systems analysis and dynamic modeling techniques, was developed for wheat management, as a decision-making tool in digital farming. The fundamental relationships and algorithms of wheat growth indices and management criteria to cultivars, ecological environments, and production levels were derived from the existing literature and research data to establish a knowledge model system for quantitative wheat management using Visual C^＋＋. The system designed a cultural management plan for general management guidelines and crop regulation indices for timecourse control criteria during the wheat-growing period. The cultural management plan module included submodels to determine target grain yield and quality, cultivar choice, sowing date, population density, sowing rate, fertilization strategy, and water management, whereas the crop regulation indices module included submodels for suitable development stages, dynamic growth indices, source-sink indices, and nutrient indices. Ewluation of the knowledge model by design studies on the basis of data sets of different eco-sites, cultiwrs, and soil types indicated a favorable performance of the model system in recommending growth indices and management criteria under diverse conditions. Practical application of the knowledge model system in comparative field experiments produced yield gains of 2.4% to 16.5%. Thus, the presented knowledge model system overcame some of the difficulties of the traditional wheat management patterns and expert systems, and laid a foundation for facilitating the digitization of wheat management.

关键词： expert system knowledge model quantitative decision-making regulation index wheat

Multifunctional role of oral bacteria in the progression of nonalcoholic fatty liver disease

在线全文

学校读者我要写书评

暂无评论

World Journal of Hepatology 2024年第5期16卷 688-702页

作者： En-Hua Mei chao yao Yi-Nan Chen Shun-Xue Nan Sheng-Cai Qi Shanghai Medical College Fudan UniversityShanghai 200000China Department of Prothodontics Shanghai Stomatological HospitalFudan UniversityShanghai 200000China Shanghai Key Laboratory of Craniomaxiofacial Development and Diseases Fudan UniversityShanghai 200000China

Non-alcoholic fatty liver disease(NAFLD)encompasses a spectrum of liver disorders of varying severity,ultimately leading to *** spectrum primarily consists of NAFL and non-alcoholic *** pathogenesis of NAFLD is closely associated with disturbances in the gut micr-obiota and impairment of the intestinal ***-gut commensal flora,particularly bacteria,play a pivotal role in the progression of ***,Porphyromonas gingivalis,a principal bacterium involved in periodontitis,is known to facilitate lipid accumulation,augment immune responses,and induce insulin resistance,thereby exacerbating fibrosis in cases of periodontitis-associated *** influence of oral microbiota on NAFLD via the“oral-gut-liver”axis is gaining recognition,offering a novel perspective for NAFLD management through microbial imbalance *** review endeavors to encapsulate the intricate roles of oral bacteria in NAFLD and explore underlying mechanisms,emphasizing microbial control strategies as a viable therapeutic avenue for NAFLD.

关键词： Non-alcoholic fatty liver disease Oral bacteria Gut bacteria Periodontitis Non-alcoholic steatohepatitis

Computed tomography imaging and clinical significance of bacterium-positive pulmonary tuberculosis complicated with diabetes

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Clinical Cases 2024年第20期12卷 4230-4238页

作者： Xue-Song Rong chao yao Section 6 Department of TuberculosisAnhui Chest HospitalHefei 230022Anhui ProvinceChina

BACKGROUND The increasing prevalence of tuberculosis(TB)and diabetes on a global scale poses a significant health challenge,particularly due to their co-occurrence,which amplifies the severity,recurrence and mortality rates associated with both *** highlights the need for further investigation into their *** To explore the computed tomography(CT)imaging and clinical significance of bacterium-positive pulmonary TB(PTB)combined with *** There were 50 patients with bacterium-positive PTB and diabetes,and 50 with only bacterium-positive *** latter were designated as the control *** CT imaging of the two groups of patients was compared,including lesion range,shape,density and *** No significant differences were observed in age,gender,smoking and drinking history,high blood pressure,hyperlipidemia and family genetic factors between the ***,compared to the patients diagnosed solely with simple bacterium-positive PTB,those with concurrent diabetes showed a wider range of lesions and more complex and diverse morphology on CT *** them,intrapulmonary tuberculosis lesions were often accompanied by manifestations of pulmonary infection,such as cavity formation and *** the same time,diabetes-related signs were often seen on CT images,such as pulmonary infection combined with diabetic pulmonary *** regression analysis identified age and medical history as significant factors influencing the degree of pulmonary infection and CT imaging outcomes in patients with both TB and *** suggests that older age and specific medical histories may increase the risk or severity of pulmonary damage in these *** CT imaging reveals more complex lesions in PTB patients with diabetes,emphasizing the need for careful evaluation and comprehensive analysis to enhance diagnostic accuracy.

关键词： Bacteria-positive pulmonary tuberculosis Diabetes Computed tomography Bronchiectasis

Properties of seven-filament Cu/Ag-sheathed(Ba,K)Fe_(2)As_(2)tapes fabricated from round and square wires

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Rare Metals 2021年第12期40卷 3651-3659页

作者： Zhen-Kun Jin Cong Liu chao yao Liu Li He Huang Dong-Liang Wang Chi-Heng Dong Kai Wang Xian-Ping Zhang Satoshi Awaji Yan-Wei Ma Key Laboratory of Applied Superconductivity Institute of Electrical EngineeringChinese Academy of SciencesBeijing 100190China School of Electronic Electrical and Communication EngineeringUniversity of Chinese Academy of SciencesBeijing 100049China High Field Laboratory for Superconducting Materials Institute for Materials ResearchTohoku UniversitySendai 980-8577Japan

Iron-based superconductor,a promising candidate for high-field applications,has attracted significant *** practical utilization,the superconducting tapes should have high transport critical current density(J_(c)),low production cost,larger mechanical strength and good *** this study,seven-filament Cu/Agsheathed(Ba,K)Fe_(2)As_(2)tapes were fabricated from round and square *** is found that the tapes rolled from square wires have a desirable uniformity of filament deformation and distribution,compared with the ones rolled from round ***,the J_c of tape was obviously improved by using square drawing dies instead of traditional round *** the tapes rolled from square wires annealed under ambient and 2 MPa by hot pressing,the transport J_c values reached 1.51×10^(4) A·cm^(-2)and 4.86×10^(4) ***^(-2)(4.2K,10T),*** deformation process on the cross section of the tapes during rolling was analyzed by using the powder-flow *** results indicate a potential application of Cu/Ag(Ba,K)Fe_(2)As_(2)tapes in high-magnetic-field region.

关键词： Iron-based superconductor Multi-filament tape Cu/Ag sheath Square drawing dies Critical current density Powder-flow model

A New Peptide with Membrane-permeable Function Derived from Human Circadian Proteins

在线全文

学校读者我要写书评

暂无评论

Acta Biochimica et Biophysica Sinica 2004年第9期36卷 629-636页

作者： Tao PENG, Ying-Hui LIU, Chun-Lei YANG, chao-Min WAN, yao-Qi WANG, and Zheng-Rong WANG* Department of Biomedical Engineering, School of Basic Medicine and Forensic, Sichuan University, Chengdu 610041, China Department of Biomedical Engineering School of Basic Medicine and ForensicSichuan UniversityChengdu 610041China

Basic peptides such as human immunodeficiency virus type 1 (HIV-1) Tat-(48-60) and Droso- phila Antennapedia-(43-58) have been reported to have a membrane permeability and a carrier function for intracellular protein delivery. Based on the fluorescence microscopic observations of the vascular endothelial cells (ECV-304) and the primary cultured neuroglial cells, we found that human Clock protein DNA-binding peptide [residue 35–47, hClock-(35-47)] had a translocation activity very similar to Tat-(48-60). The cellular uptake of hClock-(35-47) increases with the increase of incubation time and concentration. The internalization effect at 4 °C was same as that at 37 °C. Internalization of hClock-(35-47) was saturable and could be inhibited by the excess of the other MPPs. Moreover, The uptake of these peptides were significantly inhibited in the presence of heparan sulfate. These results strongly suggested that the hClock-(35-47) shared a common or very similar internalization pathway with other MPPs. Furthermore, we injected rat through the common carotid artery with hClock-(35-47)-FITC peptide, and cryostat sections of the brain were prepared and observed using a fluorescence microscope. Result showed that the peptide had the ability to translocate through the blood-brain barrier. It is promising to provide a new safe carrier for the intracellular and encephalic treatment.

关键词： membrane-permeable peptide (MPP) cell membrane hClock DNA-binding peptide blood-brain barrier

Theoretical exploration of water injection gravity flooding oil in ultra-deep fault-controlled fractured-cavity carbonate reservoirs

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Petroleum Exploration and Development 2022年第1期49卷 133-143页

作者： YANG Xuewen WANG Rujun DENG Xingliang LI Shiyin ZHANG Hui yao chao PetroChina Tarim Oilfield Company Korla 841000China

Based on the analysis of geological characteristics of ultra-deep fault-controlled fracture-cavity carbonate reservoirs and division of reservoir units, two physical models were made, and physical simulations of oil displacement by water injection were carried out to find out water flooding mechanism in the fault-controlled fracture-cavity carbonate reservoir under complex flow state. On this basis, a mathematical model of fault-controlled carbonate reservoir with coexisting seepage and free flow has been established. Pilot water injection tests have been carried out to evaluate the effects of enhancing oil recovery by water injection. The results show that: fault-controlled fracture-cavity carbonate reservoir units can be divided into three types:the strong natural energy connected type, the weak natural energy connected type and the weak natural energy isolated type;the fault-fracture activity index of the fault-controlled fractured-cavity body can effectively characterize the connectivity of the reservoir and predict the effective direction of water injection;the mathematical model of fault-controlled carbonate reservoir with coexisting seepage and free flows can quantitatively describe the fluid flow law in the fracture-cavity body;the water injected into the fault-controlled fracture-cavity body is weakly affected by the capillary force of the lithologic body, and the oil-water movement is mainly dominated by gravity. The development modes of single well water injection, unit water injection,and single well high pressure water injection proposed based on the connection structure of fracture-cavity space and well storage space configuration are confirmed effective by pilot tests, with obvious water injection gravity flooding effect.

关键词： Tarim Basin Ordovician fault-controlled carbonate reservoir fractured-cavity carbonate reservoir water flooding gravity flooding EOR

本科生中的“科创英才”是如何“炼成”的?——情境学习理论的视角

在线全文

学校读者我要写书评

暂无评论

复旦教育论坛 2023年第5期21卷 72-82页

作者：申超姚超天津大学教育学院天津300354 浙江理工大学国际教育学院浙江杭州310018

本研究采用质性研究的范式,通过访谈法收集资料,聚焦我国工程学科领域本科生科创英才的成长过程,发现其内部存在“实验-论文”型和“竞赛-专利”型两种类型的群体分化。从情境学习的理论视角来看,前者的成长即其学习的过程通常要经过被... 详细信息

本研究采用质性研究的范式,通过访谈法收集资料,聚焦我国工程学科领域本科生科创英才的成长过程,发现其内部存在“实验-论文”型和“竞赛-专利”型两种类型的群体分化。从情境学习的理论视角来看,前者的成长即其学习的过程通常要经过被安排的“打下手”、教师主导下的课题实现及教师助力下的自主探索三个阶段,后者则常需经过依附竞赛团队的被选拔、主导竞赛团队的探索创新及引领竞赛团队走向熟练创新三个阶段。上述两类科创英才并非都严格依循以上阶段而实现逐级发展,有部分“阶段跨越者”实现了跨越式的成长。兴趣牵引下的导师与学生、学生与学生的互利共情是科创英才成长成才的主要原因。研究的教育启示在于,大学应该根据不同类型的本科生科创英才在不同发展阶段的成才特点,为其创设适宜的、支持性的学习情境,充分发挥指导教师和学生同辈群体在不同类型的本科生科创共同体中的不同作用,使本科生不仅能“进得去”,而且愿意“留下来”,进而通过与共同体内成员间的持续互动,在科创实践中逐步成长成才。

关键词：本科生拔尖创新人才工科领域情境学习理论

Structural insights of 4-Hydrophenylpyruvate dioxygenase inhibition by structurally diverse small molecules

在线全文

学校读者我要写书评

暂无评论

Advanced Agrochem

Advanced Agrochem 2022年第2期1卷 174-181页

作者： Jin Dong Jiangqing Dong Xin-He Yu yao-chao Yan Jia-Xu Nan Bo He Bao-Qin Ye Wen-chao Yang Hong-Yan Lin Guang-Fu Yang Key Laboratory of Pesticide&Chemical Biology of Ministry of Education International Joint Research Center for Intelligent Biosensor Technology and HealthCollege of ChemistryCentral China Normal UniversityWuhan430079PR China

4-Hydrophenylpyruvate dioxygenase(HPPD),a key enzyme involved in tyrosine catabolism,has long been considered a promising target for herbicides and *** types of HPPD inhibitors have been developed as high-potency drugs or *** the structural basis of the binding of these inhibitors with HPPD will be beneficial for the development of inhibitors containing novel ***,only limited structural information regarding the binding of triketone and pyrazole derivatives with HPPD has been ***,the crystal structures of HPPD complexed with inhibitors containing different scaffolds,including triketone,pyrazole,isoxazole,heterocyclic amide,and quinazolindione derivatives,were comprehensively *** binding modes of isoxazole and heterocyclic amide derivatives with HPPD were first revealed,facilitating further structural *** binding mode of compound 2 with HPPD suggests that both oxygen and nitrogen atoms can mediate coordination with the metal *** results will provide the structure-based rational design of novel HPPD inhibitors.

关键词： 4-Hydrophenylpyruvate dioxygenase(HPPD) Crystal structures Inhibitors Binding mode Herbicide