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Evaluation of the Kinetic Change of the Immunogenicity of Dengue-2 DNA Vaccine in Mice Administered by Different Administration Routes

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World Journal of Vaccines 2012年第2期2卷 61-72页

作者： Han-Chung Wu Wen-Fan Shen Gwong-Jeng J. Chang Shu-Fang Chuang Chuan-Liang Kao Day-Yu chao Division of Vector-Borne Infectious Diseases Centers for Disease Control and Prevention Fort Collins USA Graduate Institute of Microbiology and Public Health College of Veterinary Medicine National Chung-Hsing university Taichung Taiwan Institute of Medical Technology School of Medicine National Taiwan University Taipei Taiwan The Institute of Cellular and Organismic Biology Academia Sinica Taipei Taiwan

A plasmid DNA vaccine is able to induce both humoral and cellular immune responses;however, the kinetic change of the Th1/Th2 response, antibody avidity, cytokine secretion, and neutralization activity after different priming and boosting strategies have not been evaluated. A plasmid DNA, designated pCBD2 and previously shown to efficiently induce an immune response very similar to that by a wild type virus, was evaluated kinetically in this study. Our results suggest that a DNA vaccine delivered by the gene gun (gg) route produced higher and longer DENV-2-specific antibody titers than those induced through the intramuscular (im) route. Although the gg group induced a Th2 response and im delivery induced a Th1 response, priming by gg delivery, followed by a boosting by im delivery, did not shift the immune response from a Th2 to Th1 response. Furthermore, the antibody avidity (AI) measured by ELISA demonstrated a gradual increase of AI from low (AI range from 6.8% - 9.6%) on day 42 to high (AI value > 30) on day 119 in all but the gene-gun immunization group, in which an AI value of 23 was observed. Although there was lower avidity in the gg group, the mice sera from all three groups of mice demonstrated significant neutralization activity. This is the first report about the kinetics of immunogenicity of a DNA vaccine through different administration strategies, which suggests that gene gun delivery of a DNA vaccine can induce an immune response containing both neutralizing and nonneutralizing antibodies at high titers important for neutralization.

关键词： Dengue DNA Vaccine Immunological Response Gene Gun Delivery

CHANGES IN BODY WEIGHT AND ORGAN WEIGHTS OF THE HEDGEHOG IN HIBERNATION AND IN FASTING

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北京大学学报(自然科学版) 1958年第1期 117-117页

作者： Iping chao, C. J. yeh and J. S. Ch’en(Laboratory of Animal and Human Physiology,Department of Biology, Peking University, Peking)

Under the circumstances of uninterrupted deep hibernation for periods cf three and half months in open air quarters, the body weight loss of the hedgehog proceeds at a rate of 1.04 g p3r day, or 1.37 g per day per *** experimental fasting, the body weight suffers at first a rapid decrease for 1-2 days and then a more gradual diminution at a uniform rate, followed sooner or later by a terminating phase of a slow decline concurrent with a fall in body temperature to the level of the ambient temperature. The duration of fasting compatible to life varies with the initial body weight, a few days for the ordinary hedgehogs and a month or more for the exceptionally fat ones. During the period of uniform decrease, the rate of body weight loss is 11-15 g per day, slightly more in the samll animals. However, when the rate of loss is expressed in terms of g per day per kg body weight, it varies from 9 g in the very fat animals of about 1300 g to as much as 54 g in the small ones between 200 and 300 *** gene

关键词： body CHANGES IN BODY WEIGHT AND ORGAN WEIGHTS OF THE HEDGEHOG IN HIBERNATION AND IN FASTING BODY

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細菌脂多糖增强抗体形成

微生物学报 1965年第1期 35-40页

作者：楊貴貞李兴春張紹倫彭大才趙会先吉林医科大学

本实驗分为两大部分,第一部分,实驗动物为正常健康大白鼠,分别以白喉类毒素及H901伤寒菌苗加脂多糖后,一同注入动物;第二部分,健康家兔經脂多糖处理,然后于24或48小时后受400仑电离輻射,并于照射后經24、48小时进行免疫。实驗結果說明,... 详细信息

本实驗分为两大部分,第一部分,实驗动物为正常健康大白鼠,分别以白喉类毒素及H901伤寒菌苗加脂多糖后,一同注入动物;第二部分,健康家兔經脂多糖处理,然后于24或48小时后受400仑电离輻射,并于照射后經24、48小时进行免疫。实驗結果說明,細菌脂多糖与特异性抗原同时、或先24、48小时注入,可以增強血凝抗体的效价,并增加琼脂弥散反应出現的阳性率及抗原稀释倍数。电离輻射能抑制家兔抗体生成;在照射后7日左右,抗体生成的能力已完全恢复。脂多糖不但能使未經照射的正常免疫动物的抗体出現較早,效价亦較高,且亦能消除电离輻射对机体抗体产生的抑制作用,并使其效价反高于正常免疫动物,出現亦較早。可見細菌脂多糖可能是作用于抗体产生的誘导时相。可认为,脂多糖的主要作用在于对免疫器官的非特异激发,因而增強抗体的生成。

关键词：脂多糖抗体效价动物大白鼠家鼠属电离辐射电磁辐射抗体产生白喉类毒素

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荷重引发土体应力增量对压密沉陷之影响

上海国土资源 2014年第4期35卷 31-34,39页

作者：叶昭龙罗伟诚林政伟丁崇峯许时伦成功大学水工试验所地层下陷防治服务团台湾台南70955 成功大学水利及海洋工程学系台湾台南70101

本研究应用含两相非混合流体之弹性孔隙介质压密理论,考虑垂直应力增量随深度变化影响情况下,推导未饱和土壤受外加荷重之作用而产生之沉陷变化过程。模拟探讨在不同饱和度条件下孔隙水压随深度之变化及压密沉陷随时间之变化量。由模拟... 详细信息

本研究应用含两相非混合流体之弹性孔隙介质压密理论,考虑垂直应力增量随深度变化影响情况下,推导未饱和土壤受外加荷重之作用而产生之沉陷变化过程。模拟探讨在不同饱和度条件下孔隙水压随深度之变化及压密沉陷随时间之变化量。由模拟结果得知,黏土无因次孔隙水压受应力增量影响,随着深度增加而减小;且随着时间增加,无因次孔隙水压会逐渐消散至完全消散。初始饱和度较高之黏土其达完全消散后之总沉陷量较大。

关键词：孔弹性介质外加荷重压密沉陷

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TETRANDRINE的降压作用与组织胺的关系

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生理学报 1962年第1期 93-96页

作者：李德兴武汉医学院药理教研组汉口南京市南京第一医学院药理教研组

Tetrandrine(汉防己甲素)为防己科植物石蟾蜍(Stephania tetrandra ***)中所含的生物硷。已经证明该植物中所含的总生物硷有止痛作用,tetrandrine 具有消炎与抗过敏性休克作用,抗阿米巴原虫作用以及降低血压作用。在降低血压作用方面已... 详细信息

Tetrandrine(汉防己甲素)为防己科植物石蟾蜍(Stephania tetrandra ***)中所含的生物硷。已经证明该植物中所含的总生物硷有止痛作用,tetrandrine 具有消炎与抗过敏性休克作用,抗阿米巴原虫作用以及降低血压作用。在降低血压作用方面已经证明有拟胆硷神经的作用,可以取消或减弱因压迫颈动脉所引起的升压反射并且不

关键词：降压作用静脉注射盐酸苯海拉明 TETRANDRINE

Immunological Responses against Different Lineages of Influenza B Antigen in School Children during Two Consecutive Seasons

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Health 2014年第21期6卷 2837-2847页

作者： Day-Yu chao Kuang-Fu Cheng Yu-Hsin Liao Ming-Tsan Liu Ying-Hen Hsieh Tsai-Chung Li Trong-Neng Wu Chiu-Ying Chen Graduate Institute of Microbiology and Public Health College of Veterinary Medicine National Chung-Hsing University Taichung Taiwan CMU Biostatistics Center China Medical University Taichung Taiwan National Influenza Center Center for Disease Control Taipei Taiwan Graduate Institute of Biostatistics China Medical University Taichung Taiwan Department of Public Health China Medical University Taichung Taiwan

While Influenza B viruses currently circulating worldwide are of two distinct evolutionary hemagglutinin lineages, current trivalent inactivated influenza virus vaccines (TIV) contain only a single component. Single doses of TIV containing B antigen of B/Florida/4/2006 (Yamagata-like) or B/Brisbane/60/2008 (Victoria-like) were administered during 2008/2009 and 2009/2010 influenza seasons, respectively. The objective of this study was to evaluate the immunological response against different lineages of B antigens in school-aged children. A non-randomized sero-epidemiological study was conducted and the immunogenicity responses based on sero-protection rate and geometric mean titre ratio (GMTR) of hemagglutination inhibition (HI) antibodies were measured before and after immunization as well as post-influenza season. Our results suggested that school-aged children under the age of 9 years receiving TIV vaccination induced and retained higher level of sero-protection rate (66.7% and 69% for the 2008-09 and 2009-10 season, respectively) to the homologous lineage than the heterologous lineage post-vaccination (19.4% and 27.6% for the 2008-09 and 2009-10 season, respectively). The need for the quadrivalent TIV by including both lineages of influenza B viruses is recommended in this study, particularly for children under the age of 9 years.

关键词： Children Immunology Influenza Trivalent Inactivated Vaccine (TIV) Yamagata/Victoria Lineage

THE ANNULUS SIMULATION OF THE MOVEMENT OF TSINGHAI-TIBETAN HIGH AND ITS APPLICATION TO THE FORECAST OF SUMMER FLOW PATTERN OF HIGH TROPOSPHERE

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Science in China,Ser.A 1977年第5期 631-644页

作者：张可苏陈章昭周明煜李国庆宋正山王桂芳杨广基叶笃正张捷迁 ResearchGroupoftheSimulationoftheGeneralCirculation InstituteofAtmosphericPhysics AcademiaSinica

Continuing the work as reported in [1], in this article we presented the results of our experiment for the simulation of the movement of Tsinghai-Tibetan high in rotating annulus. The movement of this high may be produced in two ways. Firstly, the high itself may fluctuate in its position, but the range of this fluctuation is not large. Secondly, under the influence of the environmental circulation it moves, and it is only in this type that the large-scale movement occurs. The causes of this second type movement are as follows: (ⅰ) The intensification of the belt of westerlies may push the Tsinghai-Tibetan high southward and leads it eastward. The strengthening of the easterlies may cause it to move in an opposite way. (ⅱ) The heating downstream may induce the Tsinghai-Tibetan high to extend eastward, while the heating upstream may induce its westwardextension. (ⅲ) All the major movements of this high are accompanied with the change of the large-scale circulations. Most of its large-scale eastward mo

关键词： In high THE ANNULUS SIMULATION OF THE MOVEMENT OF TSINGHAI-TIBETAN HIGH AND ITS APPLICATION TO THE FORECAST OF SUMMER FLOW PATTERN OF HIGH TROPOSPHERE

Microglia-conditioned medium promotes locomotor recovery and neuroprotection after rat spinal cord injury

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Advances in Bioscience and Biotechnology 2012年第4期3卷 524-530页

作者： May-Jywan Tsai Pei-Jou Ho Yi-Lo Lin Ming-chao Huang Meng-Jen Lee Dann-Ying Liou Wen-Cheng Huang Yu-Show Fu Henrich Cheng Center for Neural Regeneration Neurological Institute Taipei Veterans General Hospital Taipei Taiwan Department of Anatomy and Cell Biology School of Medicine National Yang-Ming University Taipei Taiwan Department of Education and Research Taipei City Hospital Taipei Taiwan Graduate Institute of Veterinary Pathobiology College of Veterinary MedicineNational Chung Hsing University Taichung Taiwan Neural Regeneration Laboratory Neurological Institute Taipei Veterans General Hospital Taipei Taiwan

This work examines whether microglia-conditioned medium (MCM) is beneficial in stressed spinal cord cells or tissues. MCM was separated into two fractions by 50 kDa molecular cut-off centrifugation. MCM not only promoted survival of neuronal and oligodendroglial cells but effectively reduced LPS stimulation in spinal cord cultures. We further utilized the NYU weight-drop device to induce contusive spinal cord injury (SCI) in rats. Immediately after dropping the impactor from a height of 25 mm onto thoracic spinal segment, MCM was intrathecally administered. At 6 weeks post-injury, SCI rats receiving MCM > 50 kDa treatment showed significant hind-limb improvement over MCM 50 kDa, of microglia was neuroprotective against spinal cord injury.

关键词： Microglia Neuroprotection Spinal Cord Anti-Inflammation

A rapid, simple, and high-throughput UPLC-MS/MS method for simultaneous determination of bioactive constituents in Salvia miltiorrhiza with positive/negative ionization switching.

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A rapid, simple, and high-throughput UPLC-MS/MS method for s...