检索结果-南通市图书馆

Effects of nitrogen and phosphorus concentrations on the bioaccumulation of polybrominated diphenyl ethers by Prorocentrum donghaiense

引用

Journal of Environmental Sciences 2013年第2期25卷 376-385页

作者： chao chai Xundong Yin Wei Ge Jinye Wang College of Resources and Environment Qingdao Agricultural University College of Life Sciences Qingdao Agricultural University College of Animal Science and Veterinary Qingdao Agricultural University

The growth, cellular total lipids, bioaccumulation amount, and bioaccumulation factors （BAFs） of 2,4,4 ′ -tribromodiphenyl ether （BDE28）, 2,2 ′ ,4,4 ′ -tetrabromodiphenyl ether （BDE47）, and 2,2 ′ ,4,4 ′ ,5-pentabromodiphenyl ether （BDE99） in a semi-continuous culture of Prorocentrum donghaiense were studied in relation to nitrate （0, 128, and 512 μmol/L） and phosphate （0, 8, and 32 μmol/L） concentrations. The BDE28, BDE47, and BDE99 content per cell under 0 μmol N/L were 3.77 × 10 6 , 3.95 × 10 6 , and 4.32 × 10 6 ng/cell, respectively, which were significantly higher than those under 128 and 512 μmol N/L. A nearly 5-fold increase in polybrominated diphenyl ether （PBDE） content per algal cell was found between 0 and 8 μmol P/L and between 8 and 32 μmol P/L. With increasing N and P concentrations, the PBDE content per volume of algal culture and the accumulation percentage of available PBDEs declined slightly. The BAFs for the PBDEs based on lipids showed that the logBAF lip under 0 μmol N/L was higher than those under 128 and 512 μmol N/L. The logBAF lip under 0 μmol P/L was higher than that under 8 μmol P/L but lower than that under 32 μmol P/L. Correlation analysis indicated a significant negative correlation between nutrient concentration and cellular total lipids, as well as the PBDE content per cell. The results indicate that different N and P concentrations change the total lipids content of P. donghaiense, thereby resulting in varying PBDE accumulation.

关键词： nitrogen phosphorus bioaccumulation polybrominated diphenyl ethers Prorocentrum donghaiense

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Variation of Bioaccumulation Ability of 2,2',4,4'-Tetrabromodiphenyl Ether by Marine Diatom Skeletonema costatum Under Different N:P Ratios

引用

Journal of Ocean University of China 2014年第3期13卷 523-530页

作者： chai chao GE Wei YIN Xundong College of Resources and Environment Qingdao Agricultural University College of Life Sciences Qingdao Agricultural University

The growth, biochemical content and bioaccumulation quantity of 2,2',4,4'-tetrabromodiphenyl ether(BDE-47) in Skeletonema costatum were studied under different N:P ratios(1, 4, 16, 64 and 128). All cellular biochemical contents of S. costatum presented decreasing trend over cultivation time. At early stage of cultivation, the cellular protein, carbohydrate and lipid in S. costatum presented higher values in treatments of N:P=4 and 16. However, they were lower in these treatments at the late stage, but higher in treatments N:P=1 and 128. Similarly, BDE-47 levels per cell of S. costatum were higher in treatments of N:P=4 and 16 at early stage of cultivation, which were 3.8 and 3.7 ng(106 cells)-1, respectively. At the middle stage of cultivation, the BDE-47 level per S. costatum cell lowered; and it further reduced in the treatments of N:P=4 and 16 at the late stage with the values 0.6 and 0.5 ng(106 cells)-1, respectively. However, it rose in N:P=128, reaching up to 2.3 ng(106 cells)-1. Compared with BDE-47 per cell, BDE-47 per algal volume under different N:P ratios did not present obvious difference. The quantity BDE-47 accumulated per cell of S. costatum was positively correlated with protein, carbohydrate and lipid per cell; meanwhile, the BDE-47 per volume had a positive correlation with biochemical content per volume. The variation of bioaccumulation ability of BDE-47 in S. costatum can be explained by biochemical changes due to N:P ratios.

关键词： N:P ratio bioaccumulation polybrominated diphenyl ethers microalgae nutrient

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Molecular characterization and functional analysis of a piscidin gene in large yellow croaker (Larimichthys crocea)

引用

Zoological Research 2016年第6期37卷 347-355页

作者： Jing YANG Xin-Jiang LU Fang-chao chai Jiong CHEN Laboratory of Biochemistry and Molecular Biology School of Marine SciencesNingbo University Collaborative Innovation Center for Zhejiang Marine High-Efficiency and Healthy Aquaculture Ningbo University

The piscidin family, which includes potent antimicrobial peptides with broad-spectrum activity, plays an important role in the innate immune system of fish. In this study, we cloned piscidin-5-1ike type 3 （Lcpis51t3） in large yellow croaker （Larimichthys crocea）. Multiple alignments with other known piscidins revealed amino acid conservation throughout the fish, especially at the signal peptide （22 amino acids）. The phylogenetic tree confirmed that Lcpis51t3 and large yellow croaker piscidin-5-1ike proteins were grouped together to form a branch. Quantitative real-time PCR revealed that Lcpis51t3 was expressed in a wide range of tissues, including the brain, muscle, gill, head kidney, intestine, kidney, liver, and spleen. The highest mRNA expression level of Lcpis51t3 was found in the spleen. After Vibrio alginolyticus infection, mRNA expression was rapidly upregulated in the liver, head kidney, gill, kidney, and intestine at 4, 8, 12, and 24 h post infection （hpi）, whereas there were no significant changes in the spleen. The antimicrobial spectrum showed that the synthetic mature peptide of Lcpis51t3 exhibited different activity in vitro against various bacteria, such as Aeromonas hydrophila, V. anguillarum, V. alginolyticus, V. parahaemolyticus, Staphylococcus aureus, and Listeria monocytogenes In addition, survival rates from the in vivo assay indicated that the synthetic peptide of Lcpis51t3 increased the survival rate of large yellow croaker after V. alginolyticus challenge, resulting in a decline in bacterial burden and mRNA expression levels of interleukin-ll3, interleukin-10, and tumor necrosis factor-（]. These data suggest that Lcpis51t3 plays an important role in innate immunity in large yellow croaker and might represent a potential therapeutic agent against pathogen invasion.

关键词： Antimicrobial activity Large yellowcroaker Piscidin Survival rate Vibrio alginolyticus

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Ultrasmall Nanoparticles Diluted chain Entanglement in Polymer Nanocomposites

引用

Chinese Journal of Polymer Science 2019年第8期37卷 797-805页

作者： Sheng-chao chai Tian-Yang Xu Xiao Cao Gang Wang Quan Chen Hao-Long Li State Key Laboratory of Supramolecular Structure and Materials College of Chemistry Jilin University Changchun 130012 China State Key Laboratory of Polymer Physics and Chemistry Changchun Institute of Applied Chemistry Chinese Academy of Sciences Changchun 130022 China

Nanoparticle-polymer composites exhibit unusual mechanical properties and chain dynamics when the nanoparticle size is smaller than the entanglement mesh size of the matrix polymer chains, corresponding to the ultrasmall regime defined by de ***, the mechanism is still ambiguous due to the lack of suitable model systems. Here, we develop an ultrasmall nanoparticle system by using a bimodal grafting strategy to graft both short alkyl chains and long polystyrene chains onto the polyoxometalate molecular nanoparticles with a tunable repulsive potential between the nanoparticles, thus facilitating their uniform dispersion in polystyrene matrices. Linear viscoelasticity of the resultant nanocomposites changes with increasing the filler content, which shows a decrease in both plateau modulus and terminal relaxation time, indicative of a dilution effect of the nanoparticles. Namely, the entanglement network becomes sparser with increasing the filler content.

关键词： Polymer nanocomposites Ultrasmall nanoparticles chain entanglement Polyoxometalates

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Temporal and Spatial Variations of Abundance of Phycocyaninand Phycoerythrin-Rich Synechococcus in Pearl River Estuary and Adjacent Coastal Area

引用

Journal of Ocean University of China 2016年第5期15卷 897-904页

作者： JIANG Tao chai chao WANG Jifang ZHANG Ling CEN Jingyi LU Songhui Key Laboratory of Sustainable Development of Marine Fisheries of Ministry of Agriculture Yellow Sea Fisheries Research Institute Chinese Academy of Fishery Sciences Qingdao Engineering Research Center for Rural Environment Qingdao Agricultural University College of Life Science and Technology Jinan University

Three surveys were carried out in Pearl River Estuary and adjacent coastal area in May, August, and November, 2013, to investigate the temporal and spatial variations of abundance of phycoerythrin-rich Synechococcus(PE-rich SYN) and phycocyanin-rich Synechococcus(PC-rich SYN). The effects of environmental factors on the alternation of the different Synechococcus groups were also elucidated. PE-rich SYN was detected in three surveys, whereas PC-rich SYN was detected in May and August, but not in November. The highest abundances of PE-rich SYN and PC-rich SYN were recorded in August and May, with mean values of 74.17×103 and 189.92×103 cells m L-1, respectively. From May to November, the relative abundance of PE-rich SYN increased, whereas that of PC-rich SYN declined. PE-rich and PC-rich SYN presented similar horizontal distributions with high abundance in the southern estuary in May, and in the western estuary in August. The abundances of PE-rich and PC-rich SYN were high at 27–32℃and salinity of 10–20. PC-rich SYN was not detected at < 24℃, and PC:PE-rich SYN decreased in abundance with salinity increase. When less than 20 mg L-1, suspended particulate matter(SPM) was helpful for Synechococcus growth. PE-rich SYN decreased in abundance when the concentration of dissolved inorganic nitrogen increased in May and November, and the concentration of phosphate increased in November. However, PC-rich SYN abundance and nutrients showed no correlation. Principal component analysis and regression analysis indicated that PE-rich SYN significantly correlated with the principal components that were affected by environmental factors.

关键词： Pearl nutrients suspended declined dissolved abundance ammonium inorganic estuary salinity

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Selective feeding of bay scallop Argopecten irradians on phytoplankton community revealed by HPLC analysis of phytopigments in Bohai Sea, China

引用

Journal of Oceanology and Limnology 2019年第5期37卷 1746-1755页

作者： JIANG Tao WANG Longhua ZHANG Fuchong FANG Xiao LU Lin ZHANG Jihong WANG Wei QU Keming chai chao Key Laboratory of Sustainable Development of Marine Fisheries Ministry of Agriculture and Rural Aff airsYellow Sea Fisheries Research InstituteChinese Academy of Fishery SciencesQingdao 266071China Function Laboratory for Marine Fisheries Science and Food Production Processes Qingdao National Laboratory for Marine Science and TechnologyQingdao 266200China Qingdao Engineering Research Center for Rural Environment Qingdao Agricultural UniversityQingdao 266109China Ocean Fisheries Science Research Institute of Hebei Province Qinhuangdao 066201China

Understanding the feeding selectivity on phytoplankton by shellfi sh is currently a big challenge. In order to investigate the feeding behavior of bay scallop ( Argopecten irradians ) on phytoplankton, we compared its compositions of phytopigments in digestive glands with those in the surrounding seawater, and conducted fi ve consecutive investigations between July and November 2016 in a bay scallop culture area along coast of Qinghuangdao City, northwest of the Bohai Sea, China. Phytopigments in four-size fractionated phytoplankton of seawater (micro-(20-200 μm);nano(L)-[10-20 μm];nano(S)-[2.7-10 μm], and pico-[<2.7 μm]) and digestive glands of A . irradians were examined to investigate the selective feeding of A . irradians . Results show that fucoxanthin and peridinin constituted the major part of taxonomically diagnostic carotenoids (TDCs) in the micro- and nano(L)-phytoplankton in seawater. Compared with total phytoplankton biomass of seawater (TPB, sum of the four sizes), a substantial decrease of fucoxanthin proportion to total DCs in digestive glands was observed while that of peridinin, 19′-butanoyloxyfucoxanthin, alloxanthin and 19′-hexanoyloxy-fucoxanthin showed an obvious increase when those pigments were mainly confi ned to micro-sized phytoplankton (20-200 μm). However, zeaxanthin and prasinoxanthin were mainly confi ned to nano(s)- and pico-phytoplankton, of which the proportions in digestive glands were usually lower in TPB. The contribution of lutein to total DCs in digestive glands (with an average of 7.23%) increased compared with TPB of seawater (with an average of 0.63%) during all fi ve sampling times.

关键词： phytoplankton pigments selective feeding Argopecten irradians aquaculture

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Deuteration triggered downward shift of dielectric phase transition temperature in a hydrogen-bonded molecular crystal

引用

Chinese Chemical Letters 2022年第3期33卷 1422-1424页

作者： Bei-Dou Liang Tong Jin Le-Ping Miao chao-Yang chai Chang-Chun Fan Xiang-Bin Han Wen Zhang Jiangsu Key Laboratory for Science and Applications of Molecular Ferroelectrics and School of Chemistry and Chemical Engineering Southeast UniversityNanjing 211189China

Deuteration of hydrogen-bonded phase transition crystals can increase the transition temperatures due to the isotope effect. But rare examples show the opposite trend that originates from the structural changes of the hydrogen bond, known as the geometric H/D isotope effect. Herein, we report an organic crystal, diethylammonium hydrogen 1,4-terephthalate, exhibits a reversible structural phase transition and dielectric switching. Structural study shows the cations reside in channels formed by one-dimensional hydrogen-bonded anionic chains and undergo an order-disorder transition at around 206 K. The deuterated counterpart shows an elongation of the O…O hydrogen bond by about 0.005 A. This geometric isotope effect releases the internal pressure of the anionic host on the cation guests and results in a downward shift of the phase transition temperature by 10 K.

关键词： Dielectric phase transition Stimulus responsive material Deuteration Geometric isotope effect Downward shift of transition temperature

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Synthesis and biological activities of N-dichloroacetyl-2-n-propyl-1-oxa-4-aza-spiro-4, 5-decane

引用

Journal of Northeast Agricultural University(English Edition) 2007年第1期14卷 64-66页

作者： YE Fei chai chao College of Science Northeast Agricultural University Harbin 150030 China

The title compound was synthesized and characterized by IR, ^1HNMR and elemental analysis. The preliminary biological test showed that the compound protected maize against injury by imazethapyr to some extent.

关键词： safener synthesis oxazolidine biological activity

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Determination of the spatial and temporal variability of phytoplankton community structure in Daya Bay via HPLC-CHEMTAX pigment analysis

引用

Journal of Oceanology and Limnology 2018年第3期36卷 750-760页

作者： WANG Longhua OU Linjian HUANG Kaixuan chai chao WANG Zhaohui WANG Xiaomin JIANG Tao Key Laboratory of Sustainable Development of Marine Fisheries Ministry of AgricultureYellow Sea Fisheries Research InstituteChinese Academy of Fishery SeieneesQingdao 266071China Qingdao Engineering Research Center for Rural Environment Qingdao Agricultural UniversityQingdao 266109China College of Life Science and Technology Jinan UniversityGuangzhou 510632China

The spatial and temporal variability of the phytoplankton community structure in Daya Bay, South China Sea, were identified by using HPLC-CHEMTAX analytical techniques. The highest chlorophyll a(Chl a) concentrations were observed during summer(with an average value of 0.84 μg/L) and lowest ones during winter(with an average value of 0.33 μg/L). CHEMTAX processing revealed the seasonal succession of phytoplankton species in Daya Bay. During winter, diatoms were the dominant phytoplankton species and contributed 41.5% to total Chl a. Based on Chl a concentration, the average ratio of dinofl agellates to total phytoplankton biomass substantially increased with increasing temperature and nitrogen to phosphorus(N/P) ratio, reaching 52.2% in spring. Nutrient limitation shifted from phosphorus to nitrogen during summer. Moreover, this period was associated with the predominance of diatoms, which accounted for 71.1% of Chl a. Prasinophytes and cryptophytes were the other two dominant groups and particularly dominated during winter. Cyanobacteria became an important group during summer and autumn. Canonical correspondence analysis suggested that chrysophytes, dinofl agellates, and cryptophytes were strongly associated with high nitrate concentration, ammonium, dissolved inorganic nitrogen(DIN), and N/P ratio, and were negatively associated with temperature and phosphate. Diatoms and cyanobacteria were strongly associated with temperature, phosphate, and salinity, and are negatively influenced by nitrate, ammonium, DIN, and N/P ratio. Microscopic observations and pigment HPLC information were in good agreement for diatoms and dinofl agellates in the bay. This study demonstrated the usefulness of pigment analysis in investigating the distribution of phytoplankton groups in a complex physical environment, such as Daya Bay.

关键词： phytoplankton pigments environmental factor HPLC-CHEMTAX Daya Bay

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Mass Spectrometry and Mass Spectrometry Imaging-based Thyroid Cancer Analysis

引用

Journal of Analysis and Testing 2022年第3期6卷 235-246页

作者： Hao-Jiang Wang Bo Li Meng-Ting Zhang chao-Fan chai Xiao-Rong Li Ning Li Hong Xiao Wei Bian Department of Biochemistry and Molecular Biology School of Basic Medical ScienceShanxi Medical UniversityTaiyuan 030001China College of Pharmacy Shanxi Medical UniversityTaiyuan 030001China Department of Pathology First Hospital of Shanxi Medical UniversityTaiyuan 030001China

Thyroid carcinoma is one of the most common endocrine malignant diseases *** the rapid development of medical technology,early and effective diagnostic methods could be able to improve the survival rate and quality of life of patients suffering from the *** the complexity of cancer,some specific detection method is desired for diagnosis and *** spectrometry imaging(MSI)is an emerging technique for acquiring molecular information from biological tissues without staining and labeling,including qualitative,quantitative and spatial distribution *** the past several decades,MSI has been widely used for pharmacological monitoring,biomolecular imaging of cells and *** this review,we introduce the tumor progression and histological characteristics of thyroid cancer,and focus mainly on the preparation of biological specimens for MSI and mass spectrometry(MS)analysis,as well as the recent progress in MS and MSI-based thyroid cancer *** review thoroughly discusses the importance of MS and MSI for clinical diagnosis,identification and prognosis of thyroid cancer,and provides some new clues for molecular mechanisms research and tumor metastasis.

关键词： Thyroid cancer Metabolic heterogeneity Mass spectrometry imaging Mass spectrometry Tumor biomarker

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：