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Stress responsive gene CIpK14 is involved in phytochrome A-mediated far-red light inhibition of greening in Arabidopsis

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science china(Life sciences) 2010年第11期53卷 1307-1314页

作者： QIN YuZhi2,3, GUO Ming1, LI Xu1, XIONG XingYao2,3, HE ChangZheng2,3, NIE XianZhou4 & LIU XuanMing1 1College of Life science and Biotechnology, Hunan University, changsha 410082, china 2College of Horticulture and Landscape, Hunan Agricultural University, changsha 410128, china 3Hunan provincial key laboratory of Crop Germplasm Innovation and Utilization, Hunan Agricultural University, changsha 410128, china 4potato research Centre, Agriculture and Agri-food Canada, Fredericton, New Brunswick E3B 4Z7, Canada 2. College of Horticulture and Landscape Hunan Agricultural University Changsha 410128 China3. Hunan Provincial Key Laboratory of Crop Germplasm Innovation and Utilization Hunan Agricultural University Changsha 410128 China1. College of Life Science and Biotechnology Hunan University Changsha 410082 China4. Potato Research Centre Agriculture and Agri-Food Canada Fredericton New Brunswick E3B 4Z7 Canada

In this study, we show that CIpK14,a stress responsive CBL-interacting protein kinase gene,is involved in phytochrome A-mediated far-red light inhibition of greening in Arabidopsis seedlings. The CIpK14-impairment mutant cipk14 grown in continuous far-red (Fr) light did not show greening when exposed to white light illumination for 15 h. By contrast, the Fr-grown phytochrome A null mutant phyA greened within 0.5 h of exposure to white light. Although greening of Col-4 (wild-type) was not completely abolished by Fr, it exhibited a significantly decreased greening capacity compared with that of phyA. Further analyses demonstrated that the expression of protochlorophyllide reductase (pOr) genes was correlated with the greening ability of the genotypes. In addition, CIpK14 appeared to be regulated by both the circadian clock and phyA. Taken together, these results suggest that CIpK14 plays a role in phyA-mediated Fr inhibition of seedling greening, and that a Ca-related kinase may be involved in a previously undefined branch point in the phytochrome A signaling pathway.

关键词： chlorophyll CIpK14 far-red light inhibition of greening pOr phytochrome A

Effects of Lactosucrose on intestinal barrier in TNBS-lnduced Colitis in rats

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Effects of Lactosucrose on intestinal barrier in TNBS-lnduce...

生猪优质健康养殖关键技术国际学术研讨会

作者： Z ruan~1 YLYin~(*1 2) GY Wu~2 (State key laboratory of food science and technology Nanchang University Nanchang 330047 china Institute of Subtropical Agriculture the Chinese Academy of sciences changsha 410125 china)

＜正＞To investigate the effects of Lactosucrose on intestinal barrier in TNBS-Induced *** Lactosucrose were administered rats with experimental ulcerative colitis induced by 2,4,6-trinitrobenzene sulfonic acid（TNBS）.The colon structure was observed hematoxylin-eosin ***,a kind of tight junction protein,were determined by immunohistochemical *** mrNA expression was assessed by reverse transcriptase-polymerase chain reaction（rT-pCr）.results Lactosucrose significantly improved

关键词： Lactosucrose rats

A Novel Kernel Fisher Discriminant Analysis:Constructing Informative Kernel by Decision Tree Ensemble for Metabolomics Data Analysis

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A Novel Kernel Fisher Discriminant Analysis:Constructing Inf...

中国化学会第28届学术年会

作者： Dong-Sheng Cao1 Mao-Mao Zeng2 Jun Yan1 Jian-Hua Huang1 Qian-Nan Hu4 Qing-Song Xu3 Yi-Zeng Liang*1 1research center of modernization of traditional Chinese medicines Central South University changsha 410083.p.r.china 2.tate key laboratory of food science and technology School of food science and technology Jiangnan Univesity Wuxi 214122 p.r.china 3School of Mathematical sciences and Computing technology Central South University changsha 410083.p.r.china 4key laboratory of Combinatorial Biosynthesis and Drug Discovery (Wuhan University) Ministry of Education and Wuhan University School of pharmaceutical sciences Wuhan 430071 p.r.china research center of modernization of traditional Chinese medicines Central South UniversityChangsha 410083 P. R. China State Key Laboratory of Food Science and Technology School of Food Science and Technology Jiangnan Univesity Wuxi 214122 P.R.China Research center of modernization of traditional Chinese medicines Central South UniversityChangsha 410083 P.R. China Key Laboratory of Combinatorial Biosynthesis and Drug Discovery(Wuhan University)Ministry of Education and Wuhan University School of Pharmaceutical SciencesWuhan 430071 P.R.China School of Mathematical Sciences and Computing Technology Central South UniversityChangsha 410083 P.R.China

Large amounts of data from high-throughput metabolomics experiments become commonly more and more complex,which brings an enormous amount of challenges to existing statistical *** there is a need to develop statistically efficient approach for mining the underlying metabolite information contained by metabolomics data under *** the work,we developed a novel kernel Fisher discriminant analysis （KFDA） algorithm by constructing an informative kernel based on decision tree *** constructed kernel can effectively encode the similarities of metabolomics samples between informative metabolites/biomarkers in specific parts of the measurement ***,informative metabolites or potential biomarkers can be successfully discovered by variable importance ranking in the process of building ***,KFDA can also deal with nonlinear relationship in the metabolomics data by such a kernel to some ***,two real metabolomics datasets together with a simulated data were used to demonstrate the performance of the proposed approach through the comparison of different approaches.

关键词： metabolomics kernel methods Fisher discriminant analysis (FDA) biomarker discovery decision tree classification and regression tree (CArT)

proteomic analysis of pollens from Honglian cytoplasmic male sterility rice

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Proteomic analysis of pollens from Honglian cytoplasmic male...

第二届全国植物蛋白质组学学术研讨会

作者： Li Wen~(1,2) Gai Liu~2 Shaoqing Li~2 Cuixiang Wan~2 Jun Tao~1 Kaiyang Xu~1 Guomin Li~1 Yingguo Zhu~2 1 School of Biological and food Engineering,changsha University of science and technology,changsha,Hunan 410076,p.r.china. 2 key laboratory of MOE for plant Developmental Biology,College of Life sciences,Wuhan University,Wuhan,Hubei 430072,p.r.china.

Cytoplasmic male sterility (CMS),which eliminates the possibility of self-pollination,is a commonly observed phenomenon in the plant kingdom and has been exploited for hybrid seed *** (Oryza sativa L.) is one of the m... 详细信息

关键词： Oryza sativa L pollen Cytoplasmic male sterility Two-dimensional electrophoresis Mass spectrometry.

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腊肉真空腌制工艺条件的优化

食品科技 2011年第7期36卷 116-119页

作者：刘成国罗扬王冬冬陈瑶湖南农业大学食品科学技术学院长沙410128 食品科学与生物技术湖南省重点实验室长沙410128

以猪脊背肉为原料,研究了真空腌制工艺中真空压力、腌制液浓度、原料质量和真空腌制时间4个因素对其真空腌制速率的影响。单因素实验及正交实验结果表明,腊肉真空腌制的最佳条件为:真空压力86kpa、腌制液浓度25%、原料质量125g、真空腌... 详细信息

以猪脊背肉为原料,研究了真空腌制工艺中真空压力、腌制液浓度、原料质量和真空腌制时间4个因素对其真空腌制速率的影响。单因素实验及正交实验结果表明,腊肉真空腌制的最佳条件为:真空压力86kpa、腌制液浓度25%、原料质量125g、真空腌制时间5h,方差分析结果显示该最佳工艺与其他各组工艺存在着极显著差异。

关键词：真空腌制猪肉腊肉

Effects of low temperature on survival and fecundity of Alternanthera philoxeroides Griseb

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Effects of low temperature on survival and fecundity of Alte...

第二届全国生物入侵学术研讨会

作者： Wan Fanghao (1 State key laboratory for Biology of plant Diseases and Insect pests,Institute of plant protection, Chinese Academy of Agricultural science,Beijing 100094 2 Central South University of Forestry and technology,changsha 412000 3 Institute of plant protection,Hunan Academy of Agricultural science,changsha 412002)

[Objective]The paper studied about the effects of low temperature on survival and fecundity of Alternanthera philoxeroides Griseb.[Method]The egg hatching rate,larval fecundity and feed,the fecundity,lifetime and feed of the adult were exposed to 4,6,8,10and 12℃for different time.[result]The hatching rate of egg were significant differences between 4 and 10℃for 1 day,but no between 10 and 12℃;and There were significant differences between 4,6℃and 10,12℃for 4 days;and hatching rate were sharply cut down at 4,6,8℃for 7 *** larval and the adult did not feed anything at 4℃,and got more food as the temperature increased,however,the larval could not complete theri development within 4 and 12℃,and adult did not lay any eggs within 4 and 10℃and did a few at 12℃.The deaths peak of adult were apparent by 6-8 days after exposureing within 4 and 12℃.[Conclusion]The low temperature have a significant effect on egg hatching rate,larval fecun dity,and adult laying egg,and have a serious effect on overwinter of Alternanthera philoxeroides Griseb.

关键词： Alternanthera philoxeroides Griseb Low temperature Survival Fecundity

Effects of lactosucrose on intestinal barrier in tnbs-induced colitis in rats

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Effects of lactosucrose on intestinal barrier in tnbs-induce...

生猪优质健康养殖关键技术国际学术研讨会

作者： ruan Zheng~1 Huang Xiaoliu~1 WuXin~(1 2) Zhou Yan~1 WU Guo-yao~3 Yulong Yin~(1 2*) (1.State key laboratory of food science and technology Nanchang University Nanchang 330047 china 2.research Center for Healthy Breeding of Livestock and poultry Institute of Subtropical Agriculture the Chinese Academy of sciences changsha 410125 china 3.College of Agriculture and Life sciences Texas A&M University College Station 77843-2471 USA)

＜正＞Aims To investigate the effects of Lactosucrose on intestinal barrier in TNBS-Induced *** Lactosucrose were administered rats with experimental ulcerative colitis induced by 2,4,6-trinitrobenzene sulfonic acid（TNBS）.The colon structure was observed hematoxylin-eosin ***,a kind of tight junction protein,were determined by immunohistochemical *** mrNA expression was assessed

关键词： Lactosucrose Intestinal barrier Occludin Colitis

random Fuzzy Chance-constrained programming Based on Adaptive Chaos Quantum Honey Bee Algorithm and robustness Analysis

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International Journal of Automation and computing 2010年第1期7卷 115-122页

作者： Han Xue Xun Li Hong-Xu Ma College of Electromechanical Engineering and Automation, National University of Defense technology, changsha 410073, prC College of Electromechanical Engineering and Automation National University of Defense Technology Changsha PRC

This paper proposes an adaptive chaos quantum honey bee algorithm （CQHBA） for solving chance-constrained program- ming in random fuzzy environment based on random fuzzy simulations. random fuzzy simulation is designed to estimate the chance of a random fuzzy event and the optimistic value to a random fuzzy variable. In CQHBA, each bee carries a group of quantum bits representing a solution. Chaos optimization searches space around the selected best-so-far food source. In the marriage process, random interferential discrete quantum crossover is done between selected drones and the queen. Gaussian quantum mutation is used to keep the diversity of whole population. New methods of computing quantum rotation angles are designed based on grads. A proof of con- vergence for CQHBA is developed and a theoretical analysis of the computational overhead for the algorithm is presented. Numerical examples are presented to demonstrate its superiority in robustness and stability, efficiency of computational complexity, success rate, and accuracy of solution quality. CQHBA is manifested to be highly robust under various conditions and capable of handling most random fuzzy programmings with any parameter settings, variable initializations, system tolerance and confidence level, perturbations, and noises.

关键词： Honey bee algorithm random fuzzy programming quantum computation chaos optimization robustness

Effects of Lactosucrose on Intestinal Barrier in TNBS-induced Colitis in rats

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Effects of Lactosucrose on Intestinal Barrier in TNBS-induce...

中国畜牧兽医学会动物营养学分会第十一次全国动物营养学术研讨会

作者： Z.ruan~1 X.Huang~1 X.Wu~(1,2) Y.Zhou~1 G.WU~3 Y.Yin~(1,2*) (1.State key laboratory of food science and technology,Nanchang University,Nanchang 330047,china 2.research Center for Healthy Breeding of Livestock and poultry,Hunan Engineering and research Center of Animal and poultry science,Institute of Subtropical Agriculture,the Chinese Academy of sciences, changsha 410125,china 3.College of Agriculture and Life sciences,Texas A&M University,College Station 77843-2471,USA)

Aims To investigate the effects of Lactosucrose on intestinal barrier in TNBS-Induced *** Lactosucrose were administered rats with experimental ulcerative colitis induced by 2,4,6-trinitrobenzene sulfonic acid(TNBS).The colon structure was observed hematoxylin-eosin ***,a kind of tight junction protein,were determined by immunohistochemical *** mrNA expression was assessed by reverse transcriptase-polymerase chain reaction(rT-pCr).results Lactosucrose significantly improved changes in the histological inflammation score as compared with TNBS-induced *** protein content of occludin and its mrNA expression were increased compared with TNBS-induced *** These results suggest that Lactosucrose can recover mucosal injury due to increase of occludin in TNBS-induced colitis.

关键词： Lactosucrose Intestinal barrier Oceludin Colitis