检索结果-南通市图书馆

基于柯氏评估模型分析中医学本科实习生中医临床思维效果研究

在线全文

学校读者我要写书评

暂无评论

中国中医药现代远程教育 2024年第14期22卷 1-3页

作者：赖长沙吴雁福建中医药大学附属人民医院传统内科福建福州350004 福建中医药大学附属人民医院教务科福建福州350004

目的探讨应用柯氏评估模型评估中医学本科实习生中医临床思维培训的效果。方法选取福建中医药大学附属人民医院传统内科中医学本科实习生40人,随机分为对照组和实验组,各20人。对照组以传统模式培训,实验组采用“中医辨治六步程式”逐... 详细信息

目的探讨应用柯氏评估模型评估中医学本科实习生中医临床思维培训的效果。方法选取福建中医药大学附属人民医院传统内科中医学本科实习生40人,随机分为对照组和实验组,各20人。对照组以传统模式培训,实验组采用“中医辨治六步程式”逐步强化的培训模式。培训2周后,应用柯氏评估模型综合评估培训的效果。结果反应层:对比两组对培训的满意度,差异有显著统计学意义(P<0.01)。学习层:比较两组培训后理论、中医临床思维与操作能力考核成绩,差异有显著统计学意义(P<0.01)。行为层:培训后比较医生对两组在实践中运用中医临床思维与操作技能的满意度,差异有显著统计学意义(P<0.01)。结果层:比较经管患者的满意度,两组差异有显著统计学意义(P<0.01);两组投诉率、事故率均为0。结论柯氏评估模型可从整体上评估中医本科实习生临床培训的效果,科学性与可行性强;“中医辨治六步程式”逐步强化的培训模式可明显提高本科实习生中医临床思维与诊疗能力。

关键词：柯氏评估模型实习生中医临床思维临床教学

2007 changsha International Symposium on Lupus

在线全文

学校读者我要写书评

暂无评论

Chinese Medical Journal 2007年第16期120卷 1471-1472页

作者： LU Qian-jin LI Ya-ping Institute of Dermatovenereology Epigenetic Research Center Central South University changsha 410011,China Institute of Dermatovenereology Epigenetic Research Center Central South University changsha 410011 China）

The 2007 changsha International Symposium on Lupus, co-sponsored by the Institute of Dermatovenereology and the Epigenetic Research Center of the Second Xiangya Hospital at the Central South University was successfully held in changsha on May 27, 2007. The symposium, having attracted over 500 experts from China, USA, France and Japan, was presided by Dr. LU Qian-jin, Director of the Institute of Dermatovenereology at the Central South University, China. Special lectures were given respectively by Dr. Bruce Richardson from the University of Michigan, USA, Dr. ZHENG Jie from the Shanghai Jiaotong University Medical School, China, Dr. Pierre Youinou from the Brest University Medical School.

关键词： SLE changsha International Symposium on Lupus gene MRL

Vitrectomy for vitreous amyloidosis

在线全文

学校读者我要写书评

暂无评论

International Journal of Ophthalmology(English edition) 2011年第3期4卷 307-310页

作者： Jiang You Department of Ophthalmology,the Second Xiangya Hospital of Central South University,changsha 410011,Hunan Province,China Department of Ophthalmology the Second Xiangya Hospital of Central South University Changsha 410011 Hunan Province China.

AIM: To study the clinical features of vitreous amyloidosis and the effect of vitrectomy for it. METHODS: We treated 6 eyes (4 Patients) with vitreous amyloidosis from 2008 to 2009, and followed up for 18-30 months after vitrectomy. RESULTS: The visual acuity ranged from counting fingers to 3/50 before surgery and 15/50-40/50 after vitrectomy. No severe complication was observed. CONCLUSION: Vitrectomy is an effective and safe treatment for eyes with vitreous amyloidosis. A long period of follow-up after surgery should be performed.

关键词： vitreous amyloidosis surgical treatment

Sequencing of phosphopeptides sulfonated by 4-sulfophenyl isothiocyanate using post-source decay matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Progress in Natural Science:Materials International 2006年第9期16卷 930-935页

作者： TANG Jianguang , WANG Yongjun , ZHANG Hainan, WANG Chunyu, HU Zhiping, XUE Zhigang, XIA Kun3 and SHI Xiaoliu ( Department of Neurology, the Second Xiangya Hospital, Central South University, changsha 410011, China Department of Digestion, the Second Xiangya Hospital, Central South University, changsha 410011, China National Key Laboratory of Chinese Genetics, changsha 410078, China) Department of Neurology The Second Xiangya Hospital Central South University Changsha 410011 China Department of Digestion The Second Xiangya Hospital Central South University Changsha 410011 China National Key Laboratory of Chinese Genetics Changsha 410078 China

Phosphorylation/dephosphorylation is probably the most common and important reversible post-translational modification of proteins. Analyzing the functional effects of phosphorylation is helpful for understanding the biological functions of proteins. Identification of the phosphorylation sites of phosphorylated protein is a prerequisite for research on phosphorylation. In this work, an effective and simple method of identification of protein phosphorylation sites has been developed. Phosphopeptides were selectively enriched with immobilized metal affinity chromatography (IMAC) and subsequently chemically modified by 4-sulfophenyI isothiocyanate, and then the chemically modified phosphopeptides were sequenced with post-source decay (PSD) matrix-assisted laser desorption/ionization (MALDI) time-of-flight mass spectrometry for detecting phosphorylation sites. The charge of derivatization by 4-sulfophenyl isothiocyanate introduces a negative sulfonic acid group at the N-terminus of a peptide, and enables the selective detection of only a single series of C-terminal y-type ions. This chemically assisted method greatly simplifies the extremely complex pattern of PSD fragment ions and makes the PSD spectra more easier to be interpreted. The phosphorylation sites of a synthesized model phosphopeptide and human c-myc protein have been successfully identified by this method.

关键词： phosphopeptides sequencing 4-sulfophenyl isothiocyanate post-source decay matrix-assisted laser desorption/ion-ization time-of-flight mass spectrometry.

Surgical Treatment for Complications of Congenital Retinoschisis

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Journal of Huazhong University of Science and Technology(Medical Sciences) 2011年第3期31卷 404-408页

作者：游江 Department of Ophthalmology Xiangya HospitalCentral South University

This study examined the clinical features of complications of congenital retinoschisis and the clinical efficacy of vitreoretinal surgery in the treatment of these complications.The clinical efficacy of surgical treatments was retrospectively analyzed in 10 patients with congenital retinoschisis（10 eyes） complicated with rhegmatogenous retinal detachment（n=5）,vitreous hemorrhage（n=2） and macula-involving schisis（n=1）.All the patients suffered foveal and peripheral schisis.They were treated with scleral buckling（n=1） or vitrectomy（n=9）.After the surgical treatment,the retina was reattached in patients with rhegmatogenous retinal detachment;the refractive media became transparent in those with vitreous hemorrhage;the visual acuity in 80% of patients was improved;no remarkable progression of schisis was found;no severe operative complications occurred.It was concluded that vitreoretinal surgery in the treatment of complications of congenital retinoschisis is safe and effective,and helps improve and maintain the visual function.

关键词： congenital retinoschisis complication vitreoretinal surgery

在线全文

学校读者我要写书评

暂无评论

青冈栎原花青素合成基因的鉴定与分析

中南林业科技大学学报 2024年第5期44卷 167-180页

作者：曹睿彬杨俊杰罗常莎姜小龙徐刚标中南林业科技大学林木遗传育种实验室湖南长沙410004 湖南省青羊湖国有林场湖南宁乡410600

【目的】对青冈栎原花青素(缩合单宁)合成基因进行鉴定及生物信息学分析,可为青冈栎果实资源开发利用和品种改良奠定分子基础。【方法】基于已公布的青冈栎全基因组测序数据,利用生物信息学方法对原花青素合成基因进行鉴定,并分析其蛋... 详细信息

【目的】对青冈栎原花青素(缩合单宁)合成基因进行鉴定及生物信息学分析,可为青冈栎果实资源开发利用和品种改良奠定分子基础。【方法】基于已公布的青冈栎全基因组测序数据,利用生物信息学方法对原花青素合成基因进行鉴定,并分析其蛋白质序列、染色体定位、种间共线性、基因结构及系统发育关系。【结果】青冈栎中11种原花青素合成基因有42个家族成员,分布在11条染色体上;蛋白质理化性质在具有多拷贝数的QgPAL、QgC4H、Qg4CL、QgF3′H基因家族成员间存在差异;Qg4CL8、Qg4CL14、Qg4CL17、QgF3’H2、QgC4H4蛋白具有2~3个跨膜区域,表明这些蛋白可能在细胞内外信号转导中发挥重要作用;基因家族成员在栎属中进化比较保守,通过栎属古老的全基因组复制及串联重复和片段重复扩张;QgPAL2~QgPAL6和Qg4CL15基因分别与拟南芥中参与原花青素合成的AtPAL1~2和At4CL3聚为同一分支,可能在原花青素生物合成中发挥重要作用;Qg4CL基因家族在系统发育树中进化出新的分支,其家族成员表现出基因结构的多样性,并在4CL基因的高度保守基序中发生氨基酸位点的突变,推测这些基因可能因进化出新功能而被保留下来。【结论】青冈栎原花青素合成基因通过多种复制方式进行扩张,复制基因在栎属中相对保守,在青冈栎中表现出理化性质、蛋白结构和进化模式的多样化。采用生物信息学方法分析了原花青素合成基因的特征和进化模式,这为深入解析栎属植物单宁合成机制奠定了分子基础。

关键词：青冈栎原花青素合成基因生物信息学进化特征

在线全文

学校读者我要写书评

暂无评论

无症可辨视域下中医诊断思维应用策略述要

中医杂志 2024年第7期65卷 669-673页

作者：陈斌斌王洋唐文王志斌赖长沙李灿东福建中医药大学中医证研究基地福建省福州市350122 福建中医药大学附属人民医院

无症可辨的实质是疾病处于特殊阶段、特殊状态,无症可辨时如何进行中医诊断是当前中医诊疗的关键科学问题。造成无症可辨的原因包括医患行为与医患关系的局限以及思维认知的局限。为应对此类情况,可以从思维层面采取以下策略:基于整体... 详细信息

无症可辨的实质是疾病处于特殊阶段、特殊状态,无症可辨时如何进行中医诊断是当前中医诊疗的关键科学问题。造成无症可辨的原因包括医患行为与医患关系的局限以及思维认知的局限。为应对此类情况,可以从思维层面采取以下策略:基于整体观念把握疾病的发生发展及内在机制、运用辩证思维总结疾病规律、临床实践与经验思维相结合、多学科交叉引入新思维模式。这些策略有助于中医医师在无明显症状的情况下进行准确诊断,为患者提供更好的医疗服务。

关键词：中医诊断辨证中医思维无症状

Activating transcription factor 3 is up-regulated in sporadic cases of isolated hypospadias

在线全文

学校读者我要写书评

暂无评论

Activating transcription factor 3 is up-regulated in sporadi...

Paclitaxel ameliorates fibrosis in hepatic stellate cells via inhibition of TGF-β/Smad activity

第十三届全国泌尿外科学术会议暨第八届全球华人泌尿外科学...

作者： LI yuanwei ZHAO xiaokun. Department of Urology The Second Xiangya Hospital Central South University changsha 410011 China.

＜正＞Background: Activating transcription factor 3 （ATF3） is a member of the ATF/CREB （cAMP responsive element binding） family of transcription factors. It is almost certain that ATF3 protein regulates a variety ... 详细信息

关键词： isolated hypospadias sporadic ATF3 RT-PCR IHC

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2010年第26期16卷 3330-3334页

作者： Jun Zhou, De-Wu Zhong, Qun-Wei Wang, Xiong-Ying Miao, Xun-Di Xu, Department of Hepatobiliary Surgery, Second Xiangya Hospital, Central South University, changsha 410011, Hunan Province, China Department of Hepatobiliary Surgery Second Xiangya Hospital Central South University Changsha 410011 Hunan Province China.

AIM: To investigated if paclitaxel can attenuate hepatic fi brosis in rat hepatic stellate cells (RHSCs). METHODS: RHSCs were cultured in vitro and randomly assigned to four groups: normal control group (treated only with Dulbecco's Modified Eagle's Medium), Taxol group (200 nmol/L paclitaxel was added to the cell culture), transforming growth factor (TGF)-β group (5 ng/mL recombinant human TGF-β1 was added to the cell culture), and TGF-β + Taxol group. TGF-β signaling cascade and status of various extracellular matrix proteins were evaluated by real time reverse transcriptase polymerase chain reaction and Western blotting. RESULTS: The paclitaxel treatment markedly suppressed Smad2/3 phosphorylation. This was associated with attenuated expression of collagen Ⅰ and Ⅲ and fi bronectin in RHSCs.CONCLUSION: These data indicate that 200 nmol/L paclitaxel ameliorates hepatic fi brosis via modulating TGF-β signaling, and that paclitaxel may have some therapeutic value in humans with hepatic fi brosis.

关键词： Transforming growth factor-β Hepatic fibrosis Paclitaxel Smad Microtubules