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Effects of dietary Clostridium butyricum and rumen protected fat on meat quality,oxidative stability,and chemical composition of finishing goats

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Journal of Animal Science and Biotechnology 2024年第2期15卷 911-924页

作者： Meimei Zhang Zhiyue Zhang Xinlong Zhang changming lu Wenzhu Yang Xiaolai Xie Hangshu Xin Xiaotan lu Mingbo Ni Xinyue Yang Xiaoyang Lv Peixin Jiao College of Animal Science and Technology Northeast Agricultural UniversityHarbin 150030People’s Republic of China Lethbridge Research and Development Centre Lethbridge T1J 4B1Canada School of Agriculture and Food Sciences The University of QueenslandGattonQLD 4343Australia International Joint Research Laboratory in Universities of Jiangsu Province of China for Domestic Animal Germplasm Resources and Genetic Improvement Yangzhou 225009China

Background Clostridium butyricum(CB)is a probiotic that can regulate intestinal microbial composition and improve meat *** protected fat(RPF)has been shown to increase the dietary energy density and provide essential fatty ***,it is still unknown whether dietary supplementation with CB and RPF exerts beneficial effects on growth performance and nutritional value of goat *** study aimed to investigate the effects of dietary CB and RPF supplementation on growth performance,meat quality,oxidative stability,and meat nutritional value of finishing ***-two goats(initial body weight,20.5±0.82 kg)were used in a completely randomized block design with a 2 RPF supplementation(0 vs.30 g/d)×2 CB supplementation(0 vs.1.0 g/d)factorial treatment *** experiment included a 14-d adaptation and 70-d data and sample collection *** goats were fed a diet consisted of 400 g/kg peanut seedling and 600 g/kg corn-based concentrate(dry matter basis).Result Interaction between CB and RPF was rarely observed on the variables measured,except that shear force was reduced(Plues(P=0.007),total antioxidant capacity(P=0.050),glutathione peroxidase activities(P=0.006),concentrations of 18:3(Plues(P<0.001),shear force(P=0.050)and malondialdehyde content(P=0.044)were decreased by adding ***,CB supplementation increased essential amino acid(P=0.027),flavor amino acid(P=0.010)and total amino acid contents(P=0.024)as well as upregulated the expression of lipoprotein lipase(P=0.034)and peroxisome proliferator-activated receptorγ(PPARγ)(P=0.012),and downregulated the expression of stearoyl-CoA desaturase(SCD)(P=0.034).The RPF supplementation increased dry

关键词： Chemical composition Clostridium butyricum Goats Meat quality Oxidative stability Rumen protected fat

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不同蛋白源代乳粉对羔羊生长、消化、瘤胃发酵参数和菌群结构的影响

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饲料工业 2024年第6期45卷 48-56页

作者：王紫薇梁鸽鸽鲁昌明张旭张永根谢小来扈光辉路允龙东北农业大学动物科学技术学院黑龙江哈尔滨150030 黑龙江绿能生态牧业有限公司黑龙江齐齐哈尔162400

研究旨在探究不同蛋白源代乳粉对羔羊生长、消化、瘤胃发酵参数和菌群结构的影响。选取40只健康、体重相近的3日龄萨能公羔羊,饲喂乳源蛋白代乳粉至22日龄,随机分为2组。对照组继续饲喂乳源蛋白代乳粉(milk-derived protein milk replac... 详细信息

研究旨在探究不同蛋白源代乳粉对羔羊生长、消化、瘤胃发酵参数和菌群结构的影响。选取40只健康、体重相近的3日龄萨能公羔羊,饲喂乳源蛋白代乳粉至22日龄,随机分为2组。对照组继续饲喂乳源蛋白代乳粉(milk-derived protein milk replacer,MD组),试验组逐渐替代饲喂植物源蛋白代乳粉(plant-derived protein milk replacer,PD组),试验期40 d。结果表明:①与MD组相比,PD组终末体重、平均日增重(ADG)、开食料干物质采食量和干物质采食量(DMI)显著降低(P0.05)。②与MD组相比,PD组粗蛋白(CP)、粗脂肪(EE)、钙(Ca)和磷(P)的表观消化率均显著降低(P<0.05)。③与MD组相比,PD组瘤胃中氨态氮(NH3-N)、异戊酸浓度显著提高(P<0.05);④PD组瘤胃Chao1指数、操作分类单位(OTU)数量、香农(Shannon)指数、均匀性(Evenness)指数以及辛普森(Simpson)指数均显著低于MD组(P<0.05);主坐标分析(PCoA)显示,两组羔羊瘤胃微生物间结构差异显著(P<0.05);在门水平上,与MD组相比,PD组厚壁菌门的相对丰度和厚壁菌门/拟杆菌门比值显著降低(P<0.05),拟杆菌门的相对丰度显著提高(P<0.05);在属水平上,与MD组相比,PD组克里斯滕森氏菌科R-7群、UCG-002、未分类厚壁菌门的相对丰度显著降低(P<0.05),未分类鼠杆菌科、普雷沃氏菌属、未分类梭菌属_UCG-014的相对丰度显著提高(P<0.05)。综上所述,与乳源蛋白代乳粉相比,植物源蛋白代乳粉会降低羔羊生长性能和消化性能;此外,试验中植物源蛋白代乳粉会促进羔羊瘤胃发酵,改变瘤胃中微生物菌群结构。

关键词：羔羊代乳粉生长性能消化性能瘤胃发酵瘤胃微生物区系

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Rationalizing the isolation distance needed for field trials involving genetically modified rapeseed (Brassica napus L.) in China

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Chinese Science Bulletin 2013年第13期58卷 1558-1567页

作者： ZHAO XiangXiang TANG Tang CHEN GuiMin LIU FuXia WANG XingLong BU CuiPing lu changming Key Laboratory of Oil Crop Biology of Ministry of Agriculture Oilcrops Research InstituteChinese Academy of Agricultural Sciences Jiangsu Key Laboratory for Eco-Agricultural Biotechnology around Hongze Lake Huaiyin Normal University

The isolation distance required for field trials of genetically modified (GM) rapeseed varies widely worldwide, with a 50-400 m distance in most nations contrasting with a minimum 1000-m isolation distance in China. The goal of this study was to evaluate the relevance of current regulations in China regarding the isolation distance needed for GM rapeseed trials. A pollen flow experiment was conducted based on the design of concentric circles, with the GM plants in a 20-m diameter circle at the centre, surrounded by non-GM plants to a distance 60 m from the perimeter of the circle containing GM plants. The rate of pollen flow was the highest at the isolation distance of 0.5 m, where it ranged from 2.3091% to 2.6711%. The general pattern of the pollen flow rate (y) with distance (x) was well described by the equation y = 1.3936x-0.9136 (R 2 = 0.9950). The long-distance pollen flow tested at the isolation distance of 800 m was 0.0018%, which agrees with the theoretical prediction. The results suggested that 300 m, rather than 1000 m, is a reasonable distance to ensure a tolerable threshold of pollen flow (less than 0.01%) under conditions of winter rapeseed production in China.

关键词：转基因油菜籽隔离距离现场试验中国转基因植物花粉流世界范围田间试验

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Long Chain Acyl-Coenzyme A Synthetase 4(BnLACS4) Gene from Brassica napus Enhances the Yeast Lipid Contents

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Journal of Integrative Agriculture 2014年第1期13卷 54-62页

作者： TAN Xiao-li ZHENG Xiang-feng ZHANG Zhi-yan WANG Zheng XIA Heng-chuan lu changming GU Shou-lai Institute of Life Sciences Jiangsu University Oil Crops Research Institute Chinese Academy of Agricultural Sciences

Long-chain acyl-Coenzyme A （CoA） synthetases （LACSs） catalyze the formation of long-chain acyl-CoA, and play important roles in fatty acid metabolism including phospholipids, triacylglycerol （TAG） biosynthesis and fatty acid 13-oxidation. Here, we report the characterization of a LACS gene from Brassica napus. It is highly homologous to Arabidopsis LACS4 and thus designated as BnLACS4. The cloned gene BnLACS4 could complement a LACS-deficient yeast strain YB525. It is mainly expressed in flowers and developing seeds where lipid biosynthesis is at high rate in Brassiea napus. When transiently expressed in tobacco leaves, BnLACS4 is localized in endoplasmic reticulum （ER）, the common site for eukaryotic pathway of lipid biosynthesis. Expression of BnLACS4 in the yeast strain pep4 increased its lipid content. Taken together, our results suggest that BnLACS4 may be involved in lipid biosynthesis in B. napus.

关键词： Brasscia napus LACS lipid subcellular localization yeast

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RNA-seq profiling the transcriptome of secondary seed dormancy in canola(Brassica napus L.)

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Chinese Science Bulletin 2014年第32期59卷 4341-4351页

作者： Fuxia Liu Xiangqiang Zhao Lihua Zhang Tang Tang changming lu Guiming Chen Xinlong Wang Cuiping Bu Xiangxiang Zhao Jiangsu Key Laboratory for Eco-Agricultural Biotechnology around Hongze Lake Huaiyin Normal University School of Life Sciences Nantong University Plant science department South Dakota State UniversityBrookings SD 57006 USA Genetic Engineering and GMO Biosafety Lab of Oil Crops Research Institute Chinese Academy of Agricultural Sciences

Secondary seed dormancy(SSD)is responsible for volunteer plants in canola fields,which causes a series of problems in canola production and serves as an important trait for the environmental safety assessment of transgenic canola.A canola cultivar with strong SSD was used to establish insight into seed transcriptomes in its secondarily dormant seeds and control seeds without dormancy by RNA-seq analysis,aiming to determine the molecular ecological characterizations of SSD.A dataset(more than 4 Gb)of valid sequences was obtained from each sample,which was combined to carry out the de novo *** assembled sequences consisted of 314,261fragments with length[100 bp,including 29,740 long transcripts of length C 500 *** annotation indicated that 1,641 long transcripts could be categorized into 24 cluster of orthologous groups of proteins(COGs)and 16,515 transcripts were linked to 2,648 gene ontology(GO)*** were 452 long transcripts with significantly different expression identified by a threshold of[2-fold expression change(P\0.001)between samples,among which 343 transcripts were unambiguously homologous to Arabidopsis *** plant hormones abscisic acid and gibberellins were known as the pivotal regulators of seed dormancy and *** genes responsible for either biosynthesis or signaling of each hormone could be widely verified from the SSD transcriptome,theirs expression evidences failed to correlate with the induction of *** on the enriched terms of gene ontology and KEGG orthology,as well as the expression models of candidate genes of SSD,we proposed that fatty acid metabolism might implicate in SSD in *** information reported here may play a significant role in further understanding of environmental safety assessment of SSD in transgenic canola.

关键词：种子休眠转录物油菜田二级 Q分析转基因油菜双低油菜籽基因本体论

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Rotating tank experiments for the study of geophysical fluid dynamics

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Journal of Oceanology and Limnology 2024年第5期42卷 1385-1398页

作者： changming DONG Xiaojie lu Yuli LIU Guoqing HAN Minghan FU Qian CAO Yang ZHANG Xu CHEN Yeping YUAN International Geophysical Fluid Dynamics Laboratory Nanjing University of Information Science and TechnologyNanjing 210044China Department of Atmospheric and Oceanic Sciences University of CaliforniaLos Angeles CA 90095USA State Key Laboratory of Satellite Ocean Environment Dynamics Second Institute of OceanographyMinistry of Natural ResourcesHangzhou 310012China Southern Marine Science and Engineering Guangdong Laboratory(Zhuhai) Zhuhai 519000China Zhejiang Ocean University Zhoushan 316022China Guangdong Ocean University Zhanjiang 524088China Ocean University of China Qingdao 266100China Zhejiang University Hangzhou 310058China

Geophysical fluid dynamics(GFD)is an interdisciplinary field that studies the large-scale motion of fluids in the natural *** a wide range of applications such as weather forecasts and climate prediction,GFD employs various research approaches including in-situ observations,satellite measurements,numerical simulations,theoretical analysis,artificial intelligence,and physical model experiments in *** these approaches,rotating tank experiments provide a valuable tool for simulating naturally-occurring fluid motions in *** proportional scaling and proper techniques,scientists can reproduce multi-scale physical processes of stratified fluids in the rotation system,which allows for the simulation of essential characteristics of fluid motions in the atmosphere and *** this review,rotating tanks of various scales in the world are introduced,as these tanks have been actively used to explore fundamental scientific questions in ocean and atmosphere *** illustrate the GFD experiments,three representative cases are presented to demonstrate the frontier achievements in the the GFD study by using rotating tank experiments:mesoscale eddies in the ocean,convection processes,and plume *** references for the experimental procedures are *** studies are encouraged to further explore the utilization of rotating tanks with improvements in experimental design and integration of other research *** is a promising direction of GFD to help enhance our understanding of the complex nature of fluid motions in the natural world and to address the challenges posed by global environmental changes.

关键词： laboratory experiment rotating tank geophysical fluid dynamics(GFD)

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整株转化法及其在油菜上的应用与展望

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植物学报 2009年第2期44卷 236-244页

作者：邹智卢长明中国农业科学院油料作物研究所基因工程与转基因安全研究室武汉430062

近年来,在植物遗传转化和功能基因组学研究的迫切需求下,一些不依赖于受体细胞脱分化、再分化过程的转化技术(即所谓的整株转化法)相继出现,它们大多以分生组织或生殖细胞作为受体,不受基因型限制,具有操作简单、转化效率高、无体细胞... 详细信息

近年来,在植物遗传转化和功能基因组学研究的迫切需求下,一些不依赖于受体细胞脱分化、再分化过程的转化技术(即所谓的整株转化法)相继出现,它们大多以分生组织或生殖细胞作为受体,不受基因型限制,具有操作简单、转化效率高、无体细胞变异和后代遗传稳定等优点。该文对整株转化法的最新研究进展进行综述,并重点讨论了其在油菜(Brassica napus)上的应用现状与前景。

关键词：根癌农杆菌油菜整株转化法分生组织生殖细胞

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小释企业专利战略中专利思想的基本原则

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电子知识产权 2007年第5期 61-62,65页

作者：逯长明北京集佳知识产权代理有限公司

问：知识产权战略能够决定一个企业长期的知识产权行为规划，那么企业怎样才能设计出一个有效的专利战略，专利战略的调整依据是什么？作为宏观专利指导的专利战略与其具体的实施步骤或过程的关系又是怎样的，实践中应当如何把握实施细... 详细信息

问：知识产权战略能够决定一个企业长期的知识产权行为规划，那么企业怎样才能设计出一个有效的专利战略，专利战略的调整依据是什么？作为宏观专利指导的专利战略与其具体的实施步骤或过程的关系又是怎样的，实践中应当如何把握实施细节呢？

关键词：企业专利战略知识产权战略行为规划设计才能宏观应当实践

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商业方法类程序专利保护的处理方法

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电子知识产权 2005年第4期 59-62页

作者：逯长明集佳知识产权代理有限公司

问:在专利代理实践中,怎样判断商业方法类程序的专利性?又该如何对其进行处理呢?答:商业方法类程序由于涉及较多的商业交易规则,对于是否可被授予专利权以及具体商业方法的专利性通常存在较大的争议.

关键词：商业方法处理方法专利保护程序专利代理专利性

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浅析公路动物防疫监督检查站建设存在的问题及对策

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中国动物检疫 2007年第4期24卷 17-18页

作者：林荣君卢长明南京市六合区兽医卫生监督所 211500 南京市江宁区畜牧兽医站 211100

随着社会主义市场经济不断发展．动物及动物产品地区间贸易将越来越频繁．一方面促进了畜牧业发展．另一方面也给动物疫病传播创造有利条件，动物疫情传播、扩散的风险不断地加大。为积极地应对动物及动物产品地区间频繁流动导致的疫情... 详细信息

随着社会主义市场经济不断发展．动物及动物产品地区间贸易将越来越频繁．一方面促进了畜牧业发展．另一方面也给动物疫病传播创造有利条件，动物疫情传播、扩散的风险不断地加大。为积极地应对动物及动物产品地区间频繁流动导致的疫情传播风险．一些省市依据《动物防疫法》规定．特别是在发生突发重大动物疫情时．在公路交通枢纽设置公路动物防疫监督检查站．对过境运输动物及动物产品实施了防疫监督检查。公路动物防疫监督检查站的设立．对降低异地动物疫病传入的风险起到了重要保障作用。但是．公路动物防疫检查站自身的、社会的因素影响．常常是建了撤。撤了建．始终处于动荡状态。笔者从事公路动物防疫监督检查工作多年．现就公路动物防疫监督检查站建设谈一点个人体会。

关键词：公路动物防疫监督检查站社会主义市场经济《动物防疫法》动物产品重大动物疫情监督检查工作疫情传播畜牧业发展

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