检索结果-南通市图书馆

Development and Application of a Chemical Labeling-Based Biosensing Assay for Rapid Detection of 8-Oxoguanine and Its Repair in vitro and in Human Cells

在线全文

学校读者我要写书评

暂无评论

Chinese Journal of Chemistry 2022年第19期40卷 2329-2334页

作者： Weiheng Kong Yongqin Ji Xiaowen Zhu Xiaoxia Dai changjun you Molecular Science and Biomedicine Laboratory State Key Laboratory of Chemo/Biosensing and ChemometricsCollege of Chemistry and Chemical EngineeringHunan Provincial Key Laboratory of Biomacromolecular Chemical BiologyHunan UniversityChangshaHunan410082 China College of Chemistry and Chemical Engineering Qufu Normal UniversityQufuShandong273165 China

Living cells are constantly threatened by endogenous and environmental agents that can induce various DNA lesions including 8-oxoguanine(8-oxoG).Increasing evidence has suggested that 8-oxoG is not only a biomarker of oxidative stress,but also a novel epigenetic-like modification involved in transcriptional regulation in mammalian *** of DNA damage and repair is useful for both basic research and clinical applications,but current methods for 8-oxoG detection still suffer from some problems such as poor selectivity,time consuming and being ***,we developed a fast and simple biosensing approach for quantitative analysis of 8-oxoG in DNA,which was based on the selective chemical biotinylation of 8-oxoG in conjunction with biotin-streptavidin enzyme-linked immunosorbent *** have also successfully applied this method to achieve efficient detection of the repair activities of DNA glycosylases Fpg and hOGG1 toward 8-oxoG in vitro and in human *** newly developed biosensing assay should be generally applicable for rapid detection of 8-oxoG and its repair in other organisms.

关键词： 8-Oxoguanine Oxidative damage Biosensors Analytical methods Biological chemistry and chemical biology

Next-generation sequencing-based analysis of the effect of N^(6)-methyldeoxyadenosine modification on DNA replication in human cells

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Chinese Chemical Letters 2022年第4期33卷 2077-2080页

作者： Juan Wang Yuwei Sheng Ying Yang Xiaoxia Dai changjun you Molecular Science and Biomedicine Laboratory State Key Laboratory of Chemo/Biosensing and ChemometricsCollege of Chemistry and Chemical EngineeringHunan Provincial Key Laboratory of Biomacromolecular Chemical BiologyHunan UniversityChangsha 410082China

N^(6)-methyldeoxyadenosine(6 mdA) modification is considered as a new epigenetic mark that may play important roles in various biological ***,it remains unclear about the effect of 6 mdA on DNA replication in human ***,we combined next-generation sequencing with shuttle vector technology to explore how 6 mdA affects the efficiency and accuracy of DNA replication in human *** results showed that 6 mdA neither blocked DNA replication nor induced mutations in human ***,we found that the depletion of translesion synthesis DNA polymerase(Pol) κ,Pol η,Pol ι or Pol ζ did not significantly change the biological consequences of 6 mdA during replication in human *** negligible impact of 6 mdA on DNA replication is consistent with its potential role in epigenetic gene expression.

关键词： N^(6)-methyldeoxyadenosine DNA replication Next-generation sequencing Shuttle vector technology Translesion synthesis DNA polymerase

Comprehensive profiling of CTP-binding proteins using a biotinylated CTP affinity probe

在线全文

学校读者我要写书评

暂无评论

Chinese Chemical Letters 2021年第11期32卷 3479-3482页

作者： Mengting Pa Yunming Liu Xiaofang Zheng Meijuan Zhou changjun you Xiaoxia Dai State Key Laboratory of Chemo/Biosensing and Chemometrics College of Chemistry and Chemical EngineeringMolecular Science and Biomedicine LaboratoryHunan Provincial Key Laboratory of Biomacromolecular Chemical BiologyHunan UniversityChangsha 410082China

Recent studies have shown that CTP may act as a ligand to regulate the activity of its target proteins in many biological ***,proteome-wide identification of CTP-binding proteins remains ***,we employed a biotinylated CTP affinity probe coupled with stable isotope labeling by amino acids in cell culture(SILAC)-based quantitative proteomics approach to capture,identify and quantify CTP-binding proteins in human *** performing two types of competitive SILAC experiments with high *** concentrations of CTP probe(100 vs.10µmol/L)or with CTP probe in the presence of free CTP,we identified 90 potential CTP-binding proteins which are involved in a variety of biological processes,including protein folding,nucleotide binding and cell-cell ***,we developed a chemical proteomic method for uncovering the CTP-binding proteins in human cells,which could be widely applicable for profiling CTP-binding proteins in other biological samples.

关键词： CTP-binding proteins CTP affinity probe Mass spectrometry SILAC Quantitative proteomics

STRAP binds to and promotes the repair of N1-methyldeoxyadenosine in DNA

在线全文

学校读者我要写书评

暂无评论

Chinese Chemical Letters 2024年第3期35卷 204-207页

作者： Meijuan Zhou Qian Liu Mengting Pan Ying Yang Xiaoxia Dai changjun you State Key Laboratory of Chemo/Biosensing and Chemometrics Hunan Provincial Key Laboratory of Biomacromolecular Chemical BiologyMolecular Science and Biomedicine LaboratoryCollege of Chemistry and Chemical EngineeringHunan UniversityChangsha 410082China

N1-methyladenosine(m1A)is an important RNA modification that functions in various biological processes by interacting with cellular ***,the binding proteins of N1-methyldeoxyadenosine(1mdA)in DNA remain largely ***,we employed a quantitative proteomics strategy to identify the potential binding proteins of 1mdA in human *** results revealed that serine–threonine kinase receptor-associated protein(STRAP)can bind to 1mdA-carrying *** further demonstrated that STRAP participates in alkylating agent-induced DNA damage response and can promote the repair of 1mdA embedded in ***,we investigated the effects of STRAP on 1mdA-induced perturbation in transcription using a shuttle vector-and next-generation sequencing-based assay,and found that STRAP is involved in the transcriptional bypass of 1mdA in human ***,our study revealed STRAP as a novel 1mdA-binding protein in human cells and provided new insight into the biological implications of STRAP and 1mdA modification in human diseases.

关键词： N1-methyldeoxyadenosine 1mdA-binding protein STRAP DNA repair Transcription

Chemically bonded Mn_(0.5)Cd_(0.5)S/BiOBr S-scheme photocatalyst with rich oxygen vacancies for improved photocatalytic decontamination performance

在线全文

学校读者我要写书评

暂无评论

Advanced Powder Materials

Advanced Powder Materials 2024年第3期3卷 12-22页

作者： Shijie Li changjun you Ke Rong Chunqiang Zhuang Xiaobo Chen Bin Zhang Key Laboratory of Health Risk Factors for Seafood of Zhejiang Province National Engineering Research Center for Marine AquacultureCollege of Food Science and PharmacyZhejiang Ocean UniversityZhoushan 316022China Institute of Microstructure and Property of Advanced Materials Faculty of Materials and ManufacturingBeijing University of TechnologyBeijing 100124China Department of Chemistry University of MissouriKansas CityMO 64110USA

Devising exceptional S-scheme heterojunction photocatalysts utilized in annihilating pharmaceuticals and chromium contamination is significant for addressing the problem of global water *** this work,a chemically bonded Mn0.5Cd_(0.5)S/BiOBr S-scheme heterostructure with oxygen vacancies is ingeniously developed through a facile in-situ solvothermal *** designed Mn0.5Cd_(0.5)S/BiOBr heterojunction exhibits eminently reinforced photo-activity for destruction of tetracycline hydrochloride and Cr(VI)as compared with its individual *** substantial photo-redox performance amelioration is benefitted from the creation of an intense internal electric field(IEF)via supplying powerful driving force and migration highway by interfacial chemical bond to foster the S-scheme electron/hole *** intriguingly,the IEF at the hetero-interface drives the fast consumption of the photo-induced holes in Mn0.5Cd_(0.5)S by the photoelectrons from BiOBr,profoundly boosting the enrichment of active photo-carriers and sparing the photo-corrosion of Mn0.5Cd_(0.5)***,Mn0.5Cd_(0.5)S/BiOBr with exceptional anti-interference property can work efficiently in real water *** uses of the recycled Mn0⋅5Cd0⋅5S/BiOBr evidence its prominent robustness and *** achievement indicates the vast potential of chemically bonded S-scheme photosystems with structural defects in the design of photo-responsive materials for effective wastewater treatment.

关键词： Mn_(0.5)Cd_(0⋅5)S/BiOBr Chemically bonded S-Scheme Internal electric field Oxygen vacancies Antibiotic removal Photocatalysis

Position-dependenteffects ofregioisomericmethylated adenine and guanine ribonucleosideson translation

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Position-dependenteffects ofregioisomericmethylated adenine ...

第十届全国化学生物学学术会议

作者： changjun you Xiaoxia Dai Yinsheng Wang Department of Chemistry University of California State Key Laboratory of Chemo/Bio-Sensing and Chemometrics College of Chemistry and Chemical Engineering Hunan University

Reversible methylation of the N6 or N1 position of adenine in RNA has recently been shown to play significantrolesin regulating the functions of *** can also be alkylated uponexposure to endogenous and exogenous *** we developed a novelmass spectrometry-based assay toexaminehow regio-specific methylation at the hydrogen bonding edge of adenine and guanine in mRNA affectstranslation. When situated at the third codon position, themethylated nucleosides did not compromise the speedoraccuracy of translationunder most *** located at thefirst or second codon position, N1-methyladenosine（m1 A） and m1 G constituted robustblocks to both Escherichia coliand wheat germ extract translation systems, whereas N2-methylguanosine（m2 G） moderately impedes translation. Whilem1 A,m2 G, and N6-methyladenosine（m6 A）did not perturb translational fidelity,O6-methylguanosine（m6 G）at the first and second codon positionswas strongly andmoderately miscoding, respectively, and it was decoded as an adenosine withboth *** effects of methylated ribonucleosideson translation could be attributed to the methylation-elicited alterations in base pairing propertiesofthe nucleobases. Together, our study afforded important new knowledge about the modulation of translation by methylationof purine nucleobases in mRNA.

关键词： RNA methylation Translation Mass spectrometry

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

利用NTP和标准并口实现高精度时统同步

电子技术（上海） 2018年第6期47卷 76-77,61页

作者：尤长军江苏自动化研究所江苏连云港222061

针对高精度时统同步系统硬件成本高的问题,文章提出并设计实现了利用NTP协议和标准并口读取时间信息报文和精确对时脉冲的时间同步方法。实践结果表明,该方法既能保证微秒级的对时精度,又可有效节约硬件成本。

关键词：时统网络并口 ACK

在线全文

学校读者我要写书评

暂无评论

高职院校学生干部团队文化建设的探索

辽宁农业职业技术学院学报 2019年第2期21卷 49-51页

作者：尤长军辽宁农业职业技术学院辽宁营口115009

依据团队文化建设理论,分析了现阶段高职院校学生干部团队文化建设中存在的突出问题,提出了解决学生干部队伍团队文化建设困难的有效方法,以期形成具备完整系统优秀团队文化的高职学生干部队伍,促进高职院校大学生思想政治教育工作。

关键词：高职院校学生干部团队文化建设

Evolutionary Particle Swarm Optimization Algorithm Based on Collective Prediction for Deployment of Base Stations

在线全文

学校读者我要写书评

暂无评论

Computers, Materials & Continua 2025年第1期82卷 345-369页

作者： Jiaying Shen Donglin Zhu Yujia Liu Leyi Wang Jialing Hu Zhaolong Ouyang changjun Zhou Taiyong Li School of Computer Science and Technology Zhejiang Normal UniversityJinhua321004China School of Future Technologies Jiangxi Institute of Applied Science and TechnologyNanchang330000China School of Computing and Artificial Intelligence Southwestern University of Finance and EconomicsChengdu611130China

The wireless signals emitted by base stations serve as a vital link connecting people in today’s society and have been occupying an increasingly important role in real *** development of the Internet of Things(IoT)relies on the support of base stations,which provide a solid foundation for achieving a more intelligent way of *** a specific area,achieving higher signal coverage with fewer base stations has become an urgent ***,this article focuses on the effective coverage area of base station signals and proposes a novel Evolutionary Particle Swarm Optimization(EPSO)algorithm based on collective prediction,referred to herein as *** a new strategy called neighbor-based evolution prediction(NEP)addresses the issue of premature convergence often encountered by *** also employs a strengthening evolution(SE)strategy to enhance the algorithm’s global search capability and efficiency,ensuring enhanced robustness and a faster convergence speed when solving complex optimization *** better adapt to the actual communication needs of base stations,this article conducts simulation experiments by changing the number of base *** experimental results demonstrate thatunder the conditionof 50 ormore base stations,ECPPSOconsistently achieves the best coverage rate exceeding 95%,peaking at 99.4400%when the number of base stations reaches *** results validate the optimization capability of the ECPPSO algorithm,proving its feasibility and *** ablative experiments and comparisons with other algorithms highlight the advantages of ECPPSO.

关键词： Particle swarm optimization effective coverage area global optimization base station deployment