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Physiological and molecular studies of staygreen caused by pod removal and seed injury in soybean

The Crop Journal 2016年第6期4卷 435-443页

作者： Xinxin Zhang Min Wang Tingting Wu Cunxiang Wu Bingjun jiang changhong Guo Tianfu Han Key Laboratory of Molecular Cytogenetics and Genetic Breeding of Heilongjiang College of Life Science and TechnologyHarbin Normal University MOA Key Laboratory of Soybean Biology (Beijing) Institute of Crop Sciencethe Chinese Academy of Agricultural Sciences

Leaves provide substances and signals for pod and seed development in ***,the regulatory feedbacks of pod and seed to leaf development remain *** investigated the effects of pod and seed on leaf senescence by conducting pod removal and seed injury *** removal and seed injury delayed leaf senescence and caused the staygreen phenotype of *** were dosage effects of pod number on the extent of staygreen in depodded *** concentrations of chlorophyll(SPAD value,an index of relative chlorophyll content),soluble protein,and soluble sugar in the leaves of depodded plants were higher than those of intact *** seed development,the content of IAA decreased,while that of ABA *** trend was more pronounced in intact than in depodded and seed-injured *** GA3/ABA ratio decreased gradually in all *** content of GA3 was relatively stable and was higher in intact than in depodded *** expression levels of four senescence-related genes,Gm SARK,Gm SGR1,Gm CYN1,and Gm NAC,declined in depodded or seed-injured treatments and were positively correlated with the number of leaves retained on *** FT2 a,the major flowering-promoting gene,was expressed at a higher level while E1,a key flowering inhibitory gene,was expressed at a lower level in depodded than in intact *** propose that the pod or seed can regulate leaf *** the seed is aborted owing to disease infection or pest attack,the leaves stay green because of the absence of the seed signals for senescence.

关键词： Soybean Seed injury Staygreen Source–sink relationship

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Reliability evaluation of IGBT power module on electric vehicle using big data

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Journal of Semiconductors 2024年第5期45卷 50-60页

作者： Li Liu Lei Tang Huaping jiang Fanyi Wei Zonghua Li changhong Du Qianlei Peng Guocheng Lu The School of Electrical Engineering Chongqing UniversityChongqing 400044China The Deepal Automobile Technology Co. Ltd.Chongqing 400023China

There are challenges to the reliability evaluation for insulated gate bipolar transistors(IGBT)on electric vehicles,such as junction temperature measurement,computational and storage *** this paper,a junction temperature estimation approach based on neural network without additional cost is proposed and the lifetime calculation for IGBT using electric vehicle big data is *** direct current(DC)voltage,operation current,switching frequency,negative thermal coefficient thermistor(NTC)temperature and IGBT lifetime are *** the junction temperature(T_(j))is *** the rain flow counting method,the classified irregular temperatures are brought into the life model for the failure *** fatigue accumulation method is then used to calculate the IGBT *** solve the limited computational and storage resources of electric vehicle controllers,the operation of IGBT lifetime calculation is running on a big data *** lifetime is then transmitted wirelessly to electric vehicles as input for neural *** the junction temperature of IGBT under long-term operating conditions can be accurately estimated.A test platform of the motor controller combined with the vehicle big data server is built for the IGBT accelerated aging ***,the IGBT lifetime predictions are derived from the junction temperature estimation by the neural network method and the thermal network *** experiment shows that the lifetime prediction based on a neural network with big data demonstrates a higher accuracy than that of the thermal network,which improves the reliability evaluation of system.

关键词： IGBT junction temperature neural network electric vehicles big data

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不同密度拉巴豆与旱地玉米间作栽培对混合青贮饲料品质的影响

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中国饲料 2022年第14期 13-18页

作者：李文彬柴明杰张斌孔新利朱俊峰蒋长虹申世瑛郑炳辉梁天柱李雪瑛平凉市畜牧渔业站甘肃平凉744000 泾川县党原镇畜牧兽医站甘肃平凉744000 庄浪县农业技术推广中心甘肃平凉744000

文章旨在通过设计不同密度拉巴豆与旱地玉米间作栽培,研究其对混合青贮饲料品质的影响。试验设为11个处理,每个处理3个重复。对照组(D0)为玉米单播,试验组(X1~X10)为不同密度拉巴豆(3000~12000株/亩)与旱地玉米间作栽培。研究发现,(1)... 详细信息

文章旨在通过设计不同密度拉巴豆与旱地玉米间作栽培,研究其对混合青贮饲料品质的影响。试验设为11个处理,每个处理3个重复。对照组(D0)为玉米单播,试验组(X1~X10)为不同密度拉巴豆(3000~12000株/亩)与旱地玉米间作栽培。研究发现,(1)生物产量:X8组显著高于其他组(P<0.05),X5组显著高于D0组(P<0.05),随着密度的增加,生物产量先增加至10000株/亩后开始下降;(2)干物质含量:X1、X2、X5、X6、X9组显著高于其他组(P<0.05),以X5组为中心随着密度增加,呈现先增加后减少的趋势;(3)粗蛋白质含量:X8、X10组显著高于X4、X7、X9组(P<0.05),试验组除X7组外其他组均显著高于对照组(P<0.05),随密度增加有微弱增加的趋势;(4)水溶性碳水化合物含量:X2组显著高于其他组(P<0.05),X4、X5组显著高于D0、X1、X3、X7、X8、X9组(P<0.05),随密度增加呈逐渐降低的趋势;(5)总可消化养分含量:X4组显著高于X1、X2、X5、X6、X7组(P<0.05),随密度增加呈先增加后减少的趋势。(6)pH:X7组显著低于X5组(P<0.05),其他组之间差异不显著,随密度增加呈先增大后减小的趋势;(7)7 h消化率:X9组显著高于X1、X2、X5、X6、X7、X8组(P<0.05),随密度增加呈先降低后升高的趋势。结论:拉巴豆与旱地玉米间作栽培能显著提高混合青贮饲料干物质、粗蛋白质含量,提高混合青贮饲料营养品质。密度为10000株/亩时,虽然生物产量最高,但混合青贮饲料干物质含量较低。因此,综合各项指标建议拉巴豆间作密度为6000~7000株/亩。

关键词：拉巴豆玉米间作密度混合青贮营养品质

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不同覆膜形式对旱地玉米—拉巴豆间作栽培饲料产量及青贮品质的影响

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中国饲料 2023年第4期 78-83页

作者：高应平郑炳辉梁天柱柴明杰申世瑛朱俊峰李文彬郭柏宏蒋长虹李巧红庄浪县农业技术推广中心甘肃平凉744600 平凉市畜牧渔业站甘肃平凉744000

文章旨在研究不同覆膜形式的旱地玉米—拉巴豆间作栽培对青贮饲料品质的影响。试验采用单因素方差设计,玉米和拉巴豆间作栽培使用随机区组设计,设4个处理,分别为T0组(露地,对照组)、T1组(降解膜)、T2组(半膜)、T3组(全膜),每个处理3个重... 详细信息

文章旨在研究不同覆膜形式的旱地玉米—拉巴豆间作栽培对青贮饲料品质的影响。试验采用单因素方差设计,玉米和拉巴豆间作栽培使用随机区组设计,设4个处理,分别为T0组(露地,对照组)、T1组(降解膜)、T2组(半膜)、T3组(全膜),每个处理3个重复,共12个小区。结果表明,(1)各试验组折算亩产量显著高于对照组,且T3组产量显著高于对照组和T1、T2组。(2)T1组粗蛋白质含量显著低于对照组和T3组(P 0.05)。T1组酸性洗涤纤维含量显著低于T2组(P 0.05)。(3)T3组氨含量显著高于对照组和T1组(P 0.05),T2组与对照组乳酸浓度差异不显著(P> 0.05)。综合考虑,结合西北旱地农业实际,T3组即全膜的覆膜形式可推荐为最佳覆膜形式。

关键词：覆膜形式青贮玉米拉巴豆间作栽培青贮品质

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Cinobufacini-induced HeLa cell apoptosis enhanced by curcumin

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Chinese Science Bulletin 2013年第21期58卷 2584-2593页

作者： LIU Li JIN Hua OU JinLai jiang JinHuan PI jiang KE changhong YANG Fen QIAO DongJuan CAI HuaiHong CAI JiYe Department of Chemistry and Institute for Nano-Chemistry Jinan University Department of Pharmaceutics College of Pharmacy Jinan University

When used in combination with certain chemotherapies, curcumin has been shown to increase apoptosis in several cancer cell lines. Here, we report the combined effects of curcumin and cinobufacini on human cervical carcinoma cells. The aim of this study was to examine whether curcumin could enhance apoptosis induced by cinobufacini. 3-(4,5-Dimethylthiazol-2-y1)-2,5- diphenytetrazolium bromide (MTT) assays revealed that the growth and proliferation of HeLa cells could be inhibited by 75% after a combined treatment of 25 μg/mL cinobufacini and 8 μg/mL curcumin. The combined treatment is 3 times more effective than treatment with 25 μg/mL cinobufacini alone. Annexin V-FITC/PI staining, morphological changes and immunofluorescence verified a significant enhancement in cinobufacini-induced apoptosis when cells were also exposed to curcumin. The data showed that the proportion of early apoptotic cells significantly increased from 15.43% in cells treated only with 25 μg/mL cinobufacini to 49.2% in cells treated with 25 μg/mL cinobufacini and 8 μg/mL curcumin. Moreover, compared with treatment of only 25 μg/mL cinobufacini, ROS production increased 1.7-fold, the intracellular free Ca 2+ concentration increased 1.5-fold, and the mitochon- drial membrane potential decreased by 20% in the combined treatment. Simultaneously, the atomic force microscopy (AFM) re- sults suggest that cells treated with a combination of cinobufacini and curcumin varied significantly in shape and ultrastructure. Collapsed cells with leaking cytoplasm, blebbing pores and emerging apoptotic bodies were prevalent. The nanoparticle size increased from 70 nm when the cells were treated with 25 μg/mL cinobufacini to 190 nm when the cells were treated with 25 μg/mL cinobufacini and 8 μg/mL curcumin. The size increase resulted in the cell membrane becoming considerably rough. These results can improve our understanding of combination treatments. Specifically, the combination of cinobufacini and curcumin may

关键词： HeLa细胞细胞凋亡姜黄素诱导原子力显微镜超微结构变化蟾联合治疗

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一种新型充气式重力梯度杆的研制和在轨展开试验

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航天返回与遥感 2014年第3期35卷 20-27页

作者：曹旭王伟志张宏伟王永滨江长虹谭惠丰卫剑征北京空间机电研究所北京100094 哈尔滨工业大学复合材料与结构研究所哈尔滨150080

文章对中国"新技术验证一号"卫星搭载的一种新型充气式重力梯度杆的研制和在轨试验情况进行了介绍。重力梯度杆在小卫星控制领域中具有较好的应用前景,而充气结构具有轻质、占用空间小、发射成本低的特点,采用充气式重力梯度杆可避免传... 详细信息

文章对中国"新技术验证一号"卫星搭载的一种新型充气式重力梯度杆的研制和在轨试验情况进行了介绍。重力梯度杆在小卫星控制领域中具有较好的应用前景,而充气结构具有轻质、占用空间小、发射成本低的特点,采用充气式重力梯度杆可避免传统机械式重力梯度杆质量重、尺寸大和机构复杂的缺点,它以充气伸展臂为主要结构形式,通过充气展开伸直成为重力梯度杆,既作为卫星重力梯度杆又通过在轨试验验证空间充气展开结构的性能。在轨试验结果表明充气式重力梯度杆展开稳定、有序、性能可靠,可以满足小卫星的性能和功能要求。该试验也是中国首个成功的充气展开结构在轨技术验证试验,标志着中国的充气展开结构技术初步具备了工程化能力。

关键词：充气展开结构技术充气式重力梯度杆研制在轨展开

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丹霞地貌桫椤群落土壤养分及有机质的垂直分异

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山地农业生物学报 2021年第4期40卷 12-17页

作者：蒋长洪漆基海翁涛穆君白小节高露胡小润臧丽鹏何跃军贵阳市城市绿化服务中心贵州贵阳550003 贵州大学林学院/贵州大学森林生态研究中心贵州贵阳550025 贵州省赤水桫椤国家级自然保护区管理局贵州赤水564700

黔北丹霞地貌是桫椤(Alsophila spinulosa)种群集中分布区,不同物种组成的桫椤群落在土壤养分是如何随土层深度垂直分布的特征缺乏关注。探索桫椤群落土壤养分及有机质含量随土层深度的变化特征,对深入探索桫椤种群濒危机制及保育技术... 详细信息

黔北丹霞地貌是桫椤(Alsophila spinulosa)种群集中分布区,不同物种组成的桫椤群落在土壤养分是如何随土层深度垂直分布的特征缺乏关注。探索桫椤群落土壤养分及有机质含量随土层深度的变化特征,对深入探索桫椤种群濒危机制及保育技术具有重要意义。论文选择贵州赤水和习水国家自然保护区丹霞地貌4个典型桫椤群落样地进行样方调查,并采集0~10 cm、10~20 cm、20~30 cm土壤样品,测定土壤全氮、全磷、全钾、碱解氮、速效磷、速效钾及有机质含量,分析不同群落样地和不同土层深度对土壤性质的影响。结果表明:不同桫椤群落类型和土层深度共同影响土壤全氮和有效态氮、磷、钾含量和有机质含量,且二者交互作用对有效态养分和有机质显著影响;土壤全氮、碱解氮、速效钾和有机质含量,N/P、N/K、P/K比率随土层深度增加而降低,而速效磷则是随土层深度增加而增加趋势。研究表明,黔北丹霞地貌桫椤群落土壤养分和有机质含量随群落物种组成和土层深度变化而垂直分异。

关键词：桫椤土壤养分有机质垂直分异

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宽阔水国家级自然保护区种养殖遗传资源调查

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贵州农业科学 2017年第4期45卷 85-88页

作者：杨应谢佩耘何跃军高明浪何敏红林艳蒋长洪司建鹏贵州大学林学院贵州贵阳550025 贵州省宽阔水国家级自然保护区管理局贵州绥阳563300

为宽阔水国家级自然保护区生物多样性的保护及开发利用提供参考,采用实地踏查和查阅文献资料方法对该保护区的种养殖种质资源进行调查。结果表明:保护区共有种养殖种质资源174个品种。植物种质资源有117种,其中,粮食作物类41种,如绥阳... 详细信息

为宽阔水国家级自然保护区生物多样性的保护及开发利用提供参考,采用实地踏查和查阅文献资料方法对该保护区的种养殖种质资源进行调查。结果表明:保护区共有种养殖种质资源174个品种。植物种质资源有117种,其中,粮食作物类41种,如绥阳谷、麻谷和三叶子玉米等;杂粮作物类10种,如乌洋芋和黄洋芋等;蔬菜作物类35种,如黄籽四季豆和朝天椒等;经济作物类12种,如绥阳油菜和绥阳马尾油菜等;经济林果类12种,重要野生植物种质资源有野生猕猴桃、野生天麻、山梨、野葡萄、桑葚、兰花和刺梨等7种。动物种质资源有43种,其中,家畜类17种,如绥阳猪等;家禽类12种,如绥阳鸡等。经济昆虫类2种,分别为意蜂和中蜂。水产鱼类有12种,如鲈鲤等。宽阔水国家级自然保护区具有丰富的种养殖遗传资源,外来品种引种栽培、养殖时应注重保护区本土遗传资源的保护和管理。

关键词：宽阔水国家级自然保护区种养殖遗传资源遗传多样性

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Single-cell transcriptome atlas reveals spatiotemporal developmental trajectories in the basal roots of moso bamboo (Phyllostachys edulis)

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Horticulture Research 2023年第8期10卷 59-71页

作者： Zhanchao Cheng changhong Mu Xiangyu Li Wenlong Cheng Miaomiao Cai Chongyang Wu Jutang jiang Hui Fang Yucong Bai Huifang Zheng Ruiman Geng Junlei Xu Yali Xie Yuping Dou Juan Li Shaohua Mu Jian Gao Key Laboratory of National Forestry and Grassland Administration/Beijing for Bamboo&Rattan Science and Technology International Center for Bamboo and RattanBeijing 100102China

Roots are essential for plant growth and *** is a large Poaceae perennial with 1642 species ***,little is known about the transcriptional atlas that underpins root cell-type ***,we set up a modified protocol for protoplast preparation and report single-cell transcriptomes of 14279 filtered single cells derived from the basal root tips of moso *** identified four cell types and defined new cell-type-specific marker genes for the basal *** reconstructed the developmental trajectories of the root cap,epidermis,and ground tissues and elucidated critical factors regulating cell fate *** to in situ hybridization and pseudotime trajectory analysis,the root cap and epidermis originated from a common initial cell lineage,revealing the particularity of bamboo basal root *** further identified key regulatory factors for the differentiation of these cells and indicated divergent root developmental pathways between moso bamboo and ***,PheWOX13a and PheWOX13b ectopically expressed in Arabidopsis inhibited primary root and lateral root growth and regulated the growth and development of the root cap,which was different from WOX13 orthologs in *** together,our results offer an important resource for investigating the mechanism of root cell differentiation and root system architecture in perennial woody species of Bambusoideae.

关键词： inhibited roots bamboo

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Progress on design and related R&D activities for the water-cooled breeder blanket for CFETR

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Theoretical & Applied Mechanics Letters 2019年第3期9卷 161-172页

作者： Songlin Liu Xiaoman Cheng Xuebin Ma Lei Chen Kecheng jiang Xia Li Hui Bao Jichao Wang Wanjing Wang changhong Peng Peng Lu Min Li Kai Huang Institute of Plasma Physics Chinese Academy of Sciences Hefei 230031 China Advanced Energy Research Center Shenzhen University Shenzhen 518060 China University of Science and Technology of China Hefei 230026

The water-cooled ceramic breeder (WCCB) blanket is one of the blanket candidates for Chinese fusion engineering testing reactor (CFETR) and is being developed at the Institute of Plasma Physics, Chinese Academy of Sciences (ASIPP). This paper reviews design and evolution of the WCCB blanket for CFETR, and presents a new WCCB blanket design according to the latest CFETR core parameters (major and minor radii are R = 7.2 m and a = 2.2 m, respectively) and missions. This new design is expected to satisfy multiple CFETR operation modes of 0.2, 0.5, 1.0, and 1.5 GW fusion power and achieve tritium self-sufficiency. The feasibility of the updated blanket design is evaluated from the aspects of neutronics and thermo-hydraulics. Furthermore, the research and development (R&D) activities supporting to the WCCB blanket for CFETR are reported, including the design code, the water loop experiments, the pebble bed modeling and experiments, and the components fabrication technology.

关键词： Chinese fusion engineering testing reactor WCCB blanket Research and development

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