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Bidirectional regulation of Ca^(2+) in exo–endocytosis coupling

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Science China(Life Sciences) 2018年第12期61卷 1583-1585页

作者： Qian Song Mingzhu Huang Bianbian wang Xinjiang Kang changhe wang Center for Mitochondrial Biology and Medicine The Key Laboratory of Biomedical Information Engineering of Ministry of Education School of Life Science and Technology and Frontier Institute of Science and Technology Xi'an Jiaotong University College of Life Sciences Liaocheng University DOE-Key Lab of Medical Electrophysiology Institute of Cardiovascular Research Southwest Medical University

Vesicular exocytosis is a key process involved in neurotransmitter release,whereas vesicle recycling is critical for the homeostasis of plasma membrane structures and the maintenance of ***^2+ regulates several fundamental cellular processes(Zhu et al.,2016,Xiong and Zhu,2016).Especially,Ca^2+ influx plays a dual role of triggering vesicular exocytosis and modulating compensatory endocytosis in neurons and endocrine cells.

关键词： Vesicular exocytosis key process

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Induced Dopaminergic Neurons for Parkinson's Disease Therapy: Targeting the Striatum or Midbrain/Substantia Nigra Pars Compacta?

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Neuroscience Bulletin 2022年第7期38卷 820-824页

作者： Huadong Xu Xu Cheng Qian Song Yuxin Yang changhe wang Xinjiang Kang Key Laboratory of Medical Electrophysiology Ministry of Education of ChinaThe Institute of Cardiovascular ResearchSouthwest Medical UniversityLuzhou 646000China Neuroscience Research Center Institute of Mitochondrial Biology and MedicineKey Laboratory of Biomedical Information Engineering of Ministry of EducationSchool of Life Science and TechnologyXi'an Jiaotong UniversityXi'an 710049China Department of Neurology The First Affliated Hospital of Xi'an Jiaotong UniversityXian 710061China College of Life Science Liaocheng UniversityLiaocheng 252059China

Parkinson's disease(PD)is a common neurodegenerative disease among the elderly,characterized by the specific loss of dopaminergic(DAergic)neurons in the substantia nigra pars compacta(SNpc)and defects in dopamine(DA)r... 详细信息

关键词： Dopamine Therapy dopamine

New Early Triassic paleo-magnetic data from Huangben section,Guangdong and its tectonic implications

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Chinese Science Bulletin 2006年第15期51卷 1894-1902页

作者： SHEN Zhongyue DING Jinghai YE changhe WEN Junwei wang Zhaoliang CHEN Zhifei Department of Earth Sciences Zhejiang University Hangzhou 310027 China

A paleomagnetic study was carried out on a total of 122 oriented cores from Daye Formation of the Huangben section, Guangdong Province. Rock magnetic investigations show that low-titanium magnetite is a dominant carrier. Detailed progressive thermal demagnetization isolated 2―3 components. The low temperature component clusters around the present geomagnetic field direction. An intermediate temperature component is isolated at 100―400℃ and is interpreted as a Yanshanian remagnetization. A high-temperature characteristic remanent mag- netization (ChRM) is obtained at 400―530℃ with D = 51.1°,I = 15.3°,α95 = 5.0° and passes generalized fold test and reversal test, suggesting high tempera- ture component is a primary remanence. Its corre- spondent pole position is 38.5°N, 212.7°E (dp = 2.6, dm = 5.1), which overlaps contemporaneous pole of Yangtze Block at 95% confidence level. Therefore, we believe that Huanan and Yangtze blocks were not separated in the Early Triassic. Compared to other published Early Triassic paleomagnetic data from Huanan Block, the inclinations are very close but the declinations are greatly variable. In stereo-projection, all the Early Triassic poles from Huanan Block lie along a small circle around the Huanan Block, im- plying that local micro-blocks rotations lead to the inconsistence of Early Triassic paleomagnetic direc- tions.

关键词：早三叠纪古磁学华南板快扬子江板快

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超声剪切波弹性成像联合AAR、APRI和FIB-4指数诊断慢性乙型肝炎患者肝纤维化程度效能研究

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实用肝脏病杂志 2025年第1期28卷 32-35页

作者：王常鹤朱璇邓静张聪颖赵宇刘芳佳木斯市妇幼保健院物理诊断科黑龙江省佳木斯市154000 佳木斯市中医医院影像科佳木斯市中医医院彩超室佳木斯大学附属口腔医院CT室佳木斯中心医院感染病科(

目的分析超声剪切波弹性成像(SWE)联合天冬氨酸氨基转移酶/丙氨酸氨基转移酶比值(AAR)、天冬氨酸氨基转移酶/血小板比值指数(APRI)和基于4因子的肝纤维化指标(FIB-4)检测诊断慢性乙型肝炎(CHB)患者肝纤维化程度的价值。方法2022年1月~2... 详细信息

目的分析超声剪切波弹性成像(SWE)联合天冬氨酸氨基转移酶/丙氨酸氨基转移酶比值(AAR)、天冬氨酸氨基转移酶/血小板比值指数(APRI)和基于4因子的肝纤维化指标(FIB-4)检测诊断慢性乙型肝炎(CHB)患者肝纤维化程度的价值。方法2022年1月~2024年6月我院诊治的CHB患者121例,均接受肝穿刺活检和SWE检查并测量杨氏模量值,常规行血液和血清检测,计算AAR、APRI和FIB-4。应用多因素Logistic回归分析影响CHB患者发生显著性肝纤维化的因素,应用受试者工作特征曲线(ROC)并计算曲线下面积(AUC)分析指标诊断显著性肝纤维化的效能。结果在121例CHB患者中,经肝组织病理学检查诊断肝纤维化F0期11例,F1期36例(非显著性肝纤维化47例),F2期40例,F3期19例(显著性肝纤维化59例)和F4期15例;显著组杨氏模量值、AAR、APRI和FIB-4分别为(11.5±3.1)kPa、(1.4±0.5)、(1.1±0.5)和(2.2±0.8),均显著大于非显著组[分别为(6.8±2.8)kPa、(1.0±0.3)、(0.6±0.2)和(1.5±0.6),P<0.05];多因素Logistic回归分析显示,杨氏模量值(OR=4.389,95%CI:2.059~9.352)、AAR(OR=2.342,95%CI:1.355~4.046)、APRI(OR=3.916,95%CI:1.892~8.102)和FIB-4(OR=1.306,95%CI:1.042~1.636)均是影响CHB患者发生显著性肝纤维化的独立危险因素;ROC曲线分析显示,应用杨氏模量值联合AAR、APRI和FIB-4诊断CHB患者显著性肝纤维化的AUC为0.950(95%CI:0.916~0.984),其敏感度为90.5%,特异度为80.9%,显著优于任一单一指标(P<0.05)。结论应用SWE联合AAR、APRI和FIB-4可帮助临床初步判断CHB患者肝纤维化程度,为进一步采取干预措施提供了线索。

关键词：慢性乙型肝炎肝纤维化超声剪切波弹性成像天冬氨酸氨基转移酶/丙氨酸氨基转移酶比值天冬氨酸氨基转移酶/血小板比值指数基于4因子的肝纤维化指数诊断

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Prevention of Mother-To-Child Transmission of HIV-China,2011-2020

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China CDC weekly 2021年第48期3卷 1018-1021页

作者： Xiaoyan wang Qian wang changhe wang Tong Zhang Zhixin Li Zhonghua Ma Ailing wang National Center for Women and Children’s Health China CDCBeijingChina Capital Institute of Pediatrics BeijingChina

ABSTRACT Introduction:Prevention of mother-to-child transmission(PMTCT)of human immunodeficiency virus(HIV)is a global health focus in most parts of the *** World Health Organization(WHO)launched the dual Elimination of Mother-to-Child Transmission(EMTCT)of HIV and syphilis initiative and released global guidance on criteria and processes for validation of the EMTCT in *** purpose of this report was to analyze the key validation indicators of EMTCT of HIV in China from 2011 to 2020 and to provide relevant basis information for the implementation of EMTCT action in China.

关键词： mother EMT HIV

Synaptotagmin-11 inhibits clathrin-mediated and bulk endocytosis in neurons

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Synaptotagmin-11 inhibits clathrin-mediated and bulk endocyt...

Synaptotagmin-11 inhibits clathrin-mediated and bulk endocytosis in neurons

中国生物化学与分子生物学会2016年全国学术会议

作者： changhe wang Center for Mitochondrial Biology and Medicine Frontier Institute of Science and Technology and The Key Laboratory of Biomedical Information Engineering of Ministry of Education School of Life Science and Technology Xi’an Jiaotong University

Precise and efficient endocytosis is essential for vesicle recycling during sustained *** regulation of neural endocytosis has been extensively studied and several exocytosis-related proteins,such as synaptotagmin-1 a...

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Synaptotagmin-11 inhibits clathrin-mediated and bulk endocyt...

STED Imaging of Vesicular Endocytosis in the Synapse

第十一届全国钙信号和细胞功能研讨会

Precise and efficient endocytosis is essential for vesicle recycling during sustained neurotransmission. The regulation of neural endocytosis has been extensively studied and several exocytosis-related proteins, such as synaptotagmin-1 and SNARE proteins, have been shown to promote exo-endocytosis for the efficient vesicle retrieval. However, inhibitors for endocytosis have rarely been found. Here, we show that synaptotagmin-11(Syt11), a non-Ca-binding Syt implicated in schizophrenia and Parkinson’s disease, inhibits clathrin-mediated endocytosis(CME) and bulk endocytosis in dorsal root ganglion neurons and hippocampal neurons. The frequency of both types of endocytic event increases in Syt11 knockdown neurons, while the sizes of endocytosed vesicles and the kinetics of individual bulk endocytotic events remain unaffected. Specifically, clathrin-coated pits and bulk endocytosis-like structures increase on the plasma membrane in Syt11-knockdown neurons. Structural-functional analysis reveals distinct domain requirements for Syt11 function in CME and bulk endocytosis. Importantly, the inhibitory role of Syt11 in endocytosis also slows down the vesicle pool replenishment and exocytosis recovery during the sustained neurotransmission. Taken together, we propose that Syt11 functions to ensure precision in vesicle retrieval, mainly by limiting the sites of membrane invagination at the early stage of endocytosis.

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Neuroscience Bulletin 2024年第9期40卷 1379-1395页

作者： Shaoqin Hu Zhenli Xie Bianbian wang Yang Chen Zexin Jing Ying Hao Jingyu Yao Xuanang Wu Jingxiao Huo Anqi Wei Yuhao Qin Nan Dong Chaowen Zheng Qian Song Jiangang Long Xinjiang Kang changhe wang Huadong Xu Neuroscience Research Center Key Laboratory of Biomedical Information Engineering of Ministry of EducationSchool of Life Science and TechnologyDepartment of Neurologythe First Affiliated HospitalCore Facilities Sharing PlatformXi’an Jiaotong UniversityXi’an710049China College of Life Sciences Liaocheng UniversityLiaocheng252059China Key Laboratory of Medical Electrophysiology Ministry of Education of ChinaCollaborative Innovation Center for Prevention and Treatment of Cardiovascular Diseaseand the Institute of Cardiovascular ResearchSouthwest Medical UniversityLuzhou646000China

Endocytosis is a fundamental biological process that couples exocytosis to maintain the homeostasis of the plasma membrane and sustained ***-resolution microscopy enables optical imaging of exocytosis and endocytosis in live cells and makes an essential contribution to understanding molecular mechanisms of endocytosis in neuronal somata and other types of ***,visualization of exo-endocytic events at the single vesicular level in a synapse with optical imaging remains a great challenge to reveal mechanisms governing the synaptic exo-endocytotic *** this protocol,we describe the technical details of stimulated emission depletion(STED)imaging of synaptic endocytosis at the single-vesicle level,from sample preparation and microscopy calibration to data acquisition and analysis.

关键词： Endocytosis STED imaging Synaptic vesicle Single-vesicle level

超高效液相色谱-串联质谱法测定蛇毒及蛇毒血凝酶制剂中38种药物残留

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中国药业 2025年第3期34卷 71-75页

作者：唐娜王建山宋莉田美虹王嫦鹤陕西省食品药品检验研究院陕西西安710065

目的建立测定蛇毒及蛇毒血凝酶制剂中38种药物残留量的超高效液相色谱-串联质谱法。方法采用电喷雾正/负离子和多反应监测模式扫描,以0.1%甲酸-0.5 mmol/L乙酸铵水溶液-乙腈为流动相(梯度洗脱),采用Waters Cortecs T3色谱柱(100 mm×2.1... 详细信息

目的建立测定蛇毒及蛇毒血凝酶制剂中38种药物残留量的超高效液相色谱-串联质谱法。方法采用电喷雾正/负离子和多反应监测模式扫描,以0.1%甲酸-0.5 mmol/L乙酸铵水溶液-乙腈为流动相(梯度洗脱),采用Waters Cortecs T3色谱柱(100 mm×2.1 mm,2.7μm)进行分离。结果38种药物在各自质量浓度范围内与峰面积线性关系良好(r>0.994,n=6);精密度、稳定性试验结果的RSD均小于4.00%(n=6);低、中、高质量浓度的加样回收率分别为78.34%~90.52%、83.61%~105.33%、88.21%~108.50%,RSD分别为2.51%~7.33%、0.84%~5.73%、1.21%~4.94%(n=3)。采用该方法检测了4批蛇毒血凝酶制剂和3种蛇毒中38种药物的残留情况,结果均未检出大环内酯类、磺胺类、喹诺酮类抗菌药物,符合相关要求,但在1批蛇毒中检出了17.2 ng/g氯霉素类抗菌药物氟甲砜霉素残留。结论该方法操作简便、重复性好、结果准确,适用于蛇毒及蛇毒血凝酶制剂中38种药物残留量的检测,可为蛇毒血凝酶类产品的质量控制提供参考。

关键词：超高效液相色谱-串联质谱法蛇毒蛇毒血凝酶制剂药物残留

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