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Enhanced osteoarthritis therapy by nanoengineered mesenchymal stem cells using biomimetic CuS nanoparticles loaded with plasmid DNA encoding TGF-β1

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Bioactive Materials 2023年第1期19卷 444-457页

作者： Yu Cai Cuixi Wu Qianhua Ou Muhui Zeng Song Xue Jieli Chen Yao Lu Changhai Ding Clinical Research Center Zhujiang HospitalSouthern Medical UniversityGuangzhouGuangdong510282China Orthopedic Center Zhujiang HospitalSouthern Medical UniversityGuangzhouGuangdong510282China Guangdong Key Lab of Orthopedic Technology and Implant GuangzhouGuangdong510010China Department of Rheumatology and Immunology Arthritis Research InstituteThe First Affiliated Hospital of Anhui Medical UniversityHefeiAnhui230032China Guangdong Provincial Key Laboratory of Bone and Joint Degeneration Diseases Academy of OrthopedicsSouthern Medical UniversityGuangzhouGuangdong510630China Menzies Institute for Medical Research University of TasmaniaHobartTasmania7000Australia

Mesenchymal stem cells(MSCs)therapy shows the potential benefits to relieve clinical symptoms of osteoarthritis(OA),but it is uncertain if it can repair articular cartilage lesions-the main pathology of OA.Here,we prepared biomimetic cupper sulfide@phosphatidylcholine(CuS@PC)nanoparticles(NPs)loaded with plasmid DNA(pDNA)encoding transforming growth factor-beta 1(TGF-β1)to engineer MSCs for enhanced OA therapy via cartilage regeneration.We found that the NPs not only promoted cell proliferation and migration,but also presented a higher pDNA transfection efficiency relative to commercial transfection reagent lipofectamine 3000.The resultant CuS/TGF-β1@PC NP-engineered MSCs(termed CTP-MSCs)were better than pure MSCs in terms of chondrogenic gene expression,glycosaminoglycan deposition and type II collagen formation,favoring cartilage repair.Further,CTP-MSCs inhibited extracellular matrix degradation in interleukin-1β-induced chondrocytes.Consequently,intraarticular administration of CTP-MSCs significantly enhanced the repair of damaged cartilage,whereas pure MSCs exhibited very limited effects on cartilage regeneration in destabilization of the medial meniscus(DMM)surgical instability mice.Hence,this work provides a new strategy to overcome the limitation of current stem cell therapy in OA treatment through developing more effective nanoengineered MSCs.

关键词： Osteoarthritis Stem cell therapy Cartilage regeneration Cupper sulfide nanoparticles Gene delivery

Preliminary experimental results by the prototype of Sanya Incoherent Scatter Radar

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Earth and Planetary Physics 2020年第6期4卷 579-587页

作者： XinAn Yue WeiXing Wan Han Xiao LingQi Zeng changhai Ke BaiQi Ning Feng ding BiQiang Zhao Lin Jin Chen Li MingYuan Li JunYi Wang HongLian Hao Ning Zhang Key Laboratory of Earth and Planetary Physics Institute of Geology and GeophysicsChinese Academy of SciencesBeijing 100029China Innovation Academy for Earth Science Chinese Academy of SciencesBeijing 100029China Beijing National Observatory of Space Environment Institute of Geology and GeophysicsChinese Academy of SciencesBeijing 100029China College of Earth and Planetary Sciences University of the Chinese Academy of SciencesBeijing 100049China Nanjing Research Institute of Electronics Technology Nanjing 210039China

In the past decades,the Incoherent Scatter Radar(ISR)has been demonstrated to be one of the most powerful instruments for ionosphere monitoring.The Institute of Geology and Geophysics at the Chinese Academy of Sciences was founded to build a state-ofthe-art phased-array ISR at Sanya(18.3°N,109.6°E),a low-latitude station on Hainan Island,named the Sanya ISR(SYISR).As a first step,a prototype radar system consisting of eight subarrays(SYISR-8)was built to reduce the technical risk of producing the entire large array.In this work,we have summarized the preliminary experimental results based on the SYISR-8.The amplitude and phase among 256 channels were first calibrated through an embedded internal monitoring network.The mean oscillation of the amplitude and phase after calibration were about 1 dB and 5°,respectively,which met the basic requirements.The beam directivity was confirmed by crossing screen of the International Space Station.The SYISR-8 was further used to detect the tropospheric wind profile and meteors.The derived winds were evaluated by comparison with independent radiosonde and balloon-based GPS measurements.The SYISR-8 was able to observe several typical meteor echoes,such as the meteor head echo,range-spread trail echo,and specular trail echo.These results confirmed the validity and reliability of the SYISR-8 system,thereby reducing the technical risk of producing the entire large array of the SYISR to some extent.

关键词： incoherent scatter radar SYISR ionosphere phased array beam direction tropospheric wind meteor

溴代甲基咪唑修饰的蜜勒胺低聚物催化CO_(2)环加成反应性能

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精细化工 2023年第6期40卷 1376-1385页

作者：岳昌海黄益平孙玉玉黄晶晶丁辉丁靖中建安装集团有限公司江苏南京210023 南京工业大学化工学院江苏南京211816

采用两步法合成了溴代甲基咪唑修饰的蜜勒胺低聚物(CN-MIm),利用FTIR、XRD、SEM、TG和XPS对其结构、形貌和热稳定性进行了表征。考察了反应工艺条件对环氧氯丙烷(ECH)和CO_(2)环加成反应的影响。结果表明,咪唑基离子液体成功负载在蜜勒... 详细信息

采用两步法合成了溴代甲基咪唑修饰的蜜勒胺低聚物(CN-MIm),利用FTIR、XRD、SEM、TG和XPS对其结构、形貌和热稳定性进行了表征。考察了反应工艺条件对环氧氯丙烷(ECH)和CO_(2)环加成反应的影响。结果表明,咪唑基离子液体成功负载在蜜勒胺低聚物上,且具有较好的热稳定性。以CN-MIm为催化剂,在反应温度为120℃、反应时间为1.5 h,CO_(2)压力为2.0 MPa、催化剂(0.1 g)与ECH质量比为0.036∶1条件下,ECH转化率和氯代碳酸丙烯酯产率分别为99%和98%。CN-MIm对不同环氧化物均具有良好的普适性。此外,提出了蜜勒胺低聚物中含有的羟基、氨基及咪唑基离子液体中Br–对环氧化物和CO_(2)环加成的协同催化反应机理。

关键词：蜜勒胺低聚物离子液体溴代甲基咪唑 CO_(2)环加成精细化工中间体

准噶尔盆地西北缘不整合储层流体包裹体特征与油气成藏期次

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天然气工业 2015年第11期35卷 23-32页

作者：高长海查明曲江秀丁修建中国石油大学(华东)地球科学与技术学院

准噶尔盆地西北缘存在着众多的地层油气藏,目前对于其油气成藏期次及成藏时间的认识尚存在争议。为此,采集了该区26口钻井的58块流体包裹体样品,利用流体包裹体显微测温技术,结合埋藏史及热演化史分析成果,研究了上述流体包裹体的显微... 详细信息

准噶尔盆地西北缘存在着众多的地层油气藏,目前对于其油气成藏期次及成藏时间的认识尚存在争议。为此,采集了该区26口钻井的58块流体包裹体样品,利用流体包裹体显微测温技术,结合埋藏史及热演化史分析成果,研究了上述流体包裹体的显微特征和均一温度特征,确定了该区地层油气藏的油气成藏期次,进一步结合该区烃源岩生排烃史和构造发育史,恢复了地层油气藏的成藏过程。结论认为:①晚二叠世-早三叠世,二叠系佳木河组烃源岩生成的成熟油气进入石炭系火山岩储层和二叠系佳木河组、风城组、夏子街组等碎屑岩不整合储层中聚集成藏;②晓三叠世-早侏罗世,二叠系风城组烃源岩生成的成熟油气进入二叠系内部以及三叠系底部不整合砂砾岩储层中聚集,以形成中深部油藏为主;③晚侏罗世-早白垩世,二叠系佳木河组烃源岩生成的天然气和二叠系下乌尔禾组烃源岩生成的成熟油气进入三叠系底部、侏罗系底部、白垩系底部等碎屑岩不整合储层中聚集,以形成中浅部油/气藏和稠油油藏为主。

关键词：准噶尔盆地西北缘不整合面储集层流体包裹体成藏期次成藏过程热演化史烃源岩

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网络拓扑结构对网络安全的影响

信息通信技术与政策 2019年第2期 57-60页

作者：胡长海丁亮亮中国联合网络通信有限公司西安市分公司运行维护部

网络拓扑结构是在组网时各个设备的连通形式,通常包括总线型、环形、星型等结构。本文通过对网络安全,尤其是运营商侧的网络安全概念的提出,结合各个网络拓扑结构的优缺点,给出了最适合现行城域网的网络拓扑结构,并在最后给出了对现网... 详细信息

网络拓扑结构是在组网时各个设备的连通形式,通常包括总线型、环形、星型等结构。本文通过对网络安全,尤其是运营商侧的网络安全概念的提出,结合各个网络拓扑结构的优缺点,给出了最适合现行城域网的网络拓扑结构,并在最后给出了对现网进行环改星网络优化的一种方案。

关键词：网络安全拓扑星型网络

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临床研究中的多重检验

广州医药 2023年第1期54卷 11-15页

作者：区倩华阮光峰丁长海中山市人民医院重症医学科中山528403 广州市第一人民医院临床研究中心广州510180 南方医科大学珠江医院临床研究中心广州510280

临床研究中,我们会经常遇到多重检验的问题。当同时检验多个假设时,如何控制犯Ⅰ类错误的概率,保证结果的准确性,是研究者面对的首要问题。然而未对多重检验进行校正的文章比例仍较大。本文从简单的单个检验假设出发,回答为什么需要进... 详细信息

临床研究中,我们会经常遇到多重检验的问题。当同时检验多个假设时,如何控制犯Ⅰ类错误的概率,保证结果的准确性,是研究者面对的首要问题。然而未对多重检验进行校正的文章比例仍较大。本文从简单的单个检验假设出发,回答为什么需要进行多重校正,何时进行多重校正,同时介绍总体错误率(FWER)和错误发现率(FDR)两种重要的错误率以及在此基础上的Bonferroni和Benjamini-Hochberg校正方法,从而避免因多重检验问题带来的混乱。

关键词：多重检验多重校正总体错误率错误发现率

Modelling of low-temperature exhumation rate in Dabie Mountain based on (U-Th)/He and fission-track thermochronological data

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Science China Earth Sciences 2006年第10期49卷 1009-1019页

作者： ding Ruxin ZHOU Zuyi XU changhai LIU Yuzhu CHENG Hao XU Fei State Key Laboratory of Marine Geology Tongji UniversityShanghai 200092China State Key Laboratory of Marine GeologyTongji UniversityShanghai 200092China State Key Laboratory of Marine GeologyTongji UniversityShanghai 200092China State Key Laboratory of Marine GeologyTongji UniversityShanghai 200092China State Key Laboratory of Marine GeologyTongji UniversityShanghai 200092China State Key Laboratory of Marine GeologyTongji UniversityShanghai 200092China

While the high-temperature exhumation process in the Dabie Mountain has been well documented, the low-temperature exhumation of this area since Cretaceous, especially since Late Cretaceous, is relatively less studied. Low-temperature thermochronology provides one of the important approaches to solve this problem. Based on the data of fission track and (U-Th)/He analysis of apaptites and zircons from the granitoid and metamorphic rocks in the Dabie Mountain, this paper applies Mancktelow’s and Braun’s methods to estimating the exhumation rates and to drawing the regional differential exhumation pattern since Cretaceous, especially since Late Cretaceous by taking into consideration factors such as heat transport, heat advection, topography and heat production, which could influence geothermal field in the shallow crust. Since Cretaceous, the exhumation rate (0.08-0.10 km/Ma) in the region around Tiantangzhai and in the south of Tanlu fault zone is larger than the rate (0.04-0.07 km/Ma) in other areas of the Dabie Mountain. The regional differential exhumation pattern might be related to the push-up effect caused by differential strike-slip movement along NNE-trending faults.

关键词： Dabie Mountain, fission track, (U-Th)/He, modelling, exhumation.

膝骨关节炎患者髌上囊渗液量与疼痛及血清白细胞介素-6-17-23相关性研究

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中华风湿病学杂志 2015年第4期19卷 219-223页

作者：杨雪晴徐建华丁长海郑双安徽医科大学第一附属医院风湿免疫科合肥230022

目的 MRI测定膝OA髌上囊渗液量,探讨其与疼痛及炎症因子（IL-6、IL-17、IL-23）的关系.方法选取膝OA患者204例.拍摄膝关节MRI片,用OSIRIS软件测定膝关节髌上囊渗液,包括渗液量的等级（0～3级）和渗液最大面积（cm2）.测定血清IL-6、IL... 详细信息

目的 MRI测定膝OA髌上囊渗液量,探讨其与疼痛及炎症因子（IL-6、IL-17、IL-23）的关系.方法选取膝OA患者204例.拍摄膝关节MRI片,用OSIRIS软件测定膝关节髌上囊渗液,包括渗液量的等级（0～3级）和渗液最大面积（cm2）.测定血清IL-6、IL-17及IL-23水平.问卷评定患者关节疼痛症状,包括WOMAC评分及Lequesne指数,详细记录患者的一般情况,计算BMI等.应用t检验,x2检验,多元线性回归分析或Logistic回归进行分析.结果 ①204例患者,年龄（55±8）岁,其中女性176例（86.3％）.生理性渗液（0～1级）83例（47.9％）,病理性渗液（2～3级）90例（52.1％）.②与低等级渗液量组相比,高等级渗液量组患者的年龄、体力劳动强度、血尿酸及Lequesne指数均升高（P均＜0.05）.③髌上囊渗液最大面积与患者年龄呈正相关（r=0.208,P=0.006）,与BMI呈正相关（r=0.171,P=0.027）,与体力劳动强度呈正相关（r=0.189,P=0.013）.④Logistic回归分析示排除性别、年龄、BMI的影响后发现,髌上囊渗液等级与Lequesne指数呈正相关[OR=1.120,95％ CI（1.011,1.241）,P=0.029],与IL-23呈负相关[OR=0.768,95％CI（0.598,0.986）,P=0.038],与WOMAC评分、IL-6、IL-17无显著相关性.结论膝OA患者中病理性渗液发生率高达52.1％.髌上囊渗液量与疼痛呈正相关,与IL-23呈负相关.

关键词：骨关节炎膝渗液疼痛白细胞介素23

吉木萨尔凹陷芦草沟组超高压形成机制及演化特征

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新疆石油地质 2016年第6期37卷 637-643页

作者：赖仁查明高长海曲江秀丁修建中国石油大学地球科学与技术学院山东青岛266580

准噶尔盆地吉木萨尔凹陷中二叠统芦草沟组普遍发育超高压, 综合运用测井、钻井、试油资料及盆地模拟技术, 分析了现今地层压力特征和超高压形成机制, 并恢复了芦草沟组古压力演化特征.研究结果表明, 吉木萨尔凹陷现今压力结构由浅至... 详细信息

准噶尔盆地吉木萨尔凹陷中二叠统芦草沟组普遍发育超高压, 综合运用测井、钻井、试油资料及盆地模拟技术, 分析了现今地层压力特征和超高压形成机制, 并恢复了芦草沟组古压力演化特征.研究结果表明, 吉木萨尔凹陷现今压力结构由浅至深可分为常压带、浅层超高压带、压力过渡带和深层超高压带4个压力带; 芦草沟组位于深层超高压带, 在横向上超高压具有一定的连续性, 剩余压力多为10-20 MPa, 压力系数为1.10-1.50. 芦草沟组地层压力演化经历了3个半 “增压-泄压” 旋回, 且压力演化的旋回性与沉积速率、生烃排烃和构造抬升存在较好的匹配关系; 沉积物快速埋藏导致的欠压实是形成第一期超高压的主要原因, 在三叠纪晚期进入生烃门限后, 生烃增压则成为形成第二期和第三期超高压的重要机理.

关键词：准噶尔盆地吉木萨尔凹陷芦草沟组盆地模拟古压力压力系数超压成因机制