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Highly expressed BMP9/GDF2 in postnatal mouse liver and lungs may account for its pleiotropic effects on stem cell differentiation,angiogenesis,tumor growth and metabolism

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Genes & Diseases 2020年第2期7卷 235-244页

作者： Wei Liu Zhongliang Deng Zongyue Zeng Jiaming Fan Yixiao Feng Xi Wang Daigui Cao Bo Zhang Lijuan Yang Bin Liu Mikhail Pakvasa William Wagstaff Xiaoxing Wu Huaxiu Luo Jing Zhang Meng Zhang Fang He Yukun Mao Huiming Ding Yongtao Zhang changchun niu Rex C.Haydon Hue H.Luu Jennifer Moriatis Wolf Michael J.Lee Wei Huang Tong-Chuan He Yulong Zou Departments of Orthopedic Surgery Breast SurgeryGastrointestinal SurgeryObstetrics and Gynecologyand NephrologyThe First Affiliated Hospital of Chongqing Medical UniversityChongqing 400016China Molecular Oncology Laboratory Department of Orthopaedic Surgery and Rehabilitation MedicineThe University of Chicago Medical CenterChicagoIL 60637USA Department of Orthopaedic Surgery The Second Affiliated Hospital of Chongqing Medical UniversityChongqing 400010China Ministry of Education Key Laboratory of Diagnostic Medicine The School of Laboratory MedicineChongqing Medical UniversityChongqing 400016China Departments of Orthopaedic Surgery and Laboratory Diagnostic Medicine Chongqing General HospitalChongqing 400021China Key Laboratory of Orthopaedic Surgery of Gansu Province The Departments of Orthopaedic Surgery and Obstetrics and GynecologyThe First and Second Hospitals of Lanzhou UniversityLanzhou730030China School of Life Sciences Southwest UniversityChongqing 400715China Department of Burn and Plastic Surgery West China Hospital of Sichuan UniversityChengdu 610041China Department of Orthopaedic Surgery The First Affiliated Hospital of Guangzhou University of Chinese MedicineGuangzhou 510405China Department of Orthopaedic Surgery The Affiliated Zhongnan Hospital of Wuhan UniversityWuhan 430072China Department of Orthopaedic Surgery BenQ Medical Center Affiliated with Nanjing Medical UniversityNanjing 210000China Department of Orthopaedic Surgery The Affiliated Hospital of Qingdao UniversityQingdao 266061China

Bone morphogenetic protein 9(BMP9)(or GDF2)was originally identified from fetal mouse liver cDNA *** evidence indicates BMP9 exerts diverse and pleiotropic functions during postnatal development and in maintaining tissue ***,the expression landscape of BMP9 signaling during development and/or in adult tissues remains to be ***,we conducted a comprehensive analysis of the expression landscape of BMP9 and its signaling mediators in postnatal *** analyzing mouse ENCODE transcriptome datasets we found Bmp9 was highly expressed in the liver and detectable in embryonic brain,adult lungs and adult *** next conducted a comprehensive qPCR analysis of RNAs isolated from major mouse tissues/organs at various *** found that Bmp9 was highly expressed in the liver and lung tissues of young adult mice,but decreased in older ***,Bmp9 was only expressed at low to modest levels in developing ***9-associated TGFβ/BMPR type I receptor Alk1 was highly expressed in the adult ***,the feedback inhibitor Smads Smad6 and Smad7 were widely expressed in mouse postnatal ***,the BMP signaling antagonist noggin was highly expressed in fat and heart in the older age groups,as well as in kidney,liver and lungs in a biphasic ***,our findings indicate that the circulating BMP9 produced in liver and lungs may account for its pleiotropic effects on postnatal tissues/organs although possible roles of BMP9 signaling in liver and lungs remain to be fully understood.

关键词： BMP9/GDF2 Bone morphogenetic proteins(BMPs) Hepatic metabolism Mesenchymal stem cells Neurogenesis Osteogenic differentiation Pulmonary arterial hypertension Tumorigenesis

Genetically modified pigs with CD163 point mutation are resistant to HP-PRRSV infection

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Zoological Research 2024年第4期45卷 833-844页

作者： Ying Liu Lin Yang Hong-Yong Xiang Ming niu Jia-Cheng Deng Xue-Yuan Li Wen-Jie Hao Hong-Sheng Ou-Yang Tong-Yu Liu Xiao-Chun Tang Da-Xin Pang Hong-Ming Yuan State Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases Animal Genome Editing Technology Innovation CenterCollege of Animal SciencesJilin UniversityChangchunJilin 130062China Chifeng Best Genetics Technology Co. Ltd.ChifengNei Mongol 024000China Chongqing Jitang Biotechnology Research Institute Co. Ltd.Chongqing 400015China

Porcine reproductive and respiratory syndrome(PRRS)is a globally prevalent contagious disease caused by the positive-strand RNA PRRS virus(PRRSV),resulting in substantial economic losses in the swine *** the CD163 SRCR5 domain,either through deletion or substitution,can eff1ectively confer resistance to PRRSV infection in ***,large fragment modifications in pigs inevitably raise concerns about potential adverse effects on growth *** the impact of genetic modifications on normal physiological functions is a promising direction for developing PRRSV-resistant *** the current study,we identified a specific functional amino acid in CD163 that influences PRRSV *** infection experiments conducted on Marc145 and PK-15CD163 cells illustrated that the mE535G or corresponding pE529G mutations markedly inhibited highly pathogenic PRRSV(HP-PRRSV)proliferation by preventing viral binding and ***,individual viral challenge tests revealed that pigs with the E529G mutation had viral loads two orders of magnitude lower than wild-type(WT)pigs,confirming effective resistance to *** of the physiological indicators and scavenger function of CD163 verified no significant differences between the WT and E529G *** findings suggest that E529G pigs can be used for breeding PRRSV-resistant pigs,providing novel insights into controlling future PRRSV outbreaks.

关键词： PRRSV CD163 Point mutation E529G Pigs

重庆市2011-2020年临床微生物室间质量评价运行总结分析

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国际检验医学杂志 2022年第5期43卷 615-617,621页

作者：王淑玲张震徐兰兰颜令牛长春李甜廖璞中国科学院大学重庆医院/重庆市人民医院检验科/重庆市临床检验中心重庆400014

目的分析重庆市2011-2020年临床微生物项目室间质量评价运行情况,为提高该市临床微生物鉴定和药物灵敏度检测能力提供依据。方法收集2011-2020年参加该市临床检验中心临床微生物项目室间质量评价实验室结果,对结果进行统计分析。结果201... 详细信息

目的分析重庆市2011-2020年临床微生物项目室间质量评价运行情况,为提高该市临床微生物鉴定和药物灵敏度检测能力提供依据。方法收集2011-2020年参加该市临床检验中心临床微生物项目室间质量评价实验室结果,对结果进行统计分析。结果2011-2020年参加的实验室从93家发展至138家,合格率从61.3%到94.2%。10年共发放100株细菌,涉及35个菌属,55个菌种。细菌鉴定和药物灵敏度正确率逐年增高,少见菌鉴定和一些药物灵敏度检测仍存在一些问题。结论通过临床微生物室间质量评价,发现实验室检测的不足,持续改进,提高实验室的检测能力,为临床诊疗提供可靠的依据。

关键词：室间质量评价细菌鉴定药物灵敏度试验

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“积极保护”理念与济南老城保护规划

规划师 2012年第8期28卷 84-87页

作者：王新文牛长春张中堃王洪梅济南市济南市规划局城乡规划处济南市城乡规划编制研究中心

进入21世纪,济南市城市化进程不断加快,保护与发展的矛盾日益突出。如何处理老城保护与经济发展的关系,如何在保护特色风貌的同时实现老城的现代化,成为急需解决的问题。济南市通过对老城保护存在问题的分析,阐述了基于规划管理角度的"... 详细信息

进入21世纪,济南市城市化进程不断加快,保护与发展的矛盾日益突出。如何处理老城保护与经济发展的关系,如何在保护特色风貌的同时实现老城的现代化,成为急需解决的问题。济南市通过对老城保护存在问题的分析,阐述了基于规划管理角度的"积极保护"理念内容,即注重保护的科学、统筹、整体、重点、有机、特色六个方面,并编制了《济南商埠风貌区规划》等规划,在实践层面进行了探索,提出了相应的实施建议。

关键词：积极保护老城保护济南

Notch signaling:Its essential roles in bone and craniofacial development

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Genes & Diseases 2021年第1期8卷 8-24页

作者： Mikhail Pakvasa Pranav Haravu Michael Boachie-Mensah Alonzo Jones Elam Coalson Junyi Liao Zongyue Zeng Di Wu Kevin Qin Xiaoxing Wu Huaxiu Luo Jing Zhang Meng Zhang Fang He Yukun Mao Yongtao Zhang changchun niu Meng Wu Xia Zhao Hao Wang Linjuan Huang Deyao Shi Qing Liu Na Ni Kai Fu Michael J.Lee Jennifer Moriatis Wolf Aravind Athiviraham Sherwin S.Ho Tong-Chuan He Kelly Hynes Jason Strelzow Mostafa El Dafrawy Russell R.Reid Molecular Oncology Laboratory Department of Orthopaedic Surgery and Rehabilitation MedicineThe University of Chicago Medical CenterChicagoIL 60637USA Pritzker School of Medicine University of ChicagoChicagoIL 60637USA Section of Plastic and Reconstructive Surgery Department of SurgeryThe University of Chicago Medical CenterChicagoIL 60637USA Departments of Orthopaedic Surgery Gastrointestinal SurgeryObstetrics and Gynecologyand NephrologyThe First Affiliated Hospital of Chongqing Medical UniversityChongqing400016PR China Ministry of Education Key Laboratory of Diagnostic Medicine and School of Laboratory and Diagnostic MedicineChongqing Medical UniversityChongqing400016PR China Department of Burn and Plastic Surgery West China Hospital of Sichuan UniversityChengduSichuan610041PR China Department of Orthopaedic Surgery The First Affiliated HospitalGuangzhou University of Chinese MedicineGuangzhouGuangdong510405PR China Departments of Orthopaedic Surgery and Neurosurgery The Affiliated Zhongnan Hospital of Wuhan UniversityWuhanHubei430072PR China Department of Orthopaedic Surgery The Affiliated Hospital of Qingdao UniversityQingdaoShandong266061PR China Department of Laboratory Diagnostic Medicine Chongqing General HospitalChongqing400021PR China Institute of Bone and Joint Research and the Department of Orthopaedic SurgeryThe Second Hospitals of Lanzhou UniversityGansuLanzhou730030PR China Department of Orthopaedics Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhanHubei430072PR China Department of Spine Surgery Second Xiangya HospitalCentral South UniversityChangshaHunan410011PR China

Notch is a cellecell signaling pathway that is involved in a host of activities including development,oncogenesis,skeletal homeostasis,and much *** specifically,recent research has demonstrated the importance of Notch signaling in osteogenic differentiation,bone healing,and in the development of the *** craniofacial skeleton is complex and understanding its development has remained an important focus in *** this review we briefly summarize what recent research has revealed about Notch signaling and the current understanding of how the skeleton,skull,and face *** then discuss the crucial role that Notch plays in both craniofacial development and the skeletal system,and what importance it may play in the future.

关键词： Alagille syndrome Bone Craniofacial development Craniosynostosis Notch Oncogenesis Osteogenesis Spondylocostal dysosotosis

Newcastle disease virus suppresses antigen presentation via inhibiting IL-12 expression in dendritic cells

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2024年第3期25卷 254-270页

作者： Fulong NAN Wenlong NAN Xin YAN Hui WANG Shasha JIANG Shuyun ZHANG Zhongjie YU Xianjuan ZHANG Fengjun LIU Jun LI Xiaoqiong ZHOU Delei niu Yiquan LI Wei WANG Ning SHI Ningyi JIN Changzhan XIE Xiaoni CUI He ZHANG Bin WANG Huijun LU Department of Special Medicine Department of Pathogenic BiologySchool of Basic MedicineQingdao UniversityQingdao 266071China China Animal Health and Epidemiology Center Qingdao 266032China Academician Workstation of Jilin Province Changchun University of Chinese MedicineChangchun 130117China Institute of Virology Wenzhou UniversityWenzhou 325035China College of Veterinary Medicine Jilin UniversityChangchun 130012China changchun Veterinary Research Institute Chinese Academy of Agricultural SciencesChangchun 130122China Sino-Cell Biomed Co. Ltd.Qingdao 266000China

As a potential vectored vaccine,Newcastle disease virus(NDV)has been subject to various studies for vaccine development,while relatively little research has outlined the immunomodulatory effect of the virus in antigen *** elucidate the key inhibitory factor in regulating the interaction of infected dendritic cells(DCs)and T cells,DCs were pretreated with the NDV vaccine strain LaSota as an inhibitor and stimulated with lipopolysaccharide(LPS)for further detection by enzyme-linked immunosorbent assay(ELISA),flow cytometry,immunoblotting,and quantitative real-time polymerase chain reaction(qRT-PCR).The results revealed that NDV infection resulted in the inhibition of interleukin(IL)-12p40 in DCs through a p38 mitogen-activated protein kinase(MAPK)-dependent manner,thus inhibiting the synthesis of IL-12p70,leading to the reduction in T cell proliferation and the secretion of interferon-(IFN-),tumor necrosis factor-α(TNF-α),and IL-6 induced by ***,downregulated cytokines accelerated the infection and viral transmission from DCs to T ***,several other strains of NDV also exhibited inhibitory *** current study reveals that NDV can modulate the intensity of the innate-adaptive immune cell crosstalk critically toward viral invasion improvement,highlighting a novel mechanism of virus-induced immunosuppression and providing new perspectives on the improvement of NDV-vectored vaccine.

关键词： Newcastle disease virus Dendritic cells Interleukin-12(IL-12) T cells Immunosuppression

探讨山东省“社会主义新农村”建设中的节能策略

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规划师 2007年第B4期23卷 26-28页

作者：李沛牛长春耿谦李强济南市规划设计研究院济南市城乡规划编制研究中心山东三箭建筑工程股份有限公司

在“社会主义新农村”建设中探索生态建筑节能策略,有效改善农村建筑的居住舒适度,是新农村建设成功的关键。山东省属于寒冷地区,充分利用太阳能是节约能源的有效途径。规划应根据气候特点,通过合理的基地选择、场地设计、建筑物朝向选... 详细信息

在“社会主义新农村”建设中探索生态建筑节能策略,有效改善农村建筑的居住舒适度,是新农村建设成功的关键。山东省属于寒冷地区,充分利用太阳能是节约能源的有效途径。规划应根据气候特点,通过合理的基地选择、场地设计、建筑物朝向选择、日照间距设计等,在冬季尽量争取日照,在夏季改善建筑的微气候。建筑设计则应注意建筑平面、建筑体形等方面。

关键词：社会主义新农村节能策略村镇规划建筑设计

微光CMOS传感器调制传递函数快速测试方法

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激光与光电子学进展 2024年第12期61卷 287-293页

作者：唐卓王劲松牛群刘小华长春理工大学光电工程学院吉林长春130022 长春理工大学中山研究院广东中山528437

为满足微光CMOS图像传感器调制传递函数快速检测的需求,通过优化倾斜刃边法提出一种基于超采样模型的调制传递函数快速测试方法。测试过程采用自适应双阈值Canny边缘检测算法提高刃边检测精度,通过刃边角度空间几何旋转对边缘扩展响应... 详细信息

为满足微光CMOS图像传感器调制传递函数快速检测的需求,通过优化倾斜刃边法提出一种基于超采样模型的调制传递函数快速测试方法。测试过程采用自适应双阈值Canny边缘检测算法提高刃边检测精度,通过刃边角度空间几何旋转对边缘扩展响应进行超采样,并利用三次费米函数等效模型进行拟合,极大消除自然微光成像中噪声对测试结果的影响。利用该方法对CMOS传感器进行实际测试,采用不同目标图片测试实验结果同标准量进行对比,误差均在0.06之内,证明了该方法的可行性与有效性,符合CMOS传感器性能检测的要求。

关键词：调制传递函数微光传感器倾斜刃边法边缘超采样快速检测

Asymptotic Distribution of the Jump Change-Point Estimator

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Chinese Annals of Mathematics,Series B 2012年第3期33卷 429-436页

作者： changchun TAN Huifang niu Baiqi MIAO School of Mathematics Hefei University of TechnologyHefei 230009China College of Mathematics Luoyang Normal UniversityLuoyang 471022HenanChina Department of Statistics and Finance University of Science and Technology of ChinaHefei 230026China

The asymptotic distribution of the change-point estimator in a jump change- point model is considered. For the jump change-point model Xi - α ＋ θ{[nτ0] 〈 i ≤ n} ＋ εi, where εi （i = 1,…. , n） are independent identically distributed random variables with Eεi -= 0 and Var（εi）〈 ∞, with the help of the slip window method, the asymptotic distribution of the jump change-point estimator τ is studied under the condition of the local alternative hypothesis.

关键词： Change-point Local alternative hypothesis Asymptotic distribution