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济南市大型公共设施规划与实施初探

城市规划 2007年第4期31卷 71-73,81页

作者：牛长春李沛李文彬济南市城乡规划编制研究中心规划编制所济南市规划设计研究院

随着我国经济体制的转轨,在政府和各个部门缺乏统一政策的情况下,城市大型公共设施的规划和实施产生了一系列问题,严重影响了大型公共设施的建设。本文通过对济南市大型公共设施的现状、规划与实施的分析和探讨,有针对性地提出了大型公... 详细信息

随着我国经济体制的转轨,在政府和各个部门缺乏统一政策的情况下,城市大型公共设施的规划和实施产生了一系列问题,严重影响了大型公共设施的建设。本文通过对济南市大型公共设施的现状、规划与实施的分析和探讨,有针对性地提出了大型公共设施规划和实施的建议。

关键词：济南市公益性大型公共设施

Development of a simplified and inexpensive RNA depletion method for plasmid DNA purification using size selection magnetic beads(SSMBs)

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Genes & Diseases 2021年第3期8卷 298-306页

作者： Xi Wang Ling Zhao Xiaoxing Wu Huaxiu Luo Di Wu Meng Zhan Jing Zhang Mikhail Pakvasa William Wagstaff Fang He Yukun Mao Yongtao Zhang changchun niu Meng Wu Xia Zhao Hao Wang Linjuan Huang Deyao Shi Qing Liu Na Ni Kai Fu Kelly Hynes Jason Strelzow Mostafa El Dafrawy Tong-Chuan He Hongbo Qi Zongyue Zeng Ministry of Education Key Laboratory of Diagnostic Medicine School of Laboratory and Diagnostic MedicineChongqing Medical UniversityChongqing400016PR China Molecular Oncology Laboratory Department of Orthopaedic Surgery and Rehabilitation MedicineThe University of Chicago Medical CenterChicagoIL60637USA Departments of Gastrointestinal Surgery Obstetrics and Gynecologyand NephrologyThe First Affiliated Hospital of Chongqing Medical UniversityChongqing400016PR China Department of Burn and Plastic Surgery West China Hospital of Sichuan UniversityChengduSichuan Province610041PR China Department of Orthopaedic Surgery The First Affiliated HospitalGuangzhou University of Chinese MedicineGuangzhouGuangdong Province510405PR China Departments of Orthopaedic Surgery and Neurosurgery The Affiliated Zhongnan Hospital of Wuhan UniversityWuhanHubei Province430072PR China Department of Orthopaedic Surgery The Affiliated Hospital of Qingdao UniversityQingdaoShandong Province266061PR China Department of Laboratory Diagnostic Medicine Chongqing General HospitalChongqing400021PR China Institute of Boneand Joint Research The departments of Orthopaedic Surgery and Obstetrics and GynecologyThe First and Second Hospitals of Lanzhou UniversityLanzhouGansuProvince730030PR China Department of Orthopaedics Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhanHubei Province430022PR China Department of Spine Surgery Second Xiangya HospitalCentral South UniversityChangshaHunan Province410011PR China

Plasmid DNA(pDNA)isolation from bacterial cells is one of the most common and critical steps in molecular cloning and biomedical *** all pDNA purification in-volves disruption of bacteria,removal of membrane lipids,proteins and genomic DNA,purifi-cation of pDNA from bulk lysate,and concentration of pDNA for downstream *** many liquid-phase and solid-phase pDNA purification methods are used,the final pDNA preparations are usually contaminated with varied degrees of host RNA,which cannot be completely digested by RNase *** develop a simple,cost-effective,and yet effective method for RNA depletion,we investigated whether commercially available size selection magnetic beads(SSMBs),such as Mag-Bind®TotalPure NGS Kit(or Mag-Bind),can completely deplete bacterial RNA in pDNA *** this proof-of-principle study,we demonstrated that,compared with RNase A digestion and two commercial plasmid affinity purification kits,the SSMB method was highly efficient in depleting contaminating RNA from pDNA *** transfection and bacterial colony formation assays revealed that pDNA purified from SSMB method had superior quality and integrity to pDNA samples cleaned up by RNase A digestion and/or commercial plasmid purification *** further demonstrated that the SSMB method completely depleted contaminating RNA in large-scale pDNA ***,the Mag-bind-based SSMB method costs only 5-10%of most commercial plasmid purification kits on a per sample ***,the reported SSMB method can be a valuable and inexpensive tool for the removal of bacterial RNA for routine pDNA preparations.

关键词： DNA transfection DNA vaccination Gene delivery Plasmid DNA purification RNA depletion Size selection magnetic beads

Modeling colorectal tumorigenesis using the organoids derived from conditionally immortalized mouse intestinal crypt cells (ciMICs)

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Genes & Diseases 2021年第6期8卷 814-826页

作者： Xiaoxing Wu Zhaoxia Li Hongyu Zhang Fang He Min Qiao Huaxiu Luo Jing Zhang Meng Zhang Yukun Mao William Wagstaff Yongtao Zhang changchun niu Xia Zhao Hao Wang Linjuan Huang Deyao Shi Qing Liu Na Ni Kai Fu Rex C.Haydon Russell R.Reid Hue H.Luu Tong-Chuan He Ziwei Wang Houjie Liang Bing-Qiang Zhang Ning Wang Departments of Gastrointestinal Surgery Medicine/Gastroenterologyand Obstetrics and GynecologyThe First Affiliated Hospital of Chongqing Medical UniversityChongqing400016PR China Molecular Oncology Laboratory Department of Orthopaedic Surgery and Rehabilitation MedicineThe University of Chicago Medical CenterChicagoIL 606037USA Department of Oncology The PLA Rocket Force Characteristic Medical CenterBeijing100088PR China Departments of Burn&Plastic Surgery West China Hospital of Sichuan UniversityChengduSichuan Province610041PR China Department of Orthopaedic Surgery The First Affiliated Hospital of Guangzhou University of Chinese MedicineGuangzhouGuangdong Province510405PR China Departments of Orthopaedic Surgery The Affiliated Zhongnan Hospital of Wuhan UniversityWuhanHubei Province430071PR China Department of Orthopaedic Surgery The Affiliated Hospital of Qingdao UniversityQingdaoShandong Province266000PR China Department of Clinical Laboratory Medicine Chongqing General Hospital Affiliated with the University of Chinese Academy of SciencesChongqing400013PR China Ministry of Education Key Laboratory of Diagnostic Medicine and the School of Laboratory Diagnostic MedicineChongqing Medical UniversityChongqing400016PR China Department of Orthopaedics Union Hospital of Tongji Medical CollegeHuazhong University of Science and TechnologyWuhanHubei Province430022PR China Department of Spine Surgery Second Xiangya HospitalCentral South UniversityChangshaHunan Province410008PR China Department of Oncology and Southwest Cancer Center Southwest HospitalArmy Medical UniversityChongqing400038PR China

Intestinal cancers are developed from intestinal epithelial stem cells(ISCs)in intestinal crypts through a multi-step process involved in genetic mutations of oncogenes and tumor suppressor *** play a key role in maintaining the homeostasis of gut *** 2009,Sato et al established a three-dimensional culture system,which mimicked the niche microenvironment by employing the niche factors,and successfully grew crypt ISCs into organoids or Mini-guts in *** then,the intestinal organoid technology has been used to delineate cellular signaling in ISC ***,the cultured organoids consist of heterogeneous cell populations,and it was technically challenging to introduce genomic changes into three-dimensional ***,there was a technical necessity to develop a twodimensional ISC culture system for effective genomic *** this study,we established a conditionally immortalized mouse intestinal crypt(ciMIC)cell line by using a piggyBac transposon-based SV40 T antigen expression *** showed that the ciMICs maintained long-term proliferative activity under two-dimensional niche factor-containing culture condition,retained the biological characteristics of intestinal epithelial stem cells,and could form intestinal organoids in three-dimensional *** in vivo cell implantation tests indicated that the ciMICs were non-tumorigenic,the ciMICs overexpressing oncogenic b-catenin and/or KRAS exhibited high proliferative activity and developed intestinal adenoma-like pathological features in ***,these findings strongly suggested that the engineered ciMICs should be used as a valuable tool cell line to dissect the genetic and/or epigenetic underpinnings of intestinal tumorigenesis.

关键词： Cancer modeling Conditional immortalization Mini-gut organoids Mouse intestinal crypt(MIC)cells Tumorigenesis

急性髓系白血病患者早期血清CCN1蛋白检测及临床价值

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重庆医科大学学报 2022年第2期47卷 197-200页

作者：雷胡芯牛长春杨程廖璞西南医科大学临床医学院检验科泸州646000 陆军军医大学新桥医院血液科重庆400042

目的:研究CCN1蛋白在急性髓系白血病(acute myeloid leukemia,AML)患者早期血清中的表达水平及其与临床特征的相关性,探讨CCN1在早期急性髓系白血病中的诊断价值。方法:收集96例急性髓系白血病早期患者和159名同年龄段、性别匹配的健康... 详细信息

目的:研究CCN1蛋白在急性髓系白血病(acute myeloid leukemia,AML)患者早期血清中的表达水平及其与临床特征的相关性,探讨CCN1在早期急性髓系白血病中的诊断价值。方法:收集96例急性髓系白血病早期患者和159名同年龄段、性别匹配的健康对照者血清样本,采用ELISA检测血清中的CCN1蛋白水平,通过ROC曲线评估其作为诊断标志物的价值;比较CCN1表达水平与临床特征的相关性;分析不同AML分型分组中的血清CCN1表达是否具有差异。结果:AML早期患者血清中的CCN1表达水平明显低于健康对照组(P=0.000),ROC曲线下面积为0.823,最佳cut-off值是101.22 pg/mL,灵敏度为0.823,特异度为0.667。CCN1表达水平与临床特征无相关性。AML不同分型与对照组均有统计学差异(P=0.000),但AML各型之间的CCN1差异无统计学意义(P>0.05)。结论:CCN1蛋白在急性髓系白血病早期患者和正常人血清中表达差异显著,因此CCN1在急性髓系白血病早期诊断中可能有诊断价值。

关键词： CCN1 急性髓系白血病 ROC曲线

Corrigendum to ‘Modeling colorectal tumorigenesis using the organoids derived from conditionally immortalized mouse intestinal crypt cells (ciMICs)’ [Genes Dis 8 (2021) 814-826]

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Genes & Diseases 2023年第2期10卷 618-619页

作者： Xiaoxing Wu Zhaoxia Li Hongyu Zhang Fang He Min Qiao Huaxiu Luo Jing Zhang Meng Zhang Yukun Mao William Wagstaff Yongtao Zhang changchun niu Xia Zhao Hao Wang Linjuan Huang Deyao Shi Qing Liu Na Ni Kai Fu Rex C. Haydon Russell R. Reid Hue H. Luu Tong-Chuan He Ziwei Wang Houjie Liang Bing-Qiang Zhang Ning Wang Departments of Gastrointestinal Surgery Medicine/GastroenterologyAnd Obstetrics and GynecologyThe First Affiliated Hospital of Chongqing Medical UniversityChongqing 400016China Molecular Oncology Laboratory Department of Orthopaedic Surgery and Rehabilitation MedicineThe University of Chicago Medical CenterChicagoIL 606037USA Department of Oncology The PLA Rocket Force Characteristic Medical CenterBeijing 100088China Departments of Burn & Plastic Surgery West China Hospital of Sichuan UniversityChengduSichuan 610041China Department of Orthopaedic Surgery The First Affiliated Hospital of Guangzhou University of Chinese MedicineGuangzhouGuangdong 510405China Departments of Orthopaedic Surgery The Affiliated Zhongnan Hospital of Wuhan UniversityWuhanHubei 430071China Department of Orthopaedic Surgery The Affiliated Hospital of Qingdao UniversityQingdaoShandong 266000China Department of Clinical Laboratory Medicine Chongqing General Hospital Affiliated with the University of Chinese Academy of SciencesChongqing 400013China Ministry of Education Key Laboratory of Diagnostic Medicine And the School of Laboratory Diagnostic MedicineChongqing Medical UniversityChongqing 400016China Department of Orthopaedics Union Hospital of Tongji Medical CollegeHuazhong University of Science and TechnologyWuhanHubei 430022China Department of Spine Surgery Second Xiangya HospitalCentral South UniversityChangshaHunan 410008China Department of Oncology and Southwest Cancer Center Southwest HospitalArmy Medical UniversityChongqing 400038China

The authors regret that an image assembly(copy/paste)error in Figure 3D,in which the image for the organoid of"Primary MiCs"group was erroneously duplicated with an image of primary MICs that was previously *** corrected figure is shown *** shown in the corrected Figure 3D,this error does not adversely impact the conclusion of the original *** authors would like to apologise forany inconvenience caused.

关键词： Figure colorectal Cor

Highly expressed BMP9/GDF2 in postnatal mouse liver and lungs may account for its pleiotropic effects on stem cell differentiation,angiogenesis,tumor growth and metabolism

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Genes & Diseases 2020年第2期7卷 235-244页

作者： Wei Liu Zhongliang Deng Zongyue Zeng Jiaming Fan Yixiao Feng Xi Wang Daigui Cao Bo Zhang Lijuan Yang Bin Liu Mikhail Pakvasa William Wagstaff Xiaoxing Wu Huaxiu Luo Jing Zhang Meng Zhang Fang He Yukun Mao Huiming Ding Yongtao Zhang changchun niu Rex C.Haydon Hue H.Luu Jennifer Moriatis Wolf Michael J.Lee Wei Huang Tong-Chuan He Yulong Zou Departments of Orthopedic Surgery Breast SurgeryGastrointestinal SurgeryObstetrics and Gynecologyand NephrologyThe First Affiliated Hospital of Chongqing Medical UniversityChongqing 400016China Molecular Oncology Laboratory Department of Orthopaedic Surgery and Rehabilitation MedicineThe University of Chicago Medical CenterChicagoIL 60637USA Department of Orthopaedic Surgery The Second Affiliated Hospital of Chongqing Medical UniversityChongqing 400010China Ministry of Education Key Laboratory of Diagnostic Medicine The School of Laboratory MedicineChongqing Medical UniversityChongqing 400016China Departments of Orthopaedic Surgery and Laboratory Diagnostic Medicine Chongqing General HospitalChongqing 400021China Key Laboratory of Orthopaedic Surgery of Gansu Province The Departments of Orthopaedic Surgery and Obstetrics and GynecologyThe First and Second Hospitals of Lanzhou UniversityLanzhou730030China School of Life Sciences Southwest UniversityChongqing 400715China Department of Burn and Plastic Surgery West China Hospital of Sichuan UniversityChengdu 610041China Department of Orthopaedic Surgery The First Affiliated Hospital of Guangzhou University of Chinese MedicineGuangzhou 510405China Department of Orthopaedic Surgery The Affiliated Zhongnan Hospital of Wuhan UniversityWuhan 430072China Department of Orthopaedic Surgery BenQ Medical Center Affiliated with Nanjing Medical UniversityNanjing 210000China Department of Orthopaedic Surgery The Affiliated Hospital of Qingdao UniversityQingdao 266061China

Bone morphogenetic protein 9(BMP9)(or GDF2)was originally identified from fetal mouse liver cDNA *** evidence indicates BMP9 exerts diverse and pleiotropic functions during postnatal development and in maintaining tissue ***,the expression landscape of BMP9 signaling during development and/or in adult tissues remains to be ***,we conducted a comprehensive analysis of the expression landscape of BMP9 and its signaling mediators in postnatal *** analyzing mouse ENCODE transcriptome datasets we found Bmp9 was highly expressed in the liver and detectable in embryonic brain,adult lungs and adult *** next conducted a comprehensive qPCR analysis of RNAs isolated from major mouse tissues/organs at various *** found that Bmp9 was highly expressed in the liver and lung tissues of young adult mice,but decreased in older ***,Bmp9 was only expressed at low to modest levels in developing ***9-associated TGFβ/BMPR type I receptor Alk1 was highly expressed in the adult ***,the feedback inhibitor Smads Smad6 and Smad7 were widely expressed in mouse postnatal ***,the BMP signaling antagonist noggin was highly expressed in fat and heart in the older age groups,as well as in kidney,liver and lungs in a biphasic ***,our findings indicate that the circulating BMP9 produced in liver and lungs may account for its pleiotropic effects on postnatal tissues/organs although possible roles of BMP9 signaling in liver and lungs remain to be fully understood.

关键词： BMP9/GDF2 Bone morphogenetic proteins(BMPs) Hepatic metabolism Mesenchymal stem cells Neurogenesis Osteogenic differentiation Pulmonary arterial hypertension Tumorigenesis

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蛋白质组学在结核病研究中的应用

重庆医学 2023年第13期52卷 2035-2039页

作者：徐越红(综述) 牛长春(审校) 重庆医科大学 400016 重庆市人民医院检验科 401121

目前,结核病诊断方法的特异度和灵敏度不能满足临床诊疗需求,亟须新的方法策略。与传统检测方法相比,蛋白质组学检测具有高通量、高灵敏度和高特异性的优点,在结核病诊疗中具有较好应用前景。该文综述了蛋白质组学的基本概念、发展历史... 详细信息

目前,结核病诊断方法的特异度和灵敏度不能满足临床诊疗需求,亟须新的方法策略。与传统检测方法相比,蛋白质组学检测具有高通量、高灵敏度和高特异性的优点,在结核病诊疗中具有较好应用前景。该文综述了蛋白质组学的基本概念、发展历史及常用蛋白质组数据库,概述了蛋白质组学在结核病诊断、预测结核病感染状态变化、监测结核病治疗效果,以及耐多药结核病生物标志物筛选中的应用,并且指出了蛋白质组学作为结核病筛选生物标志物存在的一些问题,如成本较高,操作流程不一致对检验结果产生影响,高通量检测结果数据后续分析困难等。

关键词：蛋白质组学活动性结核病潜伏性结核感染生物标志物

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“积极保护”理念与济南老城保护规划

规划师 2012年第8期28卷 84-87页

作者：王新文牛长春张中堃王洪梅济南市济南市规划局城乡规划处济南市城乡规划编制研究中心

进入21世纪,济南市城市化进程不断加快,保护与发展的矛盾日益突出。如何处理老城保护与经济发展的关系,如何在保护特色风貌的同时实现老城的现代化,成为急需解决的问题。济南市通过对老城保护存在问题的分析,阐述了基于规划管理角度的"... 详细信息

进入21世纪,济南市城市化进程不断加快,保护与发展的矛盾日益突出。如何处理老城保护与经济发展的关系,如何在保护特色风貌的同时实现老城的现代化,成为急需解决的问题。济南市通过对老城保护存在问题的分析,阐述了基于规划管理角度的"积极保护"理念内容,即注重保护的科学、统筹、整体、重点、有机、特色六个方面,并编制了《济南商埠风貌区规划》等规划,在实践层面进行了探索,提出了相应的实施建议。

关键词：积极保护老城保护济南

Notch signaling:Its essential roles in bone and craniofacial development

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Genes & Diseases 2021年第1期8卷 8-24页

作者： Mikhail Pakvasa Pranav Haravu Michael Boachie-Mensah Alonzo Jones Elam Coalson Junyi Liao Zongyue Zeng Di Wu Kevin Qin Xiaoxing Wu Huaxiu Luo Jing Zhang Meng Zhang Fang He Yukun Mao Yongtao Zhang changchun niu Meng Wu Xia Zhao Hao Wang Linjuan Huang Deyao Shi Qing Liu Na Ni Kai Fu Michael J.Lee Jennifer Moriatis Wolf Aravind Athiviraham Sherwin S.Ho Tong-Chuan He Kelly Hynes Jason Strelzow Mostafa El Dafrawy Russell R.Reid Molecular Oncology Laboratory Department of Orthopaedic Surgery and Rehabilitation MedicineThe University of Chicago Medical CenterChicagoIL 60637USA Pritzker School of Medicine University of ChicagoChicagoIL 60637USA Section of Plastic and Reconstructive Surgery Department of SurgeryThe University of Chicago Medical CenterChicagoIL 60637USA Departments of Orthopaedic Surgery Gastrointestinal SurgeryObstetrics and Gynecologyand NephrologyThe First Affiliated Hospital of Chongqing Medical UniversityChongqing400016PR China Ministry of Education Key Laboratory of Diagnostic Medicine and School of Laboratory and Diagnostic MedicineChongqing Medical UniversityChongqing400016PR China Department of Burn and Plastic Surgery West China Hospital of Sichuan UniversityChengduSichuan610041PR China Department of Orthopaedic Surgery The First Affiliated HospitalGuangzhou University of Chinese MedicineGuangzhouGuangdong510405PR China Departments of Orthopaedic Surgery and Neurosurgery The Affiliated Zhongnan Hospital of Wuhan UniversityWuhanHubei430072PR China Department of Orthopaedic Surgery The Affiliated Hospital of Qingdao UniversityQingdaoShandong266061PR China Department of Laboratory Diagnostic Medicine Chongqing General HospitalChongqing400021PR China Institute of Bone and Joint Research and the Department of Orthopaedic SurgeryThe Second Hospitals of Lanzhou UniversityGansuLanzhou730030PR China Department of Orthopaedics Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhanHubei430072PR China Department of Spine Surgery Second Xiangya HospitalCentral South UniversityChangshaHunan410011PR China

Notch is a cellecell signaling pathway that is involved in a host of activities including development,oncogenesis,skeletal homeostasis,and much *** specifically,recent research has demonstrated the importance of Notch signaling in osteogenic differentiation,bone healing,and in the development of the *** craniofacial skeleton is complex and understanding its development has remained an important focus in *** this review we briefly summarize what recent research has revealed about Notch signaling and the current understanding of how the skeleton,skull,and face *** then discuss the crucial role that Notch plays in both craniofacial development and the skeletal system,and what importance it may play in the future.

关键词： Alagille syndrome Bone Craniofacial development Craniosynostosis Notch Oncogenesis Osteogenesis Spondylocostal dysosotosis