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Effects of Serum Concentration, Synchronization Time and Confluence on the Cell-Cycle Synchronization Efficiency of Goat Fibroblasts

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Open Journal of Animal Sciences 2024年第3期14卷 194-203页

作者： Van Khanh Nguyen Huong Thu Thi Vu Au Thi Hoang Yen Kim Thi Pham Giang Thi Thanh Nhan hung Phu Lai Lan Doan Pham Key Laboratory of Animal Cell Biotechnology National Institute of Animal Science Hanoi Vietnam

This study aims to evaluate the effect of serum concentration, synchronization time, and confluence degree on the synchronisation efficiency of goat fibroblast cycle. The results indicated that there was no difference in the percentage of nucleated fibroblasts in the G0/G1 stage between serum concentrations of 0.3% and 0.4% (83.89% and 82.69%, respectively, P > 0.05) as well as between serum concentrations of 0.2% and 0.5% (76.95% and 75.46%, respectively, P > 0.05). The percentage of nucleated fibroblasts in the G0/G1 stage was highest at the concentration of 0.3% and lowest in the control group (83.89% vs. 62.67%, P 0.05). The beneficial effect of high confluence was confirmed by the large percentage of nucleated fibroblasts at the G0/G1 stage. The 60% confluency was significantly lower than the 80% and 100% confluency (73.44%, 86.63%, and 87.17%, respectively, P < 0.05). The results indicate that the goat fibroblast cycle synchronization is the most effective at the serum concentration of 0.3%, 72 hours of synchronization and 100% confluency.

关键词： Goat Fibroblast Cycle Synchronisation Serum Concentration Synchronization Time Confluence

Enhancement of Swimming Endurance by Herbal Supplement M3P

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Chinese Journal of Integrative Medicine 2022年第8期28卷 725-729页

作者： CHU Chien-ming CHI Chih-wen HUANG Chih-hung CHEN yu-jen Graduate Institute of Biochemical and Biomedical Engineering National Taipei University of TechnologyTaipeiTaiwan10608China BioMedical Development Center MacKay Memorial HospitalNew Taipei CityTaiwan25160China Department of Medical Research MacKay Memorial HospitalNew Taipei CityTaiwan25160China Department of Radiation Oncology MacKay Memorial HospitalTaipeiTaiwan10449China

Objective:To investigate the effect of M3P(containing Deer antler,Cordyceps sinensis,Rhodiola rosea,and Panax ginseng);an herbal remedy with the function of tonifying Kidney(Shen)and invigorating Spleen(Pi),replenishing qi and nourishing blood;on fatigue alleviation,endurance capacity and ***:Swimming with weight-loading of 24 male ICR mice was used to evaluate the endurance capacity,and fatiguerelated plasma biomarkers were *** were randomly assigned to control or M3P treatment groups with 6 mice for each group and were orally administered with M3P everyday for 8 weeks at doses 0,10,33 or 100 mg/*** time to exhaustion was measured in a specialized water *** and kidney functions,body weight,and hematological profile were determined to evaluate the safety and toxicity after long-term M3P ***:M3P supplementation 100 mg/kg significantly increased swimming endurance time up to approximate 2.4 folds of controls(Pchanged after M3P treatment(P>0.05).The liver and kidney functions muscle damage biomarker creatine,body weight,and hemograms were not altered in M3P supplementation(P>0.05).Conclusion:M3P supplementation may improve swimming endurance accompanied by increasing hepatic glycogen content and serum cortisol level without major toxicity.

关键词： swimming endurance fatigue tonifying Kidndy(Shen)and invigorating Spleen(Pi) replenishing qi and nourishing blood M3P mice Chinese medicine

A high-fidelity design methodology using LES-based simulation and POD-based emulation:A case study of swirl injectors

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Chinese Journal of Aeronautics 2018年第9期31卷 1855-1869页

作者： Xingjian WANG Shiang-Ting YEH yu-hung chang Vigor YANG School of Aerospace Engineering Georgia Institute of Technology

Engineering design is undergoing a paradigm shift from design for performance to design for affordability, operability, and durability, seeking multi-objective optimization. To facilitate this transformation, significantly extended design freedom and knowledge must be available in the early design stages. This paper presents a high-fidelity framework for design and optimization of the liquid swirl injectors that are widely used in aerospace propulsion and power-generation systems. The framework assembles a set of techniques, including Design Of Experiment(DOE), high-fidelity Large Eddy Simulations(LES), machine learning, Proper Orthogonal Decomposition(POD)-based Kriging surrogate modeling(emulation), inverse problem optimization, and uncertainty quantification. LES-based simulations can reveal detailed spatiotemporal evolution of flow structures and flame dynamics in a high-fidelity manner, and identify important injector design parameters according to their effects on propellant mixing, flame stabilization, and thermal *** a given a space of design parameters, DOE determines the number of design points to perform LES-based simulations. POD-based emulations, trained by the LES database, can effectively explore the design space and deduce an optimal group of design parameters in a turn-around time that is reduced by three orders of magnitude. The accuracy of the emulated results is validated, and the uncertainty of prediction is quantified. The proposed design methodology is expected to profoundly extend the knowledge base and reduce the cost for initial design stages.

关键词： Emulation High-fidelity design Kriging Large Eddy Simulation(LES) Proper Orthogonal Decomposition(POD) Swirl injector

An Event Alarm System Based on Single and Group Human Behavior Analysis

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Journal of Electronic Science and Technology 2017年第2期15卷 123-132页

作者： hung-yu Yeh I-Cheng chang yung-Hsin Chen Department of Computer Science&Information Engineering National Dong Hwa UniversityHualien 97401 cal-comp electronics and Communications Company Ltd. Taipei 10571

Due to the increasing demand for security, the development of intelligent surveillance systems has attracted considerable attention in recent years. This study aims to develop a system that is able to identify whether or not the people need help in a public place. Different from previous work, our work considers not only the behaviors of the target person but also the interaction between him and nearby people. In the paper, we propose an event alarm system which can detect the human behaviors and recognize the happening event through integrating the results generated from the single and group behavior analysis. Several new effective features are proposed in the study. Besides, a mechanism capable of extracting one-to-one and multiple-to-one relations is also developed. Experimental results show that the proposed approach can correctly detect human behaviors and provide the alarm messages when emergency events occur.

关键词： Index Terms Event alarm system group behavior analysis human behavior recognition single behavior analysis stooping curve.

Scattering Phase Correction for Semiclassical Quantization Rules in Multi-Dimensional Quantum Systems

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Communications in Theoretical Physics 2010年第2期53卷 250-256页

作者： HUANG Wen-Min MOU Chung-yu chang Cheng-hung Department of Physics National Tsing-Hua University Hsinchu Taiwan physics Division National Center for Theoretical Sciences Hsinchu Taiwan Institute of Physics National Chiao Tung University Hsinchu Taiwan Institute of Mathematical Modeling and Scientific Computing National Chiao Tung University Hsinchu Taiwan

当为几个一个维的潜力的散布阶段能确切被导出时，更少在多维的量系统被知道。这个工作提供一个方法扩大一个维的阶段知识到多维的量子化规则。扩展在 Bogomolny 在井由不同高度的步潜力围住的二量使用的转移操作符方法的例子被说明。... 详细信息

当为几个一个维的潜力的散布阶段能确切被导出时，更少在多维的量系统被知道。这个工作提供一个方法扩大一个维的阶段知识到多维的量子化规则。扩展在 Bogomolny 在井由不同高度的步潜力围住的二量使用的转移操作符方法的例子被说明。这个概括半古典作品方法精确地决定系统的精力光谱，它显示建议阶段修正的实质的角色。理论上，结果能被扩大到另外的半古典作品方法，例如 Gutzwiller 踪迹公式，动态希腊语的第六个字母功能，和半古典作品 LandauerB 映牯椠 t ??? 胴吗？？

关键词：半经典量子化量子系统相位校正多维散射半经典方法 Zeta函数迹公式

Preparation and characterization of nano-rare earth composite materials:application in selectivity catalytic oxidation of ammonia and its cytotoxicity study

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Journal of Rare Earths 2010年第S1期28卷 362-366页

作者： chang-Mao hung Department of Vehicle Engineering Yung-Ta Institute of Technology and Commerce316 Chung-Shan RoadLinloPingtung 909Taiwan

The manufacture,physical characterization,environmental applications and cytotoxicity properties of nanocomposites consisting of CuO/CeO2 nano-rare earth composite materials prepared using the coprecipitation method at molar ratio of 6:4 with aqueous solutions of copper nitrate and cerium nitrate were *** performance of the selective catalytic oxidation of ammonia to N2(NH3-SCO) over a CuO/CeO2 nano-rare earth composite materials in a tubular fixed-bed reactor(TFBR) at temperatures from 423 to 673 K in the presence of oxygen was *** catalytic redox behavior was determined by cyclic voltammetry(CV).The nanocomposite particles were characterized by TEM,with a tiny particle size around 10 nm with high dispersion ***,cell cytotoxicity and the percentage cell survival were determined by using 3-(4,5-dimethylthiazol-2-yl)-5(3-carboxymethoxyphenol)-2-(4-sulfophenyl)-2H-tetra-zolium(MTS) assay on human lung MRC-5 cell *** results showed that no apparent cytotoxicity was observed when the MRC-5 was exposed to the CuO/CeO2 nanocomposite materials.

关键词： selective catalytic oxidation (SCO) tubular fixed-bed reactor (TFBR) ammonia CuO/CeO2 nanocomposite material cytotoxicity rare earths

Laparoscopic common bile duct exploration to treat choledocholithiasis in situs inversus patients:A technical review

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World Journal of Clinical Cases 2023年第9期11卷 1939-1950页

作者： Bo-Ya Chiu Shu-hung Chuang Shih-chang Chuang Kung-Kai Kuo School of Post-baccalaureate Medicine College of MedicineKaohsiung Medical UniversityKaohsiung 80708Taiwan Division of General and Digestive Surgery Department of SurgeryKaohsiung Medical University HospitalKaohsiung Medical UniversityKaohsiung 80708Taiwan School of Medicine College of MedicineKaohsiung Medical UniversityKaohsiung 80708Taiwan

Situs inversus(SI)is a rare congenital condition characterized by a mirror-image transposition of the major visceral *** the 1990s,more than one hundred SI patients have been reported to have successfully undergone laparoscopic *** these cases,the major problem is to overcome is the left-right condition for right-handed *** common bile duct exploration(LCBDE),an alternative to treat patients with bile duct stones,has shown equivalent efficacy and is less likely to cause pancreatitis than endoscopic retrograde *** updated meta-analyses revealed that a shorter postoperative hospital stay,fewer procedural interventions,cost-effectiveness,a higher stone clearance rate,and fewer perioperative complications are additional advantages of ***,the technique is technically demanding,even for skilled laparoscopic *** LCBDE in patients with difficult situations,such as SI,is more complex than *** herein review published SI patients with choledocholithiasis treated by LCBDE,including our own experience,and this paper focuses on the technical aspects.

关键词： Choledocholithiasis Choledochotomy Laparoscopic common bile duct exploration Single incision Situs inversus Transcystic

Living donor liver transplantation for Barcelona clinic liver cancer (BCLC) intermediate-stage hepatocellular carcinoma

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Hepatobiliary Surgery and Nutrition 2023年第2期12卷 169-182页

作者： Ming Chao Tsai Chee-Chien Yong Chih-Che Lin Wei-Chen Lee Chih-Chi Wang Chao-hung hung I-Hsuan Chen yu-Fan Cheng chang-Chun Hsiao Tsung-Hui Hu Chao-Long Chen Division of Hepato-Gastroenterology Department of Internal MedicineKaohsiung Chang Gung Memorial Hospital and Chang Gung University College of MedicineKaohsiung School of Medicine College of MedicineNational Sun Yat-sen UniversityKaohsiung Liver Transplantation Center and Department of Surgery Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of MedicineKaohsiung Division of Liver and Transplantation Surgery Chang Gung Memorial Hospital and Chang Gung University College of MedicineTaoyuan Liver Transplantation Center and Department of Diagnostic Radiology Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of MedicineKaohsiung Graduate Institute of Clinical Medical Sciences College of MedicineChang Gung UniversityDivision of Pulmonary and Critical Care MedicineKaohsiung Chang Gung Memorial HospitalKaohsiung

Background:Barcelona clinic liver cancer(BCLC)stage B(intermediate stage)hepatocellular carcinoma(HCC)is highly heterogeneous;thus,identifying the most effective treatment for individual patients represents a significant clinical ***,transarterial chemoembolization(TACE)is the only recommended treatment ***,we aimed to investigate the patient characteristics and outcomes of living donor liver transplantation(LDLT)for BCLC stage B ***:A total of 516 patients with BCLC stage B HCC who underwent LDLT(n=104)or did not undergo LDLT(non-LDLT;n=412)between 2004 to 2018 were analyzed by propensity score matching(PSM;1:4)*** influencing overall survival(OS)and recurrence were analyzed using Cox’s proportional hazards ***:Patients treated with LDLT achieved better OS than the non-LDLT group,including liver-and non-liver related survival(all P60 years(P=0.006),a neutrophil-lymphocyte ratio(NLR)>4(P=0.016)and>3 locoregional therapies(LRT)before LDLT(P60 years(P3 LRT before LDLT(P=0.001)were independent risk factors for *** a combination of age,NLR,and LRT before liver transplantation(LT),the patients can be divided into low-risk(none of risk),intermediate-risk(one of risk),and high risk(more than two of risk)*** were significant differences in the cumulative HCC recurrence(P<0.001)and mortality(P<0.001)rates among the three ***:LDLT may represent a valuable therapeutic option for selected patients with BCLC stage B HCC.

关键词： Living donor liver transplantation(LDLT) Barcelona clinic liver cancer stage B(BCLC stage B) intermediate stage hepatocellular carcinoma(HCC)

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论中国陶瓷八宝纹样的艺术特色与现代运用

佛山陶瓷 2024年第5期34卷 115-116页

作者：黄培华山西工程科技职业大学晋中030619

带有八宝纹样的中国陶瓷是传统文化中富有特色的工艺品,八宝纹样随佛教一同传入中原,在青花、斗彩、粉彩等陶瓷类型上虽有不同的表现,但都彰显着相似的形态特征,即重复与完整、均衡与对称、节奏和韵律;以及相似的审美特征,即形式美、文... 详细信息

带有八宝纹样的中国陶瓷是传统文化中富有特色的工艺品,八宝纹样随佛教一同传入中原,在青花、斗彩、粉彩等陶瓷类型上虽有不同的表现,但都彰显着相似的形态特征,即重复与完整、均衡与对称、节奏和韵律;以及相似的审美特征,即形式美、文化美、象征美。到了现代,八宝纹样中国陶瓷不仅在构图和造型上实现了创新,更广泛地被应用于现代平面设计,实现了中国陶瓷八宝纹样的活态传承。

关键词：中国陶瓷八宝纹样艺术特色现代运用