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changes in Root Hydraulic Conductivity During Wheat Evolution

Journal of Integrative Plant Biology 2005年第3期47卷 302-310页

作者： chang-xingZHAO xi-PingDENG LunSHAN ErnstSTEUDLE Sui-QiZHANG QingYE StateKeyLaboratoryofSoilErosionandDrylandFarmingonLoessPlateau InstituteofSoilandWaterConservationtheChineseAcademyofSciencesandMinistryofWaterResourcesYangling712100China LehrstuhlPflanzenoekologie UniversitatBayreuthBayreuthD-95440Germany

Abstract: A better understanding of the mechanisms of water uptake by plant roots should be vital for improving drought resistance and water use efficiency (WUE). In the present study, we have demonstrated correlations between root system hydraulic conductivity and root characteristics during evolution using six wheat evolution genotypes (solution culture) with different ploidy chromosome sets (Triticum boeoticum Bioss., T. monococcum L.: 2n=2x=14; T. dicoccides Koern., T. dicoccon (Schrank) Schuebl.: 2n = 4x = 28; T. vulgare Vill., T. aestivum L. cv. xiaoyan No. 6: 2n = 6x = 42). The experimental results showed that significant correlations were found between root system hydraulic conductivity and root characteristics of the materials with the increase in ploidy chromosomes (2x→6x) during wheat evolution. Hydraulic conductivity of the wheat root system at the whole-plant level was increased with chromosome ploidy during evolution, which was positively correlated with hydraulic conductivity of single roots, whole plant biomass, root average diameter, and root growth (length, area), whereas the root/shoot ratio had an inverse correlation with the hydraulic conductivity of root system with increasing chromosome ploidy during wheat evolution. Therefore, it is concluded that that the water uptake ability of wheat roots was strengthened from wild to modern cultivated species during evolution, which will provide scientific evidence for genetic breeding to improve the WUE of wheat by genetic engineering.

关键词： chromosome ploidy drought resistance hydraulic conductivity root system water uptake water use efficiency wheat evolution

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NONLINEAR DYNAMICAL ANALYSIS OF BIFURCATION AND CONFLUENCE OF THE PACIFIC WESTERN BOUNDARY CURRENTS

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Journal of Hydrodynamics 2012年第3期24卷 383-390页

作者： NI Guo-xi JIANG Song JU Qiang-chang KONG Ling-hai LCP Institute of Applied Physics and Computational Mathematics Beijing 100088 China

In this paper, we analyze the bifurcation and the confluence of the Pacific western boundary currents by an analytical approach. Applying the conservation law, the geostrophic balance relation and the Bernoulli integral to a reduced gravity model, we get a quantitative relation for the outflow and the inflow, and establish the related formulae for the width and the veering angle of offshore currents under the inflow condition. Furthermore, a comparison between the volume transport based on the observation data and the analytical value for the Pacific western boundary currents is presented, which validates the theoretical analysis.

关键词： Pacific western boundary currents bifurcation confluence volume transport reduced gravity model

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Genetic Evolution Analysis on Wild Isolates of Citrus Tristeza Virus Originated in China Based on Coat Protein Genes Sequences

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Agricultural Sciences in China 2010年第11期9卷 1623-1629页

作者： YI Long zhou chang-yong zhou Yan LI Zhong-an Citrus Research Institute Southwest University~Citrus Research Institute Chinese Academy of Agricultural Sciences Chongqing 400712 P.R. China Jiangxi Navel Orange Engineering and Technology Research Center Gannan Normal University Ganzhou 341000 P.R.China

The coat protein （CP） genes were cloned and sequenced from viral particles of 11 isolates of citrus tristeza virus （CTV） collected from wild citrus plants in China and 4 Chinese isolates from cultivated sweet orange and pummelo varieties, respectively. By analyzing and comparing the nucleotide and amino acid sequences of CP genes, the 11 wild CTV isolates were found over 92% identical with 4 Chinese CTV isolates and 21 exotic CTV isolates from cultivated citrus. From 91 to 100% of the CTV CP gene sequences in wild type citrus plants were generally well conserved. Genetic evolution analysis indicated that the GC% of the CP gene was less than AT%, and more transition were found in the CP genes than transversion with the transition/transversion ratio ranging from 6.3 to 7.0 among species. The substitution frequency was the highest at the third codon, followed by the first and second codon. The ratio of non-synonymous mutations （du） to synonymous mutations （ds） was far lower than 1, suggesting that the CP gene might have experienced purifying selection in the evolution. Phylogenetic analysis revealed that the 11 CTV isolates in Chinese wild type citrus belonged to different phylogenetic clusters, and shared higher homology and closer relationships with other cultivated citrus CTV isolates from different countries, which indicated complicated genetic relationships among the CTV isolates. In addition, CTV isolates with similar biological characteristics usually located into the same clusters. Therefore, the conclusion was drawn that pathogenicity was critical to evolution and origin of CTV.

关键词： wild type citrus citrus tristeza virus sequence comparison phylogenetic evolution

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Matrine promotes colorectal cancer apoptosis by downregulating shank-associated RH domain interactor expression

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World Journal of Gastrointestinal Oncology 2024年第12期16卷 4700-4715页

作者： Yuan-Chen zhou Qian-Qian Wang Ge-Yu-Jia zhou Teng-Fei Yin Dong-Yan Zhao xi-Zhen Sun chang Tan Lei zhou Shu-Kun Yao Graduate School Peking University China-Japan Friendship School of Clinical MedicineBeijing 100029China Department of Gastroenterology China-Japan Friendship Hospital(Institute of Clinical Medical Sciences)Beijing 100029China Department of Gastroenterology Qilu HospitalShandong UniversityJinan 250012Shandong ProvinceChina Department of Gastroenterology The First Affiliated Hospital of Soochow UniversitySuzhou 215006Jiangsu ProvinceChina Department of Gastroenterology Beijing Jishuitan HospitalBeijing 100035China Department of General Surgery China-Japan Friendship HospitalBeijing 100029China Department of Gastroenterology China-Japan Friendship HospitalBeijing 100029China

BACKGROUND The 5-year survival rate of patients with colorectal cancer(CRC)in China is only 56.9%,highlighting the need for new therapeutic *** studies have shown that matrine exhibits antitumor effects by inducing ***,the mechanism by which matrine regulates antiapoptotic proteins in CRC remains *** To identify apoptotic proteins from proteomics and investigate the role of matrine in impeding CRC apoptosis by regulating these *** Tumor and adjacent normal tissues were collected from 52 patients with CRC who underwent surgery between January and December ***-independent acquisition quantitative proteomic analysis was performed to identify differentially expressed apoptotic *** selected apoptotic proteins were identified through their association with tumor-node-metastasis(TNM)stage and prognosis,then confirmed by immunohistochemical(IHC)staining in validation *** vitro,the role of matrine or apoptotic proteins on cancer cells were *** Compared to normal tissues,88 anti-apoptotic proteins from proteomic results were *** them,Shankassociated RH domain interactor(SHARPIN)was identified because of its relationship with TNM stage and overall survival in TCGA *** the IHC-confirmed cohort,SHARPIN was highly expressed in CRC tissues and localized in the *** SHARPIN expression was associated with TNM stage,carbohydrate antigen 153 levels,and gross type compared to low *** knockdown promoted apoptosis,significantly upregulated the expression of Bcl-2 associated agonist of cell death,Bcl-2 associated X protein,caspase 3,and caspase 8,and downregulated B-cell lymphoma-2(P<0.05).Importantly,matrine treatment promoted apoptosis and reversed the proliferation,invasion,and migration of CRC cells by repressing *** SHARPIN was identified as an upregulated anti-apoptotic protein in CRC,and matrine exhibited anticancer effects by downregulating i

关键词： Colorectal cancer Proteomics Shank-associated RH domain interactor Matrine Apoptosis

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Analysis of bending and buckling of pre-twisted beams:A bioinspired study

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Acta Mechanica Sinica 2014年第4期30卷 507-515页

作者： Zi-Long Zhao Hong-Ping Zhao Zheng chang xi-Qiao Feng Institute of Biomechanics and Medical Engineering AML Department of Engineering MechanicsTsinghua University Center for Nano and Micro Mechanics Tsinghua University

Twisting chirality is widely observed in artificial and natural materials and structures at different length scales. In this paper, we theoretically investigate the effect of twisting chiral morphology on the mechanical properties of elas- tic beams by using the Timoshenko beam model. Particular attention is paid to the transverse bending and axial buckling of a pre-twisted rectangular beam. The analytical solution is first derived for the deflection of a clamped-free beam under a uniformly or periodically distributed transverse force. The critical buckling condition of the beam subjected to its self- weight and an axial compressive force is further solved. The results show that the twisting morphology can significantly improve the resistance of beams to both transverse bending and axial buckling. This study helps understand some phenomena associated with twisting chirality in nature and provides inspirations for the design of novel devices and structures.

关键词： Twisting chirality. Timoshenko beam. Bending.Euler buckling · Bionics

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The putative tumor suppressor C53 interacts with the human telomerase reverse transcriptase hTERT and regulates telomerase activity

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Chinese Science Bulletin 2014年第19期59卷 2324-2330页

作者： Junzhi zhou Peng xi Qi zhou Deqiang Ding Yusheng Cong Key Laboratory for Cell Proliferation and Regulation Biology of Ministry of Education Institute of Cell Biology College of Life Sciences Beijing Normal University Institute of Aging Research Hangzhou Normal University School of Medicine

Telomerase is a large ribonucleoprotein complex that contains a catalytic telomerase reverse transcriptase(TERT)and an RNA *** activity is tightly controlled by TERT expression,which is regulated at both the transcriptional and post-translational ***,the detailed molecular mechanisms of telomerase regulation and function are not fully *** identify cofactors that contribute to telomerase regulation,we employed a yeast two-hybrid system to screen for hTERT-interacting proteins,using the hTERT T-motif as *** identify C53 as a novel hTERT interaction *** show that C53 interacts with hTERT both in vivo and in vitro.C53 depletion increases telomerase activity,and C53 overexpression inhibits telomerase activity in MCF7 *** addition,the C53 leucine zipper domain(amino acids 301–400)is required for interaction with *** the leucine zipper domain eliminates C53 interaction with hTERT and abrogates its inhibitory effect on telomerase *** together,our results demonstrate that C53 is a novel hTERT-associated protein that negatively regulates telomerase activity.

关键词：人端粒酶逆转录酶端粒酶活性亮氨酸拉链结构域酵母双杂交系统蛋白复合物 hTERT 相互作用交互

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Corrosion Behavior of Selective Laser Melted AISi10Mg Alloy in NaCl Solution and Its Dependence on Heat Treatment

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Acta Metallurgica Sinica(English Letters) 2020年第3期33卷 327-337页

作者： xin-Hui Gu Jun-xi Zhang xiao-Lei Fan Lai-chang Zhang Shanghai Key Laboratory of Material Protection and Advanced Material in Electric Power Shanghai University of Electric PowerShanghai 200090China School of Engineering Edith Cowan University270 Joondalup DriveJoondalupPerthWA 6027Australia

This work investigates the corrosion behavior of AlSilOMg alloy produced by selective laser melting(SLM) and the counterparts heat-treated at 450-550℃in 3.5 wt% NaCl *** measurements and weight loss tests together with microstructural characterizations were *** SLM-produced alloy displays a microstructure with a continuous network of Si particles,exhibiting superior corrosion *** heat treatment,the Si particles are coarsened and isolated and A1 matrix dissolves into their surrounding area,thereby degrading the corrosion resistance ***,the corrosion resistance of AlSilOMg alloy degrades with increasing the heat treatment temperature.

关键词： Aluminum Selective laser melting XRD Electrochemistry Pitting corrosion

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Ultrasound-targeted cationic microbubble-mediated gene transfection and inhibition of retinal neovascularization

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International Journal of Ophthalmology(English edition) 2022年第6期15卷 876-885页

作者： Ming-xing Wu Yu zhou xi-Yuan zhou Yan Xu Department of Ophthalmology Second Affiliated Hospital of Chongqing Medical UniversityChongqing 400010China

AIM:To investigate whether ultrasound-targeted cationic microbubbles(CMBs)destruction could deliver endostatingreen fluorescent protein(GFP)plasmids efficiently to the human retinal endothelial cells(HRECs)and inhibit retinal neovascularization in ***:CMBs were prepared and the presentation of GFP reporter was confirmed by flow cytometry and laser confocal *** assessing HRECs migration and vascular formation were per formed to evaluate gene therapy’s efficiency in vitro.A mouse model of oxygen-induced retinopathy was employed and the expression of Bcl-xl,Bcl-2,vascular endothelial growth factor(VEGF)and endostatin in the retina of mice were determined by Western blotting and quantitative polymerase chain reaction(q PCR).The expression of endostatin-GFP in the retina was examined by laser confocal microscopy at 5,14,and 28 d after ***:The gene expression of endostatin was the highest in the group of the ***,the inhibition and antiangiogenesis effect of the migration and development of HRECs were improved following treatment with CMBs compared with the other groups in *** vivo,retinal neovascularization was significantly inhibited and the fluorescence intensity of endostatin-GFP in the mouse retina was importantly higher in the group of CMBs than that in other ***:The research illustrates ultrasoundtargeted CMBs destruction possessed distinct effect on the inhibition of the vascular formation and the development of retinal neovascularization both in vitro and in vivo.

关键词： ultrasound cationic microbubbles human retinal vascular endothelial cells gene transfection retinal neovascularization

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From Biological Consciousness to Machine Consciousness:An Approach to Make Smarter Machines

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International Journal of Automation and computing 2013年第6期10卷 498-505页

作者： Xue-Yan Zhang chang-Le zhou Cognitive Science Department Xiamen University Fujian Provincial Key Laboratory of Brain-like Intelligent Systems Xiamen University

Consciousness research has been of great concern to philosophers, psychologists and neuroscientists in recent years. At the same time, consciousness has also attracted more and more interest of artificial intelligence （AI） researchers. In order to make more intelligent machines, many computing models of machine consciousness have been presented. Furthermore, self-consciousness has relevance to the level of intelligent functions. Hence, it is necessary to study self-consciousness in AI. This thesis, starting from biological consciousness, discusses some viewpoints of machine consciousness. Based on the discussions, we present a way to emulate self-consciousness and test this method via simulation experiments. Our results indicate that self-consciousness, which belongs to organisms, can he imitated by machines.

关键词： Artificial intelligence (AI) biological consciousness machine consciousness self-consciousness game problems.

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Tripling Construction for Large Sets of Resolvable Directed Triple Systems

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Acta Mathematica Sinica,English Series 2006年第1期22卷 311-318页

作者： Jun Ling zhou Yan Xun chang Department of Mathematics Beijing Jiaotong University Beijing 100044 P. R. China

In this paper, we first define a doubly transitive resolvable idempotent quasigroup （DTRIQ）, and show that aDTRIQ of order v exists if and only ifv ≡0（mod3） and v ≠ 2（mod4）. Then we use DTRIQ to present a tripling construction for large sets of resolvable directed triple systems, which improves an earlier version of tripling construction by Kang （J. Combin. Designs, 4 （1996）, 301-321）. As an application, we obtain an LRDTS（4·3^n） for any integer n ≥ 1, which provides an infinite family of even orders.

关键词： Doubly transitive resolvable idempotent quasigroup Resolvable directed triple system,Large set Tripling construction

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