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Intramammary expression and therapeutic effect of a human lysozyme-expressing vector for treating bovine mastitis

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2006年第4期7卷 324-330页

作者： SUN Huai-chang XUE fang-ming QIAN Ke fang Hao-xia QIU Hua-lei ZHANG Xin-yu YIN Zhao-hua School of Veterinary Medicine Yangzhou University Yangzhou 225009 China

To develop a gene therapy strategy for treating bovine mastitis, a new mammary-specific vector containing human lysozyme (hLYZ) cDNA and kanamycin resistance gene was constructed for intramammary expression and clinical studies. After one time acupuncture or intracisternal infusion of healthy cows with 400 μg of the p215C3LYZ vector, over 2.0 μg/ml of rhLYZ could be detected by enzymatic assay for about 3 weeks in the milk samples. Western blotting showed that rhLYZ secreted into milk samples from the vector-injected cows had molecular weight similar to that of the natural hLYZ in human colostrums. Twenty days after the primary injection, the quarters were re-injected with the same vector by quarter acupuncture and even higher concentrations of rhLYZ could be detected. Indirect competitive ELISA of milk samples showed that the vector injection did not induce detectable humoral immune response against hLYZ. Clinical studies showed that twice acupuncture of quarters with the p215C3LYZ vector had overt therapeutic effect on clinical and subclinical mastitis previously treated with antibiotics, including disappearance of clinical symptoms and relatively high microbiological cure rates. These data provide a solid rationale for using the vector to develop gene therapy for treating bovine mastitis.

关键词： Human lysozyme cDNA Mammary-specific vector Expression Mastitis Gene therapy

Road effects on vegetation composition in a saline environment

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Journal of Plant Ecology 2012年第2期5卷 206-218页

作者： Sheng-Lan Zeng Ting-Ting Zhang Yu Gao Bo Li chang-ming fang S.Luke Flory Bin Zhao Coastal Ecosystems Research Station of the Yangtze River Estuary Ministry of Education Key Laboratory for Biodiversity Science and Ecological EngineeringInstitute of Biodiversity ScienceFudan University220 Han Dan RoadShanghai 200433P.R.China Department of Biology Indiana University1001 East 3rd StreetBloomingtonIN 47405USA

Aims Road effects from maintenance and traffic have the potential to alter plant communities,but the exact relationships between these effects and changes in plant community composition have not often been studied in diverse *** determine the direction and level of community composition changes in saline environment due to road effects,we conducted a study along roads of different ages and in nearby non-road(***)areas in the Yellow River Delta,***,to potentially elucidate the mechanisms underlying the changes in the richness and composition of plant communities along roads,we evaluated physiochemical changes in soil of roadside and non-road *** Floristic and environmental data were collected along roadside of different ages and nearby non-road *** evaluate plant communities at each site,six 2 m 32 m quadrats were placed at 3-m intervals along roads and six quadrats were arranged randomly in non-road *** determine the difference in plant community composition betweenroadside andnon-road areas,wemeasuredspecies richness and the abundance of each species,examined species turnover and floristic dissimilarity between the two areas and positioned plant species and sites in an abstract multivariate *** community(species richness,percentage of halophytes)and soil physicochemical properties(pH,salinity,moisture content,bulk density,nitrate and ammonium nitrogen concentration)were compared between roadside and non-road areas(young roadside *** non-road areas,old roadside *** non-road areas)by using *** and ordination techniques were used to examine the relationship between vegetation and related environmental variables in both roadside and non-road *** Findings For both the young and old roadside areas,species richness in roadside areas was significantly higher than in non-road areas and high floristic dissimilarity values indicated that roadside and non-roa

关键词： road effect salinization species composition vegetation–environment relationships

STUDY ON THE POLYMER ELECTROLYTES OF(PMA/PEG)n:LiClO_4 SYSTEM

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Chinese Chemical Letters 1991年第9期2卷 713-716页

作者： Yan LIU (Department of Chemistry,Qinghai Medical Colleng,xining,810001) Zeng Liang CAI chang ming fang Yi ZHANG Hai Chun GAO (Qinghai Institute of Salt Lakes,Chinese Academy of Science,Xining,810008)

Lithium ion conducting polymer electrolytes were prepared by blending poly(methyl acrylate)[PMA] with poly(ethylene glycol)[PEG]and coordinating them with LiC10_*** highest conductivity was above 1×10^(-6)S/cm at roo... 详细信息

关键词： PEG Li PMA/PEG)n STUDY ON THE POLYMER ELECTROLYTES OF

基于地理信息系统的土地利用变化制图方法研究——以黄河小浪底水库库周为例

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水土保持学报 2003年第2期17卷 110-113页

作者：李道峰柳长顺刘昌明郝芳华北京师范大学环境科学研究所水环境模拟国家重点实验室北京100875

详细介绍了土地利用变化图绘制的一般过程,认为图例的设计和不同时期土地利用现状图的代数叠加运算是整个编制过程的关键,制作过程中应本着简单实用、形象答意和美观大方的原则进行。实例选取黄河小浪底水利枢纽库周1993年和2000年TM遥... 详细信息

详细介绍了土地利用变化图绘制的一般过程,认为图例的设计和不同时期土地利用现状图的代数叠加运算是整个编制过程的关键,制作过程中应本着简单实用、形象答意和美观大方的原则进行。实例选取黄河小浪底水利枢纽库周1993年和2000年TM遥感影像进行土地利用变化分析,编制出建库前后土地利用变化图,计算出土地覆被变化转移矩阵,并对土地利用变化结果进行分析。

关键词：地理信息系统土地利用变化制图方法代数叠加运算黄河小浪底水库库周转移矩阵变化图例

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广大铁路祥云长大隧道配电方案探究

石家庄铁路职业技术学院学报 2017年第3期16卷 12-15页

作者：袁长明战启芳石家庄铁路职业技术学院河北石家庄050061

长大隧道的配电,供电距离长,电压损失大,影响用电设备的正常工作,采用交流电压补偿器不能有效提高其供电距离,只有采用高压进洞才能减少电压损失和电能损失,获得较好的经济性,本文就施工初期的低压进洞及施工中后期的高压进洞方案进行... 详细信息

长大隧道的配电,供电距离长,电压损失大,影响用电设备的正常工作,采用交流电压补偿器不能有效提高其供电距离,只有采用高压进洞才能减少电压损失和电能损失,获得较好的经济性,本文就施工初期的低压进洞及施工中后期的高压进洞方案进行分析和研究,设计了广大铁路祥云隧道高压配电方案,满足了施工设备的用电需求,降低了运行成本。

关键词：长大隧道配电供电距离电压损失电能损失

Direct Photolithography of WO_x Nanoparticles for High-Resolution Non-Emissive Displays

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NANO-MICRO LETTERS 2025年第1期17卷 1-13页

作者： Gu, chang Yang, Guojian Wang, Wenxuan Shi, Aiyan fang, Wenjuan Qian, Lei Hu, Xiaofei Zhang, Ting Xiang, Chaoyu Zhang, Yu-Mo Chinese Acad Sci Ningbo Inst Mat Technol & Engn Lab Optoelect Informat Technol & Devices Ningbo 315201 Peoples R China Qianwan Inst CNITECH Hangzhou Bay Lab Adv Nanooptoelectron Mat & Device Ningbo 315336 Peoples R China Zhejiang Univ Innovat Ctr Yangtze River Delta Smart Mat Architecture Res Lab Jiaxing 314100 Peoples R China Univ Sci & Technol China Hefei 230026 Peoples R China Jilin Univ Coll Chem State Key Lab Supramol Struct & Mat Changchun 130012 Peoples R China

High-resolution non-emissive displays based on electrochromic tungsten oxides (WOx) are crucial for future near-eye virtual/augmented reality interactions, given their impressive attributes such as high environmental stability, ideal outdoor readability, and low energy consumption. However, the limited intrinsic structure of inorganic materials has presented a significant challenge in achieving precise patterning/pixelation at the micron scale. Here, we successfully developed the direct photolithography for WOx nanoparticles based on in situ photo-induced ligand exchange. This strategy enabled us to achieve ultra-high resolution efficiently (line width m, the best resolution for reported inorganic electrochromic materials). Additionally, the resulting device exhibited impressive electrochromic performance, such as fast response ( 3600 cycles), as well as promising applications in electronic logos, pixelated displays, flexible electronics, etc. The success and advancements presented here are expected to inspire and accelerate research and development (R&D) in high-resolution non-emissive displays and other ultra-fine micro-electronics.

关键词： Electrochromic Direct photolithography WOx nanoparticles In situ photo-induced ligand exchange High-resolution displays

Stress Protein GRP170 Localizes Down-stream of c-Myc and Has Oncogenic Potential

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Stress Protein GRP170 Localizes Down-stream of c-Myc and Has...

Inter-Skeleton Conductive Routes Tuning Multifunctional Conductive Foam for Electromagnetic Interference Shielding, Sensing and Thermal Management

中国细胞生物学学会第八届会员代表大会暨学术大会

作者： chang ming fang and Yong Hua Xu (Laboratory of Molecular and Cellular Oncology, Shanghai Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 320 Yue-yang Road, Shanghai, 200031, P.R. China)

Cells up-regulate the expression of molecular chaperones, such as glucose/oxygen-regulated proteins (GRPs/ORPs) and heat-shock proteins (HSPs), when subjected to environmental stress. GRP170/ORP150 is a novel endoplasmic reticulum-associated protein in the HSP70 family, which was first purified for its capacity to promote cell survival under the condition of hypoxia. We here reported that hamster grp170 was highly expressed in CHO-c-mycER cells when c-MycER was activated by the synthetic steroid, 4-hydroxy-tamoxifen (OHT). Moreover, the expression of its human homologue orp150 increased in some of human hepaitocellular carcinomas in which elevated c-myc expression was frequently detected. Our data indicated put GRP170/ORP150 down-steam of c-Myc signal pathway. The forced expression of grp170 in CHO cells stimulated the anchorage-dependent and anchorage-independent growth, reduced the serum dependency of cell growth in vitro. Serum starvation experiments showed that the over-expression of grp170 rescued the CHO cells frohi serum free induced DNA degradation and apoptosis of cells. Furthermore, the grp170 highly expressed CHO cells showed an increased potential of tumorigenicity in nude mice. The results suggest that grp170/orp150 is a down-stream target gene of oncogene c-Myc playing an active role in oncogenic transformation.

关键词： Differential display c-myc glucose/oxygen-regulated protein (GRP/ORP)

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NANO-MICRO LETTERS 2025年第1期17卷 1-18页

作者： Li, Xufeng Chen, Chunyan Li, Zhenyang Yi, Peng Zou, Haihan Deng, Gao fang, ming He, Junzhe Sun, Xin Yu, Ronghai Shui, Jianglan Pan, Caofeng Liu, Xiaofang Beihang Univ Sch Mat Sci & Engn Beijing 100191 Peoples R China Beihang Univ Inst Atom Mfg Beijing 100191 Peoples R China Tianmushan Lab Xixi Octagon City Hangzhou 310023 Peoples R China Beijing Inst Environm Features Sci & Technol Electromagnet Scattering Lab Beijing 100854 Peoples R China

Unique inter-skeleton conductive films are constructed in polymer *** resistance change of the foam can reach four orders of magnitude under *** foam exhibits strain-adaptive electromagnetic interference shielding performance, anti-interference pressure sensor with high sensitivity over a wide pressure range and compression-regulated Joule heating function. Conductive polymer foam (CPF) with excellent compressibility and variable resistance has promising applications in electromagnetic interference (EMI) shielding and other integrated functions for wearable electronics. However, its insufficient change amplitude of resistance with compressive strain generally leads to a degradation of shielding performance during deformation. Here, an innovative loading strategy of conductive materials on polymer foam is proposed to significantly increase the contact probability and contact area of conductive components under compression. Unique inter-skeleton conductive films are constructed by loading alginate-decorated magnetic liquid metal on the polymethacrylate films hanged between the foam skeleton (denoted as AMLM-PM foam). Traditional point contact between conductive skeletons under compression is upgraded to planar contact between conductive films. Therefore, the resistance change of AMLM-PM reaches four orders of magnitude under compression. Moreover, the inter-skeleton conductive films can improve the mechanical strength of foam, prevent the leakage of liquid metal and increase the scattering area of EM wave. AMLM-PM foam has strain-adaptive EMI shielding performance and shows compression-enhanced shielding effectiveness, solving the problem of traditional CPFs upon compression. The upgrade of resistance response also enables foam to achieve sensitive pressure sensing over a wide pressure range and compression-regulated Joule heating function.

关键词： Inter-skeleton conductive films Conductive polymer foam Liquid metal Electromagnetic interference shielding

A novel dynamic proportional-type iterative learning control design for non-linear systems

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Journal of Control and Decision 2018年第3期5卷 265-276页

作者： ming-fang chang Harbin Institute of Technology Shenzhen Graduate School ShenzhenP.R.China

This paper presents a model-free adaptive iterative learning control(ILC)scheme called a proportional-type ILC scheme for non-linear *** obvious characteristic of the proposed ILC scheme is that we can easily finish the ILC task just utilising the Lipschitz constant of the *** the proposed ILC scheme,the time-vary learning gain can be produced merely by input and output(I/O)***,the convergence conclusion can be expressed by the ranges of the pseudo-partial derivative and the learning *** actual operation,a reasonable and useful convergence condition by a constant is also provided for *** last,the effectiveness of the proposed ILC scheme is shown by simulations.

关键词： terative learning control proportional-type non-linear systems data-driven