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Effects of Cordyceps militaris extract on angiogenesis and tumor growth

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Acta Pharmacologica Sinica 2004年第5期25卷 115-123页

作者： Hwa-seung YOO,Jang-woo SHIN,Jung-hyo CHO,chang-gue son,Yeon-weol LEE,Sang-yong PARK,Chong-kwan CHO Daejeon University Daejeon 301-724 Department of East-West Cancer Center College of Oriental Medicine Daejeon University Daejeon 301-724 Department of East-West Cancer Center College of Oriental Medicine Daejeon University Daejeon 301-724 Department of East-West Cancer Center R&D Lab of Bulrolife Co Ltd Daejeon Korea College of Oriental Medicine Daejeon University Daejeon 301-724 Institute ofTraditional Medicine and Bioscience Daejeon University Daejeon 301-724 Department of East-West Cancer Center College of Oriental Medicine

AIM: To evaluate the effects of Cordyceps militaris extract (CME) on angiogenesis and tumor growth. METHODS:Human umbilical vein endothelial cells (HUVEC), HT1080, and B16-F10 cells were used. DNA fragment, angio-genic related gene expressions (MMPs, bFGF, VEGF, etc), capillary tube formation, wound healing in vitro, tumorgrowth in vivo were measured. RESULTS: CME inhibited growth of HUVECs and HT1080 (P<0.01). CME 100and 200 mg/L reduced MMP-2 gene expression in HT1080 cells by 6.0 % and 22.9 % after 3-h and 14.9 % and32.8 % after 6-h treatment. CME did not affect MMP-9 gene expression in B16-F10 melanoma cells. CME 100 and200 mg/L also reduced bFGF gene expression in HUVECs by 22.2 % and 41.3 %. CME inhibited tube formation ofendothelial cells in vitro and in vivo. CME repressed the growth of B16-F10 melanoma cells in mice compared withcontrol group (P<0.05). CONCLUSION: CME has antiangiogenetic properties.

关键词： Cordyceps militaris angiogenesis human umbilical vein endothelial cells HT1080 cells B16-F10cells matrix metalloproteinases basic fibroblast growth factor

Myelophil, a mixture of Astragali Radix and Salviae Radix extract, moderates toxic side effects of fluorouracil in mice

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World Journal of Gastroenterology 2008年第15期14卷 2323-2328页

作者： Jang Woo Shin Myong Min Lee Jin Young son Nam Heon Lee Chong Kwan Cho Won Kyu Chung Jung Hyo Cho chang gue son East-West Cancer Center Dunsan Oriental Hospital of Daejeon University 1136 Dunsan-dong Seo-gu Daejeon 302122 Korea Department of Radiotherapy Hospital of Konyang University 865 Gasuwon-dong Seo-gu Daejeon 302718 Korea

AIM: To evaluate the efficacy of Myelophil, an extract containing Astragali Radix and Salviae Radix, for reducing complications induced by 5-fluorouracil (5-FU) in a gastrointestinal cancer model. METHODS: We injected 5-FU into mice and then administered Myelophil to examine the ability of the drug to treat the side effects of 5-FU in mice. Peripheral blood counts, histological examinations, and colony-forming assays of bone marrow were conducted, followed by swimming tests and assessment of survival times. RESULTS: Myelophil restored red and white blood cells and platelets in blood, and recovered cell density in bone marrow to levels comparable to those observed within the control group. In addition, Myelophil significantly increased colony-forming unit granulocyte-macrophage (CFU-GM) and CFU-erythroid (CFU-E) compared to the control group. We confirmed that interleukin-3 gene expression was upregulated by Myelophil in spleen cells. Myelophil administration also doubled the survival rate of mice that were severely myelosuppressed as a result of 5-FU injection at a lethal dose of 70%. Finally, the swimming performance of mice significantly improved as a result of Myelophil treatment. CONCLUSION: These results provide experimentalevidence in support of clinical applications of Myelophil to minimize 5-FU-induced myelosuppression and improve general post-chemotherapy health.

关键词： Astragalus membranaceus Salvia miltionrhizae Myelosuppression Immunosuppression Chemotherapy

Soluble components of Hericium erinaceum induce NK cell activation via production of interleukin-12 in mice splenocytes

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Acta Pharmacologica Sinica 2007年第6期28卷 901-907页

作者： Myung-hyun YIM Jang-woo SHIN Jin-young son Se-mi OH Seung-hyun HAN Jung-hyo CHO Chong-kwan CHO Hwa-seung YOO Yeon-weol LEE chang-gue son East-West Cancer Center Dunsan Oriental Hospital of Daejeon UniversityDaejeon 302-122Korea

Aim:To investigate the immunoregulatory functions of water extracts of Hericiumerinaceum （WEHE） focusing on natural killer （NK） cell-based anticancer ***:Mouse splenocytes or purely isolated NK ceils were stimulated with1-100 mg/L WEHE for 24 h followed by co-culture with51Cr-labled Yac-1 cells for4 h,then NK cell-derived cytolytic activity was measured using a *** antibodies against mouse interleukin-12 （IL-12） were addedinto the WEHE-stimulated splenocytes,thereafter,cytotoxicity was measured toexamine the involvement of ***-PCR and ELISA analyses were performed toconfirm the induction of transcription and the translation of IL-12 and interferon-gamma （IFN-gamma） in the WEHE-treated ***:WEHE enhancedthe cytolytic activity of total splenocytes towards Yac-1 ceils in a ***,this activation was not observed when the NK cells isolatedfrom the splenocytes were treated with ***,the treatment withantibodies against IL-12 abolished the effect of WEHE on splenocyte-derivedcytolytic ***-PCR and ELISA analyses showed the induction of IL-12 andIFN-gamma in the WEHE-treated ***:WEHE indirectlyactivates the cytolytic ability of NK cells via the induction of IL-12 in totalsplenocytes,and possibly via other immuno-mediators or cellular components.

关键词： Hericium erinaceum NK cells interleukin-12

Treatment with Modified Bazhen Decoction(八珍汤)for A Patient with Autoimmune Hemolytic Anemia: A Case Report of A Ten-Month Period

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Chinese Journal of Integrative Medicine 2014年第4期20卷 296-299页

作者： Nam-Hun Lee Jung-Hyo Cho chang-gue son Department of Oriental Internal Medicine Cheonan Oriental HospitalOriental Medical CollegeDaejeon University Liver and Immunology Research Center Daejeon Oriental HospitalOriental Medical College

Autoimmune hemolytic anemia（AIHA）is uncommon but prospective fatal condition with an incidence rate of 1-3 per 100,000 persons.;（1,2）]AIHA is characterized by autoantibodies with specificity against red blood cell（RBC）antigens leading to an increased removal of red cells from blood circulation.;（1,3）]AIHA responds well to steroids,but most patients remain steroid-dependent,and many cases require secondline ***,splenectomy can be regarded as the most effective and best-evaluated second-line therapy,but there are still only limited data on longterm efficacy and adverse *** monoclonal anti-CD20 antibody rituximab,another second-line

关键词：八珍汤气血双补剂 AIHA A Case Report of A Ten-Month Period for A Patient with Autoimmune Hemolytic Anemia

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Chunggan extract, a traditional herbal formula, ameliorated alcohol-induced hepatic injury in rat model

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World Journal of Gastroenterology 2014年第42期20卷 15703-15714页

作者： Hyeong-Geug Kim Jung-Min Kim Jong-Min Han Jin-Seok Lee Min-Kyung Choi Dong-Soo Lee Yeon-Hwa Park chang-gue son Liver and immunology research center Daejeon Oriental Hospital of Oriental Medical Collage of Daejeon University NAR center Inc. Daejeon Oriental Hospital of Oriental Medical Collage of Daejeon University Department of Internal Medicine Daejeon St.Mary’s Hospital The Catholic University of Korea

AIM: To evaluate protective effects of Chunggan extract (CGX), a traditional herbal formula, under 4 wk of alcohol consumption-induced liver injury.

关键词： Alcohol abuse Liver injury Traditional Chinese Medicine Fibrogenic cytokines Hepato stellate cell

Toxicological study of the hepatotherapeutic herbal formula,Chunggan extract,in beagle dogs

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World Journal of Gastroenterology 2006年第46期12卷 7497-7502页

作者： Woo-Jin Choi Jang-Woo Shin Jin-Young son Dong-Seok Seo Hark-Soo Park Seung-Hyun Han Ha-lung Sung Jung-Hyo Cho Chong-Kwan Cho Hwa-Seung Yoo Yeon-Weol Lee chang-gue son East-West Cancer Center Dunsan Oriental Hospital of Oriental medical Collage of Daejeon University 1136Dunsan-dong Seo-gu Daejeon 302-122 South Korea Safety Assess-ment Center Korea Testing and Research Institute for Chemical Industry 7-6 Gomak-ri Wolgot-myeon Gimpo-si Kyonggi-doKorea Department of Oromaxillofacial Infection & Immunity and Dental Research Institute School of DentistrySeoul National University 28 Yongon-Dong Chongno-Gu Seoul110-749 South Korea

AIM: To evaluate the pharmaceutical safety of a Chinese herbal formula, Chunggan extract (CGX), traditionally prescribed as a hepatotherapeutic drug via systemic acute and subacute toxicological study. METHODS: Twenty male dogs and 20 female dogs were fed doses 50 times and 4 times greater than the clinically-recommended drug dosages in an acute and a subacute toxicological study, respectively. Adverse effects were examined by comparing the differences between normal and drug-administered groups using clinical signs, necropsies, histopathologic findings, haematology, urinalysis, and biochemical analysis. RESULTS: In the acute study no change in the body weight, diarrhoea, apetite, mortality rate and histopa- thology of major organs was observed in male or female dogs with a single administration of CGX at 5 g/kg. No drug-induced abnormalities at analysis of histopathology, haematology, urinalysis, and biochemistry were found with any dose of this drug. CONCLUSION: CGX is supposed to be very safe when used in a clinical application with a wide therapeutic index.

关键词： Hepatotherapeutics Herbal remedy Chung-gan extract Safety Toxicological study

An herbal formula,CGX,exerts hepatotherapeutic effects on dimethylnitrosamine-induced chronic liver injury model in rats

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World Journal of Gastroenterology 2006年第38期12卷 6142-6148页

作者： Jang-Woo Shin Jin-Young son Se-Mi Oh Seung-Hyun Han Jing-Hua Wang Jung-Hyo Cho Chong-Kwan Cho Hwa-Seung Yoo Yeon-Weol Lee Myong-Min Lee Xiao Ping Hu chang-gue son East-West Cancer Center Dunsan Oriental Hospitalof Orientai medical College of Daejeon University 1136 Dunsan-dong Seo-gu Daejeon 302-122 South Korea Department of Oromaxillofacial Infection and Immunity and Dental Research Institute School of Dentistry Seoul National University 28 Yongon-Dong Chongno-Gu Seoul 110-749 South Korea

AIM： To evaluate the therapeutic effect of Chunggan extract （CGX）, a modified traditional Chinese hepatotherapeutic herbal, on the dimethylnitrosamine （DMN）-induced chronic liver injury model in rats. METHODS： Liver injuries were induced in Wistar rats by injection of DMN （ip, 10 mg/mL per kg） for 3 consecutive days per week for 4 wk. The rats were administered with CGX Coo, 100 or 200 mg/kg per day） or distilled water as a control daily for 4 wk starting from the 15^th d of the DMN treatment. Biochemical parameters （serum albumin, bilirubin, ALP, AST and ALT）, lipid peroxides, hydroxyproline, as well as histological changes in liver tissues were analyzed. In addition, gene expression of TNF-α, TGF-β, TIMP-1, TIMP-2, PDGF-β, and MMP-2, all of which are known to be associated with liver fibrosis, were analyzed using real-time PCR. RESULTS： CGX administration restored the spleen weight to normal alter having been increased by DMN treatment. Biochemical analysis of the serum demonstrated that CGX significantly decreased the serum level of ALP （P 〈 0.05）, ALT （P 〈 0.02）, and AST （P 〈 0.02） that had been elevated by DMN treatment. CGX administration moderately lowered lipid peroxide production and markedly lowered hydroxyproline generation caused by DMN treatment in accordance with histopathological examination. DMN treatment induced a highly upregulated expression of TNF-α, TGF-β,TIMP-1, TIMP-2, PDGF-β, and MMP-2. Of these, the gene expression encoding PDGF-β and MMP-2 was still further enhanced 2 wk after secession of the 4-wk DMN treatment, and was remarkably ameliorated by CGX administration. CONCLUSION： CGX exhibits hepatotherapeutic properties against chronic hepatocellular destruction and consequential liver fibrosis.

关键词： Dimethylnitrosamine Hepatotherapeutic Herbal medicine PDGF-β Chunggan extract

Systematic Review and Meta-Analysis of Acupuncture for Pain Caused by Liver Cancer

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World Journal of Traditional Chinese Medicine 2022年第3期8卷 402-412页

作者： Xiao-Wen Zhang Yun-Jia Gu Huan-Gan Wu Kun-Shan Li Rui Zhong Qin Qi Pin Wu Jun Ji Hui-Rong Liu Yan Huang chang-gue son Lu-Yi Wu Shanghai Research Institute of Acupuncture and Meridian Yueyang Hospital of Integrated Traditional Chinese and Western MedicineShanghai University of Traditional Chinese MedicineShanghaiChina Shanghai Qigong Research Institute Shanghai University of Traditional Chinese MedicineShanghaiChina Key Laboratory of Acupuncture and Immunological Effects Shanghai University of Traditional Chinese MedicineShanghaiChina Shanghai Research Institute of Acupuncture and Meridian Yueyang Hospital of Integrated Traditional Chinese and Western MedicineKey Laboratory of Acupuncture and Immunological EffectsShanghai University of Traditional Chinese MedicineShanghaiChina Liver and Immunology Research Center Daejeon Oriental Hospital of Daejeon UniversityKorea Shanghai Qigong Research Institute Key Laboratory of Acupuncture and Immunological EffectsShanghai University of Traditional Chinese MedicineShanghaiChina

Objective:The objective of this study is to systematically review and analyze the efficacy of acupuncture for pain caused by primary liver cancer(PLC).Materials and Methods:We searched databases,including PubMed,Medline,Embase,Cochrane Library,Web of Science,China National Knowledge Infrastructure,Chinese Science and Technology Periodicals Database(VIP),Wanfang,and SinoMed/Chinese Biomedical Database(CBM),and retrieved randomized controlled trials(RCTs)that used acupuncture as the primary intervention to treat pain caused by *** investigators then screened the articles,extracted and pooled data,and evaluated the risk of bias of the included articles according to the Cochrane ***5.3 was used for the meta-analysis of eligible ***:A total of 145 articles were retrieved;after screening,8 RCTs involving 496 patients were eventually included in this *** results showed that acupuncture effectively improved cancer pain and was superior to Western ***,acupuncture was fast-acting for pain relief,prolonged the relief,and prevented *** adverse reaction rate was also significantly lower than that of Western *** significant difference was observed in Visual Analog Scale score between acupuncture and Western ***:Acupuncture relieves pain caused by liver cancer and can be used as an adjunct and alternative therapy for drug *** existing research evidence is not yet objective or comprehensive,and more rigorous clinical trials are needed to validate the results.

关键词： Acupuncture liver cancer meta-analysis pain systematic review

Induction of murine interleukin-1 beta expression by water-soluble components from Hericium erinaceum

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Acta Pharmacologica Sinica 2006年第8期27卷 1058-1064页

作者： chang-gue son~2 Jang-woo SHIN~2 Jung-hyo CHO~2 Chong-kwan CHO~2 Cheol-heui YUN~3 Seung-hyun HAN~(4,5,6) ~2East-West Cancer Center,Dunsan Oriental Hospital of Daejon University,Daejon,301-724,Republic of Korea ~3School of Agricultural Biotechnology,Seoul National University,Seoul 151-742,Korea ~4Department of Oromaxillofacial Infection and Immunity and Dental Research Institute,School of Dentistry,Seoul National University.Seoul 110-749,Korea ~5Laboratory Sciences Division,International Vaccine Institute.Seoul 151-818.Korea East-West Cancer Center Dunsan Oriental Hospital of Daejon University Daejon Republic of Korea School of Agricultural Biotechnology Seoul National University Seoul Korea Department of Oromaxillofacial Infection and Immunity and Dental Research Institute School of Dentistry Seoul National University Seoul Korea Laboratory Sciences Division International Vaccine Institute Seoul Korea

Aim:To investigate the inductive effect of water extract from Hericium erinaceum(WEHE)on interleukin-1β(IL-1β)***:A murine macrophagecell-line,RAW 264.7 was stimulated with 1 to 10 mg/L WEHE and inductions of IL-1β protein and its steady state mRNA were examined using a bioassay,Westernblotting,and reverse transcription-polymerase chain reaction(RT-PCR)*** inductive effect of WEHE on IL-1β gene expression was further investigatedby a chloramphenicol acetyltransferase(CAT)reporter gene assay using a tran-sient transfection with pIL-1(870 bp)-CAT where the expression of the CAT genewas regulated by a IL-1β *** electrophoretic mobility shift assay(EMSA)was also performed to examine transcription factors,nuclear factor-kappa B(NF-kB),activator protein 1(AP-1),nuclear factor interleukin-6(NF-IL6),and cAMPresponse element(CRE)/activating transcription factor(ATF).Results:WEHEinduced IL-1β production in both its mRNA and protein expression in a dose-dependent *** inductive effect of WEHE on IL-1β gene expression wasdue to the augmentation of the IL- 1β ***,EMSA showedthat WEHE markedly increased the binding activities of NF-kB,and to a lesserextent,those of AP-1 and NF-IL6 to their cognate DNA recognition sites,whereasCRE/ATF binding remained constant,all of which are known to be involved in theregulation of IL-1β gene ***:WEHE induces IL-1β expres-sion in macrophages at a transcriptional level by enhancing the activation oftranscription factors,NF-kB,NF-IL6,and AP-1.

关键词： Hericium erinaceum interleukin-1 lipopolysaccharide