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A covering liquid method to intensify self-preservation effect for safety of methane hydrate storage and transportation

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Petroleum Science 2022年第3期19卷 1411-1419页

作者： Jun chen Yao-song Zeng Xing-yu yu Qing yuan Tao Wang Bin Deng Ke-Le Yan Jian-Hong Jiang li-Ming Tao chang-Zhong chen Hunan Provincial Key Laboratory of Xiangnan Rare-Precious Metals Compounds and Applications College of Chemistry and Environmental ScienceXiangnan UniversityChenzhouHunan Province423000China School of Chemistry and Chemical Engineering Liaocheng UniversityLiaocheng252059China SINOPEC Research Institute of Safety Engineering 339 Songling RoadQingdao266000China

In this work,experiments and comprehensive insights into the proposed covering liquid method to intensify self-preservation effect for methane(CH_(4))storage are *** CH_(4)hydrate decomposition percentage was 17.6%with the pressure of 0.61 MPa after 12 h at 266.0 K without a covering liquid,which can be reduced to 12.4%,13.8%,13.0%,and 8.3%with the pressure of 0.26 MPa,0.33 MPa,0.51 MPa,and 0.37 MPa by covering with tetrahydrofuran(THF),cyclopentane(CP),cyclohexane,and n-tetradecane,*** the temperature for CH_(4)hydrate decompositionwas 274.2 K,covering with THF,CP,cyclohexane,and n-tetradecane failed to inhibit CH_(4)hydrate *** results suggested that the covering liquid may form a new solid layer(a hydrate layer or other solidified layer)around the CH_(4)hydrate,which inhibit CH_(4)transfer below the freezing point of ***,the new solid layer cannot resist the fast transfer of CH_(4)from decomposed CH_(4)hydrate above the freezing point of *** same phenomenon was also observed in a sodium dodecyl sulfonate(SDS)-dry solution CH_(4)hydrate formation ***,the covering method can only intensify the self-preservation effect below the freezing point of water,but cannot generate a self-preservation effect.

关键词： Decomposition Inhibition Methane hydrate Method Self-preservation effect

Phenolic glycosides from the stems of Homalium stenophyllum

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Chinese Journal of natural Medicines 2021年第3期19卷 225-230页

作者： WU Shou-yuan BAI Meng ZHOU Qi NONG Xu-Hua chen guang-Ying ZHAO Si-yu HAN chang-Ri song Xiao-Ping Key Laboratory of Tropical Medicinal Resource Chemistry of Ministry of Education Hainan Normal UniversityHaikou 571158China Key Laboratory of Tropical Medicinal Plant Chemistry of Hainan Province Hainan Normal UniversityHaikou 571158China Key Laboratory of Medicinal and Edible Plants Resources of Hainan Province Hainan Vocational University of Science and TechnologyHaikou 571126China School of Chemistry and Chemical Engineering Lingnan Normal UniversityZhanjiang 524048China

The phytochemical investigation of the stems of Homalium stenophyllum afforded seven new phenolic glycosides(1-5 and 8-9)and two known compounds(6 and 7).Their structures were elucidated by comprehensive analyses of NMR spectroscopic,mass spectrometric data and chemical ***,their anti-inflammatory activities against the NO production in LPS-induced macrophages were evaluated.

关键词： Homalium stenophyllum Phenolic glycosides Chemical hydrolysis Anti-inflammatory activity

Compound extreme inundation risk of coastal wetlands caused by climate change and anthropogenic activities in the Yellow River Delta,China

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Advances in Climate change Research 2024年第1期15卷 134-147页

作者： Xiao-li WANG Ai-Qing FENG Xi-Yong HOU Qing-chen CHAO Bai-yuan song yu-Bin liU Qi-guang WANG He XU yu-Xin ZHANG Dong li li-Jie DONG yu GUO CAS Key Laboratory of Coastal Environmental Processes and Ecological Remediation Yantai Institute of Coastal Zone ResearchChinese Academy of SciencesYantai 264003China Shandong Key Laboratory of Coastal Environmental Processes Yantai 264003China China Meteorological Administration Key Laboratory for Climate Prediction Studies National Climate CentreBeijing 100081China Tongji Urban Planning Design Research Institute Shanghai 200092China School of Geography and Tourism Qilu Normal UniversityJinan 250200China China Meteorological Administration Training Centre China Meteorological AdministrationBeijing 10008China University of Chinese Academy of Sciences Beijing 100049China Marine Science and Technology College Zhejiang Ocean UniversityZhoushan 316022China

The coastal wetlands of the Yellow River Delta(YRD)in China are crucial for their valuable resources,environmental significance,and economic ***,these wetlands are also vulnerable to the dual threats of climate change and human *** substantial attention to the historical shifts in YRD's coastal wetlands,uncertainties remain regarding their future trajectory in the face of compound risks from climate change and anthropogenic *** on a range of remote sensing data sources,this study undertakes a comprehensive investigation into the evolution of YRD's coastal wetlands between 2000 and ***,the potential fate of coastal wetlands is thoroughly analyzed through the Land Use/Cover change(LUCC)simulation using System Dynamic-Future Land Use Simulation(SD-FLUS)model and the extreme water levels projection integrated future sea-level rise,storm surge,and astronomical high tide in 2030,2050,and 2100 under scenarios of SSP1-2.6,SSP2-4.5,and *** revealed that YRD's coastal wetlands underwent a marked reduction,shrinking by 1688.72 km²from 2000 to *** decline was mostly attributed to the substantial expansion in the areas of artificial wetlands(increasing by 823.78 km2),construction land(increasing by 767.71 km²),and shallow water(increasing by 274.58 km²).Looking ahead to 2030-2100,the fate of coastal wetlands appears to diverge based on different *** the SSP1-2.6 scenario,the area of coastal wetland is projected to experience considerable *** contrast,the SSP5-8.5 scenario anticipates a notable decrease in coastal *** to the inundated area suffered from the current extreme water levels,the study projects a decrease of 6.8%-10.6%in submerged coastal wetlands by 2030 and 9.4%-18.2%by 2050 across all *** 2100,these percentages are projected to decrease by 0.4%(SSP2-4.5)and 27.1%(SSP5-8.5),but increase by 35.7%(SSP1-2.6).Results suggest that coastal wetlands in th

关键词： Climate change Anthropogenic activities Compound extreme risk Coastal wetlands Yellow River Delta

Hydrothermal Synthesis, Crystal Structure and Luminescence Property of a 2D Manganese(Ⅱ) Coordination Polymer

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Chinese Journal of Structural Chemistry 2019年第1期38卷 147-154,5页

作者： yu You-Zhu chang song-Yang HAN Xi chen guang-Xin XUAN Ya-Wei WU Xian-li WANG Fang College of Chemistry and Environmental Engineering Anyang Institute of Technology College of Chemistry and Molecular Engineering Zhengzhou University

Solvothermal reactions of MnCl_2·4H_2O with(pyridin-2-yl)methyl 4-(2-(4-((pyridin-2-yl)methoxy)phenyl)diazenyl)benzoate(L1) via an in situ ligand transformation reaction produced a new coordination polymer, {[Mn_(2.5)Cl_3L_2(H_2O)]·H_2O}_n(1, where HL = 4-(2-(4-((pyridin-2-yl)methoxy)phenyl)diazenyl)benzoic acid). The L1 ligand was decomposed in situ to L^- which was coordinated with Mn(Ⅱ) to form compound 1 in the solvothermal synthesis process. Complex 1 was characterized by IR spectroscopy, powder X-ray diffraction and single-crystal X-ray *** 1 possesses a 2D network. In addition, stability and photoluminescent property of 1 were also investigated.

关键词： synthesis crystal structure Mn(Ⅱ)complex photoluminescent property

Hydrothermal Syntheses, Crystal Structures and Luminescence Properties of Cu(Ⅱ) and Cd(Ⅱ) Complexes Assembled by 6-Hydroxypicolinic Acid and 1,10-Phenanthroline

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Chinese Journal of Structural Chemistry 2019年第4期38卷 651-659页

Two new complexes based on 6-hydroxypicolinic acid(H_2picO) and 1,10-phenanthroline(phen), such as [Cu_4(picO)_4(phen)_4]·12 H_2O(1) and {[Cd_4(picO)_4(phen)_4(H_2O)_2]·5H_2O}_n(2), have been synthesized and characterized by IR, elemental analyses, thermogravimetric analyses and X-ray diffraction technique. Single-crystal X-ray diffraction analyses revealed that the two complexes both crystallize in the triclinic system, space group P(16)-. For complex 1, the picO ligands adopt a tridentate coordination mode to link copper(Ⅱ) ions into dimmers. PicO ligands also adopt a tridentate mode in complex 2, which connects the cadmium(Ⅱ) to form a one-dimensional chain. Moreover, luminescent properties of 1 and 2 were also investigated.

关键词： Cu(Ⅱ) and Cd(Ⅱ) complexes 6-hydroxypicolinic acid crystal structures luminescent properties

ARHGAP4 variants are associated with X-linked early-onset temporal lobe epilepsy

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World Journal of Pediatrics 2024年第8期20卷 859-867页

作者： yuan-yuan Hu Wang song Zhi-Gang liu Xing-guang Ye Hong-Wei Zhang Xin li Jun-Xia Luo Peng-yu Wang Jie Wang Xiao-Fei lin Hong-li Zhu Wei-Ping liao Bin li Xu-Qin chen Department of Neurology Key Laboratory of Neurogenetics and Channelopathies of Guangdong Province and the Ministry of Education of ChinaInstitute of Neuroscience and the Second Affiliated Hospital of Guangzhou Medical UniversityChang-gang-dong RoadGuangzhou510260China Epilepsy Center and Neurology Department of Children’s Hospital of Soochow University Suzhou215000JiangsuChina Department of pediatrics Foshan Women and Children HospitalFoshan528000China Children’s Hospital Affiliated to Shandong University Jinan250000ShandongChina Second Hospital of Shandong University Jinan250000China Huai’an Maternity&Child Healthcare Hospital Huai’an223001China Institute of Neuroscience of guangzhou Medical University and Key Laboratory of Neurogenetics and Channelopathies of guangdong Province and the Ministry of Education of China Chang-gang-dong Road 250Guangzhou510260GuangdongChina

The ARHGAP4 gene(OMIM*300,023),also known as Rho GTPase activating protein 4,encodes a protein that has an essential role in the regulation of small ***4 message Rna(mRna)is highly expressed during the developing and adult nervous *** the protein level,ARHGAP4 is exclusively present in certain brain regions,such as the mesial temporal lobe,specifically the stratum lucidum of the CA3 region[1].ARHGAP4 enhances the GTPase activity of Rho proteins,which modulate morphological alterations in neuron development and synaptic plasticity by interacting with the actin cytoskeleton[2].

关键词： epilepsy alterations stratum

Spatial variation and soil nitrogen potential hotspots in a mixed land cover catchment on the Chinese Loess Plateau

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Journal of Mountain Science 2019年第6期16卷 1353-1366页

作者： yu yun-long JIN Zhao liN Henry WANG yun-qiang ZHAO Ya-li CHU guang-chen ZHANG Jing song Yi ZHENG Han State Key Laboratory of Loess and Quaternary Geology Institute of Earth EnvironmentChinese Academy of SciencesXi’an 710061China University of Chinese Academy of Sciences Beijing 100049China CAS Center for Excellence in Quaternary Science and Global change Chinese Academy of ScienceXi’an 710061China Department of Ecosystem Science and Management the Pennsylvania State UniversityUniversity ParkPA 16802USA Shaanxi Key Laboratory of Accelerator Mass Spectrometry and Application Xi’an AMS CenterXi’an 710061China Key Laboratory of Subsurface Hydrology and Ecological Effect in Arid Region of Ministry of Education School of Environmental Science and EngineeringChang’an UniversityXi’an 710054China

Soil nitrogen(N) is critical to ecosystem services and environmental quality. Hotspots of soil N in areas with high soil moisture have been widely studied, however, their spatial distribution and their linkage with soil N variation have seldom been examined at a catchment scale in areas with low soil water content. We investigated the spatial variation of soil N and its hotspots in a mixed land cover catchment on the Chinese Loess Plateau and used multiple statistical methods to evaluate the effects of the critical environmental factors on soil N variation and potential hotspots. The results demonstrated that land cover, soil moisture, elevation, plan curvature and flow accumulation were the dominant factors affecting the spatial variation of soil nitrate(NN), while land cover and slope aspect were the most important factors impacting the spatial distribution of soil ammonium(AN) and total nitrogen(TN). In the studied catchment, the forestland, gully land and grassland were found to be the potential hotspots of soil NN, AN and TN accumulation, respectively. We concluded that land cover and slope aspect could be proxies to determine the potential hotspots of soil N at the catchment scale. Overall, land cover was the most important factor that resulted in the spatial variations of soil N. The findings may help us to better understand the environmental factors affecting soil N hotspots and their spatial variation at the catchment scale in terrestrial ecosystems.

关键词： Soil biogeochemistry Spatial heterogeneity Multivariate statistical analysis Environmental factors Loess Plateau

Heterologous Expression of Rat Testis GABA_A Receptor β3t Splicing Variant in CHO Cells

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Journal of Reproduction and Contraception 2004年第3期15卷 131-138页

作者： Shi-feng li yu-guang chen yuan-chang YAN Yi-ping li Institute of Biochemistry and Cell Biology Shanghai Institute of Biological Sciences Chinese Academy of Sciences Shanghai 200031 China College of life Science Shanghai University Shanghai 200436 China

Objective To characterize a possible retention function of unique sequence in the 5＇end of rat testis GABAA receptor β3t splicing variant Methods Rat testis GABAA receptor β3t splicing variant cDna was cloned and two eukaryotic expression recombinant plasmids of pEGFP-N1 and pEGFP-C1 were constructed respectively by fusing green fluorescent protein to the N or C-terminus of β3t isoform. The recombinant plasmids were transfected into CHO cells by calcium phosphate co-precipitation method Fluorescence microscope and laser confocal microscope were used to analyze localization of β3t in the transfected cells. Cona-Texas-Red was used to label cell ER and the localization of rat testis β3t splicing variant in CHO cells was determined. Results When rat testis β3t splicing variant was expressed in CHO cells, two expression patterns were delineated, the distributions of uniform and mainly discrete intracellular compartments respectively, The chimera product failed to be translocated into the cell surface when expressed in CliO cells; whereas the β3 subunit of rat brain was incorporated into the plasma membrane. Conclusion The inability of β3t to target into the ER may be a consequence of the unique 25 specific amino acid segments in the N terminus.

关键词： GABAA receptor β3t splicing variant heterologous expression CHO cell

Differential stem cell aging kinetics in Hutchinson-Gilford progeria syndrome and Werner syndrome

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Protein & Cell 2018年第4期9卷 333-350页

作者： Zeming Wu Weiqi Zhang Moshi song Wei Wang Gang Wei Wei li Jinghui Lei yu Huang Yanmei Sang Piu Chan chang chen Jing Qu Keiichiro Suzuki Juan Carlos Izpisua Belmonte guang-Hui liu State Key Laboratory of Stem Cell and Reproductive Biology Institute of Zoology Chinese Academy of Sciences Beijing 100101 China national Laboratory of Biomacromolecules CAS Center for Excellence in Biomacromolecules Institute of Biophysics Chinese Academy of Sciences Beijing 100101 China University of Chinese Academy of Sciences Beijing 100049 China national Clinical Research Center for Geriatric Disorders Xuanwu Hospital of Capital Medical University Beijing 100053 China State Key Laboratory of Biomembrane and Membrane Biotechnology Institute of Zoology Chinese Academy of SciencesBeijing 100101 China Key Laboratory of Computational Biology CAS-MPG Partner Institute for Computational Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200031 China Department of Medical genetics School of Basic Medical Sciences Peking University Health Science Center Beijing100191 China Department of Pediatric Endocrinology and Genetic Metabolism Beijing Children's Hospital Capital Medical UniversityNational Center for Children's Health Beijing 100045 China Institute for Advanced Co-Creation Studies Osaka University Osaka 560-8531 Japan Graduate School of Engineering Science Osaka University Osaka 560-8531 Japan Gene Expression Laboratory Salk Institute for Biological Studies La Jolla 92037 USA Key Laboratory of Regenerative Medicine of Minist~ of Education Institute of Aging and Regenerative Medicine JinanUniversity Guangzhou 510632 China

progeria syndrome （HGPS） and Wemer syndrome （WS） are two of the best characterized human progeroid syndromes. HGPS is caused by a point mutation in lamin A （LMna） gene, resulting in the production of a truncated protein product-progerin. WS is caused by mutations in 14/RN gem）, encoding a loss-of-function RecQ Dna helicase. Here, by gene editing we created isogenic human embryonic stem cells （ESCs） with heterozygous （G608G/＋） or homozygous （G608G/G608G） LMna mutation and biallelic WRN knockout, for modeling HGPS and WS pathogenesis, respectively. While ESCs and endothelial cells （ECs） did not present any features of premature senescence, HGPS- and WS-mesenchymal stem cells （MSCs） showed aging-associated phenotypes with different kinetics. WS-MSCs had early-onset mild premature aging phenotypes while HGPS-MSCs exhibited iate-onset acute premature aging characterisitcs. Taken together, our study compares and contrasts the distinct pathologies underpinning the two premature aging disorders, and provides reliable stem-cell based models to identify new therapeutic strategies for pathological and physiological aging.

关键词： WRN lamin HGPS Werner syndrome,stem cell aging