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Integration of the nuclease protection assay with sandwich hybridization (NPA-SH) for sensitive detection of Heterocapsa triquetra

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Acta Oceanologica Sinica 2018年第5期37卷 107-112页

作者： par. Mirye par. So Yun HWANG Jinik JUNG Seung Won 3LEE Juyun chang man LEE Taek-Kyun South Sea Environment Research Depar.ment Korea Institute of Ocean Science and Technology Geoje 53201 Republic of Korea Bioresources Culture Collection Division Nakdonggang National Institute of Biological Resources Sangju 37242 Republic of Korea Research Strategy Team Korea Marine Environment Management Corporation Seou105718 Republic of Korea

Microalgae are photosynthetic microorganisms that function as primary producers in aquatic ecosystems. Some species of microalgae undergo rapid growth and cause harmful blooms in marine ecosystems. Heterocapsa triquetra is one of the most common bloom-forming species in estuarine and coastal waters worldwide. Although this species does not produce toxins, unlike some other Heterocapsa species, the high density of its blooms can cause significant ecological damage. We developed a H. triquetra species-specific nuclease protection assay sandwich hybridization（NPA-SH） probe that targets the large subunit of ribosomal RNA（LSU r RNA）. We tested probe specificity and sensitivity with five other dinoflagellates that also cause red tides. Our assay detected *** at a concentration of 1.5×10^4 cells/m L, more sensitive than required for a red-tide guidance warning by the Korea Ministry of Oceans and Fisheries in 2015（3.0×10^4 cells/m L）. We also used the NPA-SH assay to monitor H. triquetra in the Tongyeong region of the southern sea area of Korea during 2014. This method could detect *** cells within 3 h. Our assay is useful for monitoring H. triquetra under field conditions.

关键词： nuclease protection assay sandwich hybridization Heterocapsa triquetra red tide monitoring

Landscape changes from 1974 to 1995 in the Upper Minjiang River Basin, China

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Pedosphere 2006年第3期16卷 398-405页

作者： HE Xing-Yuan ZHAO Yong-Hua HU Yuan-man chang Yu ZHOU Qi-Xing Institute of Applied Ecology the Chinese Academy of Sciences Shenyang 110016 （China） College of Earth Science ＆ Land Resources management Chang＇an University Xi＇an 710054 （China） Graduate School of the Chinese Academy of Sciences Beijing 100039 （China）

Landscape changes were traced over the 20 years from 1974 to 1995 in the upper Minjiang River basin, one of the most important forest regions in China, based on satellite image interpretation to provide basic data for local decision-making as well as sustainable landscape use and management. Results revealed that landscape from 1974 to 1995 changed at the regional scale as the area of forestland decreased, while cropland, shrubland, economic forest, grassland, and built-up land increased. Landscape changes mainly occurred in forestland, shrubland, grassland, economic forest, and built-up land. Moreover, the changes among forestland, shrubland, and grassland were the largest, influencing the whole characteristics of the changes in the basin. Analysis of the changes between 1974 and 1995 in the study area indicated that landscape heterogeneity and fragmentation increased, whereas landscape connectivity decreased. There were multiple reasons for landscape changes. A principal component analysis (PCA) was used to quantitatively study driving forces of landscape changes. The PCA results showed that economic and population factors were the principal driving forces of landscape changes from 1974 to 1995 in the upper Minjiang River basin, and that PCA was a suitable method for investigating driving forces of landscape changes.

关键词： driving forces landscape change satellite image quantification principal component analysis

Progress of CRISPR/Cas9 technology in breeding of Brassica napus

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Oil Crop Science 2021年第2期6卷 53-57页

作者： Tao chang Mei Guan Bingqian Zhou Zechuan Peng man Xing Xiaodan Wang Chunyun Guan Agricultural College of Hunan Agricultural University China

Gene editing technology provides important technical basics for the research in plant functional genes and crop genetic ***/Cas9-mediated gene editing is an effective experimental tool for crop genome directed editing in recent years,which has been widely used in many crops as rice,wheat and other ***/Cas9 system was expected to be a powerful experimental tool in genetic improvement and molecular design breeding of *** paper,which based on the development history and the latest research of CRISPR/Cas9-mediated gene editing technology in rapeseed,summarized the progress of CRISPR/Cas9 including plant type improvement,yield traits,quality improvement,disease and stress resistance improvement,yellow seed creation and other utilizes at *** application scope,development direction and target analysis method of this technology in rape were *** problems of CRISPR/cas9 system in rapeseed breeding were analyzed and the improvement strategies were ***,views on direction of rapeseed breeding by gene editing were emphasized.

关键词： CRISPR/Cas9 Brassica napus Genome editing

GLP-1R基因多态性与血压钠钾反应性的相关性分析

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西安交通大学学报（医学版） 2024年第2期45卷 212-218页

作者：常鸣珂褚超杜鸣飞贾昊孙月胡桂霖张玺王丹罗文婧严瑜满子悦汪洋牟建军西安交通大学第一附属医院心内科陕西西安710061

目的研究胰高血糖素样肽-1受体(GLP-1R)基因遗传变异与血压钠钾反应性的关系。方法 2004年在陕西眉县共招募了来自124个家系的514名受试者,并建立了“盐敏感性高血压研究队列”。对受试者进行了为期3 d的正常饮食、7 d的低盐饮食、7 d... 详细信息

目的研究胰高血糖素样肽-1受体(GLP-1R)基因遗传变异与血压钠钾反应性的关系。方法 2004年在陕西眉县共招募了来自124个家系的514名受试者,并建立了“盐敏感性高血压研究队列”。对受试者进行了为期3 d的正常饮食、7 d的低盐饮食、7 d的高盐饮食和7 d的高盐补钾饮食干预,并测量不同干预期的血压,并采集外周血标本。此外,采用MassARRAY检测平台对GLP-1R基因的8个单核苷酸多态性(SNP)进行了分型检测。结果 GLP-1R基因SNP rs9462472与低盐期的收缩压、舒张压和平均动脉压反应呈显著相关性。而SNP rs2268637则与高盐期的收缩压反应呈显著相关性。此外,SNP rs2268637还与高盐补钾饮食期的收缩压和平均动脉压反应呈显著相关性。结论 GLP-1R基因的多态性与血压钠钾反应性密切相关,提示其参与调节血压盐敏感性及钾反应性的发生与发展。

关键词： GLP-1R 基因多态性盐敏感性盐钾高血压

CaTiO_(3)的制备及其在光催化领域应用进展

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精细与专用化学品 2024年第8期32卷 45-47页

作者：季俊红常智敏秦国强齐满富河南佰利联新材料有限公司河南焦作454150

概述了固相法、溶胶-凝胶法、共沉淀法、水热/溶剂热合成法等制备CaTiO_(3)的工艺技术,同时详细探讨CaTiO_(3)光催化剂在CO_(2)还原、污染物降解和催化制氢方面的应用,以期为CaTiO_(3)的制备及光催化剂领域的研究提供参考。

关键词： CaTiO_(3) 光催化进展

耦合敏感性分析与两阶段马尔科夫链蒙特卡洛算法的地下水污染溯源辨识

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地球科学与环境学报 2024年第5期46卷 702-710页

作者：李子乐安永凯闫雪嫚长安大学水利与环境学院陕西西安710054 长安大学旱区地下水文与生态效应教育部重点实验室陕西西安710054 西北大学城市与环境学院陕西西安710127

为高精度地开展地下水污染溯源辨识,在对污染源参数进行敏感性分析的基础上,研究应用两阶段马尔科夫链蒙特卡洛(Markov Chain Monte Carlo,MCMC)算法辨识确定污染源参数;同时,探索应用多层感知机(Multi-layer Perceptron,MLP)方法构建... 详细信息

为高精度地开展地下水污染溯源辨识,在对污染源参数进行敏感性分析的基础上,研究应用两阶段马尔科夫链蒙特卡洛(Markov Chain Monte Carlo,MCMC)算法辨识确定污染源参数;同时,探索应用多层感知机(Multi-layer Perceptron,MLP)方法构建地下水污染运移数值模型的代理模型,用以提高地下水污染溯源辨识的效率。为验证上述方法的有效性和可行性,开展了两个数值算例研究。结果表明:采用MLP方法构建的代理模型对地下水污染运移数值模型的逼近精度高,不仅能够有效提升地下水污染溯源辨识效率,还能保持良好的计算精度;所提出的耦合敏感性分析与两阶段MCMC算法能够显著提升低敏感性污染源参数的辨识精度。

关键词：地下水污染运移溯源辨识数值模型两阶段马尔科夫链蒙特卡洛算法敏感性分析多层感知机代理模型

电子垃圾拆解废气中PBDEs特征与污染控制效果

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中国环境科学 2024年第2期44卷 654-662页

作者：付建平谢丹平杨艳艳青宪张漫雯黄锦琼陈晓燕尹文华吴明亮周长风廖海婷生态环境部华南环境科学研究所广东广州510655

本研究选取某电子垃圾集中拆解园区为对象,对采用的加热烤板和火法冶炼等典型拆解工艺产生的废气以及经不同净化处理系统排放废气中PBDEs的排放水平及其组成特征进行了研究,并进一步分析了各净化处理系统对PBDEs的控制效果.结果表明:加... 详细信息

本研究选取某电子垃圾集中拆解园区为对象,对采用的加热烤板和火法冶炼等典型拆解工艺产生的废气以及经不同净化处理系统排放废气中PBDEs的排放水平及其组成特征进行了研究,并进一步分析了各净化处理系统对PBDEs的控制效果.结果表明:加热烤板和火法冶炼拆解过程PBDEs浓度水平差别较大,其顺序为:电热炉二期(68210±12588)ng/m^(3)>滚板炉(10420±1785)ng/m^(3)>电热炉一期(9960±919)ng/m^(3)>火法冶炼(53±14)ng/m^(3).经净化处理系统后废气中PBDEs的排放浓度水平也具有一定差别,其顺序为:2号净化系统(1754±551)ng/m^(3)>1号净化系统(1630±344)ng/m^(3)>3号净化系统(1220±51)ng/m^(3)>4号净化系统(9.5±1.1)ng/m^(3).对不同拆解过程PBDEs组成特征分析发现,采用加热方式进行拆解的电热炉一期、电热炉二期和滚板炉废气PBDEs组成特征相似,以低溴代的BDE-99和BDE-47为主(两种单体质量分数之和均在60%以上),以高温燃烧方式进行拆解的火法冶炼废气则以高溴代的BDE-209为主(质量分数为52%);而不同拆解生产过程产生的PBDEs经4种净化处理系统后的组成特征基本相似,均以低溴代的BDE-28、BDE-47和BDE-99为主(3种单体的质量分数之和均在50%以上).4种废气净化处理系统对PBDEs的总去除率介于82.1%~97.4%,对不同电子垃圾拆解过程产生的PBDEs均具有较好的污染控制效果.

关键词：电子垃圾多溴二苯醚加热烤板火法冶炼污染控制

Compar.son on wear resistance of nanostructured bainitic bearing steel with and without residual cementite

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Journal of Iron and Steel Research International 2022年第2期29卷 339-349页

作者： Yu-man Qin chang-bo Liu Chun-sheng Zhang Xu-biao Wang Xiao-yan Long Yan-guo Li Zhi-nan Yang Fu-cheng Zhang State Key Laboratory of Metastable Materials Science and Technology Yanshan UniversityQinhuangdao066004HebeiChina National Engineering Research Center for Equipment and Technology of Cold Rolled Strip Yanshan UniversityQinhuangdao066004HebeiChina

The sliding wear property of high-carbon nanostructured bainitic bearing steel with the equal initial hardness and different microstructures was investigated,and the reasons for the difference of wear resistance between the cementite-bearing(CB)and cementite-free(CF)specimens were *** results show that CF specimens have lower mass loss and surface roughness and shallower wear depth than CB specimens during wear *** with CB specimen,CF specimen presents superior wear *** is due to two reasons:(1)a lot of retained austenite in CF specimen is easy to produce TRIP effect and be transformed into martensite during wear process,which notably increased the surface hardness of worn specimen;(2)there is a nondestructive oxide layer in the surface of cementite-free worn specimen,which can protect the surface of worn specimen from *** the combined effect of retained austenite and oxide layer,the loss of matrix is ***,CF specimen exhibits high wear *** reveals that the wear mechanism of high-carbon nanostructured bainitic bearing steel with different microstructures can provide a reference for improving the wear resistance in high-carbon nanostructured bainitic bearing steel in future.

关键词： Cementite Nanostructured bainite Bearing steel Microstructure Wear resistance

Effect of Cesarean Section on the Severity of Postpar.um Hemorrhage in Chinese Women： The Shanxi Study

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Current Medical Science 2018年第4期38卷 618-625页

作者： chang XU Qiang FU Hong-bing TAO Xiao-jun LIN man-li WANG Shu-xu XIA Hao-ling XIONG Depar.ment of Health Administration School of Medicine and Health Management Tongji Medical College Huazhong University of Science and Technology Wuhan 430030 China Depar.ment of Epidemiology and Biostatistics College for Public Health and Social Justice Saint Louis University Saint Louis 63104 USA

Postpar.um hemorrhage （PPH） is one of the most adverse obstetric outcomes. Our aim is to detect the risks of multilevel PPH in different cesarean section （CS） groups [including nullipar.us CS with indications, nullipar.us CS without indications, repeat cesarean （RC）, vaginal birth after cesarean （VBAC）, cesarean after vaginal birth （CAVB）]. We conducted a retrospective cohort study, and the data on 127 145 women collected from January 2014 to May 2016 and from 35 tertiary hospitals in Shanxi province, China, were reviewed. Based on the measuring results of PPH, an ordered logistic regression model was used to analyze the adjusted PPH risks for each of the CS groups, and compar.sons were drawn between them. Finally, a total of 99 066 nullipar.us （77.92%） and 28 079 multipar.us （22.08%） women were observed. The number of CS cases was 61 117, and the rate for CS was 48.07%. A total of 10 029 women did not show indications for CS and accounted for 16.41% of the CS par.urient, whereas 9103 women underwent a repeated cesarean, with a CS frequency of 14.89%. The number of VBAC cases was 989, whose rate was 9.88% in prior CS women. The number （proportions） of PPH was 3658 （2.88%） in L1 （PPH volume： ≥900 and 〈1500 mL）, 520 （0.41%） in L2 （PPH volume： ≥1500 and〈2100 mL）, and 201 （0.16%） in L3 （PPH volume： ≥2100 mL）. The Ln （n= 1, 2, 3, etc.） represented the increasing order of PPH severity. In the adjusted results, compar.d with spontaneous vaginal delivery （SVD） as the reference group, in the adjusted result for nullipar.us, there was a decreased PPH risk in CS with indications （OR： 2.32; CI： 2.04-2.62）, which was lower than that of CS without indications （OR： 2.50; CI： 2.01-2.96）. The highest PPH risk in all subgroups （i.e. nullipar.us and multipar.us groups） was observed in the RC （OR： 3.61; CI： 3.16-4.17）, which was nearly twice higher than that of the VBAC （OR： 1.82; CI： 1.33-2.52）. CAVB （OR： 1.03; CI： 0.65-1.62） showed no significant difference with the reference group.

关键词： postpar.um hemorrhage cesarean section par.ty indications