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HNF-4α determines hepatic differentiation of human mesenchymal stem cells from bone marrow

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World Journal of Gastroenterology 2010年第40期16卷 5092-5103页

作者： Mong-Liang Chen Kuan-Der Lee Huei-Chun Huang Yue-Lin Tsai Yi-Chieh Wu Tzer-Min kuo Cheng-Po Hu Chungming chang Division of Molecular and Genomic Medicine National Health Research Institutes Department of Hematology and Oncology Chang Gung Memorial Hospital Institute of Microbiology and Immunology National Yang-Ming University Department of Life Science Tunghai University

AIM: To investigate the differentiation status and key factors to facilitate hepatic differentiation of human bone-marrow-derived mesenchymal stem cells (MSCs). METHODS: Human MSCs derived from bone marrow were induced into hepatocyte-like cells following a previously published protocol. The differentiation status of the hepatocyte-like cells was compared with various human hepatoma cell lines. Overexpression of hepatocyte nuclear factor (HNF)-4α was mediated by adenovirus infection of these hepatocyte-like cells. The expression of interesting genes was then examined by either re-verse transcription-polymerase chain reaction (RT-PCR) or real-time RT-PCR methods. RESULTS: Our results demonstrated that the differentiation status of hepatocyte-like cells induced from human MSCs was relatively similar to poorly differentiated human hepatoma cell lines. Interestingly, the HNF-4 isoform in induced MSCs and poorly differentiated human hepatoma cell lines was identified as HNF4γ instead of HNF-4α. Overexpression of HNF-4α in induced MSCs significantly enhanced the expression level of hepatic-specific genes, liver-enriched transcription factors, and cytochrome P450 (P450) genes. CONCLUSION: Overexpression of HNF-4α improves the hepatic differentiation of human MSCs from bone marrow and is a simple way of providing better cell sources for clinical applications.

关键词： Bone marrow Cytochrome P450 genes Differentiation of hepatocyte Hepatocyte nuclear factor 4 Human mesenchymal stem cells

Routine blood tests to predict liver fibrosis in chronic hepatitis C

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World Journal of Gastroenterology 2012年第8期18卷 746-753页

作者： Yung-Yu Hsieh Shui-Yi Tung Cheng-Shyong Wu kuo- Liang Wei Chien-Heng Shen Te-Sheng chang Kamfai Lee Yi-Hsiung Lin Department of Gastroenterology and Hepatology Chang Gung Memorial Hospital Chiayi 613 Taiwan chang Gung University College of Medicine Taoyuan 333 Taiwan Chiayi School ChangOung Institute of Technology Chiayi 613 Taiwan Department of Pathology Chang Gung MemorialHospital Chiayi 613 Taiwan Department of Health Care Administration Taiwan Shoufu University Tainan 721 Taiwan

AIM: To verify the usefulness of FibroQ for predicting fi brosis in patients with chronic hepatitis C, compared with other noninvasive tests. METHODS: This retrospective cohort study included 237 consecutive patients with chronic hepatitis C who had undergone percutaneous liver biopsy before treatment. FibroQ, aspartate aminotransferase (AST)/alanine aminotransferase ratio (AAR), AST to platelet ratioindex, cirrhosis discriminant score, age-platelet index (API), Pohl score, FIB-4 index, and Lok's model were calculated and compared. RESULTS: FibroQ, FIB-4, AAR, API and Lok's model results increased significantly as fibrosis advanced (analysis of variance test: P < 0.001). FibroQ trended to be superior in predicting signifi cant fi brosis score in chronic hepatitis C compared with other noninvasive tests. CONCLUSION: FibroQ is a simple and useful test for predicting signifi cant fi brosis in patients with chronic hepatitis C.

关键词： Liver fibrosis Noninvasive test FibroQ Aspartate aminotransferase, Alanine aminotransferase FIB-4 index Aspartate aminotransferase to plateletratio index Lok's model Cirrhosis discriminant score Pohl score

激光增材制造沉淀强化镍基高温合金热裂纹研究进展

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表面技术 2024年第7期53卷 1-14页

作者：李尧寇浩南李梦阳张阔刘源虢婷张凤英宋绪丁长安大学材料科学与工程学院西安710064 长安大学道路施工技术与装备教育部重点实验室西安710064

针对沉淀强化镍基高温合金中的裂纹现象,对比了2类热裂纹(即凝固裂纹和液化裂纹)的典型特征、形成位置和条件。从枝晶生长、元素偏析、强化相析出、固态相变和残余应力应变等角度,系统综述了热裂纹的形成机理和热裂纹敏感性影响因素。... 详细信息

针对沉淀强化镍基高温合金中的裂纹现象,对比了2类热裂纹(即凝固裂纹和液化裂纹)的典型特征、形成位置和条件。从枝晶生长、元素偏析、强化相析出、固态相变和残余应力应变等角度,系统综述了热裂纹的形成机理和热裂纹敏感性影响因素。在此基础上,从合金成分调控、工艺参数优化、基板预热以及热等静压处理等方面,概述了增材制造高温合金热裂纹调控和抑制的主要措施。最后,针对激光增材制造沉淀强化镍基高温合金热裂纹研究中存在的问题,提出了进一步研究和发展的建议,为实现无裂纹镍基高温合金的增材制造提供了参考。

关键词：激光增材制造沉淀强化镍基高温合金开裂机理热裂纹敏感性裂纹抑制

A plant sesquiterpene lactone and its derivative reduce cutaneous side effect of vemurafenib,a BRAF inhibitor drug to treat late stage melanoma

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中国药理学与毒理学杂志 2015年第S1期29卷 90-91页

作者： Meng-ting chang Jia-hua FENG Kyoko NAKAGAWA-GOTO kuo-Hsiung LEE Lie-Fen SHYUR Agricultural Biotechnology Research Center Academia Sinica Chinese Taipei 115 Institute of Plant Biology College of Life Science National Taiwan University Chinese Taipei 106 College of Medical Pharmaceutical and Health Sciences Kanazawa University Kakuma-machi Kanazawa 920-1192 Japan Natural Products Research Laboratories Eshelamn School of Phamacy University of North Carclina Chape Hill North Carolina 27599 USA

OBJECTIVE To investigate the pharmacological effect of a plant sesquiterpene lactone(designated D)and its semi-organically synthesized novel derivative(designated S)and the role of lipid mediators,viz.,oxylipins in attenuating vemurafenib-induced cutaneous side *** A DMBA/TPAinduced skin carcinogenesis mouse model mimicking cutaneous side effect caused by vemurafenib was established to evaluate the efficacy of compound D and S in reversal of vemurafenib side *** oxylipin metabolomics platform using UPLC-TQD mass spectrometry coupled with partial least squares-discriminant analysis(PLS-DA)analysis,cell-based assays,and immunochemistry analysis were performed to elucidate the mechanism insights of DET and S compounds and the role of specific oxylipins in skin cancer *** Vemurafenib treatment expedited the skin papillomas formation in DMBA-TPA treated mouse from week 6 to week *** D and S compounds could suppress the vemurafenib side effect and also decrease total papillomas numbers(55% to 72%)and average sizes(66% to 89%).Oxylipins metabolome analysis shows that specific arachidonic acid metabolites may play a role in vemurafenib-induced squamous cell carcinoma or keratoacanthomas formation in mouse skin that can be deregulated by D or S compound ***,S compound can inhibit vemurafenib-induced paradoxical activation of MAP kinases in mouse skin or in NRAS mutant melanoma *** Our results indicate that plant sesquiterpene lactone D and its novel analog can reduce cutaneous side effect of vemurafenib through novel modes of action by inhibiting paradoxical activation of MAP kinases and de-regulating pro-inflammation mediators COX-2 and specific ecosanoid-type of *** study may suggest a novel adjuvant therapy approach in treatment of BRAFV600 Emutant melanoma.

关键词： vemurafenib sesquiterpene lactone two-stage carcin

Thermoelastic Problem of a Long Annular Multilayered Cylinder

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World Journal of Mechanics 2013年第5期3卷 6-12页

作者： Yi Hsien Wu kuo-chang Jane Department of Applied Mathematics National Chung Hsing University Taichung Chinese Taipei Department of Information Management Oriental Institute of Technology Taipei Chinese Taipei

Thermoelastic transient response of multilayered annular cylinders of infinite lengths subjected to known inner pressure and outer surfaces cooling are considered. A method based on the Laplace transformation and finite difference method has been developed to analyze the thermoelasticity problem. Using the Laplace transform with respect to time, the general solutions of the governing equations are obtained in transform domain. The solution is obtained by using the matrix similarity transformation and inverse Laplace transform. Solutions for the temperature and thermal stress distributions in a transient state were obtained. It was found that the temperature distribution, the displacement and the thermal stresses change slightly as time increases.

关键词： Thermoelastic Multilayered Annular Cylinders Laplace Transformation Finite Difference Method

Integrated Movable System of Fuel Cell with Replaceable Fiber Bipolar Plate

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Smart Grid and Renewable Energy 2011年第4期2卷 399-409页

作者： chang Shiuh Ming kuo Jenn Kun 不详

It is important for the fuel cell integrated movable system to operate voltage and current using safety control technology. In order to work at the convenient condition of the fuel cell system, high performance fuel cell stack with replaceable fiber bipolar plate should be arranged with the integrated subsystem and appropriate working process. The parameters which affect the performance of PEMFC consisting of relative humidity, reaction temperature, gas inlet temperature, gas inlet pressure, and hydrogen and air flow rate. This study is to develop the integrated movable system on distributed power generation and backup power application, such as oxidant supply system, fuel supply system, heat management system, water management system, and power conditioning system. It comprises a novel PLC (Programmable Logic Control) system and human-machine interface. The controller is developed to control fuel cell system and record the operation data by using data acquisition system. The controller can be applied to high performance stack and system to obtain the best performance. The easy-taken high capacity hydrogen barrel embedded into steel plate of this movable system and more convenient than other fuel cell system.

关键词： Movable System Fiber Bipolar Plate PEMFC PLC Control System Touch Panel Easy-Taken

An MDL approach to efficiently discover communities in bipartite network

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Journal of Central South University 2014年第4期21卷 1353-1367页

作者：徐开阔曾春秋元昌安李川唐常杰 College of Computer Science & Technology Chengdu University of Information Technology School of Computing and Information Sciences Florida International University Guangxi Teachers Education University School of Computer Science Sichuan University

An minimum description length(MDL) criterion is proposed to choose a good partition for a bipartite network. A heuristic algorithm based on combination theory is presented to approach the optimal partition. As the heuristic algorithm automatically searches for the number of partitions, no user intervention is required. Finally, experiments are conducted on various datasets, and the results show that our method generates higher quality results than the state-of-art methods, cross-association and bipartite, recursively induced modules. Experiment results also show the good scalability of the proposed algorithm. The method is applied to traditional Chinese medicine(TCM) formula and Chinese herbal network whose community structure is not well known, and found that it detects significant and it is informative community division.

关键词： community detection bipartite network minimum description length

Analysis on the Industrial Development Plan of the Tribal Hot Spring Demonstration Area in Taiwan’s Indigenous Areas

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Open Journal of Applied Sciences 2021年第1期11卷 58-71页

作者： hsiao-Ming chang Chin-Lung Chou Mao-Chun Chiu School of Physical Education Putian University Putian China Department of Tourism Leisure and Health Ching Kuo Institute of Management and Health Keelung Taiwan China Department of Recreational and Sport Management Tajen University Pingdong Taiwan China

Under the impact of modern life, the traditional culture of Taiwan’s indigenous tribes is not only affected, because the traditional life is not easy and employment is difficult, which also causes the outflow of tribal population. Therefore, after the establishment of the Taiwan Council of indigenous people (TCIP), tourism development has been listed as an important policy, and it wants to use tourism development to improve the lives of indigenous people and enhance the ability of tribal economic development. The purpose of this study is to analyze the advantages and disadvantages of TCIP in developing tribal hot spring demonstration areas in indigenous areas. This study adopts the method of case study to investigate by means of document analysis, field interview and observation. The results of SWOT analysis show that: 1) Advantages: the indigenous tribes are rich in natural resources and have unique cultural characteristics. 2) Weakness: when indigenous people invest in hot spring industry, they will face difficulties in obtaining development funds, lack of land use and hot spring management talents. 3) Opportunities: Because of the prevailing leisure culture in Taiwan, coupled with the relevant laws and policies and plans of the indigenous people, it is guaranteed that the indigenous people can participate in the development plan of the hot spring demonstration area. 4) Threats: The development of the existing hot springs in the indigenous villages and towns is mostly invested by the Han people, so it is difficult for the indigenous people to compete with them. Moreover, they lack the management ability and guidance to enter the competitive market of hot spring development.

关键词： Hot Spring Tribal Tourism Industrial Development Indigenous

Diagnostic capability of peripapillary retinal nerve fiber layer parameters in time-domain versus spectral-domain optical coherence tomography for assessing glaucoma in high myopia

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International Journal of Ophthalmology(English edition) 2017年第7期10卷 1106-1112页

作者： Mei-Ching Teng Yi-Chieh Poon kuo-Chi Hung Hsueh-Wen chang Ing-Chou Lai Jen-Chia Tsai Pei-Wen Lin Chien-Yun Wu Chueh-Tan Chen Pei-chang Wu Department of Ophthalmology Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of MedicineNiaosong Dist Department of Biological Sciences National Sun Yat-Sen University

AIM： To evaluate and compare the diagnostic capabilities of peripapillary retinal nerve fiber layer（p-RNFL） parameters of Spectralis optical coherence tomography（OCT） versus Stratus OCT to detect glaucoma in patients with high myopia. METHODS： This is a retrospective, cross-sectional study. Sixty highly myopic eyes of 60 patients were enrolled, with 30 eyes in the glaucoma group and 30 eyes in the control group. All eyes received peripapillary imaging of the optic disc using Stratus and Spectralis OCT. Areas under the receiver operating characteristic curve（AUROC） and the sensitivity at specificity of 〉80% and 〉95% for p-RNFL parameters obtained using the two devices to diagnose glaucoma were analysed and compared. RESULTS： In Spectralis OCT, p-RNFL thickness parameters with the largest AUROC were the temporal-inferior sector（0.974） and the inferior quadrant（0.951）, whereas in Stratus OCT, the best parameters were the 7-o＇clock sector（0.918） and the inferior quadrant（0.918）. Compared to the Stratus OCT parameters, the Spectralis OCT parameters demonstrated generally higher AUROC; however, the difference was not statistically significant. CONCLUSION： The best p-RNFL parameters for diagnosing glaucoma in patients with high myopia were the temporal-inferior sector on Spectralis OCT and the 7-o＇clock sector on Stratus OCT. There were no significant differences between the AUROCs for Spectralis OCT and Stratus OCT, which suggest that the glaucoma diagnostic capabilities of these two devices in patients with high myopia are similar.

关键词： diagnostic capability glaucoma high myopia optical coherence tomography retinal nerve fiber layer