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Dibutyl phthalate disrupts[Ca^(2+)]_(i),reactive oxygen species,[pH]_(i),protein kinases and mitochondrial activity,impairing sperm function

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Journal of Environmental Sciences 2025年第5期151卷 68-78页

作者： Seung Hyun park Myung chan Gye Department of Life Science and Institute for Natural Sciences Hanyang UniversitySeoul 04763Republic of Korea

To explore the mechanism of sperm dysfunction caused by dibutyl phthalate(DBP),the effects of DBP on intracellular[Ca^(2+)]and[pH],reactive oxygen species(ROS),lipid peroxidation(LPO),mitochondrial permeability transition pore(mPTP)opening,mitochondrial membrane potential(MMP),adenosine triphosphate(ATP)levels,phosphorylation of protein kinase A(PKA)substrate proteins and phosphotyrosine(p-Tyr)proteins,sperm motility,spontaneous acrosome reaction,and tail bending were examined in mouse *** 100μg/mL,DBP significantly increased tail bending and[Ca^(2+)]***,DBP showed biphasic effects on[pH]*** at 10–100μg/mL significantly decreased sperm ***,Ca^(2+)ionophore A23187 decreased[pH]_(i)sperm motility,suggesting that DBP-induced excessive[Ca^(2+)]_(i)decreased sperm *** significantly increased ROS and *** at 100μg/mL significantly decreased mPTP closing,MMP,and ATP levels in spermatozoa,as did H2O2,indicative of ROS-mediatedmitochondrial dysfunction caused by *** as well as H2O2 increased p-Tyr sperm proteins and phosphorylated PKA substrate sperm *** at 1–10μg/mL significantly increased the spontaneous acrosome reaction,suggesting that DBP can activate sperm ***,DBP showed a biphasic effect on intracellular signaling in *** concentrations relevant to seminal ortho-phthalate levels,DBP activates[pH]i,protein tyrosine kinases and PKA via physiological levels of ROS generation,potentiating sperm *** at high doses excessively raises[Ca^(2+)]_(i)and ROS and disrupts[pH]i,impairing the mitochondrial function,tail structural integrity,and sperm motility.

关键词： Dibutyl phthalate Reactive oxygen species(ROS) [Ca^(2+)]_(i) [pH]_(i) Mitochondria Protein kinases Sperm

Predicting tumor response after preoperative chemoradiation using clinical parameters in rectal cancer

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World Journal of Gastroenterology 2011年第48期17卷 5310-5316页

作者： chan ho park Hee Cheol Kim Yong Beom Cho Seong Hyeon Yun Woo Yong Lee Young Suk park Doo ho Choi ho-Kyung Chun Departmentof Surgery Samsung Medical Center Sungkyunkwan Univer-sity School of Medicine Irwon-dong 50 Gangnam-gu Seoul135-710 South Korea Division of Hematology-Oncology Depart-ment of Medicine Samsung Medical Center Sungkyunkwan University School of Medicine Irwon-dong 50 Gangnam-guSeoul 135-710 South Korea Department of Radiation Oncology SamsungMedical Center Sungkyunkwan University School of MedicineIrwon-dong 50 Gangnam-gu Seoul 135-710 South Korea

AIM: To evaluate the clinical parameters and identify a better method of predicting pathological complete response (pCR). METhoDS: We enrolled 249 patients from a database of 544 consecutive rectal cancer patients who underwent surgical resection after preoperative chemoradiation therapy (PCRT). A retrospective review of morphological characteristics was then performed to collect data regarding rectal examination findings. A scoring model to predict pCR was then created. To validate the ability of the scoring model to predict complete ***: Seventy patients (12.9%) achieved a pCR. A multivariate analysis found that pre-CRT movability (P = 0.024), post-CRT size (P = 0.018), post-CRT morphology (P = 0.023), and gross change (P = 0.009) were independent predictors of pCR. The accuracy of the scoring model was 76.8% for predicting pCR with the threshold set at 4.5. In the validation set, the accuracy was 86.7%. CONCLUSION: Gross changes and morphological findings are important predictors of pathological response. Accordingly, PCRT response is best predicted by a combination of clinical, laboratory and metabolic information.

关键词： Rectal cancer Preoperative chemoradiotherapy Downstaging Tumor regression Validation

Classification, Expression Pattern, and E3 Ligase Activity Assay of Rice U-Box-Containing Proteins

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Molecular Plant 2008年第5期1卷 800-815页

作者： Li-Rong Zenga chan ho park R.C. Venu Julian Gough Guo-Liang Wang Department of Plant Pathology Plant Molecular Biology and Biotechnology Program The Ohio State University 2021 Coffey Rd 201 Kottman Hall Columbus OH 43210 USA Present address: Boyce Thompson Institute for Plant Research Tower Rd Ithaca NY 14853 USA Department of Computer Science University of Bristol Woodland Rd Bristol BS8 1UB UK Crop Gene Engineering Key Laboratory of Hunan Province Hunan Agricultural University Changsha Hunan 410128 China

Ubiquitin ligases play a central role in determining the specificity of the ubiquitination system by selecting a myriad of appropriate candidate proteins for modification. The U-box is a recently identified, ubiquitin ligase activityrelated protein domain that shows greater presence in plants than in other organisms. In this study, we identified 77 putative U-box proteins from the rice genome using a battery of whole genome analysis algorithms. Most of the U-box protein genes are expressed, as supported by the identification of their corresponding expressed sequence tags （ESTs）, full-length cDNAs, or massively parallel signature sequencing （MPSS） tags. Using the same algorithms, we identified 61 U-box proteins from the Arabidopsis genome. The rice and Arabidopsis U-box proteins were classified into nine major classes based on their domain compositions. Comparison between rice and Arabidopsis U-box proteins indicates that the majority of rice and Arabidopsis U-box proteins have the same domain organizations. The inferred phylogeny established the homology between rice and Arabidopsis U-box/ARM proteins. Cell death assay using the rice protoplast system suggests that one rice U-box gene, OsPUB51, might act as a negative regulator of cell death signaling. In addition, the selected U-box proteins were found to be functional E3 ubiquitin ligases. The identification and analysis of rice U-box proteins hereby at the genomic level will help functionally characterize this class of E3 ubiquitin ligase in the future.

关键词：表达的序列标记蛋白质类泛素泛素蛋白连接酶类基因基因组拟南芥属稻属

Liver transplantation for hepatocellular carcinoma: Current status in hong Kong, China

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Hepatobiliary & Pancreatic Diseases International 2025年第1期24卷 45-49页

作者： Karin ho Albert chan Liver Transplant Center Queen Mary HospitalDepartment of SurgeryThe University of Hong KongHong KongChina

With the advances in transplant oncology in recent years, the role of liver transplantation has expanded to make curative treatment a possibility for a wider patient population. We highlight strategies in hong Kong, China that have enabled preoperative prognostication for judicious patient selection, downstaging therapy to definitive treatment, and postoperative therapies that have provided a growing role for liver transplantation in patients with more advanced hepatocellular carcinoma.

关键词： Living donor liver transplantation Downstaging Conversion therapy Transplant oncology

Two VOZ transcription factors link an E3 ligase and an NLR immune receptor to modulate immunity in rice

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Molecular Plant 2021年第2期14卷 253-266页

作者： Jiyang Wang Ruyi Wang hong Fang Chongyang Zhang Fan Zhang Zeyun Hao Xiaoman You Xuetao Shi chan ho park Kangyu Hua Feng He Maria Bellizzi Kieu Thi Xuan Vo Jong-Seong Jeon Yuese Ning Guo-Liang Wang State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant ProtectionChinese Academy of Agricultural SciencesBeijing 100193China Department of Plant Pathology Ohio State UniversityColumbusOH 43210USA Graduate School of Biotechnology and Crop Biotech Institute Kyung Hee UniversityYongin 17104Korea

Nucleotide-binding leucine-rich repeat(NLR)proteins play critical roles in plant ***,how NLRs are regulated and activate defense signaling is not fully *** rice(Oryza sativa)NLR receptor Piz-t confers broad-spectrum resistance to the fungal pathogen Magnaporthe oryzae and the RING-type E3 ligase AVRPIZ-T INTERACTING PROTEIN 10(APIP10)negatively regulates Piz-t *** this study,we found that APIP10 interacts with two rice transcription factors,VASCULAR PLANT ONEZINC FINGER 1(OsVOZ1)and OsVOZ2,and promotes their degradation through the 26S proteasome ***1 displays transcriptional repression activity while OsVOZ2 confers transcriptional activation activity in *** osvoz1 and osvoz2 single mutants display modest but opposite *** resistance in the non-Piz-t ***,the osvoz1 osvoz2 double mutant exhibits strong dwarfism and cell death,and silencing of both genes via RNA interference also leads to dwarfism,mild cell death,and enhanced resistance to *** in the non-Piz-t *** OsVOZ1 and OsVOZ2 interact with *** silencing of OsVOZ1 and OsVOZ2 in the Piz-t background decreases Piz-t protein accumulation and transcription,reactive oxygen species-dependent cell death,and resistance to *** containing *** together,these results indicate that OsVOZ1 and OsVOZ2 negatively regulate basal defense but contribute positively to Piz-t-mediated immunity.

关键词： rice E3 ligase VOZ transcription factor NLR protein fungal pathogen Magnaporthe oryzae cell death

Oligomerization between BSU1 Family Members Potentiates Brassinosteroid Signaling in Arabidopsis

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Molecular Plant 2016年第1期9卷 178-181页

作者： Eun-Ji Kim Ji-Hyun Youn chan-ho park Tae-Woo Kim Shenheng Guan Shouling Xu AlmaL. Burlingame Young-Pil Kim Seong-Ki Kim Zhi-Yong Wang Tae-Wuk Kim Research Institute for Natural Sciences Hanyang University Seoul 133-791 Korea

Brassinosteroid （BR） regulates diverse physiological and devel- opmental processes in plants （Choudhary et al., 2012）. BR binds to the receptor kinase BRI1 to trigger a phosphorylation/ dephosphorylation cascade, which includes phosphorylation of Brassinosteroid Signaling Kinase 1 （BSK1） and Constitutive Differential Growth 1 （CDG1） by BRI1, phosphorylation of BSU1 by CDG1, and dephosphorylation of BIN2 （a GSK3-1ike kinase） by BSU1 （Kim et al., 2009, 2011; Wang et al., 2014）. Almost all the downstream components of BR signaling are encoded by multiple genes of a family.

关键词： RESEARCH Oligomerization Brassinosteroids Arabidopsis

Dissection of the molecular mechanism underlying AvrPiz-t-Piz-t mediated immunity against Magnaporthe oryzae

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Dissection of the molecular mechanism underlying AvrPiz-t-Pi...

OsELF3-2, an Ortholog of Arabidopsis ELF3, Interacts with the E3 Ligase APIP6 and Negatively Regulates Immunity against Magnaporthe oryzae in Rice

2018全国植物生物学大会

作者： Yuese Ning Shi Xuetao Wang Ruyi chan ho park Guo-Liang Wang State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant ProtectionChinese Academy of Agricultural Sciences Department of Plant Pathology Ohio State University

Rice blast,caused by the fungal pathogen Magnaporthe oryzae,is one of the most serious rice *** previously cloned the broad-spectrum resistance(R) gene Piz-t in rice and its cognate Avr gene AvrPiz-t in *** elucidate the molecular mechanism underlying AvrPiz-t-Piz-t mediated rice immunity against ***,we performed a yeast twohybridscreen to identify potential host targets of AvrPiz-t in *** the twelveAPIPs(AvrPiz-t Interacting Proteins),APIP5 is a bZIP-type transcription factor,APIP6 is aRING-type E3 ligase,and APIP7 is the potassium channel OsAKT1 *** demonstrated that AvrPiz-t targets APIP5,APIP6 andOsAKT1 for suppression of the PAMP-triggered immunity(PTI) in *** addition,APIP6 ubiquitinates and degrades OsELF3-2 in *** also found thatAPIP5 positively regulates the accumulation of ***,Piz-t can stabilize APIP5 to prevent necrosis at the necrotrophic *** together,these results indicate that the fungal effector AvrPiz-t targets multiple rice proteins that play important roles in both PTI and effector-triggered immunity.

关键词： Magnaporthe oryzae transcription factor APIP5 ubiquitin E3 ligase APIP6 potassium channel OsAKT1 Piz-t

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Molecular Plant 2015年第11期8卷 1679-1682页

作者： Yuese Ning Xuetao Shi Ruyi Wang Jiangbo Fan chan ho park Chongyang Zhang Ting Zhang Xinhao Ouyang Shigui Li Guo-Liang Wang State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant Protection Chinese Academy of Agricultural Sciences Beijing 100193 China Department of Plant Pathology Ohio State University Columbus OH 43210 USA Rice Genetics and Breeding Rice Research InstituteSichuan Agricultural University Chengdu Sichuan 611130 China

Dear Editor, The ubiquitin proteasome system （UPS） is a highly regulated mechanism of intracellular protein degradation in eukaryotes. The UPS comprises three key enzymes： ubiquitin-activating enzyme E1, ubiquitin-conjugating enzyme E2, and ubiquitin ligase enzyme E3. There are nearly 1500 E3 ligase genes in rice and Arabidopsis genomes that target substrates for many biological processes for plant growth and tolerance to abiotic and biotic stresses.

关键词：蛋白酶体内肽酶复合物稻温病菌泛素泛素蛋白连接酶类泛素激活酶类泛素缀合酶类拟南芥属稻属

RGB Color-Discriminable Photonic Synapse for Neuromorphic Vision System

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Nano-Micro Letters 2025年第4期17卷 39-62页

作者： Bum ho Jeong Jaewon Lee Miju Ku Jongmin Lee Dohyung Kim Seokhyun Ham Kyu-Tae Lee Young-Beom Kim Hui Joon park Department of Organic and Nano Engineering&Human-Tech Convergence Program Hanyang UniversitySeoul 04763Korea Department of Semiconductor Engineering Hanyang UniversitySeoul 04763Korea Department of Mechanical Engineering Hanyang UniversitySeoul 04763Korea Department of Physics Inha UniversityIncheon 22212Korea

To emulate the functionality of the human retina and achieve a neuromorphic visual system,the development of a photonic synapse capable of multispectral color discrimination is of paramount ***,attaining robust color discrimination across a wide intensity range,even irrespective of medium limitations in the channel layer,poses a significant ***,we propose an approach that can bestow the color-discriminating synaptic functionality upon a three-terminal transistor flash memory even with enhanced discriminating *** incorporating the strong induced dipole moment effect at the excitation,modulated by the wavelength of the incident light,into the floating gate,we achieve outstanding RGB color-discriminating synaptic functionality within a remarkable intensity range spanning from 0.05 to 40 mW cm^(-2).This approach is not restricted to a specific medium in the channel layer,thereby enhancing its *** effectiveness of this color-discriminating synaptic functionality is demonstrated through visual pre-processing of a photonic synapse array,involving the differentiation of RGB channels and the enhancement of image contrast with noise ***,a convolutional neural network can achieve an impressive inference accuracy of over 94%for Canadian-Institute-For-Advanced-Research-10 colorful image recognition task after the *** proposed approach offers a promising solution for achieving robust and versatile RGB color discrimination in photonic synapses,enabling significant advancements in artificial visual systems.

关键词： Organic field-effect transistor Photonic synapse Excited-state dipole moment RGB color discrimination Neuromorphic visual system