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Asian Pacific Journal of Tropical Medicine 2014年第3期7卷 207-211页

作者： Anawat Phalee chalobol wongsawad Department of Biology Faculty of ScienceChiang Mai University Applied Technology in Biodiversity Research Unit.Institute of Science and Technology Chiang Mai University

Objective:To investigate the infection of Fasciola gigantka(***)in domestic cattle from Chiang Mai province and molecular confirmation using ITS-2 ***:The liver and gall bladder of Bubalus bubalis(***)and Bos taurus(***)from slaughterhouses were examined adult worms and prevalence *** species confirmation with phylogenetic analysis using ITS-2 sequences was performed by maximum likelihood and UPGMA ***:The total prevalences of infection in *** and Bubalus taurus(***)were67.27%and 52.94%*** respective prevalence in both *** and *** were acquired from Doi-Saket,Muang,and Sanpatong districts,with 81.25%,62.50%and 60.00%for *** and 62.50%,50.00%and 47.06%for Bos taunts *** species confirmation of *** and some related species by basing on maximum likelihood and UPGMA methods used,4 groups of trematodes were generated,first *** group including specimen of Chiang Mai,second 2 samples of ***,third group of 3 rumen flukes;Orthocoelium streptocoelium,*** and Paramphistomum epliclitum and fourth group of 3 minute intestinal flukes:Haplorchis taichui,Stellantchasmu *** *** liver fluke;Opisthorchis civerrini ***:These results can be confirmed the Giant liver fluke which mainly caused fascioliasis in Chiang Mai was identified as *** and specimens were the same as those of *** recorded in olher different *** sequence of ITS-2 region has been proven as effective diagnostic tool for the identification of ***.

关键词： Fasciola gigantica Domestic cattle Chiang Mai Molecular confirmation ITS-2 region

Phylogenetic analysis reveals cryptic species diversity within minute intestinal fluke,Stellantchasmus falcatus Onji and Nishio,1916(Trematoda,Heterophyidae)

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Asian Pacific Journal of Tropical Medicine 2017年第2期10卷 159-163页

作者： chalobol wongsawad Nattawadee Nantarat Pheravut wongsawad Department of Biology Faculty of ScienceChiang Mai UniversityChiang Mai 50202Thailand Applied Technology for Biodiversity Institute for Science and TechnologyChiang Mai UniversityChiang Mai 50202Thailand Economic Plant Genome Service Centre Faculty of ScienceChiang Mai UniversityChiang Mai 50202Thailand

Objective:To examine the taxonomic boundaries in the Stellantchasmus populations in different hosts and reveal the cryptic speciation of the Stellantchasmus in Thailand based on both morphological and molecular ***:Stellantchasmus falcatus(***) *** collected from different hosts throughout *** worms were examined and *** nuclear ITS2 gene and the mitochondrial COI gene were used to investigate the *** *** Dermogenus pusillus and Liza ***:Stellantchasmus was one of food-borne trematode that widely distribute throughout *** comparison of *** *** Dermogenus pusillus and Liza subviridis indicated a genetic divergence of nuD NA with 3.6% and mtD NA with 19.3%,*** characteristics were fairly different in the ratio of body size,length of prepharynx,and ratio of organ ***:Our results provide initial evidence that *** *** different hosts tend to be a different species based on both molecular and morphological *** species complexes are generally found among parasites that tend to have large populations and/or rapid *** degree of genetic diversity existing would suggest the practice of targeted regimes to design and minimize these lections of anthelmintic resistance in the future.

关键词： Cryptic species Trematode Host-parasite relationship Speciation Stellantchasmus

Morphological study and molecular epidemiology of Anisakis larvae in mackerel fish

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Asian Pacific Journal of Tropical Medicine 2021年第5期14卷 214-222页

作者： Vipavinee Cheypanya Pheravut wongsawad chalobol wongsawad Nattawadee Nantarat Graduate Master's Degree Program in Biology Faculty of ScienceChiang Mai UniversityMueang50200Thailand Department of Biology Faculty of ScienceChiang Mai UniversityMueang50200Thailand Research Center in Bioresources for Agriculture Industry and MedicineFaculty of ScienceChiang Mai UniversityChiang Mai 50200Thailand Economic Plant Genome Service Centre Faculty of ScienceChiang Mai UniversityChiang Mai 50200Thailand Environmental Science Research Center(ESRC) Chiang Mai UniversityChiang Mai50200Thailand

Objective:To examine and study the morphology,epidemiology,and molecular phylogeny of Anisakis larvae in blue mackerel[Scomber australasicus(Cuvier,1832)]and Indian mackerel[Rastrelliger kanagurta(Cuvier,1816)]using light microscope,scanning electron microscope,molecular phylogeny,and species delimitation methods for confirmation and investigation of Anisakis species and their evolutionary ***:A total of 90 fish(45 per species)were purchased from a department store in Chiang Mai,*** samples were investigated for morphological characteristics using light and scanning electron *** phylogeny and species delimitation methods based on the cox2 gene were ***:The prevalence,mean intensity(Mean±SEM),and mean abundance of Anisakis larvae(Mean±SEM)in blue mackerel were 77.78%,6.74±1.320,and 5.24±1.107,respectively,and in Indian mackerel,these values were 13.33%,2.50±0.764,and 0.33±0.159,*** electron microscopy showed the detail of morphological characteristics and provided the different shapes of mucron and excretory pores in Anisakis larvae congruent with the phylogenetic *** species tree was congruent with the phylogenetic ***:The prevalence,mean intensity,and mean abundance of Anisakis larvae were higher in blue *** the best of our knowledge,this is the first time that Anisakis pegreffii was found in blue mackerel in *** phylogenetic tree also supported the morphological data of Anisakis ***,species delimitation based on cox2 revealed 1-3 possible cryptic species in this *** *** of fish products is unpleasant and a health concern considering human infection with larvae(anisakiasis)arises.

关键词： Anisakis Mackerel Thailand Phylogenetic tree Taxonomy Species delimitation

Epidemiology and molecular genotyping of echinostome metacercariae in Filopaludina snails in Lamphun Province,Thailand

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Asian Pacific Journal of Tropical Medicine 2014年第1期7卷 26-29页

作者： Waraporn Noikong chalobol wongsawad Applied Parasitology Research Laboratory Department of Biology Faculty of ScienceChiang Mai University Applied Biology Program Faculty of Science and TechnologyPibulsongkram Rajabhat University Applied Technology in Biodiversity Research Unit Institute of Science and TechnologyChiang Mai University

Objective:To analyze the prevalence of echinostome metacercariae in Filopaludina dorliaris(***)and Filopaludina martensi martensi(*** martensi)and genotype variation of echinostome metacercariae by using random amplified polymorphic DNA(RAPD)***:Filopaludina *** were collected from eight localities of Lamphun Province,Northern Thailand and examined for echinostome ***-PCR was used lo analyze genotype variation of echinostome ***:A total of 3 226 *** and *** martensi snails were collected from eight *** overall prevalences of echinostome metacercariae in *** and *** martensi were 40.89%and 36.27%,while the intensity of infection was 20.37 and 12.04,*** dendrogram constructed base on RAPD profiles,4 well supported domains were generated;(i)group of metacercariae from Ban Hong,Mae Ta,Meaung,Pa Sang,Toong Hua Chang,and Weang Nong that were clustered in the group of ***,(ii)Ban Thi,(iii)Lee,and(iv)3 adults of an out ***:This research demonstrated RAPD profiling has been a useful tool to detect DNA polymorphisms to determine genetic relationship between echinostome metacercariae in Lamphun Province,Northern Thailand.

关键词： Echinostoma sp. Filopaludina sp.snails RAPD-PCR Epidemiology Metacercariae

Investigation of cryptic diversity and occurrence of echinostome metacercariae infection in Anentome helena (von dem Busch, 1847)

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Asian Pacific Journal of Tropical Medicine 2018年第10期11卷 590-596页

作者： Nithinan Chomchoei chalobol wongsawad Nattawadee Nantarat Department of Biology Faculty of Science Chiang Mai University Graduate School Chiang Mai University Applied Parasitology Research Laboratory Department of Biology Faculty of Science Chiang Mai University Environmental Science Research Center Faculty of Science Chiang Mai University Science and Technology Research Institute Chiang Mai University

Objective: To investigate the parasitic infection of Anentome helena(A. helena) and determine the validity of species boundaries for A. helena by combining molecular phylogeny and morphological approaches. Methods: A total of 325 individuals of A. helena were collected throughout northern Thailand. Shells were measured and compared by t-test. Radulae were investigated by using light and scanning electron microscope. Two partial mitochondrial DNA sequences of COI and 16S rRNA from 36 specimens of A. helena and related species were used to test the validity of the morphospecies. Phylogenetic trees were constructed using neighbour joining, maximum likelihood and Bayesian inference. Infection of A. helena with trematode larva was examined and observed. Results: Morphological examination of A. helena revealed 2 distinct morphospecies. Genetic divergences supported the separation of the two morphotypes into two distinct groups. Both individual and combined analyses of the two nucleotide fragments revealed two phylogroups that corresponded with shell and radula characteristics. In addition, A. helena was found infected with 37-collar spined echinostome metacercariae. The prevalence and intensity of metacercariae was highest in the San Kamphaeng district, Chiang Mai province, with 7.5% and 1.670±0.577, at comprehensive taxonomic respectively. Conclusions: These findings suggest thision of this unrecognised species complex is needed. This study represents the preliminary step to reveal new data on the recent distribution of trematode infection in A. helena. This information may be useful for developing conservation management of the snail and the practice of targeted regimes to reduce anthelmintic resistance in the future.

关键词： Phylogeny Freshwater snail Echinostomes Biodiversity Anentome

Epidemiology of cercarial stage of trematodes in freshwater snails from Chiang Mai province,Thailand

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Asian Pacific Journal of Tropical Biomedicine 2013年第3期3卷 237-243页

作者： Thapana Chontananarth chalobol wongsawad Applied Parasitology Research Laboratory Department of BiologyFaculty of ScienceChiang Mai University Applied Technology in Biodiversity Research Unit Institute for Science and Technology ResearchChiang Mai University

Objective:To investigate the epidemiological situation of cercarial trematodes infection in freshwater snails from different water resources in Chiang Mai province,***:The snail specimens were collected from 13 districts of Chiang Mai province during April 2008 to February *** prevalence of cercarial infection in snails was investigated using the crushing *** drawing was done with the help of a camera lucida for the morphological ***:A total of 2479 snail individuals were collected and classified into 7 families,11 genera,and 14 species,Among them,8 snails species were found to be infected with an overall prevalence of 17.27%(428/2479),which infected with nine groups of cercariae;gymnocephalous cercaria,strigea cercaria,megalurous cercaria,monostome cercaria,parapleurolophocercous cercaria(Haplorchis cercaria),pleurolophocercous cercaria,furcocercous cercaria(Transversotrema cercaria),xiphidiocercaria,and virgulate *** parapleurolophocercous cercaria was found to be the dominant type among the cercarial infection in the snails(64.25%).Conclusions:The various species of snails found in the research location act as the intermediate hosts for the high prevalence of parasitic infection of many species of *** work will provide new information on both the distribution and first intermediate host of trematodes.

关键词： Chiang Mai Trematodes Cercariae Pleurolophocercous Prevalence Freshwater snails

Molecular phylogeny of trematodes in Family Heterophyidae based on mitochondrial cytochrome c oxidase subunit Ⅰ(mCO Ⅰ)

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Asian Pacific Journal of Tropical Medicine 2014年第6期7卷 446-450页

作者： Thapana Chontananarth chalobol wongsawad Siriwadee Chomdej Duangduen Krailas Jong Yil Chai Applied Parasitology Research Laboratory Department of BiologyFaculty of Science.Chiang Mai University Department of Biology Faculty of Science Silpakorn University Department of Parasitology and Tropical Medicine Seoul National University College of Medicine Institute of Endemic Diseases Seoul National University Medical Research Center

Objective:To analyze a phylogenetic tree for understanding the molecular systematic of trematode in Pamily *** are highly distributed in ***:Based on thirteen sequences of mitochondrial cytochrome C oxidase subunit 1(mCO I) gene from six genera of heterophyid trernatodes,***,Stellantchasmus,Centrocestus,Metagonimus,Pygidopsis,and Haplnrchoides were aligned automatically using the Clustal × 2.0 program.A phylogenetic tree was constructed by maximum likeiughood(ML) and neighbor-joining(NJ)methods,with 1000 bootstrap using the 5.0 ***:The phylogenetic relationship from both methods was similar and separated into three groups consisting of Haplorchoides pumilio group,Haplorchoides taichui group and another heterophyid ***:The sequence data of mtCOl can he used to investigate the phylogenetic relationships of trernatodes at the genus *** clade of different genera of heterophyid trernatodes can be separated into sister groups that correlated with the morphological characteristic,kind of secondary intermediate host and geographic distribution.

关键词： Phylogenetic relationship mCOI Heterophyidae