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Functionalized CVD monolayer graphene for label-free impedimetric biosensing

Nano Research 2015年第5期8卷 1698-1709页

作者： Shimaa Eissa Gaston Contreras Jimenez Farzaneh Mahvash Abdeladim Guermoune chaker tlili Thomas Szkopek Mohammed Zourob Mohamed Siaj Institut national de la recherche scientifique Centre - Energie Matefiaux et Telecommunications 1650 BouL Lionel Boulet Varennes (Quebec) J3X 1S2 Canada Departemen t de Chimie et Biochimie Universite du Quebec a Montreal Mon trial Quebec H3C 3P8 Canada Dept. ofElectricaland Computer Engineering McGill University Montreal Quebec H3A 2A7 Canada Cranfield Health Vincent Building Cranfield University Cranfield Bedfordshire UK

Recent advances in large area graphene growth have led to many applications in different areas. In the present study, chemical vapor deposited （CVD） monolayer graphene supported on glass substrate electrochemical biosensing applications was examined as electrode material for We report a facile strategy for covalent functionalization of CVD monolayer graphene by electrochemical reduction of carboxyphenyl diazonium salt prepared in situ in acidic aqueous solution. The carboxyphenyl-modified graphene is characterized using Raman spectroscopy, X-ray photoelectron spectroscopy （XPS）, and atomic force microscopy （AFM）, as well as electrochemical impedance spectroscopy （hIS）. We also show that the number of grafted carboxyphenyl groups on the graphene surface can be controlled by the number of cyclic voltammetry （CV） scans used for electrografting. We further present the fabrication and characterization of an immunosensor based on immobilization of ovalbumin antibody on the graphene surface after the activation of the grafted carboxylic groups via EDC/NHS chemistry. The binding between the surface-immobilized antibodies and ovalbumin was then monitored using Faradaic EIS in [Fe（CN）6]^3-/4- solution. The percentage change of charge transfer resistance （Rct） after binding exhibited a linear dependence for ovalbumin concentrations ranging from 1.0 pg·mL^-1 to 100 ng·mL^-1, with a detection limit of 0.9 pg·mL^-1. Our results indicate good sensitivity of the developed functionalized CVD graphene platform, paving the way for using CVD monolayer graphene in a variety of electrochemical biosensing devices.

关键词： CVD graphene electrochemical impedance spectroscopy Biosensor diazoniumfunctionalization

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CRP Binding Kinetics Enhancement Using Local Narrowing into a Bent Channel: Finite Element Analysis

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Engineering（科研） 2022年第1期14卷 62-75页

作者： Mohamed Bahri Imen Dermoul Marshet Getaye Mohamed Amin Elaguech Khouloud Djebbi Mounir Ben Ali chaker tlili Deqiang Wang Chongqing School University of Chinese Academy of Sciences Chongqing China Chongqing Institute of Green and Intelligent Technology Chinese Academy of Sciences Chongqing China University of Chinese Academy of Sciences Beijing China Preparatory Institute for Engineering of Nabeul IPEIN University of Carthage Campus Universitaire Merazka Tunisia NANOMISENE Lab LR16CRMN01 Centre for Research on Microelectronics and Nanotechnology of Sousse Technopole of Sousse Sousse Tunisia University of Sousse Higher Institute of Applied Sciences and Technology of Sousse GREENS-ISSAT Cité Ettafala Sousse Tunisia

Binding kinetics enhancement of a microfluidic biosensor into a micro-channel through the application of a supplementary mechanism has received tremendous attention because of the obtained significant enhancement factor. However, biosensor’s performance enhancement using only simple channel engineering is still rarely realized. Herein, we present a novel design of a complex reactive protein (CRP) biosensor into a U-shaped channel with a sensitive membrane located in the middle of the bent zone. Various critical factors affecting the equilibrium binding time are numerically investigated. The turn geometry is then optimized when the arc length along the inner and outer radii is almost the same, which leads to locally minimizing the channel height overhead the reaction surface and improves the analyte transport towards the sensing area. The numerical studies reveal that applying a local narrowing above the reaction surface can notably enhance the trapping and the surface formation of complex antibody-antigen, thus upgrading the biosensor performance. This work puts a significant advance towards microfluidic channel engineering and the exploration of micro-flow injection experimental studies.

关键词： Finite Elements Method Binding Reaction CRP Biosensor Microfluidic System Microchannel Turn Numerical Simulation

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Development and Evaluation of an Indirect ELISA for the Detection of Mycoplasma hyopneumoniae Natural Infection butnot Inactivated Bacterin Vaccination

Development and Evaluation of an Indirect ELISA for the Dete...

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中国微生物学会兽医微生物学专业委员会暨中国畜牧兽医学会生物制品学分会2021年学术论坛

作者： Yukang Wen Zuobo Xu tlili chaker Yaqin Tian Zhaodi Wang Yaru Ning Honglei Ding Laboratory of Veterinary Mycoplasmology College of Veterinary ScienceSouthwest University Chongqing Institute of Green and Intelligent Technology Chinese Academy of Sciences

Vaccination with inactivated bacterin is the most popular and practical measure to control enzootic *** immunization with inactivated bacterin,*** colonized on the respiratory tract andlungs stimulates the humoral immune responses and produce IgG and IgA *** is a widely used serological method to detect *** ***,commercial IgG ELISA kit cannot distinguish between inactivated bacterin-induced hyperimmune sera and convalescent sera stimulated by natural *** ELISA method is laborious for nasal swab *** of a discriminative ELISA detecting humoral IgG from convalescent sera but not hyperimmune sera facilitates to evaluate the natural infection of *** after inactivated bacterin *** expressed and purified a recombinant protein named Mhp366-N which contains an epitope recognized by the convalescent sera but not hyperimmune *** developed discriminative IgG-ELISA could discriminate between inactivated bacterin-induced hyperimmune sera and convalescent sera,and was reproducible,sensitive,and specific to *** antibody produced by natural *** to sIgA-ELISA method,discriminative IgG-ELISA was more convenient to detect IgG antibody from sera than IgA from nasal swabs,although it has limited sensitivity in the early stages of ***,to some extent,it has a potential to avoid the interference of maternally derivedIgG *** established discriminative IgG-ELISA was efficient to judge the serological IgG antibodies induced from natural infection or inactivated vaccine stimulation and provided a useful method to investigate and evaluate the live organism infection after the application of inactivated bacterin.

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基于电化学还原氧化石墨烯的电化学DNA生物传感器

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传感器与微系统 2019年第6期38卷 75-78页

作者：朱济锋黎学思周大明于乐泳王国东 chaker tlili 河南理工大学电气工程与自动化学院河南焦作454150 中国科学院重庆绿色智能技术研究院精准医疗单分子诊断中心重庆400700 河南理工大学物理与电子信息学院河南焦作454150

使用还原氧化石墨烯(rGO)制备一种简单、快速和可重复方法构建DNA生物传感器。将带负电的氧化石墨烯(GO)与半胱氨酸上带正电的氨基基团通过静电作用相互吸附,用线性扫描伏安法(LSV)电化学还原电极表面吸附的GO。将二茂铁标记的DNA(Fc-D... 详细信息

使用还原氧化石墨烯(rGO)制备一种简单、快速和可重复方法构建DNA生物传感器。将带负电的氧化石墨烯(GO)与半胱氨酸上带正电的氨基基团通过静电作用相互吸附,用线性扫描伏安法(LSV)电化学还原电极表面吸附的GO。将二茂铁标记的DNA(Fc-DNA)探针固定到r GO表面,成功构建DNA传感器。传感器的制备过程使用循环伏安法和拉曼光谱表征。通过杂化前后DNA传感器所展现出方波信号峰电流的差异,实现对目标DNA的定量检测。实验结果表明:目标DNA浓度在1. 0×10^(-13)～1. 0×10^(-6)mol/L范围内,峰电流变化与目标DNA浓度呈线性关系,线性相关系数为0. 981,检测限是2. 0×10^(-13)mol/L (S/N=3)。

关键词：还原氧化石墨烯氨基基团 π-π键 DNA生物传感器

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基于二茂铁标记的电化学DNA生物传感器研究

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传感器与微系统 2019年第5期38卷 55-58页

作者：朱济锋王国东周大明王赟姣梁丽媛 chaker tlili 河南理工大学电气工程与自动化学院河南焦作454150 中国科学院重庆绿色智能技术研究院精准医疗单分子诊断中心重庆400700 河南理工大学物理与电子信息学院河南焦作454150

将二茂铁和巯基修饰的脱氧核糖核酸(DNA)探针通过自组装的方式固定到金电极表面,以二茂铁作为电子介体构建电化学DNA传感器,利用杂化前后DNA传感器所展现出峰电流的差异,实现对目标DNA的定量检测。通过研究DNA杂化前后方波伏安法的扫描... 详细信息

将二茂铁和巯基修饰的脱氧核糖核酸(DNA)探针通过自组装的方式固定到金电极表面,以二茂铁作为电子介体构建电化学DNA传感器,利用杂化前后DNA传感器所展现出峰电流的差异,实现对目标DNA的定量检测。通过研究DNA杂化前后方波伏安法的扫描频率与二茂铁电子介体传输速率关系,优化扫描频率。结果表明:当扫描频率200 Hz时,目标DNA浓度在5. 0×10^(-9)～1. 0×10^(-7)mol/L范围内,峰电流的变化与目标DNA浓度呈线性关系,线性拟合方程式为ΔI_P/I_0(%)=0. 760 95+0. 610 65 c,检测限为1. 7×10^(-9)mol/L(S/N=3)。

关键词：电化学脱氧核糖核酸(DNA)生物传感器二茂铁标记DNA 方波伏安法

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Evidence of Mutational Heterogeneity of the ALDH7A1 Gene in Tunisian Families： Molecular and Insilico Investigations

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材料科学与工程（中英文B版） 2016年第4期6卷 196-200页

作者： Abdelaziz tlili Marwa kharrat Emna Mkaouar-Rebai Nadia Hamida-Hentati Sawssan Ben Romdhan Nourhene Fendri-Kriaa Faiza Fakhfakh Laboratoire de Genetique Moleculaire Humaine Faculte de Medecine de Sfax University of Sfax 3084 Tunisia Department of Applied Biology College of Sciences University of Sharjah 27272 UAE Service de Neonatologie CHU Hedi Chaker Sfax 3084 Tunisia

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