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miR-155表达与FLT3-ITD^(+)急性髓系白血病细胞系药物敏感性的关系及机制研究

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中国实验血液学杂志 2024年第2期32卷 395-401页

作者：王凌燕江佩芳李佳蒸陈艳欣胡建达福建医科大学附属协和医院血液科福建省血液病学重点实验室福建省血液病研究所福建福州350001 福建医科大学福建福州350001 福建医科大学附属第二医院福建泉州362000 福建医科大学精准医学研究院福建福州350001

目的:研究miR-155的表达与FLT3-ITD^(+)急性髓系白血病细胞系药物敏感性的关系及潜在的调控机制。方法:通过CRISPR/Cas9基因编辑工具在FLT3-ITD^(+)AML细胞系MV411中实现miR-155编码基因敲除,筛选单克隆细胞,PCR及Sanger测序鉴定单克隆... 详细信息

目的:研究miR-155的表达与FLT3-ITD^(+)急性髓系白血病细胞系药物敏感性的关系及潜在的调控机制。方法:通过CRISPR/Cas9基因编辑工具在FLT3-ITD^(+)AML细胞系MV411中实现miR-155编码基因敲除,筛选单克隆细胞,PCR及Sanger测序鉴定单克隆细胞的基因型,实时荧光定量逆转录PCR鉴定成熟miRNA的表达水平。MTT法计算半数抑制率,比较MV411细胞对多柔比星、奎扎替尼以及米哚妥林的药物敏感性。转录组测序分析miR-155敲除后MV411细胞mRNA水平的变化,基因集富集分析获得miR-155敲除后的信号通路的变化水平,Western blot验证信号通路关键分子的表达。结果:通过PCR初筛和Sanger测序获得了4个基因敲除的杂合子和1个基因插入的杂合子。实时荧光定量逆转录PCR结果显示,这些单克隆细胞中成熟miR-155的表达显著低于野生型克隆。MTT结果显示,与野生型相比,miR-155基因敲除后MV411细胞对多种抗FLT3-ITD^(+)AML的药物敏感性有了显著增加。RNA测序显示miR-155敲除后mTOR信号通路和Wnt信号通路受到抑制。Western blot结果显示,信号通路关键分子p-mTOR、Wnt5α、β-catenin的表达下调。结论:miR-155敲除后能显著提升MV411细胞对多柔比星、奎扎替尼和米哚妥林的药物敏感性,这与包括mTOR及Wnt信号通路在内的多条信号通路的抑制有关。

关键词： miR-155 FLT3-ITD+急性髓系白血病多柔比星米哚妥林奎扎替尼

Application of the DEM to screening process:a 3D simulation

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Mining Science and Technology 2009年第4期19卷 493-497页

作者： chen yan-hua TONG xin School of Mechanical Engineering and Automation Huaqiao University Quanzhou Fujian 362021 China

The discrete element method(DEM) has been widely used to simulate microscopic interactions between *** is a deeply complicated process when considering the law of motion for the particles,*** this paper,a numerical model for the study of a particle screening process using the DEM is *** attention was paid to the modeling of a vibrating screen that allows particles to pass through,or to rebound,when approaching the screen *** concerning screen length and vibrating frequency as they relate to screening efficiency were *** conclusions were:three-dimensional simulation of screening efficiency along the screen length follows an exponential distribution;when the sieve vibrates over a certain frequency range the screening efficiency is stable;and,higher vibration frequencies can improve the handling capacity of the screening machine.

关键词： discrete element method screening efficiency digital simulation

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中国野生唇形科物种多样性及其地理格局分析

亚热带植物科学 2024年第3期53卷 234-242页

作者：常梦琳陈新艳陈世品马良福建农林大学风景园林与艺术学院福建福州350108 三明市园林中心福建三明365000 福建农林大学林学院福建福州350002 福建卫生职业技术学院福建福州350101

基于分类学数据库及大量文献资料,以行政县为基本单元对中国陆域分布的野生唇形科进行整理,统计其物种多样性、属的区系、分布地等信息,建立了中国野生唇形科植物地理分布数据集。截至2024年1月,共记录100属1387种(含种下单元),其中属... 详细信息

基于分类学数据库及大量文献资料,以行政县为基本单元对中国陆域分布的野生唇形科进行整理,统计其物种多样性、属的区系、分布地等信息,建立了中国野生唇形科植物地理分布数据集。截至2024年1月,共记录100属1387种(含种下单元),其中属内含种数>150的属仅有黄芩属Scutellaria;属内物种数介于101~150的属为鼠尾草属Salvia(137种)、香茶菜属Isodon(106种);属内物种数介于6~50的共有36属,合计733种,是中国野生唇形科植物的主要成分;属内包含种数≤5的属共有59个,合计119种,占中国野生唇形科植物总种数的8.58%;单种属多达33个,表明中国野生唇形科植物属内种较为贫乏但属多样性高的特点。以黄芩属、鼠尾草属、香茶菜属为主要分布属,3属共394种,占总物种数的28.62%;野生唇形科在我国所有省(自治区、直辖市)均有分布,主要分布在我国喜马拉雅山脉东段、鄂西渝东山地、藏东南–滇西北地区、滇东–桂西山地,尤以云南省(658种)、四川省(532种)物种丰富度为最高;属的区系成分多样,根据吴征镒对中国种子植物属的分布区类型的划分原则,中国野生唇形科植物100属归属于15个正型与12个变型,以热带区系及温带区系占主导,与热带区系分布有关的属共计37属479种,明显占优势地位;温带成分次之;也有6个地中海区系分布的属及2个东亚和北美洲间断分布的属渗入。中国野生唇形科植物功能多样,主要可分为药用植物类、香料植物类、蜜源植物类、野生蔬菜类、观赏植物类和油脂植物类。

关键词：植物区系唇形科生物多样性地理分布

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Overexpression of Sweet Pepper Glycerol-3-Phosphate Acyltransferase Gene Enhanced Thermotolerance of Photosynthetic Apparatus in Transgenic Tobacco

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Journal of Integrative Plant Biology 2008年第5期50卷 613-621页

作者： Kun yan Na chen yan-yan Qu xin-Chun Dong Qing-Wei Meng Shi-Jie Zhao College of Life Sciences State Key Laboratory of Crop Biology Shandong Agricultural University Tai'an 271018 China

In order to investigate the relationship between the lipid composition in thylakoid membrane and thermostability of photosynthetic apparatus, tobacco transformed with sweet pepper sense glycerol-3-phosphate acyltransferase （GPAT） gene were used to analyze the lipid composition in thylakoid membrane, the net photosynthetic rate and chlorophyll fluorescence parameters under high temperature stress. The results showed that the saturated extent of monogalactosyldiacylglycerol （MGDG）, sulfoquinovosyldiacylglycerol, digalactosyldiacylglycerol and phosphatidylglycerol in thylakoid membrane of transgenic tobacco T1 lines increased generally. Particularly, the saturated extent in MGDG increased obviously by 16.2% and 12.6% in T1-2 and T1-1, respectively. With stress temperature elevating, the maximum efficiency of photosystem Ⅱ （PSⅡ） photochemistry （Fv/Fm）, actual photochemical efficiency of PSll in the light （ФPSⅡ） and net photosynthetic rate （Pn） of the two lines and wild type tobacco plants decreased gradually, but those parameters decreased much less in transgenic plants. Even though the recovery process appeared differently in the donor and acceptor side of PSⅡ in transgenic tobacco compared with wild-type plants, the entire capability of PSⅡ recovered faster in transgenic tobacco, which was shown in the parameters of PI, Fv/Fm and ФPSⅡ, as a result, the recovery of Pn was accelerated. Conclusively, we proposed that the increase in saturated extent of thylakoid membrane lipids in transgenic plants enhanced the stability of photosynthetic apparatus under high temperature stress.

关键词： glycerol-3-phosphate transferase lipids in thylakoid membrane photosynthesis thermotolerance tobacco.

补肾祛瘀法对中重度宫腔粘连的抗纤维化作用及经血中细胞外基质降解相关因子的影响

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广州中医药大学学报 2024年第1期41卷 96-104页

作者：邢娉婷王爱丽陈彦辛海口市中医医院海南海口570000

【目的】探究补肾祛瘀法对中、重度宫腔粘连的抗纤维化作用及经血中细胞外基质降解相关因子的影响。【方法】将124例肾虚血瘀证中、重度宫腔粘连并拟行宫腔镜宫腔粘连分离术(TCRA)的患者随机分为研究组和对照组,每组各62例。2组患者均给... 详细信息

【目的】探究补肾祛瘀法对中、重度宫腔粘连的抗纤维化作用及经血中细胞外基质降解相关因子的影响。【方法】将124例肾虚血瘀证中、重度宫腔粘连并拟行宫腔镜宫腔粘连分离术(TCRA)的患者随机分为研究组和对照组,每组各62例。2组患者均给予TCRA治疗,对照组术后给予黄体酮+戊酸雌二醇片治疗,研究组在对照组的基础上联合补肾祛瘀方(以归肾汤加减)治疗,共治疗3个月经周期。观察2组患者治疗前后月经情况、阴道彩色多普勒超声检查指标、肾虚血瘀证评分和宫腔粘连评分的变化情况,检测2组患者治疗前后的血管内皮生长因子(VEGF)、转化生长因子β1(TGF-β1)、血小板源性生长因子(PDGF)、结缔组织生长因子(CTGF)以及基质金属蛋白酶9(MMP-9)m RNA、基质金属蛋白酶抑制因子1(TIMP-1)mRNA、磷脂酰肌醇-3-羟激酶(PI3K)m RNA、Ⅰ型胶原A1蛋白(COL1A1)mRNA的表达水平,并评估2组患者的临床疗效和安全性。【结果】(1)疗效方面:治疗3个月经周期后,研究组患者的总有效率为93.55%(58/62),对照组为80.65%(50/62),组间比较,研究组的疗效明显优于对照组(P0.05),且经对症处理后,2组患者的不良反应症状均消失,同时研究过程中未见与服用中药相关的不良反应。【结论】补肾祛瘀方联合西药治疗宫腔粘连TCRA术后患者较单用西药治疗效果更佳,加用补肾祛瘀方可进一步促进患者月经恢复,改善子宫内膜血流循环,增加子宫内膜厚度,调节患者体内纤维化因子表达,并可上调PI3K、MMP-9 mRNA表达,恢复患者细胞外基质(ECM)平衡,预防宫腔再粘连,且临床使用安全。

关键词：补肾祛瘀法归肾汤宫腔粘连肾虚血瘀纤维化因子细胞外基质子宫内膜

Bio-inspired Materials for Photocatalytic Hydrogen Production

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Chinese Journal of Structural Chemistry 2020年第12期39卷 2123-2130,2054页

作者： JIANG Xia chen yan-xin LU Can-Zhong CAS Key Laboratory of Design and Assembly of Functional Nanostructures and Fujian Provincial Key Laboratory of NanomaterialsFujian Institute of Research on the Structure of MatterChinese Academy of SciencesFuzhou 350002China Xiamen Key Laboratory of Rare Earth Photoelectric Functional Materials Xiamen Institute of Rare-earth MaterialsHaixi InstitutesChinese Academy of SciencesXiamen 361021China

After millions of years of evolution,species in nature have structures and complex elements that are difficult to synthesize ***,these fine structures and compositions are often beneficial to improve the photocatalytic ***,various materials with special morphology,pore structure and element composition derived from biomass have emerged and are widely *** mini review focuses on the preparation of bio-inspired materials and their current status in photocatalytic hydrogen ***,this will bring new perspectives to researchers and make them learn more about the advantages of"learning from nature"and pay more attention to the green design of material structures.

关键词： biomass bio-inspired materials photocatalytic hydrogen production

Balancing Exploration–Exploitation of Multi-verse Optimizer for Parameter Extraction on Photovoltaic Models

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Journal of Bionic Engineering 2024年第2期21卷 1022-1054页

作者： yan Han Weibin chen Ali Asghar Heidari Huiling chen xin Zhang College of Computer Science and Artificial Intelligence Wenzhou UniversityWenzhou325035China School of Surveying and Geospatial Engineering College of EngineeringUniversity of TehranTehranIran School of Biomedical Engineering National Engineering Research Center of Ophthalmology and OptometryEye HospitalWenzhou Medical UniversityWenzhou325027China Key Lab of Biohealth Materials and Chemistry of Wenzhou Wenzhou UniversityWenzhouChina Key Laboratory of Intelligent Informatics for Safety&Emergency of Zhejiang Province Wenzhou UniversityWenzhouChina

Extracting photovoltaic(PV)model parameters based on the measured voltage and current information is crucial in the simulation and management of PV *** accurately and reliably extract the unknown parameters of different PV models,this paper proposes an improved multi-verse optimizer that integrates an iterative chaos map and the Nelder–Mead simplex method,*** experiments verified that the proposed INMVO fueled by both mechanisms has more affluent populations and a more reasonable balance between exploration and ***,to verify the feasibility and competitiveness of the proposal,this paper employed INMVO to extract the unknown parameters on single-diode,double-diode,three-diode,and PV module four well-known PV models,and the high-performance techniques are selected for *** addition,the Wilcoxon signed-rank and Friedman tests were employed to test the experimental results *** evaluation metrics,such as root means square error,relative error,absolute error,and statistical test,demonstrate that the proposed INMVO works effectively and accurately to extract the unknown parameters on different PV models compared to other *** addition,the capability of INMVO to stably and accurately extract unknown parameters was also verified on three commercial PV modules under different irradiance and *** conclusion,the proposal in this paper can be implemented as an advanced and reliable tool for extracting the unknown parameters of different PV *** that the source code of INMVO is available at https://***/woniuzuioupao/INMVO.

关键词： Photovoltaic models Multi-verse optimizer Nelder-Mead simplex Iterative chaos map

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福建省紫草科一新记录属——皿果草属

陕西林业科技 2024年第2期52卷 98-100页

作者：马良陈新艳聂德旺胥喆陈世品福建卫生职业技术学院福州350101 三明市园林中心福建三明365000 邵武将石省级自然保护区福建邵武354000 福建省林业勘察设计院福州350001 福建农林大学林学院福州350002

报道福建省紫草科(BoraginaceaeJuss.)一新记录属——皿果草属(***),一新记录种——皿果草[***(Johnst.)***],凭证标本存放于福建农林大学林学院树木标本室(FJFC).

关键词：福建省皿果草属分布新记录属

Fine-grained grid computing model for Wi-Fi indoor localization in complex environments

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Journal of Electronic Science and Technology 2024年第1期22卷 42-52页

作者： yan Liang Song chen xin Dong Tu Liu School of Computer Engineering Chengdu Technological UniversityChengdu611730China HAN Networks Corporation Limited Beijing100094China

The fingerprinting-based approach using the wireless local area network(WLAN)is widely used for indoor ***,the construction of the fingerprint database is quite *** when the position of the access point(AP)or wall changes,updating the fingerprint database in real-time is *** appropriate indoor localization approach,which has a low implementation cost,excellent real-time performance,and high localization accuracy and fully considers complex indoor environment factors,is preferred in location-based services(LBSs)*** this paper,we proposed a fine-grained grid computing(FGGC)model to achieve decimeter-level localization *** points(RPs)are generated in the grid by the FGGC ***,the received signal strength(RSS)values at each RP are calculated with the attenuation factors,such as the frequency band,three-dimensional propagation distance,and walls in complex *** a result,the fingerprint database can be established automatically without manual measurement,and the efficiency and cost that the FGGC model takes for the fingerprint database are superior to previous *** proposed indoor localization approach,which estimates the position step by step from the approximate grid location to the fine-grained location,can achieve higher real-time performance and localization accuracy *** mean error of the proposed model is 0.36 m,far lower than that of previous ***,the proposed model is feasible to improve the efficiency and accuracy of Wi-Fi indoor *** also shows high-accuracy performance with a fast running speed even under a large-size *** results indicate that the proposed method can also be suitable for precise marketing,indoor navigation,and emergency rescue.

关键词： Fine-grained grid computing (FGGC) Indoor localization Path loss Random forest Reference points(RPs)