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Subcellular localization and functional analyses of structural domains of COP1 in transgenic tobacco

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chinese Science Bulletin 2002年第15期47卷 1250-1257,1321-1322页

作者： Wang, CX Huang, ch wu, GY wu, XY Zhao, JD Peking Univ Coll Life Sci Beijing 100871 Peoples R China

Plants have evolved an extremely exquisite light signal regulatory network to adapt to the changing ambient light conditions, in which COP1 plays a critical role of the light signal transduction. Based on the cloned pea COP1 cDNA sequence and its protein structure, four individual gene fragments encoding different structural domains of the COP1 were designed to fuse to the GFP gene. The plant expression vectors containing these fusion genes as well as the COPIGFP fusion gene were constructed and used to transform tobacco by Agribacterium as confirmed by South-]ern analyses. Antibodies were raised against the recombi-nant GFP-COP1 overproduced in Escherichia coli. 1m-munoblotting results demonstrated that all of the fusion genes were constitutively expressed in transgenic tobacco plants. We systematically investigated the different subcell-ular localization of these fusion proteins and the resulting phenotypic characteristics of these transgenic plants under light and dark conditions. Our data show that (

关键词： photomorphogenesis COP1 tobacco GFP subcellular localization.

Endoscopic hemoclip treatment for bleeding peptic ulcer

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World Journal of Gastroenterology 2000年第1期6卷 53-56页

作者： Lai YC Yang SS wu ch chen TK Division of Gastroenterology Cathay General HospitalTaipei Division of Gastroenterology Cathay General HospitalTaipei Medical FacultyChina Medical CollegeTaichungTaiwanChina

AIM To evaluate the efficacy of endoscopichemoclip in the treatment of bleeding *** Totally,40 patients with F1a andFib hemorrhagic activity of peptic ulcers wereenrolled in this uncontrolled prospective studyfor endoscopic hemoclip *** used anewly developed rotatable clip-device for theapplication of hemoclip(MD850)to *** was repeated if there wasany sign or suspicion of rebleeding,and re-clipping was performed if necessary *** Initial hemostatic rate by clippingwas 95%,and rebleeding rate was only 8%.Ultimate hemostatic rates were 87%,96%,and93% in the Fla and Flb subgroups,and totalcases,*** patients with shock onadmission,hemoclipping achieved ultimatehemostasis of 71% and 83% in F1a and F1bsubgroups,*** reached100% in patients without shock regardless ofhemorrhagic activity being F1a or *** number of clips used per case was 3.0(range 2-5).Spurting bleeders required moreclips on average than did oozing bleeders(3.4versus 2.8).We observed no obviouscomplications,no tissue injury,or impairmentof ulcer healing related to *** Endoscopic hemoclip placementis an effective and safe *** theimprovement of the clip and application device,the procedure has become easier and much *** hemoclipping deservesfurther study in the treatment of bleeding pepticulcers.

关键词： Subject headings endoscopic hemoclipping gastrointestinal hemorrhage/therapy peptic ulcer hemostasis

Spin order and dynamics in the topological rare-earth germanide semimetals

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Science china(Physics,Mechanics & Astronomy) 2024年第10期67卷 113-127页

作者： Yuhao Wang Zhixuan Zhen Jing Meng Igor Plokhikh Delong wu Dariusz J.Gawryluk Yang Xu Qingfeng Zhan Ming Shi Ekaterina Pomjakushina Toni Shiroka Tian Shang Key Laboratory of Polar Materials and Devices(MOE) School of Physics and Electronic ScienceEast China Normal UniversityShanghai 200241China Laboratory for Multiscale Materials and Experiments Paul Scherrer InstitutVilligen PSI CH-5232Switzerland Center for Correlated Matter and School of Physics Zhejiang UniversityHangzhou 310058China Laboratory for Muon-Spin Spectroscopy Paul Scherrer InstitutVilligen PSI CH-5232Switzerland Laboratorium für Festkörperphysik ETH ZürichZürich CH-8093Switzerland

The REAl(Si,Ge)(RE=rare earth)family,known to break both the inversion-and time-reversal symmetries,represents one of the most suitable platforms for investigating the interplay between correlated-electron phenomena and topologically nontrivial ***,we report on systematic magnetic,transport,and muon-spin rotation and relaxation(μSR)measurements on(Nd,Sm)AlGe single crystals,which exhibit antiferromagnetic(AFM)transitions at T_(N)=6.1 and 5.9 K,*** addition,NdAlGe undergoes also an incommensurate-to-commensurate ferrimagnetic transition at 4.5 *** transverse-fieldμSR measurements confirm the AFM transitions,featuring a~90%magnetic volume ***-field(ZF)μSR measurements reveal a more disordered internal field distribution in NdAlGe than in SmAlGe,reflected in a larger transverse muon-spin relaxation rateλTat Tch undergoes a series of metamagnetic transitions in an external magnetic field,while SmAlGe shows only a robust AFM *** NdAlGe,the topological Hall effect(THE)appears between the first and the second metamagnetic transitions for H‖c,while it is absent in *** THE in NdAlGe is most likely attributed to the field-induced topological spin *** longitudinal muon-spin relaxation rateλL,diverges near the AFM order,followed by a clear drop at T

关键词： magneticWeyl semimetals topological Hall effect spin fluctuations μSR

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煤体中瓦斯水合固化的力学作用研究进展

煤炭学报 2024年第2期49卷 720-738页

作者：吴强张保勇黑龙江科技大学安全工程学院黑龙江哈尔滨150022

为更好地完善我国煤与瓦斯突出预测与防治方法,基于煤与瓦斯突出综合作用假说,提出了瓦斯水合固化防治煤与瓦斯突出的方法。该方法核心是将煤层中瓦斯固化生成瓦斯水合物,不仅能降低瓦斯压力,而且能够提高煤体强度,以达到减弱或消除煤... 详细信息

为更好地完善我国煤与瓦斯突出预测与防治方法,基于煤与瓦斯突出综合作用假说,提出了瓦斯水合固化防治煤与瓦斯突出的方法。该方法核心是将煤层中瓦斯固化生成瓦斯水合物,不仅能降低瓦斯压力,而且能够提高煤体强度,以达到减弱或消除煤与瓦斯突出危险性的目的。实现了煤体中瓦斯水合物的生成以及含瓦斯水合物煤体力学性质-渗透率原位测试,提出了煤体中瓦斯水合物生成及力学性质-渗透率测试技术及含瓦斯水合物煤体三轴压缩数值建模技术。瓦斯水合固化热力学和动力学条件是防突的理论基础,煤层瓦斯水合物稳定储存是技术前提,瓦斯压力降低及煤体力学性质改善是防突关键,重点围绕水合固化煤体交叉力学问题进行总结。分析认为:(1)瓦斯水合固化防突技术理论框架已经初步形成,并利用数值模拟手段初步探究了瓦斯水合固化对煤体力学特征改善的细观机理;(2)现阶段已证实煤体中水合物生成不仅能降低瓦斯压力,而且能改善其力学性质,高饱和度对提高煤体峰值强度明显;(3)瓦斯水合物生成经历快速、缓慢和稳定3个阶段,且水合物的生成会造成煤体中瓦斯渗流通道阻塞,导致其渗透率降低;(4)高瓦斯压力、高ch4体积分数不仅有助于提高水合物饱和度而且会延缓水合物分解,有助于水合物的稳定存在。需要注意的是,瓦斯水合固化技术防突的可靠度仍需大量重复试验验证,以建立普适化的数据库。综合现有研究结果,对水合固化防突研究目前仍存在的局限性与挑战进行了讨论,并且给出了进一步的研究方向。

关键词：煤与瓦斯突出瓦斯水合固化含瓦斯水合物煤体力学性质离散元

Synergistic oxidation-reforming of biomass for high quality syngas production based on a bifunctional catalyst

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Green Carbon 2024年第1期2卷 118-123页

作者： Tao He Dan Zhang Wenqing chen Zeng Liu Ruidong Zhao Jianqing Li Jingli wu Zhiqi Wang Jinhu wu CAS Key Laboratory of Biofuels Qingdao Institute of Bioenergy and Bioprocess TechnologyChinese Academy of SciencesQingdao 266101China Shandong Energy Institute Qingdao 266101China Qingdao New Energy Shandong Laboratory Qingdao 266101China University of chinese Academy of Sciences Beijing 100049China

Conventional O_(2)gasification for low-rank biomass/sludge conversion is prone to high CO_(2)concentrations in the syngas because of its high O content and low calorific *** study establishes a synergistic oxidationreforming reaction route for the conversion of low-rank carbon-containing resources into high-quality *** efficient oxidation-reforming reaction is based on the bifunctional catalyst NiO-Fe_(2)O_(3)/Al_(2)O_(3),which includes Fe_(2)O_(3) oxidation sites and NiO reforming *** temperature-programmed reduction,together with X-ray diffraction and X-ray photoelectron spectroscopy experiments,demonstrated that the two functional active sites have strong interactions with the support,leading to efficient cooperation between the oxidation reaction and reforming reaction with regards to both the reaction sequence and C/H/O element *** produced from biomass/sludge based on oxidation-reforming reactions has an extremely low CO_(2)concentration of approximately 3%,and the valid gas(CO,H_(2))concentration exceeds 95%.The valid gas yield of walnut shell reached 1452.9 mL/g,the total gas yield was 1507.2 mL/g,and the H_(2)/CO ratio was 1.02,which are all very close to the theoretical maximum values of 1553.1 mL/g and 1.01,respectively,demonstrating that the inherent CO_(2)/H_(2)O along with ch4/tar species were efficiently converted to H_(2)and CO through oxidation-reforming *** a 60-cycle test,NiO-Fe_(2)O_(3)/Al_(2)O_(3) exhibited good redox stability.

关键词： Biomass Syngas Oxidation-reforming Bifunctional Catalyst

Regulated reaction pathway of CO_(2)photoreduction into ch_(4)by metal atom pair sites

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Rare Metals 2024年第9期43卷 4620-4627页

作者： Dong-Po He Guang-Bing Huang Jun Hu Yang wu Xiao-Dong Li Qing-Xia chen Shan Zhu Wen-Sheng Yan Jun-Fa Zhu Yang Pan Xing-chen Jiao Key Laboratory of Synthetic and Biological Colloids Ministry of EducationSchool of Chemical and Material EngineeringJiangnan UniversityWuxi 214122China Hefei National Research Center for Physical Sciences at Microscale National Synchrotron Radiation LaboratoryUniversity of Science and Technology of ChinaHefei 230026China Max Planck Institute of Microstructure Physics Weinberg 206120 HalleGermany State Grid Anhui Electric Power Research Institute Hefei 230601China

The carbon dioxide(CO_(2))reduction process involves complex protonation,making the resulting product often *** achieve the desired product,it is crucial to manipulate the reaction ***,we build the metal atom pair sites for selective CO_(2)photoreduction into *** a prototype,Ni atom pair sites loaded on the Mo S_(2)nanosheets were synthesized and verified by highresolution transmission electron microscopy(HRTEM),X-ray photoelectron spectroscopy(XPS)and X-rayabsorption near edge structure spectra(XANES).In-situ Fourier transform infrared spectroscopy(FTIR)monitors the*chO group,a crucial intermediate in ch_(4)production,during CO_(2)photoreduction on the Ni-Mo S_(2)nanosheets,whereas this monitoring is not observed for the Mo S_(2)***,theoretical calculations disclose that over the Ni-Mo S_(2)nanosheet slab,the formation energy of*chO intermediates is determined to be lower(0.585 e V)than the desorption energy of*CO intermediates for CO production(0.64 e V),implying the higher selectivity of ch_(4)production.

关键词： steps dioxide desired

1,3-氧硫戊环为受体的Hg^(2+)荧光探针的设计、合成及性能

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光谱学与光谱分析 2024年第7期44卷 1913-1917页

作者：梁庆祥周五吴爱斌舒文明余维初长江大学化学与环境工程学院化学系湖北荆州434023 长江大学非常规油气协同创新中心湖北荆州434023 油气田清洁生产与污染物控制湖北省工程研究中心湖北荆州434023

Hg^(2+)是毒性最强的重金属离子之一,会造成空气、土壤和水的污染,严重损害人体健康,开发有效的分析方法检测环境体系中的Hg^(2+)尤为重要。荧光探针因其灵敏度高、选择性好、响应速度快、可实时在线检测等优点,已广泛应用于Hg^(2+)检... 详细信息

Hg^(2+)是毒性最强的重金属离子之一,会造成空气、土壤和水的污染,严重损害人体健康,开发有效的分析方法检测环境体系中的Hg^(2+)尤为重要。荧光探针因其灵敏度高、选择性好、响应速度快、可实时在线检测等优点,已广泛应用于Hg^(2+)检测。以Hg^(2+)促进硫代缩醛的去保护反应设计合成了一种全新的以1,3-氧硫戊环为受体的开启型Hg^(2+)荧光探针[2-(pyren-1-yl)-1,3-oxathiolane,POX],通过^(1)H NMR、^(13)C NMR和HRMS对POX结构进行表征,考察了POX在ch_(3)ch_(2)OH/H_(2)O中对Hg^(2+)的选择性、竞争性、浓度滴定、pH滴定、时间依赖性、检出限和识别机理等。研究结果表明,POX可在较宽的pH范围内对Hg^(2+)快速识别,并表现出高度的选择性和灵敏度;向POX中加入Hg^(2+)后,在386 nm处出现明显的荧光发射峰,表明POX对Hg^(2+)呈现出显著的荧光“开启”效应,其识别过程几乎不受其他金属离子干扰;荧光滴定实验表明POX在Hg^(2+)浓度为0~6.5μmol·L^(-1)范围内具有良好的线性响应(R^(2)=0.9994),检出限为0.168μmol·L^(-1),在实际水样中检测Hg^(2+)的RSD小于2.92%。由于POX合成简单、原料易得且pH适用范围较广,可作为定性和定量检测环境中Hg^(2+)的潜在工具。

关键词：荧光探针 Hg^(2+) 1,3-氧硫戊环荧光开启

Synthesis,crystal structures,and antibacterial activities of two zinc(Ⅱ) complexes bearing 5⁃phenyl⁃1H⁃pyrazole group

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无机化学学报 2024年第10期40卷 1986-1994页

作者： LIU chao wu Jiang JIN Zhaolei School of chemistry and chemical Engineering Suzhou UniversitySuzhouAnhui 234000China Anhui Key Laboratory of Spin Electron and Nanomaterials SuzhouAnhui 234000China

A novel compound(H_(2)L)SCN(5⁃methyl⁃3⁃phenyl⁃2H⁃pyrazol⁃1⁃ium thiocyanate)has been obtained by the reaction of thiosemicarbazide with benzoylacetone in *** zinc􀃭complexes[Zn(HL)_(2)(NCS)(ch_(3)COO)](1)and[Zn_(2)(L)_(2)(HL)_(2)(NCS)_(2)]_(2)·2ch_(3)OH(2)have been synthesized by the coordination reactions of Zn(OAc)_(2)·2H_(2)O or ZnCl_(2)with(H_(2)L)SCN under reflux *** analyses and single⁃crystal X⁃ray diffraction have con⁃firmed the structures of the synthesized ***(H_(2)L)SCN ligand and complex 1 pertain to the triclinic sys⁃tem with space group P1,while complex 2 belongs to the monoclinic system with space group P2_(1)/***,the antibacterial activities of the compounds were evaluated in vitro using the agar diffusion method against the bac⁃terial strains(Candida albicans,Staphylococcus aureus,and Escherichia coli).The results showed that the ligand exhibited relatively good antibacterial activities against the bacteria,and the complexes possessed stronger antibac⁃terial activities against the same bacteria than the free ***:2190252,(H2L)SCN;2190253,1;2190256,2.

关键词： zinc􀃭complex 5⁃phenyl⁃1H⁃pyrazole thiocyanate crystal structure bacteria

High-rate ch_(4)-to-C_(2)H_(6) photoconversion enabled by Au/ZnO porous nanosheets under oxygen-free system

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Science china chemistry 2024年第3期67卷 869-875页

作者： Kai Zheng Xiaojing Zhang Jun Hu chengbin Xu Juncheng Zhu Jing Li Mingyu wu Shan Zhu Li Li Shumin Wang Yumei Lv Xin He Ming Zuo chengyuan Liu Yang Pan Junfa Zhu Yongfu Sun Yi Xie Hefei National Laboratory for Physical Sciences at Microscale National Synchrotron Radiation LaboratoryUniversity of Science and Technology of ChinaHefei 230026China Key Laboratory of Photochemical Conversion and Optoelectronic Materials Technical Institute of Physics and ChemistryChinese Academy of SciencesBeijing 100190China State Grid Anhui Electric Power Research Institute Hefei 230601China

Photocatalytic ch_(4) coupling into high-valued C_(2)H_(6) is highly attractive,whereas the photosynthetic rate,especially under oxygen-free system,is still ***,we designed the negatively charged metal supported on metal oxide nanosheets to activate the inert C-H bond in ch_(4)and hence accelerate ch_(4) coupling *** an example,the synthetic Au/ZnO porous nanosheets exhibit the C_(2)H_(6) photosynthetic rate of 1,121.6μmol g^(-1)_(cat)h^(-1)and the ch_(4) conversion rate of 2,374.6μmol g^(-1)_(cat)h^(-1) under oxygen-free system,2 orders of magnitude higher than those of previously reported *** virtue of several in situ spectroscopic techniques,it is established that the generated Au^(δ-)and O^-species together polarized the C-H bond,while the Au^(δ-)and O^-species jointly stabilized the ch_(3) intermediates,which favored the coupling of ch_(3) intermediate to photosynthesize C_(2)H_(6) instead of overoxidation into CO_(x).Thus,the design of dual active species is beneficial for achieving high-efficient ch_(4)-to-C_(2)H_(6) photoconversion.

关键词： dual surface-active sites selective methane coupling ethane room temperature