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Evaluating bioactivity and bioaccessibility properties of the propolis extract prepared with L-lactic acid: An alternative solvent to ethanol for propolis extraction

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FOOD BIOSCIENCE 2023年第3期53卷 2351-2358页

作者： Atayoglu, Ali Timucin Atik, Didem Sozeri Boluk, Esra Gurbuz, Basak ceylan, fatma duygu Capanoglu, Esra Atayolu, Rumeysa Paradkar, Anant Fearnley, James Palabiyik, Ibrahim Istanbul Medipol Univ Tradit & Complementary Med Dept Istanbul Turkiye Tekirdag Namik Kemal Univ Food Engn Dept Tekirdag Turkiye Istanbul Tech Univ Food Engn Dept Istanbul Turkiye Istanbul Medipol Univ Nutr & Dietet Dept Istanbul Turkiye Univ Bradford Ctr Pharmaceut Engn Sci Sch Pharm Bradford W Yorkshire England Apiceut Res Ctr Whitby N Yorkshire England

Ethanol readily solubilizes the active components of propolis. Due to certain disadvantages of ethanolic extracts, developing new non-ethanolic extraction methods is highly desirable. This study aimed to determine the content of phenolic compounds and antioxidant activity of propolis by using lactic acid extraction compared to ethanol. Various concentrations of ethanol and lactic acid were utilized to produce extracts, and an in vitro digestion procedure was applied to the extracts to compare the bioaccessibility of phenolic compounds by using LC-MS/MS in extracts and HPLC-DAD in digested samples. The compounds detected in the lactic acid-based propolis so-lution had similar values to those in the ethanol solution at a propolis concentration of 10%. The propolis-lactic acid solution had a significantly higher antioxidant activity against DPPH radical than the one prepared with ethanol at the same concentration (p < 0.05). Chrysin and naringenin were found as the most abundant phenolic compounds in the initial samples during in vitro digestion experiments. Pinocembrin was the most bioaccessible component among the dialyzed IN fractions of ethanol and lactic acid-based solutions, whereas some compounds were not detected. The results suggest that lactic acid may be used as an alternative to ethanol for propolis extraction.

关键词： Antioxidant Extraction Lactic acid Propolis Phenolics

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Comparison of visual and topographic outcomes of deep-anterior lamellar keratoplasty and penetrating keratoplasty in keratoconus

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International Journal of Ophthalmology(English edition) 2017年第3期10卷 385-390页

作者： Bora Yüksel Baran Kandemir Umut duygu Uzunel Ozan Celik Sezgin ceylan Tuncay Küsbeci Department of Ophthalmology Izmir Bozyaka Training and Research Hospital Izmir 35110 Turkey Department of Ophthalmology Kartal Training and Research Hospital Istanbul 34660 Turkey

AIM：To compare visual,surgical and topographic outcomes of deep anterior lamellar keratoplasty（DALK）and penetrating keratoplasty（PK）for keratoconus（KC）.METHODS：In this multicenter,prospective,randomized clinical trial 76 eyes of 71 KC patients operated between January 2011 and July 2014 in 2 tertiary referral hospitals were included. Consecutive patients were alternately selected to receive one of the two surgical methods. Thirty eight eyes underwent DALK with the big-bubble technique and 38 eyes underwent ***：Mean best spectacle corrected visual acuity（BSCVA）at the first postoperative week（P=0.012）and the first postoperative month（P〈0.001）was statistically significantly higher in DALK group. The mean BSCVA at12 mo was not significantly different for DALK（0.30±1.99 log MAR）versus PK（0.40±0.33 log MAR）（P=0.104）. The76.3% of the eyes had a BSCVA over 0.5 in DALK and 47.4%in PK group（P=0.009）. The 7.9% of the eyes had a BSCVA of 1.0 in DALK and 5.3% in PK group（P=0.644）. Mean spherical equivalent was-2.94 D in DALK and-3.09 D in PK *** topographic astigmatism was 4.62 D and 4.18 D respectively. Regular topographic patterns were observed in 31（81.6%）of DALK and 29（76.3%）of PK（P=0.574）. The most frequent topographic pattern was oblate asymmetric bow tie,seen in 39.5% in DALK and 23.7% in PK. CONCLUSION：Big bubble DALK provides an earlier visual improvement compare to PK. However,visual and topographic outcomes are similar to those in PK at 1y. Postoperative complications including rejection and intraocular pressure elevation are more frequent in PK. DALK is a safer alternative to PK for KC. However,intraoperativeperforation of the Descemet＇s membrane is a significant complication.

关键词： deep anterior lamellar keratoplasty keratoconus penetrating keratoplasty

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Surface Plasmon Resonance Based Sensor for Amaranth Detection With Molecularly Imprinted Nanoparticles

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Photonic Sensors 2023年第2期13卷 1-13页

作者： fatma Ozge OZGUR duyguÇIMEN Adil DENIZLI Nilay BERELI Department of Chemistry Hacettepe UniversityAnkara 06800Turkey

Amaranth imprinted nanoparticless were prepared by two-phase mini emulsion polymerization of hydroxyethyl methacrylate and ethylene glycol dimethacrylate using acrylamide and methacrylic acid as functional *** amaranth non-imprinted nanoparticle was prepared with the same procedure without using *** imprinted and non-imprinted nanoparticles were attached on the chip surface modified with allyl *** surfaces of the surface plasmon resonance(SPR)sensor were characterized by the ellipsometry,contact angle,and atomic force *** solutions with different concentrations(0.1 mg/mL-150mg/mL)were prepared with the pH 7.4 phosphate *** limit of detection and limit of quantification were 0.0180mg/mL and 0.06mg/mL,*** the selectivity of the amaranth imprinted SPR sensor was compared with the competing molecules tartrazine and allura red,it was observed that the target molecule amaranth was 5.64 times and 5.18 times more selective than allura red and tartrazine,*** liquid chromatography-mass spectrometry technique(LC-MS)was used for validation *** to the results obtained from both SPR sensor and LC-MS analyses,the amaranth recovery(%)from fruit juices was observed between 96%and 99%.

关键词： Amaranth surface plasmon resonance sensors fruit juice molecular imprinting

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Frequency and safety of COVID-19 vaccination in children with multisystem inflammatory syndrome:a telephonic interview-based analysis

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World Journal of Pediatrics 2022年第10期18卷 700-705页

作者： Kubra Aykac Kubra Ozturk Osman Oguz Demir Dilan Demir Gumus Sevgi Aslan Ela Cem Miray Yilmaz Celebi Mustafa Dogan Karabacak Gulsum Alkan fatma Dilsad Aksoy Burcu ceylan Cura Yayla Eda Kepenekli Solmaz Celebi Melike Emiroglu Ilker Devrim Ali Bulent Cengiz Mehmet Ceyhan Yasemin Ozsurekci Faculty of Medicine Department of Pediatric Infectious DiseasesHacettepe UniversityAnkaraTurkey Goztepe Prof.Dr.Suleyman Department of Pediatric RheumatologyYalcın City HospitalIstanbulTurkey Faculty of Medicine Department of Pediatric DiseasesHacettepe UniversityAnkaraTurkey Department of Pediatric Infectious Diseases Marmara University School of MedicineIstanbulTurkey Department of Pediatric Infectious Diseases Dr.Behçet Uz Children’s HospitalízmirTurkey Faculty of Medicine Department of Pediatric Infectious DiseasesSelcuk UniversityKonyaTurkey Faculty of Medicine Department of Pediatric Infectious DiseasesUludag UniversityBursaTurkey Department of Pediatric Infectious Diseases University of Health Science Ankara Training and Research HospitalAnkaraTurkey

Coronavirus disease 2019(COVID-19)vaccines are currently approved or authorized to prevent serious outcomes,such as severe disease,hospitalization,and *** Pfizer-BioNTech vaccine is recommended for everyone aged five and older in the United States(US)to prevent severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)infection[1,2].However,it is unclear whether children with a history of multisystem,inflammatory syndrome in children(MIS-C)may be at risk for a MIS-like response following COVID-19 vaccination or SARS-CoV-2 reinfection[2].Therefore,physicians remain uncertain about vaccinating children with a history of *** study investigates the occurrence of severe,systemic side effects of COVID-19 vaccines and the recurrence of the MIS-C symptoms in children with a history of MIS-C to overcome vaccine hesitancy.

关键词： vaccination vaccine Pfizer

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