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In vitro induced pluripotency from urine-derived cells in porcine

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World Journal of Stem Cells 2022年第3期14卷 231-244页

作者： Kaiana Recchia Lucas Simões Machado Ramon cesar Botigelli Naira Caroline Godoy Pieri Gabriela Barbosa Raquel Vasconcelos Guimarães de castro Mariana Groke Marques Laís Vicari de Figueiredo Pessôa Paulo Fantinato Neto Flávio Vieira Meirelles Aline Fernanda de Souza Simone Maria Massami Kitamura Martins Fabiana Fernandes Bressan Department of Surgery Faculty of Veterinary Medicine and Animal SciencesUniversity of São PauloPirassununga 13635-000São PauloBrazil Department of Pharmacology and Biotechnology Institute of BioscienceSão Paulo State UniversityBotucatu 18618-689São PauloBrazil Department of Veterinary Medicine Faculty of Animal Sciences and Food EngineeringUniversity of São PauloPirassununga 13635-000São PauloBrazil Embrapa Suínos e Aves Empresa Brasileira de Pesquisa AgropecuáriaConcordia 89715-899Santa CatarinaBrazil Department of Animal Sciences Faculty of Animal Sciences and Food EngineeringUniversity of São PauloPirassununga 13635-000São PauloBrazil

BACKGROUND The generation of induced pluripotent stem cells(iPSC)has been a game-changer in translational and regenerative medicine;however,their large-scale applicability is still hampered by the scarcity of accessible,safe,and reproducible *** porcine model is a large biomedical model that enables translational applications,including gene editing,long term in vivo and offspring analysis;therefore,suitable for both medicine and animal *** To reprogramme in vitro into pluripotency,and herein urine-derived cells(UDCs)were isolated from porcine *** The UDCs were reprogrammed in vitro using human or murine octamer-binding transcription factor 4(OCT4),SRY-box2(SOX2),Kruppel-like factor 4(KLF4),and C-MYC,and cultured with basic fibroblast growth factor(bFGF)*** characterize the putative porcine iPSCs three clonal lineages were submitted to immunocytochemistry for alkaline phosphatase(AP),OCT4,SOX2,NANOG,TRA181 and SSEA 1 *** transcripts related to the pluripotency(OCT4,SOX2 and NANOG)were analyzed via reverse transcription quantitative realtime polymerase chain reaction in different time points during the culture,and all three lineages formed embryoid bodies(EBs)when cultured in suspension without bFGF *** The UDCs were isolated from swine urine samples and when at passage 2 submitted to in vitro *** of putative iPSCs were obtained only from UDCs transduced with the murine factors(mOSKM),but not from human factors(hOSKM).Three clonal lineages were isolated and further cultured for at least 28 passages,all the lineages were positive for AP detection,the OCT4,SOX2,NANOG markers,albeit the immunocytochemical analysis also revealed heterogeneous phenotypic profiles among lineages and passages for NANOG and SSEA1,similar results were observed in the abundance of the endogenous transcripts related to pluripotent *** the clonal lineages when cultured in suspension without

关键词： Induced pluripotent stem cells Noninvasive Pluripotency Reprogramming Urine Porcine

Submegahertz spectral width photon pair source based on fused silica microspheres

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Photonics Research 2021年第11期9卷 2237-2252页

作者： ERASTO ORTIZ-RICARDO cesar BERTONI-OCAMPO MONICA MALDONADO-TERRON ARTURO GARCIA ZURITA ROBERTO RAMIREZ-ALARCON HECTOR CRUZ RAMIREZ R.castro-BELTRAN ALFRED B.U’REN Instituto de Ciencias Nucleares Universidad Nacional Autonoma de MexicoApartado Postal 70-54304510 MexicoD.F.Mexico Centro de Investigaciones en Optica A.C. Loma del Bosque 115Colonia Lomas del Campestre37150 LeonGuanajuatoMexico Departamento de Ingenieria Fisica Cuerpo Academico de Mecanica EstadisticaDivison de Ciencias e IngenieriasUniversidad de Guanajuato37150 LeonGuanajuatoMexico

High-efficiency submegahertz bandwidth photon pair generators will enable the field of quantum technology to transition from laboratory demonstrations to transformational applications involving information transfer from photons to *** spontaneous parametric processes are able to achieve high-efficiency photon pair generation,the spectral bandwidth tends to be relatively large,as defined by phase-matching *** solve this fundamental limitation,we use an ultrahigh quality factor(Q)fused silica microsphere resonant cavity to form a photon pair *** present the full theory for the spontaneous four-wave mixing(SFWM)process in these devices,fully taking into account all relevant source characteristics in our *** exceptionally narrow(down to kilohertz-scale)linewidths of these devices result in a reduction in the bandwidth of the photon pair generation,allowing submegahertz spectral bandwidth to be ***,using a pump source centered around 1550 nm,photon pairs with the signal and idler modes at wavelengths close to 1540 and 1560 nm,respectively,are *** herald a single idler-mode photon by detecting the corresponding signal photon,filtered via transmission through a wavelength division multiplexing channel of *** demonstrate the extraction of the spectral profile of a single peak in the single-photon frequency comb from a measurement of the signal–idler time of emission *** improvements in device design and experimental methods enabled the narrowest spectral width(Δν=366 k Hz)to date in a heralded single-photon source based on SFWM.

关键词： fused silica spectral

Ecological intensification of cropping systems enhances soil functions,mitigates soil erosion,and promotes crop resilience to dry spells in the Brazilian Cerrado

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International Soil and Water Conservation Research 2021年第4期9卷 591-604页

作者： Lucas de castro Moreira da Silva Junior cesar Avanzi Devison Souza Peixoto Marina Neves Merlo Emerson Borghi Alvaro Vilela de Resende Salvador Francisco Acuna-Guzman Bruno Montoani Silva Department of Soil Science Federal University of Lavras(UFLA)37200-900LavrasMGBrazil Department of Water Resources Federal University of Lavras(UFLA)37200-900LavrasMGBrazil Embrapa Maize and Sorghum Brazilian Agricultural Research Corporation(EMBRAPA)PO Box:28535701-970Sete LagoasMGBrazil Department of Physics Federal University of Lavras(UFLA)37200-900LavrasMGBrazil Center for Engineering and Industrial Development(CIDESI) Pie de la Cuesta 702Desarrollo San PabloQuerétaroQro.76125Mexico

Water scarcity threatens global food security and agricultural systems are challenged to achieve high yields while optimizing water *** deficit can be accentuated by soil physical degradation,which also triggers water losses through runoff and consequently soil *** soil health in cropping systems within the Brazilian Cerrado biome have been surveyed throughout the years,information about soil erosion impacts and its mitigation are still not well understood;especially concerning the role of cropping system diversification and its effects on crop ***,the aim of this study was to assess whether ecological intensification of cropping systems-inclusion of a consorted perennial grass and crop rotation-could promote soil coverage and consequently decrease water erosion and soil,water,and nutrient *** work studied the effects of crop rotation and consorted Brachiaria,along with different levels of investment in fertilization on soil physical quality and on soil,water,and nutrient losses,and crop *** proved that soybean monoculture(SS)is a system of low sustainability even under no-till in the Brazilian Cerrado *** exhibited high susceptibility to soil,water,and nutrient losses,causing low crop *** results showed that water losses in SS cropping system were approximately 10%of the total annual rainfall,and total K losses would require an additional 35%of K ***,ecological intensification of cropping systems resulted in enhanced soil environmental and agronomic functions,increased grain yield,and promoted soil and water conservation:high soil cover rate,and low soil,water and nutrient *** intensification proved to be an adequate practice to boost crop resilience to water deficit in the Brazilian Cerrado.

关键词： Crop rotation Grain production Nutrient losses Soil physical quality Tropical region

CC and NC Pion Production

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Journal of Modern Physics 2013年第12期4卷 1-5页

作者： Alejandro Mariano cesar Barbero Gabriel López-castro 1Departamento de F sica Facultad de Ciencias Exactas Universidad Nacional de La Plata Buenos Aires Argentina 2Instituto de Fsica La Plata CONICET Buenos Aires Argentina Departamento de F sica Cinvestav Mexico City Mexico

The disappearance searching experiments ?use charged current quasielastic (CCQE) reaction to detect an arriving neutrino and reconstruct its energy, while the CC1π+ production can mimic the CCQE signal process. In ?appearance experiments, the NC1π0 production process can lead to a fake e-?event by the impossibility for the detector of distinguish an arriving electron or a photon. Here we present a consistent model, from the point of view of the construction of the elemental amplitude, for the mentioned pion production background processes including bounding, smearing and final state interaction (FSI) effects in a single fashion. Results are comparable with more evolved approaches based on Monte Carlo simulations.

关键词： Neutrino Scattering Pion Production Neutrino Oscillation

Construction Processes Paradigm and Reinforcement Alternatives for Tailings Dam Soil

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Journal of Environmental Protection 2023年第3期14卷 213-229页

作者： Magno André Oliveira Lucas Redoan Cecílio Campos Marcela Cristina Costa Branco Junia Lúcio de castro Borges Gustavo Filemon Costa Lima Alan Rodrigues Teixeira Machado Augusto cesar da Silva Bezerra School of Mines Geotechnics Nucleus Federal University of Ouro Preto Ouro Preto Brazil Institute of Geosciences Federal University of Pará Belém Brazil Transport Engineering Department Federal Center for Technological Education of Minas Gerais Belo Horizonte Brazil Department of Geography Federal University of Minas Gerais Belo Horizonte Brazil Department of Exact Sciences University of the State of Minas Gerais João Monlevade Brazil

In the last decade, Brazil was the scene of two of the world’s biggest disasters related to mining tailings dams. The collapse of the Mariana and Brumadinho dams was catastrophic event that brought to light major environmental and humanitarian crises. Facts like this incite debate on the safety of dam construction methods and highlight the need for a broad discussion of dam safety legislation in the country. From the above, this paper promotes a review of various forms of construction of tailings dams and their associated problems, as well as discusses the techniques for soil reinforcement and their construction methods. The work also brings an overview of the Brazilian dams from April to July 2019, tracing the context of the Brazilian tailings dams months after the tragedy of Brumadinho. The methodology includes a robust literature review and discourse analysis. In Brazil, the number of ruptured dams is alarming, especially in the state of Minas Gerais, in which more than 7 have broken in the last two decades. Among the damages, the deaths impact society abruptly and were cataloged 455 deaths, of which 216 correspond to the rupture of the dam of the Córrego do Feijão mine in Brumadinho. To evaluate and diagnose a dam, one should consider ordinance N ° 70,389, 2017 revoked by Resolution ANM n ° 95, of February 7, 2022. In this context, in March 2019, we experienced several disturbing situations regarding dam instability in the state of Minas Gerais, being categorized as risky structures. To exemplify, one can cite dams classified as level 2 risk that became level 3. Due to the above facts, several studies are being conducted to improve soil aspects. For example, we highlight the increase in soil resistance that can be done using commercial and alternative materials available in the environment.

关键词： Dams Reinforcement Rupture Minas Gerais

Ursolic Acid Derivatives Induced Apoptosis and Reduces the NF-κB in Human Lung Adenocarcinoma Cells

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Journal of Cancer Therapy 2019年第10期10卷 863-876页

作者： Elaine Carlos Scherrer Ydia Mariele Valadares Caio cesar Souza Alves Gabriella Freitas Ferreira Marcela Pereira Leao Jessica Aline Soares Fernando Sa Silva Alessandra Paula Carli Oswaldo Cardoso Jr. Fabiana Simao Machado Sandra Bertelli Ribeiro castro Life Sciences Institute Federal University of Juiz de ForaGovernador ValadaresMGBrazil Faculty of Medicine Federal University of the Valleys of Jequitinhonha and MucuriTeofilo OtoniMGBrazil Department of Biochemistry and Immunology Federal University of Minas GeraisBelo HorizonteMGBrazil

Lung cancer is the major cause of death in the neoplastic diseases. In spite of the advances in the chemotherapy, the lung cancer treatments are still complex and costly, being necessary the seeking of new drugs. In this context, the ursolic acid (UA) becomes the target of studies that investigate its antitumor potential and, thus, structural modifications can enhance its biological activities. Eight UA semisynthetic derivative compounds (UAD1-8) were synthesized and evaluated their cytotoxic activity against human alveolar adenocarcinoma cells (A549). UAD1, UAD3, UAD5, UAD6 and UAD8 were able to reduce the viability of the A549 cells. Only UAD1 and UAD6 reduced the viability at 24 h, and only UAD3 didn’t reduce the NF-κB expression. The compound UAD1 showed the greater apoptosis induction. Moreover, the compound UAD1 showed better results than UA in all assays. The present study shows, for the first time, the action of these compounds in the apoptotic effect, in the expression of NF-κB and in the A549 cell line. The ursolic acid derivatives showed substantial results in the apoptosis, cytotoxicity and NF-κB inhibition of A549 cells, and further studies are necessary for the development of possible new therapeutic drugs.

关键词： Ursolic Acid Apoptosis NF-κB A549 Cells Tumoral