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Micro-plate magnetic chemiluminescence immunoassay and its applications in carcinoembryonic antigen analysis

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Science China Chemistry 2010年第4期53卷 812-819页

作者： LI ZhiYong1, ZHANG QianYun2. ZHAO LiXia1, LI ZhenJia3. HU GuoMao3. LIN Jin-Ming2.& WANG Shan4 1{2. Key Laboratory of Environmental Chemistry and Ecotoxicology, Research center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China 2.epartment of Chemistry, Tsinghua {2., Beijing 100084, China 3.eijing Chemclin Biotech Co., Ltd., Beijing Academy of Science and Technology, Beijing 100094, China 4School of Chemistry and Chemical Engineering, Xianyang Normal College, Xianyang 712.00, China 1. state Key Laboratory of Environmental Chemistry and Ecotoxicology Research Center for Eco-Environmental Sciences Chinese Academy of Sciences Beijing 100085 China2. Department of Chemistry Tsinghua University Beijing 100084 China3. Beijing Chemclin Biotech Co. Ltd. Beijing Academy of Science and Technology Beijing 100094 China4. School of Chemistry and Chemical Engineering of Xianyang Normal College Xianyang 712000 China

A micro-plate magnetic chemiluminescence immunoassay was developed for rapid and high throughput detection of carcinoembryonic antigens (CEA) in human sera. This method was based on a sandwich immunoreaction of fluorescein isothiocyanate (FITC)-labeled anti-CEA antibodies, CEA antigens, and horseradish peroxidase (HRP)-conjugated anti-CEA antibodies in mi- cro-plate. The immunomagnetic particles coated with anti-FITC antibodies were used as the solid phase for the immunoassay. The separation procedure was carried out by a magnetic plate adaptor and the luminol-hydrogen peroxide (H2.2.-HRP system was employed for the chemiluminescence detection. The proposed method combined the advantages of the micro-plate reactor and magnetic particle separation technology with the linear range of 5-2.0 ng mL·1. The detection limit of CEA was 0.61 ng mL·1. The coefficient of the variation was less than 7% and 13. for intra-assay and inter-assay precision, respectively. Compared with the commercial micro-plate chemiluminescent kit, the proposed method showed a good correlation.

关键词： micro-plate magnetic chemiluminescence immunoassay carcinoembryonic antigen tumor marker

Synthesis and characterization of a three-ring bent-core compound

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Science Bulletin 2005年第17期 35-39页

作者： TANG Yanhong1, MEI Chongyu1, LI Shaojun2. WANG Yan1, WANG Xingdong1, WANG Lixiang1, YAN Donghang1 & YE Cheng2.1. state Key Laboratory of Polymer Physics and Chemistry, changchun Institute of Applied Chemistry, Chinese Academy of Sciences,{2., China 2. {3. for Molecular Science, Laboratory of Organic solid, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100080, China

To investigate the vacuum-deposited films of the banana-shaped mesogens, we prepared a three-ring bent-core (that is, banana-shaped) compound m-bis(4-p- octoxysryrenyl)benzene (m-OSB) and used it as a prototype for studying the related film physics. Herein the synthesis and the structural characterization of m-OSB are reported. 13. and 1H NMR spectra and IR spectrum confirm that m-OSB has a symmetric and all-trans conformation. The results of differential scanning calorimetry, polarizing optical microscopy and X-ray diffractometry indicate that no liquid crystalline phase but two crystalline phases are present in this material: phase I (T<66℃) and phase II (66℃更多还原AbstractFilter('ChDivSummary', 'ChDivSummaryMore', 'ChDivSummaryReset');

关键词： banana-shaped mesogens bent-core molecules anti-ferro-electric alignment.

Grouping of amino acids and recognition of protein structurally conserved regions by reduced alphabets of amino acids

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Science China(Life Sciences) 2007年第3期50卷 392-402页

作者： LI Jing1 & WANG Wei1,2.1 National Laboratory of solid state Microstructure and Department of Physics, Nanjing {2., Nanjing 2.0093. China 2.Interdisciplinary {3. of Theoretical Studies, Chinese Academy of Sciences, Beijing 100080, China 1. National Laboratory of solid state Microstructure and Department of Physics Nanjing University Nanjing 210093 China 2. Interdisciplinary center of Theoretical Studies Chinese Academy of Sciences Beijing 100080 China

Sequence alignment is a common method for finding protein structurally conserved/similar regions. However, sequence alignment is often not accurate if sequence identities between to-be-aligned se- quences are less than 3.%. This is because that for these sequences, different residues may play similar structural roles and they are incorrectly aligned during the sequence alignment using substitu- tion matrix consisting of 2. types of residues. Based on the similarity of physicochemical features, residues can be clustered into a few groups. Using such simplified alphabets, the complexity of protein sequences is reduced and at the same time the key information encoded in the sequences remains. As a result, the accuracy of sequence alignment might be improved if the residues are properly clustered. Here, by using a database of aligned protein structures (DAPS), a new clustering method based on the substitution scores is proposed for the grouping of residues, and substitution matrices of residues at different levels of simplification are constructed. The validity of the reduced alphabets is confirmed by relative entropy analysis. The reduced alphabets are applied to recognition of protein structurally conserved/similar regions by sequence alignment. The results indicate that the accuracy or efficiency of sequence alignment can be improved with the optimal reduced alphabet with N around 9.

关键词： Grouping of amino acids and recognition of protein structurally conserved regions by reduced alphabets of amino acids

自主与不自足经济社会情境中的经济法定位:“社会性”的解读

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法制与社会发展 2005年第3期11卷 64-72页

作者：刘红臻肖乾刚吉林大学理论法学研究中心吉林长春130012 郑州大学法学院河南郑州450001

经济法被界定为“社会法”,对于“社会性”的解读构成理性认知经济法内在规定性、明智设计经济法制度、进而合理型塑经济社会生活的纽结。由于“社会性”实现机制的构设实为个体、社会和国家间制衡格局的构建,自由主义和福利国家论两大... 详细信息

经济法被界定为“社会法”,对于“社会性”的解读构成理性认知经济法内在规定性、明智设计经济法制度、进而合理型塑经济社会生活的纽结。由于“社会性”实现机制的构设实为个体、社会和国家间制衡格局的构建,自由主义和福利国家论两大理论范式对经济法产生了共识和分歧。在上述两种理论范式的智识洞见和理论缺陷的基础上,商谈论范式的相关立场和主张将有助于对经济法“社会性”定位的理论重解和制度重构。

关键词：社会性社会情境解读法定自足自主经济社会生活理论范式内在规定性理性认知经济法制实现机制福利国家自由主义理论缺陷制度重构社会法明智

Research on Aerodynamic Characteristics of Steel Wheel

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Research on Aerodynamic Characteristics of Steel Wheel

2.10 Second International Conference on Computational Intelligence and Natural Computing (CINC 2.10)

作者： Guohua Wang Qing Gao state Key Laboratory of Automobile Dynamic Simulation jilin {2. {3. 13.02. China Yan Liu Zhehao Xuan College of Automotive Engineering jilin {2. {3. 13.02. China

For the aerodynamic drag characteristics of the steel wheel using the rim which had 12.holes on it, aerodynamic simulation model was constructed to analyze the problem of aerodynamic drag characteristics. The changes of surface static pressure and the Characteristics of flow field were analyzed by simulation model. Analysis of the causes of aerodynamic drag, and supplied theory suggests for aerodynamic drag reduction of the steel wheel.

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税收质量的界定、制度比较与借鉴

学习与探索 2005年第1期 178-183页

作者：邵学峰吉林大学中国国有经济研究中心吉林长春130012

税收质量是指税收效率、税收公平及两者均衡的状态,主要体现在税收结构、税收分权体制以及税收征收管理机制三个方面上。不同经济发展水平国家的税收质量存在着较大差异性。税收质量要注重长远利益与现实利益的兼顾,在利益导向和控制导... 详细信息

税收质量是指税收效率、税收公平及两者均衡的状态,主要体现在税收结构、税收分权体制以及税收征收管理机制三个方面上。不同经济发展水平国家的税收质量存在着较大差异性。税收质量要注重长远利益与现实利益的兼顾,在利益导向和控制导向基础上平衡各级政府的税权划分,不断强化以税收服务和监督保障为核心的税收征收管理运行机制。从短期看,我国税收质量仍旧要以税收效率作为主要目标,兼顾税收公平作用的发挥;随着生产力水平的不断提高,要逐步强化税收公平,以税收公平促进税收效率。

关键词：税收质量制度研究税收效率税收公平

Application of Element-Free Method to water quality analysis of Miyun reservoir

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Science China earth Sciences 2005年第Z2期48卷 303-310页

作者： GU Zhaosheng, YANG Tianxing & XU Qing changchun Institute of Technology, changchun 1{2.012. China College of Geo-Exploration Science and Technology,{2.,{{2., China National Research center of Geoanalysis, Beijing 1000{2., China changchun Institute of Technology Changchun 130012 China College of Geo-Exploration Science and Technology Jilin University Changchun 130026 China College of Geo-Exploration Science and Technology Jilin University Changchun 130026 China National Research center of Geoanalysis Beijing 100037 China

This paper presents a 3. mathematical model for transfer and transform of water contaminants in reservoir, describes the discrete methods and the compute process of using an element-free collocation method. By these models and methods, the water quality conditions of Miyun reservoir area, the quantified description of three dimensions concerning the distribution and change of diversified contaminants are obtained within the assigned time. The distributions of total nitrogen and total phosphorus of the reservoir area in 2.03. 2.04 and 2.05 are simulated and forecasted by the boundary conditions in 2.03. so the main development trend is shown. It is found through calculation that: The contribution of the bed mud with regard to total phosphorus is comparatively marked. On the whole, the influence factor of the bed mud in the reservoir area and other comprehensive factors to total phosphorus are quite great. The influence of the living things or other function in the surface water of the Chaohe River valley in the east of the reservoir on the total phosphorus is comparatively conspicuous. Within the reservoir area in spring, summer and autumn, the concentration distribution of total nitrogen is basically in the trend of a slow progressive decrease, but the concentration of total phosphorus increases progressively. It is just contrary in autumn, winter and spring, the concentration of total nitrogen increases and the concentration of total phosphorus decreases. On the whole of the year's development, the total nitrogen is basically in a steady state, and the total phosphorus is in the increasing trend with years, but the kind of the trend is not conspicuous.

关键词： Miyun reservoir, water wuality analysis, Element-Free Method.

Influence factors and mechanism of emission of ZnS:Cu nanocrystals

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Chinese Physics B 2015年第6期24卷 586-590页

作者：楚学影王欣浓李金华姚丹方铉方芳魏志鹏王晓华 School of Science International Joint Research Center for Nanophotonics and BiophotonicsChangchun University of Science and Technology state Key Laboratory of High Power Semiconductor Lasers Changchun University of Science and Technology Nanchang university

Copper-doped ZnS （ZnS：Cu） nanocrystals are synthesized by the sol-gel method. The average size of the ZnS：Cu nanocrystals is 3.1 nm. The x-ray diffraction indicates that increasing the Cu-dopant concentration results in a large shift in the diffraction angle. The effects of the dopant concentration, the reactant ratio, and aging temperature on the optical properties of the ZnS：Cu nanocrystals are also investigated. The fluorescence emission mechanism is analyzed by peak deconvolution using Gaussian functions. We find that the emission of the ZnS：Cu nanocrystal is composed of different luminescence centers at 43., 470, 490, 52., and 560 nm. The origins of these emissions are discussed and demonstrated by controlled experiments.

关键词： ZnS:Cu luminescence Gaussian fitting

Simulation of nuclei morphologies for binary alloy

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Science China Mathematics 2010年第11期53卷 2927-2936页

作者： LI DaMing1, & ZHANG PingWen2.1Department of Mathematics, Shanghai Jiaotong university, Shanghai 2.02.0, China 2.ey Lab of Mathematics and Applied Mathematics, center for Applied Physics and Technology and School of Mathematical Sciences, Peking university, Beijing 100871, China 1. Department of Mathematics Shanghai Jiaotong University Shanghai 200240 China2. Key Lab of Mathematics and Applied Mathematics Center for Applied Physics and Technology and School of Mathematical Sciences Peking University Beijing 100871 China

We study the critical nuclei morphologies of a binary alloy by the string method. The dynamic equation of the string, connecting the metastable phase (liquid) and stable phase (solid), is governed by Helmholtz free energy for the binary alloy system at a given temperature. The stationary string through the critical nucleus (saddle point) is obtained if the relaxation time of the string is su?ciently large. The critical nucleus radius and energy barrier to nucleation of a pure alloy with isotropic interface energy in two and three dimensions are calculated, which are consistent with the classical nucleation theory. The critical nuclei morphologies are sensitive to the anisotropy strength of interface energy and interface thickness of alloy in two and three dimensions. The critical nucleus and energy barrier to nucleation become smaller if the anisotropy strength of the interface energy is increased, which means that it is much easier to form a stable nucleus if the anisotropy of the interface energy is considered.

关键词： nucleation crystal morphology string method