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急性心肌梗死患者黏附分子及相关因素的研究

中国危重病急救医学 2001年第10期13卷 615-617页

作者：姜霞田凤石张秀泉赵明雒容杨军张宏王金良天津市公安医院天津300050

目的 :探讨急性心肌梗死 ( AMI)患者发病早期至 1周内血清可溶性细胞间黏附分子 1 ( s ICAM1 )、可溶性血管细胞黏附分子 1 ( s VCAM 1 )、D 二聚体 ( D D)、血小板第 4因子 ( PF4 )的动态变化及其相互关系。方法 :测定 4 0例 AMI患者发... 详细信息

目的 :探讨急性心肌梗死 ( AMI)患者发病早期至 1周内血清可溶性细胞间黏附分子 1 ( s ICAM1 )、可溶性血管细胞黏附分子 1 ( s VCAM 1 )、D 二聚体 ( D D)、血小板第 4因子 ( PF4 )的动态变化及其相互关系。方法 :测定 4 0例 AMI患者发病 2 4小时和 1周时的血清 s ICAM 1、s VCAM 1、D D、PF4并与健康对照组 3 0例比较。结果 :AMI患者于发病 2 4小时及 1周时 s ICAM 1、s VCAM 1、D D、PF4〔发病 2 4小时分别( 42 0 .97± 1 2 8.83 )μg/L ,( 51 .4 4± 1 4.83 )μg/L ,( 3 57.93± 1 47.0 9)μg/L和 ( 6.1 0± 1 .54 ) mg/L ;发病 1周时分别为 ( 40 4 .96± 1 1 5.2 2 ) μg/L,( 48.0 1± 1 7.2 4 ) μg/L,( 3 81 .1 2± 1 63 .0 0 ) μg/L和 ( 6.3 3± 2 .1 9) mg/L〕均明显高于对照组〔分别为 ( 1 80 .70± 3 2 .0 7)μg/L,( 1 3 .3 1± 1 2 .81 )μg/L ,( 84 .0 0± 2 6.99)μg/L和 ( 3 .0 7± 1 .3 4) mg/L ,P均 0 .0 5)。结论 :AMI从发病早期至 1周内 s ICAM 1、s VCAM

关键词：心肌梗死急性细胞间黏附分子血管细胞黏附分子 D-二聚血小板第4因子

丙型肝炎病毒多表位基因核酸疫苗的构建及其免疫原性

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中国生物制品学杂志 2007年第9期20卷 633-636页

作者：韦三华尹文雷迎峰胡兴斌董轲李斌张青张惠中第四军医大学唐都医院临床实验与检验输血科西安710038 第四军医大学基础部微生物学教研室西安710032

目的构建丙型肝炎病毒（HCV）多表位基因的真核表达载体pcDNA3.1（-）-CtEm,用该重组质粒免疫BALB/c小鼠,并检测其免疫原性.方法用BamHⅠ和Hind Ⅲ双酶切含有HCV C区、E2区模拟表位和NS3～NS5区细胞表位串联基因的原核表达载体pQE30-C... 详细信息

目的构建丙型肝炎病毒（HCV）多表位基因的真核表达载体pcDNA3.1（-）-CtEm,用该重组质粒免疫BALB/c小鼠,并检测其免疫原性.方法用BamHⅠ和Hind Ⅲ双酶切含有HCV C区、E2区模拟表位和NS3～NS5区细胞表位串联基因的原核表达载体pQE30-CtEm,克隆入真核表达载体pcDNA3.1（-）,脂质体瞬时转染CHO细胞,并检测其表达.以100 μg重组质粒免疫BALB/c小鼠,检测其体液免疫和细胞免疫效果.结果所构建的真核表达载体pcDNA3.1（-）-CtEm在CHO细胞中能获得有效表达.该质粒免疫小鼠后可诱导高水平的抗体,特异性淋巴细胞增殖反应、IFN-γ水平及CTL活性均明显高于空载体和生理盐水对照组.结论已成功构建HCV多表位基因的真核表达载体pcDNA3.1（-）-CtEm,免疫小鼠后可诱导特异性体液免疫和细胞免疫应答.

关键词：丙型肝炎病毒多表位基因核酸疫苗免疫原性

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糖尿病足介入综合诊治临床指南(第五版)

介入放射学杂志 2019年第3期28卷 205-216页

作者：中国糖尿病足细胞与介入治疗技术联盟,中国介入医师分会介入医学与生物工程技术委员会李茂全不详同济大学附属第十人民医院介入血管外科同济大学医学院介入血管研究院上海200072

糖尿病足是糖尿病最常见的并发症之一,具有发病率高、致残率高、致死率高和治愈率低的特点,严重影响国人身体健康和生活质量,对其却一直没有统一的规范治疗方法。2015年,中国糖尿病足细胞与介入治疗技术联盟制定糖尿病足介入综合诊治临... 详细信息

糖尿病足是糖尿病最常见的并发症之一,具有发病率高、致残率高、致死率高和治愈率低的特点,严重影响国人身体健康和生活质量,对其却一直没有统一的规范治疗方法。2015年,中国糖尿病足细胞与介入治疗技术联盟制定糖尿病足介入综合诊治临床指南(第一版),填补了国内糖尿病足治疗规范的空白,对提高我国救治水平起到重要作用。近10年来,随着诊断标准和治疗方式更新及国内外相关研究不断深入,联盟组织国内89家单位在第四版基础上进行新的修订。现颁布第五版指南,以进一步规范我国糖尿病足临床诊治。

关键词：糖尿病足/糖尿病介入综合诊治临床指南

DEVELOPment OF NEEDLE-BASED MICrOENDOSCOPY FOr FLUOrESCENCE MOLECULAr IMAGING OF BrEAST TUMOr MODELS

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Journal of Innovative Optical Health Sciences 2009年第4期2卷 343-352页

作者： CHAO-WEI CHEN TIFFANY r.BLACKWELL rENEE NAPHAS PAUL T.WINNArD Jr VENU rAMAN KrISTINE GLUNDE YU CHEN Fischell depar.ment of Bioengineering and Electrical and Computer Engineering University of Maryland College ParkMD 20742USA In Vivo cellular and Molecular Imaging Center(ICMIC)depar.ment of radiology Johns Hopkins University School of Medicine BaltimoreMD 21205USA§yuchen@umd.edu¶kglunde@mri.jhu.edu

Fluorescence molecular imaging enables the visualization of basic molecular processes such as gene expression,enzyme activity,and disease-specific molecular interactions in vivo using targeted contrast agents,and therefore,is being developed for early detection and in situ characterization of breast *** advances in developing near-infrared fluorescent imaging contrast agents have enabled the specific labeling of human breast cancer cells in mouse model *** synergy with contrast agent development,this paper describes a needle-based fluorescence molecular imaging device that has the strong potential to be translated into clinical breast biopsy *** microendoscopy probe is based on a gradient-index(GrIN)lens interfaced with a laser scanning *** of the imaging performance,including the field-of-view,transverse resolution,and focus tracking characteristics were *** MDA-MB-231 breast cancer xenografts stably expressing the tdTomato red fluorescent protein(rFP)were used to detect the tumor cells in this tumor model as a proof of principle *** further development,this technology,in conjunction with the development of clinically applicable,injectable fluorescent molecular imaging agents,promises to perform fluorescence molecular imaging of breast cancers in vivo for breast biopsy guidance.

关键词： Breast cancer fluorescence molecular imaging microendoscopy optical imaging tdTomato fluorescent protein.

Overexpression of HSP27 and HSP70 is associated with decreased survival among patients with esophageal adenocarcinoma

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World Journal of clinical Cases 2019年第3期7卷 260-269页

作者： Henna K S?derstr?m Juha T Kauppi Niku Oksala Timo Paavonen Leena Krogerus Jari r?s?nen Tuomo rantanen depar.ment of General Thoracic Surgery Helsinki University Central Hospital Faculty of Medicine and Life Sciences University of Tampere depar.ment of Vascular Surgery Tampere University Hospital depar.ment of Pathology Fimlab Laboratories and Department of Medicine and Life Sciences University of Tampere depar.ment of Pathology Helsinki University Central Hospital depar.ment of Surgery Kuopio University Hospital depar.ment of Surgery Institute of Clinical Medicine University of Eastern Finland

BACKGrOUND Overexpression of heat shock proteins(HSPs) is associated with several malignancies and contributes to the development, progression, and metastasis of cancer, in addition to the inhibition of cellular death. In recent years, there has been active research into using HSP inhibitors in several malignancies. Due to the poor prognosis of esophageal adenocarcinoma(EAC), it would be valuable to find new biomarkers for the development of cancer *** To evaluate the expressions of HSP27 and HSP70 and their effect on survival in *** Immunohistochemical analyses and evaluations of HSP27 and HSP70 expression were performed on all available samples from 93 patients diagnosed with EACbetween 1990 and 2007 at two univer.ity hospitals. Fifteen cases with Barrett's metaplasia and 5 control cases from the same patient population were included in the analysis. HSP expression was quantitatively assessed and classified as high or low. Kaplan-Meier analyses and Cox regression models adjusting for age and sex as well as tumor site, stage, and grade were used to evaluate the effect on *** Tumor stage and surgical treatment were the main prognostic factors. High HSP27 expression in cancer cases was a strong negative predictive factor, with a mean survival of 23 mo compared to the 49 mo in cases with a low expression(P= 0.018). The results were similar for HSP70, with a poorer survival of 17 mo in cases with high HSP70 expression, in contrast to 40 mo(P = 0.006) in cases with a low expression. A Cox regression survival analysis was performed, adjusting for possible confounding factors, and higher HSP27 and HSP70 expressions remained an independent negative prognostic factor. The HSPs' correlation with survival was not affected by cancer treatments. When the analysis was adjusted for all factors, the odds ratios for HSP27 and HSP70 were 3.3(CI: 1.6–6.6, P =0.001) and 2.2(CI: 1.2–3.9, P = 0.02), *** HSP27 and HSP70 overexpression i

关键词： Esophageal adenocarcinoma Heat shock proteins Heat shock protein 27 Heat shock protein 70 Overexpression Survival

Crohn’s disease:Innate immunodeficiency?

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World Journal of Gastroenterology 2006年第42期12卷 6751-6755页

作者： Jesus K Yamamoto-Furusho Joshua r Korzenik Gastro-intestinal unit and Center for the Study of Inflammatory Bowel Disease Massachusetts General Hospital and Harvard Medical School Boston MA 02114 United States

In the past, Crohn’s disease (CD) has been understood primarily as an immunologic disorder characterized by an abnormal T-cell response. recent in vitro and in vivo data suggests that CD may instead be precipitated by innate immune dysfunction resulting from a combination of genetic and environmental factors. Some reports have demonstrated a defective immune response in a variety of other cellular components, including neutrophils, monocytes and dendritic cells. recent studies of granulocyte-macrophage colony-stimulating factor (GM- CSF) in CD, aiming to stimulate the innate immune system with the conception that an innate immune defect underlies the development of the disease, have been demonstrated a clinical benefit and reinforce this evolving understanding of the disease.

关键词：结肠疾病免疫机制治疗临床

老年心绞痛患者细胞粘附分子测定及调脂治疗观察

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实用老年医学 2001年第6期15卷 314-316页

作者：朱世明吕以杰白雪孙晓辉山东大学临床医学院济南市中心医院济南市250013

目的　探讨老年心绞痛患者冠状动脉粥样病变炎症反应及调脂干预对其产生的影响。　方法　测定 60例老年心绞痛患者和 3 0例健康老人的血清P 选择素、氧化修饰低密度脂蛋白 (OX LDL)、一氧化氮 (NO)水平并对病例组给予辛伐他汀调脂治疗... 详细信息

目的　探讨老年心绞痛患者冠状动脉粥样病变炎症反应及调脂干预对其产生的影响。　方法　测定 60例老年心绞痛患者和 3 0例健康老人的血清P 选择素、氧化修饰低密度脂蛋白 (OX LDL)、一氧化氮 (NO)水平并对病例组给予辛伐他汀调脂治疗。　结果　老年心绞痛组血清P 选择素 (1 92± 0 88)mg·L-1、OX LDL(1 5 4±0 5 6)mg·L-1比对照组 (0 90± 0 3 2 )mg·L-1、(0 76± 0 15 )mg·L-1明显增高 ,NO水平老年心绞痛组为 (68 5 2± 13 41)mmol·L-1,对照组 (119 88± 2 6 85 )mmol·L-1,明显降低 (P r =0 65 ,P r =-0 72 ,P <0 0 1)。调脂治疗后病例组血清P 选择素、OX LDL水平明显降低 (1 2 6± 0 3 8)mg·L-1、(0 92± 0 3 3 )mg·L-1,P <0 0 1,NO水平明显回升 (113 2 6± 2 7 75 )mmol·L-1,P<0 0 1。　结论　老年心绞痛患者存在冠状动脉粥样病变炎症反应。

关键词：心绞痛 P-选择素细胞粘附分子调脂治疗

Gold nanoshells： Contrast agents for cell imaging by cardiovascular optical coherence tomography

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Nano research 2018年第2期11卷 676-685页

作者： Jie Hu Francisco Sanz-rodríguez Fernando rivero Emma Martín rodríguez río Aguilar Torres Dirk H. Ortgies José García Solé Fernando Alfonso Daniel Jaque Fluorescence Imaging Group Departamento de Física de Materiales Instituto Nicolás Cabrera Facultad de Ciencias Universidad Autónoma de Madrid Madrid Spain Instituto ramón y Cajal de Investigación Sanitaria Hospital ramón y Cajal Madrid Spain departamento de Biología universidad Autónoma de Madrid Madrid Spain Cardiology depar.ment Hospital universitario de la Princesa IIS-IP Universidad Autónoma de Madrid Madrid Spain

Optical coherence tomography （OCT） has gained considerable attention in interventional cardiovascular medicine and is currently used in clinical settings to assess atherosclerotic lesions and to optimize stent placement. Artery imaging at the cellular level constitutes the first step towards cardiovascular molecular imaging, which represents a major advance in the development of personalized noninvasive therapies. In this work we demonstrate that cardiovascular OCT can be used to detect individual cells suspended in biocompatible fluids. Importantly, the combination of this catheter-based clinical technique with gold nanoshells （GNSs） as intracellular contrast agents led to a substantial enhancement in the backscattered signal produced by individual cells. This cellular contrast enhancement was attributed to the large backscattering cross-section of GNSs at the OCT laser wavelength （1,300 nm）. A simple intensity analysis of OCT cross-sectional images of suspended cells makes it possible to identify the sub-population of living cells that successfully incorporated GNSs. The generalizability of this method was demonstrated using two different cell lines （HeLa and Jurkat cells）. This work provides novel insights into cardiovascular molecular imaging using specifically modified GNSs.

关键词： optical coherencetomography (OCT)intravascular imaging gold nanoparticles three-dimensional (3D)imaging

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急性甲型肝炎T细胞亚群的变化

上海医学 1989年第6期12卷 335-337页

作者：张鲁榕孔宪涛祝念林张浩马健张玲珍瞿瑶高景阳周明行徐沪济第二军医大学附属长征医院实验科第二军医大学附属长征医院传染科

应用流式细胞仪和OKT McAb分析了30例急性甲型肝炎外周血T细胞亚群变化,并观察其中5例的动态变化。结果表明,病程极期患者CD_4亚群百分率明显降低;CD_6亚群百分率升高;CD_4/CD__8比值明显降低。这些变化可能与HAV侵害淋巴系统及应激性... 详细信息

应用流式细胞仪和OKT McAb分析了30例急性甲型肝炎外周血T细胞亚群变化,并观察其中5例的动态变化。结果表明,病程极期患者CD_4亚群百分率明显降低;CD_6亚群百分率升高;CD_4/CD__8比值明显降低。这些变化可能与HAV侵害淋巴系统及应激性免疫调动有关。

关键词：甲型肝炎 T细胞亚群变化