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A Fluorescent Alternative to the Synthetic Strigolactone GR24

Molecular Plant 2013年第1期6卷 100-112页

作者： Amanda Rasmussen Thomas Heugebaert cedrick matthys Rik Van Deun Francois-Didier Boyer1 Sofie Goormachtig Christian Stevens Danny Geelen Plant Production Faculty of Bioscience Engineering Ghent University Coupure links 653 Ghent 9000 Belgium Research Group SynBioC Department of Sustainable Organic Chemistry and Technology Faculty of Bioscience Engineering Ghent University Coupurelinks 653 B-9000 Gent Belgium Department of Plant Systems Biology VIB Ghent 9052 Belgium Department of Plant Biotechnologyand Bioinformatics Ghent University Ghent 9052 Belgium f-element Coordination Chemistry Lab Inorganic and Physical Chemistry Department Ghent University Krijgslaan 281--$3 9000 Gent Belgium Centre de Recherche de Gif Institut de Chimie des Substances Naturelles UPR2301 CNRS INRA 1 avenue de la Terrasse F-91198 Gif-sur-Yvette Cedex France

Strigolactones have recently been implicated in both above- and below-ground developmental pathways in higher plants. To facilitate the molecular and chemical properties of strigolactones in vitro and in vivo, we have devel- oped a fluorescent strigolactone molecule, CISA-1, synthesized via a novel method which was robust, high-yielding, and used simple starting materials. We demonstrate that CISA-1 has a broad range of known strigolactone activities and further report on an adventitious rooting assay in Arabidopsis which is a highly sensitive and rapid method for testing biological activity of strigolactone analogs. In this rooting assay and the widely used Orobanche germination assay, CISA-1 showed stronger biological activity than the commonly tested GR24. CISA-1 and GR24 were equally effective at inhibiting branching in Arabidopsis inflorescence stems. In both the branching and adventitious rooting assay, we also demonstrated that CISA-1 activity is dependent on the max strigolactone signaling pathway. In water methanol solu- tions, CISA-1 was about threefold more stable than GR24, which may contribute to the increased activity observed in the various biological tests.

关键词： strigolactones adventitious rooting branching fluorescent markers parasitic weed seed germination.

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