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Comparison of inconclusive rates between suction rectal biopsy and open strip rectal biopsy in children of different age groups:a single-center retrospective study

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World Journal of Pediatric Surgery 2020年第1期3卷 6-11页

作者： cedric ian ng liet hing Roy Teng Liesel Porrett Richard Thompson Paediatric Surgery Gold Coast University HospitalSouthportQueenslandAustralia

background Rectal biopsy for the diagnosis for Hirschsprung's disease(HD)can be performed in several *** rectal biopsy(SRB)is the most widely used method for neonates and younger infants while open strip biopsy(OSB)is reserved for older *** notions suggest that SRB should not be used in older infants due to perceived thicker fibrous tissue in their rectal walls leading to higher rates of inconclusive *** study aims to compare the inconclusive rates of both methods in children of different age *** A retrospective study were carried out with patients aged 13 years who underwent SRB or OSB during a 4-year period in a single *** biopsies were performed on patients with HD with previous endorectal pull-through surgeries *** outcomes were rates of inconclusive results for SRB and OSB overall and when divided into different age *** 79 biopsies(57 SRB and 22 OSB)were included in the study.12 biopsies(9 SRB and 3 OSB)were deemed *** was no significant difference in the rate of inconclusive results between patients underwent SRB and OSB overall(15.8%vs 13.6%,p=1.000).The same results were obtained when patients were divided into under one year and over one year groups or other different age groups(30.0%vs 33.3%,p=1.000).Conclusions Despite low biopsy numbers,our study suggests that SRB provides comparable rates of inconclusive results with OSB in children of all age groups.

关键词： younger walls thick

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The Fate of Aflatoxin in Corn Fermentation

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Natural Resources 2012年第3期3卷 126-136页

作者： C. ian Johnston Rebecca Singleterry cedric Reid Darrell Sparks Ashli Brown Brian Baldwin Stephanie Hill Ward W. Paul Williams Corn Host Plant Resistance Research Unit United States Department of Agriculture Agricultural Research Service Washington DC USA Department of Animal and Dairy Science Mississippi State University Oktibbeha County USA Department of Biochemistry Molecular Biology Entomology and Plant Pathology Mississippi State University Oktibbeha County USA Department of Plant and Soil Sciences Mississippi State University Oktibbeha County USA

A lab-scale ethanol fermentation was investigated to determine where aflatoxin concentrated during each phase of production. Four corn samples with high levels of aflatoxin (ranging from 7750 – 17,208 parts per billion) and their replicates were compared with a replicated negative control. Fractions were taken from the fermented mash, distilled ethanol, stillage, and dried corn solids (DCS). These fractions were analyzed using two different immunoassay methods and liquid chromatography tandem mass spectrometry (LC-MS/MS). Results indicated no aflatoxin was found in the distilled ethanol. Some aflatoxin (13%) was detected in the stillage, but most of the toxin was recovered in the DCSs ranging from 31% to 58%. A second series of experiments were conducted to investigate the effect of binders on dried distillers grains (DDGs). A brewers dried yeast anti-caking binder that contains glucomannon (MTB-100?), was mixed with contaminated DDGs. Addition of the binder showed a significant reduction in aflatoxin levels in comparison to a positive control. Aflatoxin binding at 2% binder w/w reached 72.5% and showed a minimal binding percentage increase of 80% at 6% binder w/w. Testing was also conducted to determine if environmental variables such as pH and temperature had any effect on the binding capabilities. Temperature near 0?C resulted in binding at 19.7% at a pH range of 6 to 8. Additionally, at a temperature of 40?C resulted in binding of 36%, 47%, and 45% at pHs 6, 7, and 8, respectively. These findings suggest that the addition of sorbents may be an effective way of salvaging contaminated DDGs.

关键词： LC-MS/MS Ethanol Immunoassay Distillers Grains Binders

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