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Oral administration of Sauce llorón extract to growing lambs to control gastrointestinal nematodes and Moniezia spp.

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Asian Pacific journal of Tropical Medicine 2015年第7期8卷 518-523页

作者： cedillo j Kholif AE Salem AZM Elghandour MMY Vázquez jF Alonso MU Barbabosa A Chagoyán jCV Reyna AG University center of UAEM-Temascaltepec Autonomous University of the Mexico State Dairy Science Department National Research Centre Faculty of Veterinary Medicine and Animal Science Autonomous University of the Mexico State Autonomous University of the Tamaulipas Faculty of Engineering and Science

Objective: To explore anthelmintic effects of oral administration of aqueous extract of Sauce llorón(Salix babylonica; SB) against gastrointestinal nematodes and Moniezia spp. Methods: Sixteen Pelibuey male lambs of 3-4 months of age and(23.7±3.3) kg body weight were used in a completely randomized design to be fed a total mixed ration(Control; SB0), or Control plus SB extract using 20(SB20), 40(SB40) and 60(SB60) mL/lamb/day for 45 days. Lambs had a natural gastrointestinal nematodes and Moniezia spp. infection and had never been treated with chemical anthelmintic drugs. Individual faecal samples were collected for ova counting using Mc Master procedure after 0, 7, 14, 21, 30 and 45 days post extract administration. Results: No extract dose×day interactions for both gastrointestinal nematodes and Monieziaspp. egg count were found. Administration of SB extract had a higher effect(quadratic effect, P=0.0064) at dose of 20 mL SB/lamb/day for gastrointestinal nematode eggs during the first 21 days; however, the dose of SB40 tended(linear effect, P=0.0897) to be more effective than the others for Moniezia spp. egg during the first 7 days. Sampling day had a linear(P=0.0436) effect on Moniezia spp. egg count. Conclusions: The aqueous extract of SB could be more effective against nematodes at 20 and at 40 mL/lamb/day for Moniezia spp. The use of the SB extract could represent a promising alternative to synthetic anthelmintics for the treatment of gastrointestinal nematodes and Moniezia spp. in small ruminants from organic and conventional production systems.

关键词： Anthelmintic Extract Moniezia spp. Nematodes

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Detection of Mycoplasmas in Patients with Amyotrophic Lateral Sclerosis

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Advances in Microbiology 2014年第11期4卷 712-719页

作者： Constantino Gil Alma Aurora Sánchez González Isidro Lecona León Antonio Rivera Rayo Santellán Olea Lilia cedillo Centro de Investigaciones en Ciencias Microbiológicas Instituto de Ciencias Benemérita Universidad Autónoma de Puebla Edificio 103J Ciudad Universitaria Col. San Manuel Puebla Pue México Escuela de Biología Benemérita Universidad Autónoma de Puebla Edificio 112A Ciudad Universitaria Col. San Manuel Puebla Pue México Servicio de Neurología Centro Médico Nacional Manuel ávila Camacho IMSS Puebla México

Amyotrophic Lateral Sclerosis (ALS) is a progressive degenerative disease of the motor neurons and the cause is unknown. Diverse factors such as genetic defects, nutritional deficiencies, head trauma, environmental toxin, autoimmune responses and viral and bacterial infections are involved. Mycoplasmas have been implicated as causal agents of different illnesses in human. The purpose of this study was to investigate the presence of mycoplasmas in the bloodstream of patients with ALS. Patients with ALS and healthy individuals were included in the study. A blood sample was taken in tubes with or without anticoagulant. Mycoplasmas were detected by culture or direct PCR, and the presence of antibodies IgM and IgG against LAMPs of these microorganisms by Western blot. Cultures for aerobic facultative bacteria were also done. Blood samples from 13 patients and 44 healthy individuals were screened. All blood cultures for non-fermentative mycoplasmas and aerobic facultative bacteria were negative. Cultures for fermentative mycoplasmas were considered positive after identification of mycoplasmal DNA by PCR. Mycoplasma sp. was detected by culture or direct PCR in 6/13 (46%) patients and in 4/44 (9%) of healthy individuals. M. fermentans was detected by PCR using specific primers in six patients and in two healthy individuals. IgM against LAMPs of M. fermentans were detected in 6/13 (46%) blood samples from patients and in 13/44 (30%) from healthy individuals, while. IgG was detected in 4/13 (31%) patients and in 3/44 (7%) healthy individuals. The results of this study show that mycoplasmas cause a systemic infection and could play a role in the origin or progression of the ALS.

关键词： Amyotrophic Lateral Sclerosis Mycoplasmas Immune Response

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超声多普勒对25例疖肿型蝇蛆病的诊断价值

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世界核心医学期刊文摘（皮肤病学分册） 2005年第6期1卷 60-60页

作者： Quintanilla-cedillo M.R. Lénaon-Ure.H. Contreras-Ruiz j. Arenas R. 张宪旗 Dr.Hosp. Gen. Dr. Manuel Gea Gonzalez Calzada de Tlalpan 4800 Tlalpan D.F. 14000 Mexico

Background. The larvae of the botfly Dermatobia hominis cause furunculoid myiasis in endemic areas. Lack of knowledge of this condition outside these areas leads to confusion in diagnosis and delays appropriate treatment. Objective. To describe the clinical findings of furunculoid myiasis encountered in Quintana Roo, in the south-east of Mexico. Materials and methods. We performed an observational study on 25 cases diagnosed over a period of 4 years. Results. Diagnosis of furunculoid myiasis was made in 14 males and 11 females with an average age of 24.5 years. Most of our patients were students, farmers, or housewives. The number of lesions varied from one to four. In 20% of cases, more than one parasite was present in each lesion. The scalp was the most commonly affected region (40% ), followed by the trunk and the extremities. Doppler ultrasound study (DUSG) of the furuncle- like lesions confirmed the clinical diagnosis in all cases. Conclusions. Furunculoid myiasis is frequent in the state of Quintana Roo, Mexico. We found no association with occupation, gender, social background or age. DUSG can be used to evaluate the number of parasites per furunculoid lesion avoiding misdiagnoses and treatmentdelays.

关键词：蝇蛆病超声多普勒肤蝇皮损数目非流行区临床诊断受累部位

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