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Mass spectrometry-based proteomics technology in pancreatic cancer research

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胰腺病学杂志(英文) 2024年第2期07卷 145-163页

作者： Sun Xue Wang Siyuan Wong catherine C.l. First School of Clinical Medicine Peking University Health Science Center Peking University Beijing China School of Pharmacy University of Wisconsin-Madison Madison Wisconsin United States State Key laboratory of Complex Severe and Rare Diseases Clinical Research Institute Peking Union Medical College Hospital Chinese Academy of Medical Science &amp Peking Union Medical College Beijing China Tsinghua-Peking University Joint Center for life Sciences Tsinghua University Beijing China

Pancreatic ductal adenocarcinoma (PDAC) has become a significant health concern with increasing incidence and mortality rates over the past few decades. Researchers have turned their attention to cutting-edge mass spectrometry (MS) technology due to its high-throughput and accurate detection capacity, which plays a vital role in understanding the mechanisms and discovering biomarkers for pancreatic diseases. In this review, we comprehensively investigate various methodologies of quantitative and qualitative proteomics MS technologies, alongside bioinformatical platforms employed in pancreatic cancer research. The integration of these optimized approaches provides novel insights into the molecular mechanisms underlying tumorigenesis and disease progression, ultimately facilitating the discovery of potential diagnostic, prognostic biomarkers, and therapeutic targets. The robust MS-based strategy shows promise in paving the way for early diagnosis and personalized medicine for pancreatic cancer patients.

关键词： Biomarkers Mass spectrometry Pancreatic cancer Pancreatic ductal adenocarcinoma Proteomics

Ultrasonic-Assisted Extraction of Fucoxanthin from Marine Macroalga Padina australis:Optimization,Bioactivity,and Structural Characterization

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Proceedings of Anticancer Research 2024年第5期8卷 89-104页

作者： l.Antony catherine Flora Kirubanandan Shanmugam Renganathan Sahadevan Department of Chemical Engineering Alagappa College of TechnologyAnna UniversityChennai 600025India Independent Research Professional ChennaiTamil NaduIndia Department of Biotechnology Alagappa College of TechnologyAnna UniversityChennai 600025India

Macroalgae serve as a potential feedstock for fucoxanthin ***,a bioactive pigment found in the chloroplasts of marine algae,exhibits significant pharmacological *** a member of the carotenoid family,fucoxanthin plays a crucial role in both the food and pharmaceutical *** research explores the effects of ultrasonics on the extraction of fucoxanthin from the marine macroalga Padina *** addition,various extraction techniques and the influence of solvents on the efficient separation of fucoxanthin from algae have been studied and *** methanol,chloroform,and a combination of methanol and chloroform(1:1,v/v),conventional fucoxanthin extraction from Padina australis yielded 8.12 mg of fucoxanthin per gram of ***,the ultrasonic-assisted extraction resulted in a significantly higher yield of 16.9 mg of fucoxanthin per gram of biomass,demonstrating that the use of ultrasonics enhances the extraction rate compared to conventional ***,the efficient separation of fucoxanthin from Padina australis is highly dependent on ultrasonic-assisted *** process conditions for the extraction were optimized to maximize the yield of fucoxanthin from *** following parameters were selected for optimization studies:moisture content,particle size,mixing speed,extraction temperature,extraction duration,and solid-to-solvent *** extracted fucoxanthin exhibited various biological activities,including antimicrobial and antioxidant properties,and its structure was elucidated through FTIR and NMR ***,thin-layer chromatography of the crude algae extracts confirmed the presence of fucoxanthin in the marine *** these findings,the optimized extraction process holds the potential for scaling up to large-scale fucoxanthin ***,as a potent pharmacological agent,offers promising applications in the treatment of various ailments.

关键词： Marine algae Fucoxanthin Ultrasonics Extraction Optimization TlC DPPH Antimicrobial antioxidant activities

limosilactobacillus mucosae FZJTZ26M3 prevents NAFlD in mice through modulation of lipid metabolism and gut microbiota dysbiosis

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Food Science and Human Wellness 2024年第3期13卷 1589-1601页

作者： Danting Dang Bowen li Mengfan Ding RPaul Ross catherine Stanton Jianxin Zhao Bo Yang Wei Chen State Key laboratory of Food Science and Resources Jiangnan UniversityWuxi 214122China School of Food Science and Technology Jiangnan UniversityWuxi 214122China International Joint Research Center for Probiotics&Gut Health Jiangnan UniversityWuxi 214122China APC Microbiome Ireland University College CorkCork T12 YT20Ireland Teagasc Food Research Centre MooreparkFermoyCork T12 YT20Ireland National Engineering Research Center for Functional Food Jiangnan UniversityWuxi 214122China

lactobacillus are considered promising therapeutic methods for nonalcoholic fatty liver disease(NAFlD).The effects of two strains of ltmosilactobacillus mucosae on NAFlD were investigated in this ***-old male C57Bl/6J mice were divided into 4 groups(n=8 per group,Control,Model,FZJTZ26M3,FGSYC17l3).*** FZJTZ26M3 reduced the mice 's body weight,liver weight,and adipose tissue weight after 12 weeks of *** to serum analysis,total cholesterol,triacylglycerol,and low-density lipoprotein cholesterol significantly decreased after *** FZJTZ26M3 *** pathology showed that *** FZJTZ26M3 was effective to ameliorate lipid deposition in NAFlD ***,the expression of the gene related to lipid metabolism in the liver and adipose tissue was analyzed,and the results indicated that *** FZJTZ26M3 could alleviate NAFlD by regulating lipid ***,the results of 16S rRNA gene sequencing revealed a drop in the relative abundance of Ruminococcaceae,which is linked to inflammation,but the relative abundance of a potential probiotic Akkermansia significantly increased after *** FZJTZ26M3 ***,*** FZJTZ26M3 could be a candidate to prevent NAFlD.

关键词： limosilactobacillus mucosae Nonalcoholic fatty liver disease(NAFlD) Probiotic lipid metabolism Gut microbiota

Unsynchronized butyrophilin molecules dictate cancer cell evasion of Vγ9Vδ2 T-cell killing

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Cellular & Molecular Immunology 2024年第4期21卷 362-373页

作者： Zeguang Wu Qiezhong lamao Meichao Gu Xuanxuan Jin Ying liu Feng Tian Ying Yu Pengfei Yuan Shuaixin Gao Thomas S.Fulford Adam P.Uldrich catherine C.l Wong Wensheng Wei Biomedical Pioneering Innovation Center Peking-Tsinghua Center for Life SciencesPeking University Genome Editing Research CenterState Key Laboratory of Protein and Plant Gene ResearchSchool of Life SciencesPeking University100871BeijingChina EdiGene Inc. Life Science ParkChangping District102206BeijingChina Center for Precision Medicine Multi-Omics Research Peking University Health Science CenterPeking University100191BeijingChina Department of Microbiology and Immunology at the Peter Doherty Institute for Infection and Immunity The University of MelbourneParkvilleVIC3010Australia Cancer Immunology Program Peter MacCallum Cancer CentreMelbourneVICAustralia State Key laboratory for Complex Severe and Rare DiseasesClinical Research InstitutePeking Union Medical College HospitalChinese Academy of Medical Science&Peking Union Medical CollegePeking-Tsinghua Center for Life Sciences100871BeijingChina

Vγ9Vδ2 T cells are specialized effector cells that have gained prominence as immunotherapy agents due to their ability to target and kill cells with altered pyrophosphate *** our effort to understand how cancer cells evade the cell-killing activity of Vγ9Vδ2 T cells,we performed a comprehensive genome-scale CRISPR screening of cancer *** found that four molecules belonging to the butyrophilin(BTN)family,specifically BTN2A1,BTN3A1,BTN3A2,and BTN3A3,are critically important and play unique,nonoverlapping roles in facilitating the destruction of cancer cells by primary Vγ9Vδ2 T *** coordinated function of these BTN molecules was driven by synchronized gene expression,which was regulated by IFN-γsignaling and the RFX ***,an enzyme called QPCTl was shown to play a key role in modifying the N-terminal glutamine of these BTN proteins and was found to be a crucial factor in Vγ9Vδ2 T cell killing of cancer *** our research,we offer a detailed overview of the functional genomic mechanisms that underlie how cancer cells escape Vγ9Vδ2 T ***,our findings shed light on the importance of the harmonized expression and function of gene family members in modulating T-cell activity.

关键词： Butyrophilin Vγ9Vδ2 T-cell Cancer-specific immune evasion Glutaminyl-peptide cyclotransferase-like Pyrophosphate metabolite Immunotherapy

Intraperitoneal administration of butyrate prevents the severity of acetic acid colitis in rats

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2015年第3期16卷 224-234页

作者： Joshua J.MAlAGO catherine l.SANGU Department of Pathology Faculty of Veterinary MedicineSokoine University of Agriculture c/o Walter Oseko

Intrarectal infusion of butyrate improves colorectal disorders including ulcerative colitis （UC）. However, it is not established whether systemically administered butyrate benefits such patients. The current study aimed at ex- ploring and comparing the potential of intraperitoneally, intrarectally, and orally administered butyrate against acetic acid （AA）-induced UC in rats. Intrarectal administration of 2 ml of 50% AA was done after or without prior treatment of rats for 7 consecutive days with 100 mg/kg sodium butyrate （SB） intraperitoneally, intrarectally, or orally. Rats were sacrificed after 48 h of hA-treatment. Subsequently, colon sections were processed routinely for histopathological examination. We clinically observed diarrhea, loose stools, and hemoccult-positive stools, and histologically, epithelial loss and ulceration, crypt damage, goblet cell depletion, hemorrhage, and mucosal infiltration of inflammatory cells. The changes were significantly reduced by intraperitoneal, intrarectal, or oral butyrate, with intraperitoneal butyrate exhibiting the highest potency. It is concluded that intraperitoneal administration of butyrate abrogates the lesions of hA-induced UC and its potency surpasses that of intrarectal or oral butyrate.

关键词： Butyrate Oral administration Intraperitoneal administration Intrarectal administration Acetic acid,Ulcerative colitis

Animal models for the study of hepatitis C virus infection and replication

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World Journal of Gastroenterology 2012年第23期18卷 2909-2913页

作者： Kristin l MacArthur catherine H Wu George Y Wu Department of Medicine Beth Israel Deaconess Medical Center Boston MA 02215 United States Department of Medicine Division of Gastroenterology-Hepatology University of Connecticut Health Center Farmington CT 06030-1845 United States

Hepatitis C virus (HCV) hepatitis, initially termed non-A, non-B hepatitis, has become one of the leading causes of cirrhosis and hepatocellular carcinoma worldwide. With the help of animal models, our understanding of the virus has grown substantially from the time of initial discovery. There is a paucity of available animal models for the study of HCV, mainly because of the selective susceptibility limited to humans and primates. Recent work has focused modification of animals to permit HCV entry, replication and transmission. In this review, we highlight the currently available models for the study of HCV including chimpanzees, tupaia, mouse and rat models. Discussion will include methods of model design as well as the advantages and disadvantages of each model. Particular focus is dedicated to knowledge of pathophysiologic mechanisms of HCV infection that have been elucidated through animal studies. Research within animal models is critically important to establish a complete understanding of HCV infection, which will ultimately form the basis for future treatments and prevention of disease.

关键词： Hepatitis C virus Infection Replication Vac-cine Hepatitis A virus

Vinyl chloride monomer production catalysed by gold: A review

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Chinese Journal of Catalysis 2016年第10期37卷 1600-1607页

作者： catherine J.Davies Peter J.Miedziak Gemma l.Brett Graham J.Hutchings 卡迪夫大学

In this review we discuss the history of research into the use of gold for the acetylene hydrochlorin‐ation reaction, and describe the recent developments which have led to its commercialisation. We discuss the use of different precursors and the addition to gold of a secondary metal as methods which attempt to improve these catalysts, and consider the nature of the active gold species. The vast majority of poly vinyl chloride （PVC） produced globally still uses a mercuric chloride as a cata‐lyst, despite the environmental problems associated with it. Due to the agreement by the Chinese government to remove mercury usage in the PVC industry over the course of the next few years there is an obvious need to find a replacement catalyst;the potential use of gold for this process has been well known for several decades and to date gold seems to be the best candidate for this, pri‐marily due to its superior selectivity when compared to other metals.

关键词： Gold Acetylene Hydrochlorination Vinyl chloride monomer Poly vinyl chloride Catalysis

Challenges for cysteamine stabilization,quantification,and biological effects improvement

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Journal of Pharmaceutical Analysis 2020年第6期10卷 499-516页

作者： Carla Atallah catherine Charcosset Helene Greige-Gerges Bioactive Molecules Research laboratory Doctoral School of Sciences and TechnologiesFaculty of SciencesLebanese UniversityLebanon laboratory of Automatic Control Chemical and Pharmaceutical EngineeringClaude Bernard Lyon 1 UniversityFrance

The aminothiol cysteamine,derived from coenzyme A degradation in mammalian cells,presents several biological ***,the bitter taste and sickening odor,chemical instability,hygroscopicity,and poor pharmacokinetic profile of cysteamine limit its *** use of encapsulation systems is a good methodology to overcome these undesirable properties and improve the pharmacokinetic behavior of ***,the conjugation of cysteamine to the surface of nanoparticles is generally proposed to improve the intra-oral delivery of cyclodextrin-drug inclusion complexes,as well as to enhance the colorimetric detection of compounds by a gold nanoparticle aggregation *** the other hand,the detection and quantification of cysteamine is a challenging mission due to the lack of a chromophore in its structure and its susceptibility to oxidation before or during the *** agents are therefore applied for the quantification of this *** our knowledge,the derivatization techniques and the encapsulation systems used for cysteamine delivery were not reviewed ***,this review aims to compile all the data on these methods as well as to provide an overview of the various biological applications of cysteamine focusing on its skin application.

关键词： Cysteamine Detection Encapsulation Skin Stability

Sol–gel la2O3–ZrO2 mixed oxide catalysts for biodiesel production

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Journal of Energy Chemistry 2018年第2期27卷 565-572页

作者： Daniela Salinas catherine Sepúlveda Néstor Escalona J.l.GFierro Gina Pecchi Departamento de Fisicoquimica. Facultad de Ciencias Quimicas Universidad de Concepcion Post Office 160-C Concepcion Chile Departamento de Quimica Facultad de Ciencias Universidad del Biobio Post Office 5-C Concepcion Chile Departamento Ingenieria Quimica y Bioprocesos Escuela de lngenieria Pontificia Universidad Catolica de Chile Avda. Vicuria Mackenna 4870 Maeul Santiago 7820436 Chile Centro de Investigacion en Nanotecnolagia y Materiales Avanzados (CIEN-UC) Pontificia Universidad Catolica de Chile Post Office 306 Santiago Chile Departamento de Quimica Fisica Facultad de Quimica Pontificia Universidad Catolica de Chile Post Offce 306 Santiago Chile Instituto de Catalisis y Petroleoquimica CSIC 28049 Madrid. Espana

Different la2O3 contents（0, 1, 2, 3, and 5 wt%） were used to prepared la2O3-ZrO2 mixed oxides calcined at 600 ℃ by the sol-gel method. The catalytic activity was measured as biodiesel production from canola oil through a transesterification reaction. The characterization results indicate that the la2O3 monolayer formation and extent of basicity of m-ZrO2 have a large influence on biodiesel *** biodiesel conversion（56% at 4 h） was obtained with the 3% la2O3-ZrO2 catalyst in the presence of basic sites and the formation of a monolayer of la2O3. The decrease in the catalytic activity for 5% la2O3-ZrO2 resulted from the loss of active sites on the catalyst because of agglomeration, which was suggested by XPS and the isoelectric point. The kinetic data fit to a pseudo-first order constant, and the largest kinetic constant corresponds to 3% la2O3-ZrO2, currently the largest heterogeneous non-alkaline metal catalyst reported for a transesterification reaction.

关键词： la2O3 -ZrO2 Sol-gel Biodiesel Canola oil Transesterification