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RNA Secondary Structures Located in the Interchromosomal Region of Human ACAT1 Chimeric mRNA are Required to Produce the 56-kDa Isoform

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RNA Secondary Structures Located in the Interchromosomal Reg...

2008年全国生物化学与分子生物学学术大会

作者： catherine cy chang Department of Biochemistry Dartmouth Medical SchoolHanoverNH 03756USA

We have previously reported that the human ACAT1 gene produces a chimeric mRNA through the inter- chromosomal processing of two discontinuous RNAs transcribed from chromosomes 1 and *** chimeric mRNA uses AUG and GGC ...

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Enhancement of human ACAT1 gene expression to promote the macrophage-derived foam cell formation by dexamethasone

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Cell Research 2004年第4期14卷 315-323页

作者： Li YANG, Jin Bo YANG, Jia CHEN, Guang Yao YU, Pei ZHOU, Lei LEI, Zhen Zhen WANG, catherine cy chang, XinYing YANG, Ta Yuan chang, Bo Liang LI State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Graduate School of the Chinese Academy of Sciences, 320 Yueyang Road Shanghai 200031, China. Department of Biochemistry, Dartmouth Medical School, Hanover, NH 03756 USA. State Key Laboratory of Molecular Biology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Graduate School of the Chinese Academy of Sciences Shanghai China Department of Biochemistry Dartmouth Medical School Hanover USA

In macrophages, the accumulation of cholesteryl esters synthesized by the activated acyl-coenzyme A:cholesterol acyltransferase-1 (ACAT1) results in the foam cell formation, a hallmark of early atherosclerotic lesions. In this study, with the treatment of a glucocorticoid hormone dexamethasone (Dex), lipid staining results clearly showed the large accumulation of lipid droplets containing cholesteryl esters in THP-1-derived macrophages exposed to lower concentration of the oxidized low-density lipoprotein (ox-LDL). More notably, when treated together with specific anti-ACAT inhibitors, the abundant cholesteryl ester accumulation was markedly diminished in THP-1-derived macrophages, confirming that ACAT is the key enzyme responsible for intracellular cholesteryl ester synthesis. RT-PCR and Western blot results indicated that Dex caused up-regulation of human ACAT1 expression at both the mRNA and protein levels in THP-1 and THP-1-derived macrophages. The luciferase activity assay demonstrated that Dex coul

关键词： ACAT dexamethasone macrophage cholesteryl ester gene promoter.

A novel exo-endo RNA trans-splicing to produce ACAT1 isoform in human cells involving the ampicillin resistance gene

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A novel exo-endo RNA trans-splicing to produce ACAT1 isoform...

第十一次全国基因功能与表观遗传调控学术研讨会

作者： Guang-Jing Hu Jia Chen Xiao-Nan Zhao Jia-Jia Xu Dong-Qing Guo Ming Lu Ming Zhu Ying Xiong Qin Li catherine cy chang Bao-Liang Song Ta-Yuan chang Bo-Liang Li State Key Laboratory of Molecular Biology Institute of Biochemistry and Cell BiologyShanghai Institutes for Biological SciencesChinese Academy of Sciences

Trans-splicing,a process involving the cleavage and joining of two separate transcripts,can expand the transcriptome and proteome in *** RNAs generated by trans-splicing are increasingly described in *** widespread presence of antibiotic resistance genes in natural environments and human intestines is becoming an important challenge for public health.

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Membrane-bound O-acyltransferases(MBOATs)

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Frontiers in Biology 2011年第3期6卷 177-182页

作者： catherine C.Y.chang Jie SUN Ta-Yuan chang Department of Biochemistry Dartmouth Medical SchoolHanoverNH 03755 USA

The MBOATenzyme family,identified in 2000,comprises 11 genes in the human genome that participate in a variety of biological *** enzymes contain multiple transmembrane domains and share two active site residues,histidine and *** MBOAT members are drug targets for major human diseases,including atherosclerosis,obesity,Alzheimer disease,and viral *** we review the historical aspects of MBOAT enzymes,classify them biochemically into 3 subgroups,and describe the essential features of each member.

关键词： cholesterol metabolism neutral lipid biosynthesis protein acylation membrane phospholipid remodeling atherosclerosis diabetes obesity cancer nutrient sensing

Robust genome and RNA editing via CRISPR nucleases in PiggyBac systems

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Bioactive Materials 2022年第8期7卷 313-320页

作者： Yuqian Jiang Rachel catherine Hoenisch Yun chang Xiaoping Bao Craig E.Cameron Xiaojun Lance Lian Department of Biomedical Engineering Pennsylvania State UniversityUniversity ParkPA16802USA Huck Institutes of the Life Sciences Pennsylvania State UniversityUniversity ParkPA16802USA Department of Biology Pennsylvania State UniversityUniversity ParkPA16802USA Davidson School of Chemical Engineering Purdue UniversityWest LafayetteIN47907USA Department of Microbiology and Immunology University of North Carolina School of MedicineChapel HillNC27599USA

CRISPR/Cas-mediated genome editing in human pluripotent stem cells(hPSCs)offers unprecedented opportunities for developing in vitro disease modeling,drug screening and cell-based *** efficiently deliver the CRISPR components,here we developed two all-in-one vectors containing Cas9/gRNA and inducible Cas13d/gRNA cassettes for robust genome editing and RNA interference *** vectors utilized the PiggyBac transposon system,which allows stable expression of CRISPR components in *** Cas9 vector PB-CRISPR exhibited high efficiency(up to 99%)of inducing gene knockout in both protein-coding genes and long non-coding *** other inducible Cas13d vector achieved extremely high efficiency in RNA knockdown(98%knockdown for CD90)with optimized gRNA *** together,our PiggyBac CRISPR vectors can serve as powerful toolkits for studying gene functions in hPSCs.

关键词： CRISPR-Cas9 Genome editing Cas13d RNA editing PiggyBac transposon Human pluripotent stem cells

Human Acyl-CoA:cholesterol Acyltransferase (ACAT) and its Potential as a Target for Pharmaceutical Intervention against Atherosclerosis

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Acta Biochimica et Biophysica Sinica 2006年第3期38卷 151-156页

作者： catherine chang Ruhong DONG Akira MIYAZAKI Naomi SAKASHITASecond Department of Pathology,Kumamoto University School of Medicine,Kumamoto,Japan Jay LIU Michael GUO Department of Biochemistry Dartmouth Medical SchoolHanoverNew Hampshire 03755 USA Department of Biochemistry Showa University School of MedicineTokyoJapan

Acyl-CoA:cholesterol acyltransferase (ACAT) catalyzes the formation of cholesteryl estersfrom cholesterol and long-chain fatty-acyl-coenzyme *** the single-cell level,ACAT serves as a regulatorof intracellular cholesterol *** addition,ACAT supplies cholesteryl esters for lipoproteinassembly in the liver and small *** pathological conditions,the accumulation of cholesterylesters produced by ACAT in macrophages contributes to foam cell formation,a hallmark of the earlystage of *** reviews addressing various aspects of ACAT and ACAT inhibitors areavailable [1-8].This review briefly outlines the current knowledge on the biochemical properties of humanACATs,and then focuses on discussing the merit of ACAT as a drug target for pharmaceutical interventionsagainst atherosclerosis.

关键词： acyl-CoA cholesterol acyltransferase cholesterol cholesteryl ester atherosclerosis

Ultrasonographic visualization of lower eyelid structures and dynamic motion analysis

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International Journal of Ophthalmology(English edition) 2013年第5期6卷 592-595页

作者： Mohammad Taher Rajabi Konstantinos Papageorgiou Shu-Hong chang Imran Jarullazada Mehryar Taban catherine J.Hwang Robert Alan Goldberg Division of Orbital and Ophthalmic Plastic Surgery Jules Stein Eye InstituteUniversity of California-Los Angeles Eye Research Center Farabi Eye HospitalTehran University of Medical Sciences

AIMTo define the ultrasonographic structure of normal lower eyelid anatomic compartments and their spacial relationship in dynamic motion.

关键词： lower eyelid ultrasonography dynamic motion analysis

Cellular Cholesterol Esterification and Neurodegenerative Diseases

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Cellular Cholesterol Esterification and Neurodegenerative Di...

第三届中国生物物理学会代谢生物学分会学术研讨会

作者： catherine Chung Yao chang Ta Yuan chang Department of Biochemistry and Cell Biology Geisel School of Medicine at Dartmouth

＜正＞All cells require cholesterol,a portion of cellular cholesterol is stored as cholesterol esters;this process is catalyzed by the storage enzyme acyl-CoA:cholesterol acyltransferase 1 （ACAT1）.Once formed,cholesterol esters cannot substitute the functions of *** certain neurodegenerative diseases,needs for additional cholesterol in the brain *** AC AT1 may benefit these

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中国香港双语儿童初步阅读能力与语音、文字加工关系的研究

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心理科学 2002年第2期25卷 173-176页

作者：钟毅平 catherine Mcbride-chang Connie Suk-Han Ho 湘潭师范学院教育系湖南411201 香港中文大学心理系

采用音节检测、短时言语记忆、快速命名等语音加工任务和汉字、字母、数字等方向加工任务,探讨语音和文字加工在儿童初步阅读中的作用,116名6岁左右的香港双语儿童参加了测试。结果表明:语音加工能力是跨语言的比较稳定的因素,是预测汉... 详细信息

采用音节检测、短时言语记忆、快速命名等语音加工任务和汉字、字母、数字等方向加工任务,探讨语音和文字加工在儿童初步阅读中的作用,116名6岁左右的香港双语儿童参加了测试。结果表明:语音加工能力是跨语言的比较稳定的因素,是预测汉语和英语阅读的较好指标;文字加工具有特异性,即汉字方向加工只能预测汉语阅读,字母方向加工则预测英语阅读。

关键词：文字加工阅读能力字母方向言语记忆双语任务汉语英语阅读语音