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Novel cathelicidins with Potent Antimicrobial,Biofilm Inhibitory,and Anti-inflammatory Activities from the Frog Fejervarya multistriata

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Asian Herpetological Research 2017年第3期18卷 199-212页

作者： Aili WANG yan CHEN Haining YU Yipeng WANG Weifang University of Science and Technology Department of Pharmaceutical Sciences College of Pharmaceutical SciencesSoochow University Department of Bioscience and Biotechnology Dalian University of Technology

Antimicrobial peptides（AMPs）,a class of gene-encoded peptides,are the first line of immune system to defense microbial invasions in multicellular *** are an important family of AMPs that have been identified exclusively in ***,up to now,cathelicidins from amphibians are poorly *** the present study,we reported the identification and characterization of two novel cathelicidins（FM-cath1 and FMcath2） from the frog Fejervarya *** c DNA sequences encoding FM-caths were successfully cloned from the constructed lung c DNA library of *** of the c DNA sequences encoding FM-caths are 447 bp in length,and the deduced mature peptides of FM-caths are composed of 34 *** analysis indicated that FM-cath1 and FM-cath2 mainly assume amphipathic alpha-helical *** and bacterial killing kinetic analysis indicated that both FM-cath1 and FM-cath2 possess potent,broad-spectrum and rapid antimicrobial *** cytoplasmic membrane permeabilization analysis indicated that FM-cath1 and FMcath2 kill bacteria by inducing the permeabilization of bacterial *** direct antimicrobial activities,FM-caths also exhibited significant inhibitory effect on the formation of bacterial biofilms at low concentrations below 1×***,FM-cath1 and FM-cath2 exhibited potent anti-inflammatory activities by inhibiting LPS-induced transcription and production of pro-inflammatory cytokines TNF-α,IL-1β,and IL-6 in mouse peritoneal ***,FM-caths showed relatively low cytotoxic activity against mammalian normal and tumor cell lines,and low hemolytic activity against human *** summary,the identification of FM-caths provides novel clues for our understanding of the roles of cathelicidins in amphibian immune *** potent antimicrobial,biofilm inhibitory,anti-inflammatory activities,and low cytotoxicity of FM-caths imply their great potential in novel

关键词： cathelicidin Fejervarya multistriata FM-caths antimicrobial peptide bacterial biofilm antiinflammatory

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Molecular Cloning,Characterization and Expression Profile of Myf5 and Myf6 During Growth and Development in the Seriola lalandi

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Journal of Ocean University of China 2021年第6期20卷 1597-1605页

作者： SHI Bao SUN Ranran LIU Xuezhou ZHANG Zhengrong XU Yongjiang JIANG yan WANG Bin Key Laboratory of Sustainable Development of Marine Fisheries Ministry of Agriculture and Rural AffairsYellow Sea Fisheries Research InstituteChinese Academy of Fishery SciencesQingdao 266071China Laboratory for Marine Fisheries and Food Production Processes Pilot National Laboratory for Marine Science and Technology(Qingdao)Qingdao 266237China College of Fisheries and Life Science Dalian Ocean UniversityDalian 116023China

Myogenic Regulatory Factors(MRFs)is involved in the muscle growth and *** this study,the cDNA sequence of yellowtail kingfish MRFs genes were cloned by rapid amplification of cDNA ends(RACE)method;then,the character-istics of these genes and the predicted protein sequences were analyzed by bioinformatics methods,the tissue and embryonic stages differential expression pattern were detected by the quantitative real-time *** results showed that the yellowtail kingfish(YTK)Myf5 cDNA has a full length of 951 bp,encoding 266 amino *** yellowtail kingfish Myf6 cDNA has a full length of 1105bp,encoding 250 amino *** proteins containα-helix,β-strand,and *** Neighbour-joining tree revealed that YTK Myf5 and Myf6 are closely related to Seriola *** yellowtail kingfish Myf5 and Myf6 gene expressed significantly higher in mus-cle than in other tissues(P<0.05).In addition,Myf5 and Myf6 in muscle was significantly expressed in 400g and 500 g fish but not in 50 g,suggesting that myogenic regulatory factors expression had a great relationship with the fish *** results also indicated that Myf5 and Myf6 have different functions during embryonic development,because Myf5 showed highest expression level at the neuroembryo period,but Myf6 had the highest expression level at embryo coverage yolk 70%***5 gene showed highest ex-pression at 30 d of age,suggesting it played key roles in myogenic ***,the Myf6 gene was significantly highly ex-pressed at 60 d,revealing this gene functioned in the later muscle formation period.

关键词： Seriola lalandi myogenic regulatory factors gene cloning expression

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Degradation of 2,4-dichlorophenol catalyzed by the immobilized laccase with the carrier of Fe_3O_4@MSS–NH_2

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Chinese Science Bulletin 2014年第5期59卷 509-520页

作者： yan Huang yanjie Xi Yuxiang yang Cheng Chen Hongming Yuan Xiangnong Liu School of Chemistry and Molecular Engineering East China University of Science & Technology State Key Laboratory of Inorganic Synthesis and Preparative Chemistry College of Chemistry Jilin University Analysis Test Center Yangzhou University

With the Fe3O4@MSS(magnetic mesoporous silica spheres)–NH2as the carrier and the glutaraldehyde as crosslinking agent,the immobilized laccase has been prepared and characterized by XRD,IR,SEM and BET *** the optimal conditions,the removal efficiency of2,4-DCP reached 88%and the removal efficiency still remained 61.5%after five cycles of *** virtue of GC–MS analysis,2,5-dimethoxyl-1,4-quinone,2-chlorine-1,4-dimethoxyl benzene,3,30-dichlorine-4,40-dimethoxyl biphenyl,maleic acid phenol ester,and three kinds of maleic acid,alcohol ester,and para-hydroxyl phenol ester compounds have been identified as intermediate and final degradation products of 2,4-DCP,***,the degradation products of 2,4-DCP have been confirmed by performing the1H-NMR and13C-NMR experiments,further demonstrating the degradation mechanism of 2,4-DCP by the immobilized laccase.

关键词：二氯苯酚固定化酶降解 GC-MS分析 13C-NMR 载波催化 1H-NMR

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Deep-water Riser Fatigue Monitoring Systems Based on Acoustic Telemetry

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Journal of Ocean University of China 2014年第6期13卷 951-956页

作者： LI Baojun WANG Haiyan SHEN Xiaohong yan Yongsheng yanG Fuzhou HUA Fei School of Marine Science and Technology Northwestern Polytechnical University

Marine risers play a key role in the deep and ultra-deep water oil and gas production. The vortex-induced vibration （VIV） of marine risers constitutes an important problem in deep water oil exploration and production. VIV will result in high rates of structural failure of marine riser due to fatigue damage accumulation and diminishes the riser fatigue life. In-service monitoring or full scale testing is essential to improve our understanding of V1V response and enhance our ability to predict fatigue damage. One ma- rine riser fatigue acoustic telemetry scheme is proposed and an engineering prototype machine has been developed to monitor deep and ultra-deep water risers＇ fatigue and failure that can diminish the riser fatigue life and lead to economic losses and eco-catastrophe. Many breakthroughs and innovation have been achieved in the process of developing an engineering prototype machine. Sea trials were done on the 6th generation deep-water drilling platform HYSY-981 in the South China Sea. The inclination monitoring results show that the marine riser fatigue acoustic telemetry scheme is feasible and reliable and the engineering prototype machine meets the design criterion and can match the requirements of deep and ultra-deep water riser fatigue monitoring. The rich experience and field data gained in the sea trial which provide much technical support for optimization in the engineering prototype machine in the future.

关键词： marine riser fatigue monitoring engineering prototype machine underwater telemetry

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2018-2022年北京市海淀区游泳场所水质卫生状况分析

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中国卫生产业 2024年第10期21卷 10-13,21页

作者：刘雪莹胡咏梅孟田霍岩陈海平北京市海淀区疾病预防控制中心北京100094

目的了解北京市海淀区2018—2022年游泳池水卫生现状及变化趋势,发现存在的问题,提出有效的处理措施,提高游泳场所的水质卫生质量,保障居民游泳安全。方法北京市海淀区疾控中心于2018年6月—2022年9月选取北京市海淀区正常营业的423家... 详细信息

目的了解北京市海淀区2018—2022年游泳池水卫生现状及变化趋势,发现存在的问题,提出有效的处理措施,提高游泳场所的水质卫生质量,保障居民游泳安全。方法北京市海淀区疾控中心于2018年6月—2022年9月选取北京市海淀区正常营业的423家游泳场所为监测对象,采集游泳池水样,对样品的浑浊度、尿素、游离性余氯、pH值、细菌总数、大肠菌群6项指标进行测定,并对水质检测结果进行统计学分析。结果2018—2022年共检测游泳池水1269件,合格893件,总体合格率70.4%。2018年水质合格率为43.5%(146/336),2019年水质合格率为88.5%(292/330),2020年水质合格率为76.7%(69/90),2021年水质合格率为89.6%(285/318),2022年水质合格率为51.8%(101/195),不同年份合格率比较,差异有统计学意义(χ2=259.208,P<0.05)。浑浊度、大肠菌群两项指标合格率最高,其次是细菌总数、pH值和尿素,合格率最低的为游离性余氯。不同类型的游泳场所池水合格率最高的为学校附属泳池,其次为宾馆饭店附属泳池,社会性泳池合格率最低。结论2018—2022年影响海淀区游泳场所水质合格率的指标主要为游离性余氯和尿素,对游泳场所的日常监督管理特别是对社会性泳池的卫生监督工作需进一步加强,使游泳池水合格率得到进一步提高,保障游泳安全。

关键词：游泳场所水质卫生状况

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King cobra peptide OH-cath30 as a potential candidate drug through clinic drug-resistant isolates

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Zoological Research 2018年第2期39卷 87-96页

作者： Feng Zhao Xin-Qiang Lan yan Du Pei-Yi Chen Jiao Zhao Fang Zhao Wen-Hui Lee Yun Zhang Key Laboratory of Subtropical Medicinal Edible Resources Development and Utilization in Yunnan Province Department of Biology and Chemistry Puer University Puer Yunnan 665000 China Key Laboratory of Bioactive Peptides of Yunnan Province/Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences Kunming Institute of Zoology Kunming Yunnan 650223 China Department of Clinical Laboratories First Affiliated Hospital of Kunming Medical University Kunming Yunnan 650032 China Institute of Comparative Study of Traditional Materia Medica Institute of Integrative Medicine of Fudan University Shanghai 200433 China

Cationic antimicrobial peptides （AMPs） are considered as important candidate therapeutic agents, which exert potent microbicidal properties against bacteria, fungi and some viruses. Based on our previous findings king cobra cathelicidin （OH-cath） is a 34-amino acid peptide that exerts strong antibacterial and weak hemolytic activity. The aim of this research is to evaluate the efficacy of both OH-cath30 and its analog D-OH-cath30 against clinical isolates comparing with routinely utilized antibiotics in vitro. In this study, 584 clinical isolates were tested （spanning 2013-2016） and the efficacy of the candidate peptides and antibiotics were determined by a broth microdilution method according to the CLSI guidelines. Among the 584 clinical isolates, 85% were susceptible to OH-cath30 and its analogs. Both L- and D-OH-cath30 showed higher efficacy against （toward） Gram-positive bacteria and stronger antibacterial activity against nearly all Gram-negative bacteria tested compare with antibiotics. The highest bactericidal activity was detected against Acinetobacter spp., including multi-drug-resistant Acinetobacter baumannfi （MRAB） and methicillin-resistant Staphylococcus aureus （MRSA）. The overall efficacy of OH-cath30 and its analogs was higher than that of the 9 routinely used antibiotics. OH-cath30 is a promising candidate drug for the treatment of a wide variety of bacterial infections which are resistant to many routinely used antimicrobial agents.

关键词： cathelicidin Antibacterial agent Clinicalisolates OH-cath30

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Circulating circRNA expression profile and its potential role in late recurrence of paroxysmal atrial fibrillation post catheter ablation

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Journal of Geriatric Cardiology 2023年第11期20卷 788-800页

作者： Shan-Shan LIU Hong-yang GUO Jian ZHU Jin-Ling MA Sai-Zhe LIU Kun-Lun HE Su-yan BIAN Department of Cardiology the Second Medical CenterNational Clinical Research Center of Geriatric DiseaseBeijing Key Laboratory of Chronic Heart Failure Precision MedicineChinese PLA General HospitalBeijingChina Department of Cardiology the Six Medical CenterChinese PLA General HospitalBeijingChina Medical Big Data Research Center Medical Innovation Research DepartmentBeijing Key Laboratory of Chronic Heart Failure Precision MedicineChinese PLA General HospitalBeijingChina

BACKGROUND catheter-based pulmonary vein isolation(PVI) is an effective and well-established intervention for symptomatic paroxysmal atrial fibrillation(PAF). Nevertheless, late recurrences of atrial fibrillation(LRAF) occurring during 3 to 12months are common, and the underlying mechanisms remain elusive. Circular RNAs(circ RNAs) in atrial tissue have been linked to the pathophysiological mechanisms and progression of PAF in a few studies. However, their expression patterns in peripheral blood and regulatory function in LRAF are not *** In the present study, the expression profile of circulating circ RNAs in three paired nonvalvular PAF patients with or without LRAF was investigated by high-throughput sequencing and validated by quantitative real-time polymerase chain reaction(q RT-PCR). Bioinformatics analyses, including Gene Ontology(GO), Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway analysis, and circ RNA/mi RNA regulatory network, were performed to predict the functions and potential regulatory roles of differentially expressed(DE) circ *** A total of 12,834 circ RNAs, comprising 5,491 down-regulated and 7,343 up-regulated circ RNAs, were found to be DE in blood smaples from the two groups in peripheral blood between LRAF and non-recurrence control individuals. The most enriched GO categories in terms of molecular function, biological process, and cellular component features were catalytic activity,cellular metabolic process, and intracellular part, respectively. The KEGG enrichment study revealed that the most important metabolic process controlled by DE circ RNAs is endocytosis. In the circ RNA/micro RNAs interaction network, four up-regulated circ RNAs(hsa_circ_0002665, hsa_circ_0001953, hsa_circ_0003831, and hsa_circ_0040533) and one down-regulated circ RNA(hsa_circ_0041103) were predicted to play potential regulatory roles in the pathogenesis of *** This investigation discovered the expression pattern of circulating c

关键词： atrial fibrillation paroxysmal

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Identification and characterization of two novel cathelicidins from the frog Odorrana livida

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Zoological Research 2019年第2期40卷 94-101页

作者： Ruo-Han Qi yan Chen Zhi-Lai Guo Fen Zhang Zheng Fang Kai Huang Hai-Ning Yu Yi-Peng Wang College of Pharmaceutical Sciences Soochow University School of Biology & Basic Medical Sciences Medical College Soochow University Department of Bioscience and Biotechnology Dalian University of Technology

Antimicrobial peptides(AMPs) are a group of gene-encoded small peptides that play pivotal roles in the host immune system of multicellular *** are an important family of AMPs that exclusively exist in vertebrates. Many cathelicidins have been identified from mammals, birds, reptiles and fish. To date, however, cathelicidins from amphibians are poorly understood. In the present study, two novel cathelicidins(OL-cath1 and 2) were identified and studied from the odorous frog Odorrana ***, the cDNAs encoding the OL-caths(780 and735 bp in length, respectively) were successfully cloned from a lung cDNA library constructed for the frog. Multi-sequence alignment was carried out to analyze differences between the precursors of the OL-caths and other representative *** peptide sequences of OL-cath1 and 2 were predicted(33 amino acid residues) and their secondary structures were determined(OL-cath1 showed a random-coil conformation and OL-cath2 demonstrated α-helical conformation). Furthermore,OL-cath1 and 2 were chemically synthesized and their in vitro functions were determined. Antimicrobial and bacterial killing kinetic analyses indicated that OL-cath2 demonstrated relatively moderate and rapid antimicrobial potency and exhibited strong anti-inflammatory activity. At very low concentrations(10 μg/mL), OL-cath2 significantly inhibited the lipopolysaccharide(LPS)-induced transcription and production of pro-inflammatory cytokines TNF-α, IL-1βand IL-6 in mouse peritoneal macrophages. In contrast, OL-cath1 did not exhibit any detectableantimicrobial or anti-inflammatory activities. Overall,identification of these OL-caths from O. livida enriches our understanding of the functions of cathelicidins in the amphibian immune system. The potent antimicrobial and anti-inflammatory activities of OL-cath2 highlight its potential as a novel candidate in anti-infective drug development.

关键词： Antimicrobial peptides (AMPs) cathelicidins Odorrana livida OL-caths Antimicrobial activity Anti-inflammatory activity

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液液萃取--超高效液相色谱串联质谱法同时测定尿中11种羟基多环芳烃

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环境卫生学杂志 2024年第1期14卷 72-78页

作者：谭磊邓芬芳卢祝靓子罗晓燕潘心红广州市疾病预防控制中心毒理与生化检验部广州510440

目的建立可同时测定尿中11种羟基多环芳烃(hydroxy-polycyclic aromatic hydrocarbons,OH-PAHs)的超高效液相色谱串联质谱分析(ultra-high performance liquid chromatography tandem with mass spectrometry,UPLC-MS/MS)法。方法尿液样品在pH=5.5条件下,在37℃水浴中经β-葡萄糖苷酸酶—芳基硫酸酯酶避光水解16 h,3.0 mL正己烷萃取、涡旋、离心,取上层有机相,重复萃取两次,合并萃取液,氮吹至近干,再复溶于20%乙腈水溶液中。流动相为水和10%异丙醇甲醇溶液,梯度洗脱模式洗脱,CORTECS C18色谱柱分离,ESI-模式测定尿液中11种羟基多环芳烃浓度,内标法定量分析。结果11种羟基多环芳烃线性关系良好,相关系数(r)均大于0.997。方法的检出限为0.01~0.10 ng/mL;日间回收率为79.8%~97.3%,精密度为1.9%~4.7%(n=3);日内回收率为78.6%~92.3%,精密度为3.7%~7.1%(n=3)。结论该方灵敏度高,准确可靠,适用于人群中多种羟基多环芳烃的监测。

关键词：超高效液相色谱串联质谱羟基多环芳烃液液萃取尿液

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China's Raising Industrial Base for Micro-Satellite and Their Applications——Aerospace Dongfanghong Development Ltd. Shenzhen

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Aerospace China 2015年第3期16卷 36-41页

作者： JIANG yan ZHANG Di Shenzhen Dongfanghong

Aerospace Dongfanghong development Ltd. Shenzhen （Shenzhen Dongfanghong） subsidiary of China Academy of Space Technology （CAST）, CASC, was founded in Octo- ber 2008. As a microsatellite system design and integration provider of CASC, Shenzhen Dongfanghong has the ability to design, development, assembly, integrate and test micro- satellites. In order to adapt to the rapid developing micro- satellite market, Shenzhen Dongfanghong is exploring and practicing a commercialized satellite operation mode. It has developed a series of products, applying space technology in the areas of high-altitude meteorological detection and ma- rine environmental monitoring.

关键词：中国空间技术研究院航天科技集团东方红深圳卫星产业应用基础海洋环境监测

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