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Diversity of the Formicidae (Hymenoptera) carrion communities in Lisbon (Portugal): preliminary approach as seasonal and geographic indicators

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Forensic Sciences Research 2018年第1期3卷 65-73页

作者： António Neto-Silva Ricardo Jorge Dinis-Oliveira catarina prado e castro Department of Public Health Forensic Sciences and Medical EducationFaculty of MedicineUniversity of PortoPortoPortugal Department of Sciences IINFACTS-Institute of Research and Advanced Training in Health Sciences and TechnologiesUniversity Institute of Health Sciences(IUCS)CESPUCRLGandraPortugal UCIBIO REQUIMTE-Laboratory of ToxicologyDepartment of Biological SciencesFaculty of PharmacyUniversity of PortoPortoPortugal Department of Life Sciences Centre for Functional EcologyUniversity of CoimbraCoimbraPortugal

The value of the Formicidae (Insecta,Hymenoptera) community in forensic investigations is poorly studied in *** order to better understand the structure and dynamics of this group of insects in cadavers and their putative value in forensic investigations,studies were carried out in Lisbon area during one *** carcasses were used as a model of human *** entomofauna attracted to the carcasses was collected over a period of about 2 months in each season of the *** collection of ants was performed at regular intervals,daily in the first 23 days and then with intervals of 2,3 or 5 days until the end of the *** stages of cadaveric decomposition were recognized with the ants being present in all of *** hundred and nine specimens were captured: 7 in autumn,6 in winter,90 in spring and 206 in *** specimens belong to three subfamilies and seven different *** of them had never been mentioned before as being associated to cadavers in this geographical *** and summer had the greater diversity of species and a larger number of *** luteus was the dominant species in spring and Tapinoma nigerrimum in *** the information of other similar studies carried out in the Iberian Peninsula,the sarcosaprophagous Formicidae community found in Lisbon is unique and different from other studied locations,which supports the need to perform regional *** results shown that ants do not present a definite pattern of succession,but some species have the potential to be seasonal or geographic indicators.

关键词： Forensic science forensic entomology Formicidae sarcosaprophagous community decomposition process postmortem interval seasonal study

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effect of resistance training volume on body adiposity,metabolic risk,and inflammation in postmenopausal and older females:Systematic review and meta-analysis of randomized controlled trials

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Journal of Sport and Health Science 2024年第2期13卷 145-159页

作者： Paulo Ricardo prado Nunes Pamela castro-e-Souza Anselmo Alves de Oliveira Bruno de Freitas Camilo Gislaine Cristina-Souza Lucio Marques Vieira-Souza Marcelo Augusto da Silva Carneiro Department of Body and Human Movement Minas Gerais State University(UEMG)37902-092 PassosMGBrazil University Center of Planalto de Araxa(UNIARAXA) 38180-129 AraxaMGBrazil Physical Activity Health and Ageing Research Group(GPASE)Minas Gerais State University(UEMG)37902-092 PassosMGBrazil exercise and Nutrition Research Group(GPeN) Minas Gerais State University(UEMG)37902-092 PassosMGBrazil Sport Sciences and Physical exercise Study and Research Group(GePCeeX) Minas Gerais State University(UEMG)37902-092 PassosMGBrazil Metabolism Nutrition and Exercise LaboratoryPhysical Education and Sport CenterLondrina State University(UEL)86050-070 LondrinaPRBrazil Postgraduate Program in Physical education Federal University of Sergipe(UFS)49107-230 Sao CristovaoSEBrazil

Purpose:This meta-analytical study aimed to explore the effects of resistance training(RT) volume on body adiposity,metabolic risk,and inflammation in postmenopausal and older ***:A systematic search was performed for randomized controlled trials in PubMed,Scopus,Web of Science,and *** controlled trials with postmenopausal and older females that compared RT effects on body adiposity,metabolic risk,and inflammation with a control group(CG) were *** reviewers selected the studies,extracted the data,and performed the risk of bias and certainty of the evidence(Grading of Recommendations,Assessment,Development,and evaluation(GRADe)) *** body and abdominal adiposity,blood lipids,glucose,and C-reactive protein were included for meta-analysis.A random-effects model,standardized mean difference(Hedges’ g),and 95% confidence interval(95%CI) were used for ***:Twenty randomized controlled trials(overall risk of bias:some concerns;GRADe:low to very low) with overweight/obese postmenopausal and older females were *** groups were divided into low-volume RT(LVRT,~44 sets/week) and high-volume RT(HVRT,~77 sets/week).Both RT groups presented improved body adiposity,metabolic risk,and inflammation when compared to ***,HVRT demonstrated higher effect sizes than LVRT for glucose(HVRT=-1.19;95%CI:-1.63 to-0.74;LVRT=-0.78;95%CI:-1.15 to-0.41) and C-reactive protein(HVRT=-1.00;95%CI:-1.32 to-0.67;LVRT=-0.34;95%CI,-0.63 to-0.04)) when compared to ***:Compared to CG,HVRT protocols elicit greater improvements in metabolic risk and inflammation outcomes than LVRT in overweight/obese postmenopausal and older females.

关键词： C-reactive protein Fat mass Lipid profile Menopause Strength training

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Targeting of Pollen Tubes to Ovules Is Dependent on Nitric Oxide （NO） Signaling

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Molecular Plant 2008年第4期1卷 703-714页

作者： Ana Margarida prado Renato Colaco Nuno Moreno Ana catarina Silva Jose A. Feijo Instituto Gulbenkian de Ciencla Centro de Blologia do Desenvolvimento PT-2780-156 Oeiras Portugal Universidade de Lisboa Faculdade de Clencias Dept Biologia Vegetal Campo Grande Ed.C2. PT-1749-016 Lisboa Portugal

The guidance signals that drive pollen tube navigation inside the pistil and micropyle targeting are still, to a great extent, unknown. Previous studies in vitro showed that nitric oxide （NO） works as a negative chemotropic cue for pollen tube growth in lily （Lilium Iongiflorum）. Furthermore, Arabidopsis thaliana Atnosl mutant plants, which show de- fective NO production, have reduced fertility. Here, we focus in the role of NO in the process of pollen-pistil communication, using Arabidopsis in-vivo and lily semi-vivo assays. Cross-pollination between wild-type and Atnosl plants shows that the mutation affects the pistil tissues in a way that is compatible with abnormal pollen tube guidance. Moreover, DAF- 2DA staining for NO in kanadi floral mutants showed the presence of NO in an asymmetric restricted area around the micropyle. The pollen-pistil interaction transcriptome indicates a time-course-specific modulation of transcripts of AtNOS1 and two Nitrate Reductases （nrl and nr2）, which collectively are thought to trigger a putative NO signaling pathway. Semivivo assays with isolated ovules and lily pollen further showed that NO is necessary for micropyle targeting to occur. This evidence is supported by CPTIO treatment with subsequent formation of balloon tips in pollen tubes facing ovules. Activation of calcium influx in pollen tubes partially rescued normal pollen tube morphology, suggesting that this pathway is also dependent on Ca^2＋ signaling. A role of NO in modulating Ca^2＋ signaling was further substantiated by direct imaging the cytosolic free Ca^2＋ concentration during NO-induced re-orientation, where two peaks of Ca^2＋ occur--one during the slowdown/stop response, the second during re-orientation and growth resumption. Taken together, these results provide evidence for the participation of NO signaling events during pollen-pistil interaction. Of special relevance, NO seems to directly affect the targeting of pollen tubes to the ovule＇s micropyle by modu

关键词： pollen tubes NO fertilization guidance calcium signaling

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Unsuitable use of spin and pseudospin symmetries with a pseudoscalar Cornell potential

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Chinese Physics B 2014年第9期23卷 60-61页

作者： L.B.castro A.S.de castro Departamento de Física CFM Universidade Federal de Santa Catarina Departamento de Física Universidade Federal do Maranho Campus Universtitrio do Bacanga Departamento de Física e Química Campus de Guaratinguet Universidade Estadual Paulista

The concepts of spin and pseudospin symmetries has been used as mere rhetorics to decorate the pseudoscalar potential [Chin. Phys. B 22 090301 （2013）]. It is also pointed out that a more complete analysis of the bou... 详细信息

关键词： Dirac equation pseudoscalar Comell potential spin symmetry pseudospin symmetry

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Network of GRAS transcription factors in plant development,fruit ripening and stress responses

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Horticulture Research 2023年第12期10卷 286-303页

作者： catarina Neves Beatriz Ribeiro Rute Amaro Jesús expósito Jérôme Grimplet Ana Margarida Fortes BioISI-Biosystems and Integrative Sciences Institute Faculty of SciencesUniversity of LisbonCampo Grande1749-016 LisboaPortugal Centro de Investigación y Tecnología Agroalimentaria de Aragón(CITA) Departamento de Ciencia VegetalGobierno de AragónAvda.Montañana 93050059 ZaragozaSpain Instituto Agroalimentario de Aragón-IA2(CITA-Universidad de Zaragoza) Calle Miguel Servet 17750013 ZaragozaSpain

The plant-specific family of GRAS transcription factors has been wide implicated in the regulation of transcriptional reprogramming associated with a diversity of biological functions ranging from plant development processes to stress *** analyses of GRAS transcription factors supported by in silico structural and comparative analyses are emerging and clarifying the regulatory networks associated with their biological *** this review,a detailed analysis of GRAS proteins’structure and biochemical features as revealed by recent discoveries indicated how these characteristics may impact subcellular location,molecular mechanisms,and *** issues associated with GRAS classification into different subfamilies in diverse plant species even in the presence of robust genomic resources are discussed,in particular how it affects assumptions of biological *** into the mechanisms driving evolution of this gene family and how genetic and epigenetic regulation of GRAS contributes to subfunctionalization are ***,this review debates challenges and future perspectives on the application of this complex but promising gene family for crop improvement to cope with challenges of environmental transition.

关键词： function GRAS plant

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Water availability drives gradients of tree diversity,structure and functional traits in the Atlantic-Cerrado-Caatinga transition,Brazil

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Journal of Plant ecology 2018年第6期11卷 803-814页

作者： Marcela de castro Nunes Santos Terra Rubens Manoel dos Santos Jamir Afonso do prado Júnior José Marcio de Mello José Roberto Soares Scolforo Marco Aurélio Leite Fontes Ivan Schiavini Aliny Aparecida dos Reis Inacio Thomaz Bueno Luiz Fernando Silva Magnago Hans ter Steege Departamento de Ciências Florestais Universidade Federal de LavrasCampus UniversitárioPO Box 3037LavrasMinas Gerais 37200-000Brazil Instituto de Biologia Universidade Federal de UberlândiaCampus Umuarama-Bloco 2DPO Box 593UberlândiaMinas Gerais 38400-902Brazil Applied ecology and Conservation Lab Programa de Pós‐graduação Ecologia e Conservação da BiodiversidadeUniversidade Estadual de Santa CruzIlhéusBahia 45662-900Brazil Naturalis Biodiversity Center Vondellaan 55Postbus 95172300 RA LeidenThe Netherlands Coordenação de Botânica Museu Paraense Emílio GoeldiAv.Magalhães Barata 376PO Box 399BelémPara´66040-170Brazil

Aims Climate and soil are among the most important factors determin-ing variation in tree communities,but their effects have not been thoroughly elucidated to date for many vegetation *** this study,we evaluate how climate and soil gradients affect gradients of vegetation composition,species diversity and dominance,structure and functional traits(seed mass and wood density)using over 327000 trees in 158 sites distributed along environmental gradients in the transitions among the Atlantic forest,Cerrado and Caatinga in Minas Gerais State(MG),Brazil(nearly 600000 km2).Methods Gradients in species,genus and family abundance in addition to basal area,stem density,species diversity(Fisher’s alpha),domin-ance percentage,seed mass and wood density were correlated using multiple regressions with environmental variables,as summarized in four principal component analysis axes(two climatic-precipi-tation seasonality and temperature range-and two edaphic-soil fertility and soil moisture).Additionally,ordinary kriging maps were used to better illustrate the *** Findings Multiple regression models indicate that all variables but domin-ance percentage were affected by one or more of the environmental gradients,but the average R2 was low(26.25%).Kriging maps rein-forced the patterns observed in the regression *** seasonality and soil moisture gradients were the most important gra-dients affecting vegetation *** finding suggests that water availability is an important determinant of vegetation features in these vegetation transitions.

关键词： climate environmental gradient soil southeastern Brazil vegetation

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青少年和年轻成人先天性心脏病的心理社会远期转归

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中国当代儿科杂志 2013年第10期15卷 810-816页

作者： Maria emlha Gulmaraes Arelas catarina I. Pinto Patricia F. Vieira Flavio Teixeira Rosalia. Coelho Isabela Freitas Samantha Matos Marta castro Sofia Sarmento Victor Viana Jorge Quintas Jose C. Areias Department of Psychology Instituto Superior de Ciencias da Saude do Norte (CESPU) Gandra Portugal CINeICC Centro de Investigaao do Nucleo de Estudos e Intervencao Cognitivo-Comportamental Coimbra Portugal UNIPSA Unidade de Investigacao de Psicologia e Saude Gandra Portugal Department of Paediatrics (Cardiology) Hospital Sao Joao Porto Medical School University of Porto Portugal Faculty of Nutrition University of Porto Portugal Faculty of Law University of Porto Portugal Unidade de Investigacao Cardiovascular University of Porto Portugal

目的先天性心脏病(CHD)是一种在全球范围内高发的慢性疾病,通常在出生时或出生前确诊。由于诊断以及早期药物和手术治疗的条件较好,患者的生存率达到90%,并且生存时间不断延长,面临着生活中的各种挑战。该研究探讨了不同人口统计学、临... 详细信息

目的先天性心脏病(CHD)是一种在全球范围内高发的慢性疾病,通常在出生时或出生前确诊。由于诊断以及早期药物和手术治疗的条件较好,患者的生存率达到90%,并且生存时间不断延长,面临着生活中的各种挑战。该研究探讨了不同人口统计学、临床和心理社会变量对CHD青少年和年轻成人患者的生活质量(QOL)感知、心理社会适应和精神障碍患病率的影响,评估CHD青少年和年轻成人患者的生活质量、心理社会适应和精神障碍发病率,确定哪些变量(人口统计学、临床和心理社会变量)对缓解压力、促进恢复起到积极作用,哪些变量起到不良作用。方法该研究入组了150名CHD患者(男87例,女63例),年龄12～26岁(平均年龄:17.45±3.373岁)。对参与者进行了关于社会支持、家庭教育方式、自我形象、人口统计信息和生理限制等方面的访谈。他们对标准化精神病学访谈(SADS-L)中的问题进行了回答,并填写了自我报告问卷,以进行生活质量(WHOQOL-BReF)和心理社会适应(YSR/ASR)的评估。结果所有参与者精神障碍的终身患病率为18.7%(女性25.4%,男性13.8%)。57.1%的参与者有过留级(平均1.53±0.804年)。与整体葡萄牙人相比,CHD患者的生活质量感知在社会关系、环境、身体和总体方面较好。而女性、学习成绩差、较少的社会支持、复杂性CHD、紫绀型CHD、中重度残留病灶、接受过手术和有生理限制患者生活质量感知较差。除了紫绀,所有这些变量也会引起较差的心理社会适应。结论女性CHD患者及学习成绩差且获得社会支持少的CHD患者的心理社会适应和生活质量较差。

关键词：先天性心脏病风险因素生活质量心理社会适应精神障碍患病率

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In vitro induced pluripotency from urine-derived cells in porcine

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World Journal of Stem Cells 2022年第3期14卷 231-244页

作者： Kaiana Recchia Lucas Simões Machado Ramon Cesar Botigelli Naira Caroline Godoy Pieri Gabriela Barbosa Raquel Vasconcelos Guimarães de castro Mariana Groke Marques Laís Vicari de Figueiredo Pessôa Paulo Fantinato Neto Flávio Vieira Meirelles Aline Fernanda de Souza Simone Maria Massami Kitamura Martins Fabiana Fernandes Bressan Department of Surgery Faculty of Veterinary Medicine and Animal SciencesUniversity of São PauloPirassununga 13635-000São PauloBrazil Department of Pharmacology and Biotechnology Institute of BioscienceSão Paulo State UniversityBotucatu 18618-689São PauloBrazil Department of Veterinary Medicine Faculty of Animal Sciences and Food EngineeringUniversity of São PauloPirassununga 13635-000São PauloBrazil embrapa Suínos e Aves Empresa Brasileira de Pesquisa AgropecuáriaConcordia 89715-899Santa CatarinaBrazil Department of Animal Sciences Faculty of Animal Sciences and Food EngineeringUniversity of São PauloPirassununga 13635-000São PauloBrazil

BACKGROUND The generation of induced pluripotent stem cells(iPSC)has been a game-changer in translational and regenerative medicine;however,their large-scale applicability is still hampered by the scarcity of accessible,safe,and reproducible *** porcine model is a large biomedical model that enables translational applications,including gene editing,long term in vivo and offspring analysis;therefore,suitable for both medicine and animal *** To reprogramme in vitro into pluripotency,and herein urine-derived cells(UDCs)were isolated from porcine *** The UDCs were reprogrammed in vitro using human or murine octamer-binding transcription factor 4(OCT4),SRY-box2(SOX2),Kruppel-like factor 4(KLF4),and C-MYC,and cultured with basic fibroblast growth factor(bFGF)*** characterize the putative porcine iPSCs three clonal lineages were submitted to immunocytochemistry for alkaline phosphatase(AP),OCT4,SOX2,NANOG,TRA181 and SSeA 1 *** transcripts related to the pluripotency(OCT4,SOX2 and NANOG)were analyzed via reverse transcription quantitative realtime polymerase chain reaction in different time points during the culture,and all three lineages formed embryoid bodies(eBs)when cultured in suspension without bFGF *** The UDCs were isolated from swine urine samples and when at passage 2 submitted to in vitro *** of putative iPSCs were obtained only from UDCs transduced with the murine factors(mOSKM),but not from human factors(hOSKM).Three clonal lineages were isolated and further cultured for at least 28 passages,all the lineages were positive for AP detection,the OCT4,SOX2,NANOG markers,albeit the immunocytochemical analysis also revealed heterogeneous phenotypic profiles among lineages and passages for NANOG and SSeA1,similar results were observed in the abundance of the endogenous transcripts related to pluripotent *** the clonal lineages when cultured in suspension without

关键词： Induced pluripotent stem cells Noninvasive Pluripotency Reprogramming Urine Porcine

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Just in Time education: educational Technology for Professional’s Health

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Open Journal of Nursing 2022年第5期12卷 349-362页

作者： Monica Motta Lino Jean Carlos Müller Da Silva Bizarro† eloisa Cesa Zuanazzi† Luiza Sheyla evenni Porfírio Will castro† Felipa Rafaela Amadigi Thaise Torres Sophia Da Fonseca Rangel Interdisciplinary Laboratory of educational Technologies in Health Department of Nursing Federal University of Santa Catarina Florianópolis Brazil

Objective: Verify if the educational technology based on Just in Time education is relevant to Permanent Health education in Workers’ Health. Method: This study is exploratory and descriptive qualitative research. Were performed semi-structured interviews were carried out with the worker’s health team of a large company from the South of Brazil region. The collected data were carried out in 2021, between January and May, through the virtual environment, with eight participants from the Work Health Team of eletrosul, located in Florianópolis, who agreed to participate in the research by signing the Free and Informed Consent Term, in accordance with the data saturation criterion. The research was approved by the ethics Committee (n. 4079133) and the data were submitted to Bardin for thematic content analysis. Result: The results are presented from three categories: 1) expansion and inclusion in Permanent education needs;2) Tools adopted in educational processes;3) Just in Time education: educational technology for workers’ health. Conclusion: The active methodology Just in Time education helps the processes of Permanent education in Health, which moved from a face-to-face model to e-learning and required the knowledge renewal in the technology area to maintain efficient communication. This methodology allows quick and accurate access to information from a reliable, adequate, and up-to-date source, causing a positive impact on the qualified and problem-solving work process, in line with the needs of the employer and the company. Therefore, Just in Time education can provide standardization, organization, and informational and operational quality necessary in this context, in addition to serving as an indicator and data generator (management tool).

关键词： Permanent Health education educational Technology Worker’s Health Active Methodology Qualitative Research

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effect of a Diet Rich in Interesterified, Non-Interesterified and Trans Fats on Biochemical Parameters and Oxidative Status of Balb-c Mice

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Food and Nutrition Sciences 2020年第11期11卷 1032-1052页

作者： elinete eliete de Lima Luiza S. e. P. W. castro Valdelúcia Maria A. de S. Grinevicius Josiane Hilbig Nádia S. R. S. Mota Rodrigo C. Zeferino Roberta C. da Silva Iván Jachmanián edson Luiz da Silva Rozangela C. Pedrosa Jane Mara Block Laboratory of Fats and Oils Department of Food Science and Technology Federal University of Santa Catarina Florianópolis SC Brazil Campus Florianópolis Continente Federal Institute of Education Science and Technology of Santa Catarina Florianópolis-SC Brazil Laboratory of experimental Biochemistry Department of Biochemistry Federal University of Santa Catarina Florianópolis SC Brazil Faculty of Pharmaceutical Sciences University of S&#227 o Paulo S&#227 o Paulo-SP Brazil Laboratory of Lipid Research Department of Family and Consumer Sciences College of Agriculture and Environmental Sciences North Carolina A & T State University NC USA Department of Food Science and Technology Facultad de Química Universidad de la República Montevideo Uruguay Laboratory of Lipids Department of Clinical Analysis Federal University of Santa Catarina Florianópolis-SC Brazil

The aim of this study was to investigate the effects of different modified fats on the body weight, biochemical profile, and biomarkers of hepatic oxidative status in Balb-c mice. The animals were divided into four groups and fed for 75 days with a normolipidic (Control Group, CG) or hiperlipidic diets (40% kcal) containing a commercial interesterified fat (IFG) rich in palmitic acid (39%);a blend of non-interesterified fat (NIFG), with 2-fold less saturated fatty acids at the sn-2 position of triacylglycerols;or a partially hydrogenated vegetable oil (PHFG), source of trans fatty acid (20%) and of linolenic acid (6%). The mice of the IFG and NIFG presented similar results in all evaluated parameters. The serum biochemical profile and hepatic oxidative stress markers in mice of the PHFG were similar to CG, except for total cholesterol (TC) which was significantly higher (p e="font-family:Verdana;">e PHFG. The mice feed with interesterified fat (IFG) showed serum TC (p < 0.01), non-HDL-C (p < 0.05), gluco

关键词： Interesterified Fat Trans Fat Palmitic Acid Cholesterol Oxidative Stress

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