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Genetic variability of Rhizoctonia solani Kühn and its resistance to fungicides

Studies in Fungi 2020年第1期5卷 381-391页

作者： castillo rf Gallegos G Flores OA Aguilar GC Rodriguez HR Hernandez CFD Saltillo Experimental Station Instituto Nacional de Investigaciones Forestales Agrícolas y Pecuarias.Carr.Saltillo-Zacatecas km 342+119No.9515Col.ExHacienda de BuenavistaSaltilloCoah.C.P.25315 Department of Agricultural Parasitology Universidad Autónoma Agraria Antonio NarroBuenavista25315SaltilloCoahuilaMéxico Food Research Department School of Chemical SciencesUniversidad Autónoma de Coahuila25000SaltilloCoahuilaMexico

The aims of this study are to determine the population genetic structure of Rhizoctonia solani,associated with potato,and to assess its resistance degree to pencycuron and thiabendazole fungicides.A total of 57 strains of *** were isolated from different potato crop fields in Mexico,and were classified according to their anastomosis group(AG)using the polymerase chain reaction(PCR)and restriction fragment length polymorphism(rfLP)-based ***,the amplified fragments were digested with four different endonucleases(Mse I,Ava II,Mun I and Hinc II).The Repetitive Element Sequence-Based PCR(rep-PCR)technique was used to determine the genetic diversity in *** *** obtained by PCR-rfLP showed that 81%of the isolates were identified as belonging to AG-3 group,14%to AG1-1B and 5%to AG-11 *** response to fungicides inhibition,IC_(50)between 0.014-0.039 mg a.i./L for pencycuron,and 0.82 to 2.91 for thiabendazole were determined,This IC_(50)value showed that the resistance factor(rf)values ranged from 1.4 to 3.945 and 0.27 to 0.97,respectively;this tendency suggests that isolates are susceptible to *** AG groups showed a heterogeneous resistance to pencycuron and thiabendazole,being more tolerant to these fungicides AG1-1B and AG-11 *** genetic analysis shows a low genetic diversity among(P-value 0.3225)and within(P-value 0.3275)populations;this can indicate a clonal reproduction and little parasexual cycle among the analyzed isolations.

关键词： Anastomosis group genetic diversity genetic resistance Mexico PCR rfLP parasexual cycle REP PCR Wright’s statistics

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