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Phenotypic and Antimicrobial Susceptibility Studies of Proteus mirabilis Isolates from Fresh Water Fishes in FCT, Abuja-Nigeria

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Advances in Microbiology 2023年第5期13卷 249-265页

作者： Victor Bitrus Shammah Samuel Mailafia James Agbo Ameh casmir ifeanyichukwu cejetan ifeanyi Adetola Opeyemi Adebari Ebenezer Odey Odey Reece Asoloko Sabo Department of Veterinary Microbiology Faculty of Veterinary Medicine University of Abuja Abuja Nigeria Department of Veterinary Public Health and Preventive Medicine Faculty of Veterinary Medicine University of Abuja Abuja Nigeria Department of Molecular Biotechnology Jagiellian University Krakow Poland

Our study was carried out to determine the phenotypic characterization antimicrobial susceptibility of Proteus mirabilis from fish in FCT, Abuja using isolation, selective plating, preliminary observation, complete biochemical method and antimicrobial susceptibility testing. The biochemical tests conducted includes include Citrate Utilization test, Triple Sugar Iron test, Urea test, Methyl Red test, Indole test, and Voges Proskauer test. The isolates were confirmed by MicrobactTMGNB24E identification kit (Oxiod, UK). A total of 400 fish samples were bought in the market from three area council of the FCT. The result of the study showed overall prevalence rate of (13) 3.25% of Proteus mirabilis isolates. Distribution based on Area councils showed that AMAC had higher prevalence rate of 4.81%, while Bwari had 2.99% and Gwagwalada with 2.57% prevalence. All isolates were subjected to antimicrobial susceptibility testing using the modified single disc diffusion method. From the antimicrobial susceptibility testing done it was discovered that Proteus mirabilis are resistant to Amoxyclav (100%), Erythromycin (92.3%), Tetracycline (92.3%) and Ceftriaxone (23.1%). However, the isolates were susceptible to Ofloxacin (100%), Netillin (92.3%), Levofloxacin (92.3%), Ceftazidime (76.9%), Co-trimoxazole (69.2%) and Gentamicin (61.5%). Since Proteus mirabilis sources of zoonotic diseases and can potentially be dangerous to humans and other animals, our research was able to isolate it from fresh water fish sold in the Federal Capital Territory. This makes public health awareness of the risks associated with Proteus mirabilis in Nigeria necessary.

关键词： Proteus mirabilis Fishes Isolation MicrobactTMGNB24E Biochemical Characterization Prevalence

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Prevalence and Antimicrobial Resistance of Gram-Negative Bacteria Isolates in Shellfish Samples from Two River Estuaries in South-South Nigeria

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Advances in Microbiology 2021年第9期11卷 428-443页

作者： Nsikan Samuel Udoekong Bassey Enya Bassey Anne Ebri Asuquo Otobong Donald Akan casmir ifeanyichukwu Cajetan ifeanyi Science Technology Department Akwa Ibom State Polytechnic Ikot Ekpene Nigeria Nigeria Country Office World Health Organization (WHO) Abuja Nigeria Department of Medical Laboratory Science University of Calabar Calabar Nigeria College of Food Science and Engineering Central South University of Forestry and Technology Changsha China Microbiology Department Akwa-Ibom State University Ikot Akpaden Nigeria Department of Veterinary Microbiology Faculty of Veterinary Medicine University of Abuja Abuja Nigeria

Antibiotic resistant bacteria pathogens remain the leading cause of shellfish borne diseases and a major health threat to humans worldwide. The objectives of this study were to isolate, identify, and determine the antibiotic resistance patterns of Gram-negative bacteria from shellfish. We analyzed a total of 540 shellfish (117 clams, 88 oysters, and 136 periwinkles) samples collected from different vendors at Iko and Douglas Creeks in Akwa Ibom State, South-South Nigeria. Conventional cultural techniques, morphological, biochemical characteristics, and PCR amplification were used to identify the bacterial isolates. Antibiotic susceptibility tests (Kirby-Bauer disk diffusion method) and ESBL phenotype (disk) of the isolates were performed. One hundred and thirty-five (135) Gram-negative bacteria comprising 5 genera and 14 species were detected at a prevalence of: Alcaligenes faecalis TRB-7 38 (28.2%), Pseudomonas oryzihabitans strain KCB005 16 (11.9%), Paenalcaligenes retgerii strain B5 12 (8.9%) Pseudomonas aeruginosa JB2 10 (7.4%), Providencia stuartii DMC-28b 9 (6.7%), Alcaligenes species TLT151 8 (5.9%), Pseudomonas aeruginosa CIFRI DTSB1 7 (5.2%), Paenalcaligenes species UN24 7 (5.2%), Alcaligenes faecalis BT10 7 (5.2%), Vibrio species strain PrVy108 6 (4.4%), Pseudomonas xiamenensis C10-2 5 (3.7%), Providencia vemicola Bu15_38 4 (2.9%), Pseudomonas anguillisceptica 4029 3 (2.2%), and Pseudomonas aeruginosa N15-01092 3 (2.2%). All tested isolates showed various degrees of resistance to the thirteen antimicrobials evaluated. High levels of resistance (100%) to cefepime and imipenem were expressed by all isolates except the Providencia species. For the EBSL indicators, all isolates apart from Alcaligenes species were resistant (100%) to ceftriaxone. A

关键词： Shellfish Gram-Negative Bacteria ESBL-Indicators Multi-Drug Resistance Calabar

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