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Dihydromyricetin and Salvianolic acid B inhibit alpha-synuclein aggregation and enhance chaperone-mediated autophagy

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Translational Neurodegeneration 2019年第1期8卷 215-229页

作者： Jia-Zhen Wu mustafa Ardah caroline Haikal Alexander Svanbergsson meike Diepenbroek Nishant N.Vaikath Wen Li Zhan-You Wang Tiago F.Outeiro Omar m.El-Agnaf Jia-Yi Li Institute of Neuroscience College of Life and Health SciencesNortheastern UniversityShenyangLiaoningChina Department of Biochemistry College of Medicine and Health SciencesUnited Arab Emirates UniversityPO Box 17666Al-AinUnited Arab Emirates Neural Plasticity and Repair Unit Wallenberg Neuroscience CenterDepartment of Experimental Medical ScienceBMC A1022184 LundSweden Neurological Disorders Research Center Qatar Biomedical Research Institute(QBRI)Hamad Bin Khalifa University(HBKU)Education CityQatar FoundationP.O.Box 5825DohaQatar Institute of Heath Sciences China Medical University110112 ShenyangPeople’s Republic of China Key Laboratory of maternal-Fetal medicine of Liaoning Province and Key Laboratory of Obstetrics and Gynecology of Higher Education of Liaoning Province Shengjing Hospital of China Medical UniversityShenyangChina Department of Experimental Neurodegeneration Center for Nanoscale Microscopy and Molecular Physiology of the BrainCenter for Biostructural Imaging of NeurodegenerationUniversity Medical Center Göttingen37073 GöttingenGermany max Planck Institute for Experimental medicine GöttingenGermany

Background:Progressive accumulation ofα-synuclein is a key step in the pathological development of Parkinson’s *** protein degradation and increased levels ofα-synuclein may trigger a pathological aggregation in vitro and in *** chaperone-mediated autophagy(CmA)pathway is involved in the intracellular degradation processes ofα-*** of the CmA pathway impairsα-synuclein degradation and causes ***:In the present study,we investigated the effects on the CmA pathway andα-synuclein aggregation using bioactive ingredients(Dihydromyricetin(DHm)and Salvianolic acid B(Sal B))extracted from natural medicinal *** both cell-free and cellular models ofα-synuclein aggregation,after administration of DHm and Sal B,we observed significant inhibition ofα-synuclein accumulation and *** were co-transfected with a Cterminal modifiedα-synuclein(SynT)and synphilin-1,and then treated with DHm(10μm)and Sal B(50μm)16 hours after transfection;levels ofα-synuclein aggregation decreased significantly(68%for DHm and 75%for Sal B).Concomitantly,we detected increased levels of LAmP-1(a marker of lysosomal homeostasis)and LAmP-2A(a key marker of CmA).Immunofluorescence analyses showed increased colocalization between LAmP-1 and LAmP-2A withα-synuclein inclusions after treatment with DHm and Sal *** also found increased levels of LAmP-1 and LAmP-2A both in vitro and in vivo,along with decreased levels ofα-***,DHm and Sal B treatments exhibited anti-inflammatory activities,preventing astroglia-and microglia-mediated neuroinflammation in BAC-α-syn-GFP transgenic ***:Our data indicate that DHm and Sal B are effective in modulatingα-synuclein accumulation and aggregate formation and augmenting activation of CmA,holding potential for the treatment of Parkinson’s disease.

关键词： chaperone-mediated autophagy macroautophagy alpha-synuclein protein aggregation Parkinson disease lysosomal-associated membrane protein

Asymptomatic Bacteriuria and Urinary Tract Infection in Pregnant Women with and without Diabetes mellitus and Gestational Diabetes mellitus—A Case-Control Study

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Journal of Diabetes mellitus 2017年第3期7卷 184-194页

作者： caroline Schneeberger Suzanne E. Geerlings Jan Jaap H.m. Erwich Edwin R. Van Den Heuvel Philippa middleton caroline A. Crowther Department of medical microbiology Academic Medical Center (AMC) University of Amsterdam Amsterdam The Netherlands Australian Research Centre for Health of Women and Babies Robinson Institute (ARCH) University of Adelaide Adelaide Australia Department of Internal medicine division of Infectious Diseases Academic Medical Center (AMC) University of Amsterdam Amsterdam The Netherlands Department of Obstetrics and Gynaecology University Medical Center Groningen (UMCG) University of Groningen Groningen The Netherlands Department of mathematics and Computer Science Technical University Eindhoven Eindhoven The Netherlands The Liggins Institute University of Auckland Auckland New Zealand

Background: Asymptomatic bacteriuria (ASB) and urinary tract infections (UTI) during pregnancy may contribute to adverse pregnancy outcomes. Diabetes mellitus (Dm) and gestational diabetes mellitus (GDm) are considered to be important additional risk factor for ASB and UTI during pregnancy. Aims: To investigate differences in prevalence of ASB and incidence of UTI in pregnant women with and without Dm and GDm to inform ASB screening and treatment policies. methods: Data from 214 pregnant women who gave birth during 2010 at the Women’s and Children’s Hospital, Adelaide, Australia where cases were women with a clinical diagnosis of (G)Dm and controls were matched on date of birth. ASB was defined as the growth of at least 10e5 colony forming units/ml of one organism or any presence of group B streptococcus (GBS) at the first urine culture collected during pregnancy without complaints of a UTI. A clinical UTI was diagnosed by the treating physician, in combination with a positive urine culture it was defined as culture-confirmed UTI. Results: No significant differences in prevalence of ASB (5.6% and 3.7%;relative risk (RR) 1.50;95% confidence intervals (CI) 0.44 - 5.17), incidence of clinical UTI (4.7% and 11.2%;RR 0.42;95% CI 0.15 - 1.14) or culture-confirmed UTI (2.8% and 3.7%;RR 0.75;95% CI 0.17 - 3.27) between pregnant women with and without (G)Dm were present. No association was found between ASB and UTI. GBS was the most common causative organism of ASB in women with and without Dm (66.7% and 50.0%). Conclusion: In contrast with earlier research, no significant differences in prevalence of ASB or incidence of UTI was found between pregnant women with and without (G)Dm.

关键词： Urinary Tract Infections Bacteriuria Diabetes mellitus Pregnancy Antenatal Screening

Who killed Porthos? Genetic tracking of a gorilla death

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Integrative Zoology 2007年第2期2卷 111-119页

作者： Kathryn J.JEFFERY Kate A.ABERNETHY caroline E.G.TUTIN Nicola A.ANTHONY michael W.BRUFORD Cardiff School of Biosciences Cardiff UniversityUK Centre International de Recherches médicales FrancevilleGabon Department of Biological and molecular Sciences University of StirlingStirlingUK

We describe a novel application of“forensic”genetics to study a key demographic event in a population of wild *** used microsatellite genotyping and non-invasively collected DNA samples to identify one individual western lowland gorilla as being most likely to have caused the death of another in LopéNational Park,*** from relatively few genotypes permitted the identification of female transfer events and the interpretation of individual behavior that was previously impossible using observational methods,thus providing rare behavioral data on an elusive forest-dwelling ***,this study highlights the need for future studies of dispersal and local population structuring in forest populations,and more accurate population census *** studies focusing on individual identification may play a valuable role in future gorilla conservation efforts.

关键词： ape conservation genetic tracking non-invasive microsatellite western lowland gorilla

Bone marrow Aspirate and Trephine Biopsy Procedure in a Teaching and Referral Hospital in Kenya

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Open Journal of Blood Diseases 2020年第4期10卷 124-132页

作者： Teresa Lotodo Beatrice melly Christopher m. Wanjiku caroline Kilachi Austin Omondi Japheth Kipkulei moi University Eldoret Kenya moi Teaching and Referral Hospital Eldoret Kenya AmPATH Oncology Eldoret Kenya Duke University Hospital Durham North Carolina

Introduction: Bone marrow aspiration and biopsies are carried out principally to permit cytological assessment of marrow. The procedure is also indicated for immunophenotypic, cytogenetic, molecular and other specialized investigations. The skill to perform bone marrow aspirate and biopsy is usually acquired by the medical personnel through apprenticeship during their training. The training includes performing the procedure, indications, contraindications, and associated hazards. moi Teaching and Referral Hospital (mTRH) being a specialized Hospital in Western Kenya has several staff trained on performing the procedure. Objective: To assess the performance of bone marrow procedure by clinicians at a teaching and referral hospital. materials and methods: A descriptive cross-sectional study was done involving 40 clinicians working in the hematology clinic and medical wards from June to December 2019. A structured questionnaire was used to collect the data and data analysis was done using frequency tables. Approval to carry out the study was sought from the institutional IRB. Results: Patients attended to were predominantly adults 33 (83%). The superior posterior iliac crest was the commonest site for the procedure and disposable needles were routinely used in 33 (83%) of the patients. Pain and excessive bleeding 18 (45%) were the commonest complications associated with the procedure. Conclusion: most of the clinicians involved doing the procedure were residents/registrars. most of the patients attended to were adults (83%) and majority of the clinicians performed the procedure on the posterior iliac site (96%) using disposable needles (83%). Local anesthesia was commonly used during the procedure (88%). The common complications associated with the bone marrow procedure were pain and excessive bleeding (45%).

关键词： Bone marrow Aspiration Bone marrow Needles Complications of Bone marrow Procedure Site for Bone marrow Procedure Trephine Biopsy

No benefit of flat head positioning in early moderate-severe acute ischaemic stroke: a HeadPoST study subgroup analysis

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Stroke & Vascular Neurology 2020年第4期5卷 406-409页

作者： Alejandro m Brunser menglu Ouyang Hisatomi Arima Pablo m Lavados Thompson Robinson Paula munoz-Venturelli Veronica V Olavarria Laurent Billot marre L Hackett Lili Song Sandy middleton Octavio Pontes-Neto Tsong-Hai Lee caroline Watkins Craig S Anderson Department of General Emergency Clinica Alemana de SantiagoClinica Alemana Universidad del DesarrolloSantiagoChile Department of Neurology and Psychiatry Clinica Alemana de SantiagoFaculty of MedicineClinica Alemana Universidad del DesarrolloSantiagoChile The George Institute for Global Health University of New South WalesSidneyNew South WalesAustralia The George Institute in China Peking University Health Science CenterBeijingChina Department of Preventive medicine and Public Health Fukuoka UniversityFukuokaJapan Department of Cardiovascular Sciences and NIHR Leicester Biomedical Research Centre University of LeicesterLeicesterUnited Kingdom Faculty of Health and Wellbeing University of Central LancashirePrestonLancashireUnited Kingdom Nursing Research Institute St Vincent's Health Network Sydney AustraliaAustralian Catholic UniversitySydneyNew South WalesAustralia Stroke Service-Neurology Division Department of Neuroscience and Behavioral SciencesRibeirao Preto Medical SchoolUniversity of Sao PauloSao PauloBrazil Stroke Center and Department of Neurology Linkou Chang Gung Memorial Hospital and College of MedicineChang Gung UniversityTaoyuanTaiwan

background Although the Head Positioning in acute Stroke Trial(HeadPoST)showed no effect of the flat head position(FP;vs sitting up head position(SUP))on functional outcome,we hypothesised that it could still offer benefits if commenced early in those with acute ischaemic stroke(AIS)of at least moderate *** Subgroup analysis of HeadPoST in participants with National Institutes of Health Stroke Scale(NIHSS)scores≥7,≥10 and≥14,randomised to FP or SUPmes defined by a shift in scores on the modified Rankin scale(mRS)and death/disability(mRS scores 3-6),and any cardiovascular serious adverse *** regression analyses were undertaken adjusted for study design and baseline risk *** There was no significant differential treatment effect in patient subgroups defined by increasing baseline NIHSS scores:adjusted OR and 95%CI for ordinal shift and binary(3-6)mRS scores:for NIHSS≥7(n=867)0.92(0.67 to 1.25)and 0.74(0.52 to 1.04);NIHSS≥10(n=606)0.80(0.58 to 1.10)and 0.77(0.49 to 1.19);NIHSS≥14(n=378)0.82(0.54 to 1.24)and 1.22(0.69 to 2.14).Conclusions Early FP had no significant effect in patients with moderate-severe AIS.

关键词： acute analysis effect

Chitosan coatings with distinct innate immune bioactivities differentially stimulate angiogenesis,osteogenesis and chondrogenesis in poly-caprolactone scaffolds with controlled interconnecting pore size

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Bioactive materials 2022年第4期7卷 430-442页

作者： caroline D.Hoemann Javier Rodríguez Gonzalez Jessica Guzman-morales Gaoping Chen Ebrahim Jalali Dil Basil D.Favis Department of Chemical Engineering Polytechnique MontrealQCCanada Department of Bioengineering George mason University VAUSA Centre de Recherche sur les Systemes Polymeres et Composites a Haute Performance(CREPEC) Canada

This study tested whether osseous integration into poly(ε-caprolactone)(PCL)bioplastic scaffolds with fully-interconnecting 155±8μm pores is enhanced by an adhesive,non-inflammatory 99% degree of deacetylation(DDA)chitosan coating(99-PCL),or further incorporation of pro-inflammatory 83% DDA chitosan microparticles(83-99-PCL)to accelerate *** Zealand White rabbit osteochondral knee defects were press-fit with PCL,99-PCL,83-99-PCL,or allowed to bleed(drill-only).Between day 1 and 6 weeks of repair,drill-only defects repaired by endochondral ossification,with an 8-fold higher bone volume fraction(BVF)versus initial defects,compared to a 2-fold(99-PCL),1.1-fold(PCL),or 0.4-fold(83-99-PCL)change in *** innate immune cells swarmed to 83-99-PCL,elicited angiogenesis throughout the pores and induced slight bone *** and 99-PCL pores were variably filled with cartilage or avascular mesenchyme near the bone plate,or angiogenic mesenchyme into which repairing trabecular bone infiltrated up to 1 mm *** repair cartilage covered the 99-PCL scaffold(65%)than PCL(18%)or 83-99-PCL(0%)(pmmatory chitosan coatings promoted cartilage infiltration into and over a bioplastic scaffold,and were compatible with trabecular bone *** study also revealed that in vitro osteogenesis assays have limited ability to predict osseous integration into porous scaffolds,because(1)in vivo,woven bone integrates from the leading edge of regenerating trabecular bone and not from mesenchymal cells adhering to scaffold surfaces,and(2)bioactive coatings that attract inflammatory cells induce bone resorption.

关键词： Bone tissue engineering Cartilage tissue engineering Hematoma Chitosan Angiogenesis Osteogenesis Inflammation

Effects of autoclaving on the physiological action of paclobutrazol

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Agricultural Sciences 2011年第3期2卷 191-197页

作者： Dimas mendes Ribeiro caroline müller Jackson Bedin Glayton Botelho Rocha Raimundo Santos Barros Depto de Biologia Vegetal/Universidade Federal de Vicosa VicosaBrazil

Besides being employed as an efficient plant growth retardant for field, garden and potted plants, paclobutrazol (PBZ) is also used in laboratory experiments, mainly in aseptic cultures, both in autoclaved and non-autoclaved form. Therefore it is not known if autoclaving can partial or completely inactivates the product, thus decreasing its efficacy. Thus a simple experiment was carried out to assess to what extent the autoclaving process can affect some growth components and dry mass accumulation and partition in sunflower seedlings, by employing the techniques of plant Growth Analysis. Autoclaving did not modify either qualitative or quantitatively any of the plant responses to PBZ as well their reversions by gibberellic acid.

关键词： Autoclaving Dry mass Accumulation Gibberilins Growth Paclobutrazol Sunflower

Adolescents’ perspectives on a school-based physical activity intervention:A mixed method study

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Journal of Sport and Health Science 2020年第1期9卷 28-40,F0003页

作者： Stephanie T.Jong caroline H.D.Croxson Cornelia Guell Emma R.Lawlor Campbell Foubister Helen E.Brown Emma K.Wells Paul Wilkinson Anna Vignoles Esther m.F.van Sluijs Kirsten Corder mRC Epidemiology Unit and UKCRC Centre for Diet and Activity Research(CEDAR) University of Cambridge School of Clinical MedicineCambridgeCB2 OQQUK Nuffield Department of Primary Care Health Sciences University of OxfordOxfordOX26GGU European Centre for Environment and Human Health University of Exeter Medical SchoolKnowledge SpaRoyal Cornwall HospitalTruroTRI 3HDUK Department of Psychiatry University of Cambridge and Cambridgeshire and Peterborough NHS Foundation TrustCambridgeCB215EFUK Faculty of Education University of CambridgeCambridgeCB28PQUK

Purpose'. To examine adolescent experiences and perspectives of the GoActive intervention (ISRCTN31583496) using mixed methods processevaluation to determine satisfaction with intervention components and interpret a*dolescents experiences of the intervention process in order toprovide insights for future intervention ***'. Participants (n = 1542;13.2 土 0.4 years, mean 土 SD) provided questionnaire data at baseline (shyness, activity level) and post-intervention(intervention acceptability, satisfaction with components). Between-group differences (boys vs. girls and shy/inactive vs. others) weretested with linear regression models, accounting for school clustering. Data from 16 individual interviews (shy/inactive) and 11 focus groupswith 48 participants (mean = 4;range 2—7) were thematically coded. Qualitative and quantitative data were merged in an integrative mixedmethods convergence matrix, which denoted convergence and dissonance across ***'. Effect sizes for quantitative results were small and may not represent substantial between-group differences. Boys (vs. girls) preferredclass-based sessions (0 = 0.2, 95% confidence interval (CI): 0.1—0.3);qualitative data suggested that this was because boys preferred competition,which was supported quantitatively (0 = 0.2, 95%CI: 0.1-0.3). Shy/inactive students did not enjoy the competition (0 = -0.3, 95%CI:—0.5 to —0.1). Boys enjoyed trying new activities more (0 = 0.1, 95%CI: 0.1 -0.2);qualitative data indicated a desire to try new activities acrossall subgroups but identified barriers to choosing unfamiliar activities with self-imposed choice restriction leading to boredom. Qualitative datahighlighted critique of mentorship;adolescents liked the idea, but older mentors did not meet ***. We interpreted adolescent perspectives of intervention components and implementation to provide insights into future complexinterventions aimed at increasing young people's physical activity in s

关键词： Adolescent Intervention mixed methods Physical activity Process evaluation

The transcription factor HSFA7b controls thermomemory at the shoot apical meristem by regulating ethylene biosynthesis and signaling in Arabidopsis

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Plant Communications 2024年第3期5卷 47-61页

作者： Sheeba John Federico Apelt Amit Kumar Ivan FAcosta Dominik Bents maria Grazia Annunziata Franziska Fichtner caroline Gutjahr Bernd mueller-Roeber Justyna J.Olas University of Potsdam Institute of Biochemistry and BiologyKarl-Liebknecht-Straße 24-25Haus 2014476 PotsdamGermany max Planck Institute of molecular Plant Physiology Am Muehlenberg 114476 PotsdamGermany Laboratory of molecular Biology Wageningen University6700 AP Wageningenthe Netherlands Center of Plant Systems Biology and Biotechnology(CPSBB) 14 St.Knyaz Boris 1 Pokrastitel Str.4023 PlovdivBulgaria Present address:Institute of Plant Biochemistry Heinrich Heine University DusseldorfUniversit€atsstr.140225 DusseldorfGermany Leibniz Institute of Vegetable and Ornamental Crops Theodor-Echtermeyer-Weg 114979 GroßbeerenGermany

The shoot apical meristem(SAm)is responsible for overall shoot growth by generating all aboveground *** research has revealed that the SAm displays an autonomous heat stress(HS)memory of a previous non-lethal HS *** the importance of the SAm for plant growth,it is essential to determine how its thermomemory is mechanistically ***,we report that HEAT SHOCK TRAN-SCRIPTION FACTOR A7b(HSFA7b)plays a crucial role in this process in Arabidopsis,as the absence of functional HSFA7b results in the temporal suppression of SAm activity after *** found that HSFA7b directly regulates ethylene response at the SAm by binding to the promoter of the key ethylene signaling gene ETHYLENE-INSENSITIVE 3 to establish ***,we demonstrated that HSFA7b regulates the expression of ETHYLENE OVERPRODUCER 1(ETO1)and ETO1-LIKE 1,both of which encode ethylene biosynthesis repressors,thereby ensuring ethylene homeostasis at the *** together,these results reveal a crucial and tissue-specic role for HSFA7b in thermomemory at the Arabidopsis SAm.

关键词： ethylene response heat stress adaptation shoot apical meristem SAm thermopriming thermomemory